FastQCFastQC Report
Wed 31 Aug 2016
LB03_15475_TCAGAG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLB03_15475_TCAGAG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57816032
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACTCAGAGATCTCGTATG1366700.23638772027800178Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA173400.038.949832
CTCGTAT171300.038.32241443
AGATCGG177650.037.885241
TCTCGTA173700.037.7802542
ATCGGAA181050.037.2312853
TCGTATG176750.037.1532144
CGTCAGA179950.036.9696617
ACACGTC184350.036.30174314
ATCTCGT184950.035.4821841
ACGTCAG189100.035.3436216
TCGGAAG191150.035.287684
CACGTCA195150.034.19153615
CGGAAGA199850.033.817895
GCACACG224300.030.15971612
CACACGT226600.029.6885513
GTCACTC279700.023.70621330
GATCTCG288800.023.17252740
AGAGCAC310750.022.0178539
GAGATCT305350.021.86614238
AGATCTC313350.021.30788839
Produced by FastQC (version 0.11.2)