FastQCFastQC Report
Tue 19 Sep 2017
sim-trimmed-pair1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamesim-trimmed-pair1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length48-50
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT450.001398044824.44444517
TCACTTG3250.00778552056.76923120
Produced by FastQC (version 0.11.5)