FastQCFastQC Report
Fri 19 Feb 2016
WNN1_6433_read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN1_6433_read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40324980
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG55500.024.008362
ATACCGC69750.020.2009983
ACCGACG87950.019.08258821
CGACGAA87750.018.95838223
CGTTGTA97150.018.7142235
GCCTAAC101750.018.45931
TCTACAC130300.018.3865783
CCGACGA92600.018.07839222
TCCGCAA76500.017.86071833
CGCCATC76350.017.6245167
TACACGG43700.017.5235945
CCGCCAT76850.017.28156
TACGCCG31700.017.1860735
GTCGTAT30600.016.96838625
TCGTATA31650.016.80059826
CGCCCGT100200.016.72575610
GCCCGTC102550.016.4275711
CGCGGTA23400.016.15521843
CCGTCAC106200.016.11004413
CCGCAAA90400.015.97167834
Produced by FastQC (version 0.11.2)