FastQCFastQC Report
Fri 19 Feb 2016
WNN1_6433_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN1_6433_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40324980
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG18750.028.5552371
TAGGACG40650.028.4194564
TACGCGT22050.027.407185
ATACGCG24000.025.6722434
GTAGGAC58600.023.755423
TATACGC24400.021.6069093
CTGTAGG69150.021.106931
GGACGTG48300.019.5097186
TTATACG26450.018.3032132
GTACGAG14900.018.11175519
CGCGTAT27700.018.1071917
ACGCGTA28950.017.870536
GCGTATA22350.017.392521
GGTACGA15600.017.36709618
GTCCTAC57750.017.2723371
CTAGACT82950.016.8553314
GTTATAC40950.016.8359681
GACGTGG32350.016.7284937
TAGACGC26050.016.6018644
GACGGGC20100.016.506875
Produced by FastQC (version 0.11.2)