FastQCFastQC Report
Wed 31 Aug 2016
CD03_15471_TCAGAG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCD03_15471_TCAGAG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43634602
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACTCAGAGATCTCGTATG2867970.6572696595238797Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA319700.041.9695432
ATCGGAA321400.041.720653
CTCGTAT315750.041.13479243
TCGTATG317100.040.9527344
AGATCGG326900.040.9489141
TCTCGTA319200.040.69708342
CGTCAGA328100.040.1230717
ACACGTC332900.039.65666214
ATCTCGT329200.039.4011541
TCGGAAG339700.039.388924
ACGTCAG336050.039.2131516
CGGAAGA341950.039.0436555
CACGTCA340250.038.83902415
CACACGT364700.036.2892813
GCACACG379750.035.03646512
TCAGAAC408650.032.44056319
GTCACTC411950.031.80145630
GAGATCT415050.031.55885138
AGATCTC413950.031.54694439
AGAACTC419300.031.5221521
Produced by FastQC (version 0.11.2)