FastQCFastQC Report
Wed 31 Aug 2016
CD04_15472_CACAGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCD04_15472_CACAGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58582903
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACCACAGTATCTCGTATG3683430.6287551164885086TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA411500.041.877142
ATCGGAA416300.041.3631823
AGATCGG418250.041.0514451
CTCGTAT408100.040.8016143
TCGTATG408150.040.6456944
TCTCGTA411000.040.55653442
TATCTCG417650.040.03223440
CGTCAGA424500.039.98771717
ACACGTC428700.039.71850214
TCGGAAG437150.039.329964
ATCTCGT426600.039.14076241
ACGTCAG434550.039.1033716
CGGAAGA441900.038.8960535
CACGTCA438800.038.7998415
CACACGT471800.036.2113613
CAGTATC480050.035.03046437
GTATCTC481550.034.86628739
GCACACG491550.034.83250412
AGTATCT523950.032.1289738
ACAGTAT533000.031.71983736
Produced by FastQC (version 0.11.2)