FastQCFastQC Report
Wed 31 Aug 2016
LB01_15473_GAGTCA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLB01_15473_GAGTCA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49072566
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGAGTCAATCTCGTATG2178630.44396088845241966TruSeq Adapter, Index 9 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT254200.039.85339443
TCTCGTA256750.039.52612342
ACGAGTC259150.039.4233133
TCGTATG258150.039.14132344
CGTCAGA267200.039.03438617
GATCGGA275450.038.320592
CGAGTCA267100.038.24167334
ACACGTC273700.038.21163614
GTCACGA271400.038.09774430
ATCGGAA277950.037.8970033
ATCTCGT269550.037.7226241
ACGTCAG276850.037.6975916
CACGAGT273250.037.590332
AGATCGG278700.037.528881
AGTCACG276450.037.41771329
CACGTCA282950.036.9704115
TCGGAAG290150.036.3122674
GTCAATC281450.036.03394337
CGGAAGA297950.035.4505125
CACACGT309600.033.951313
Produced by FastQC (version 0.11.2)