FastQCFastQC Report
Wed 31 Aug 2016
LB04_15476_CACAGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLB04_15476_CACAGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62003249
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACCACAGTATCTCGTATG4611510.7437529604295413TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2459440.39666308454255356No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1708060.2754791123929651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT521000.040.84048543
TCGTATG519500.040.8186644
TCTCGTA526600.040.46466442
GATCGGA548650.040.1818662
ATCGGAA549450.040.1396683
CGTCAGA547600.039.7930217
TATCTCG539400.039.61455540
ACACGTC553900.039.4989714
AGATCGG554650.039.393331
ATCTCGT549500.038.8704141
ACGTCAG561700.038.8254216
CACGTCA566750.038.51828815
TCGGAAG576900.038.200014
CGGAAGA579050.038.1078455
GTATCTC580500.036.89317739
CAGTATC584150.036.7417437
CACACGT600950.036.46505713
ACAGTAT611250.035.2459536
AGTATCT616800.034.77938
GCACACG637400.034.5385612
Produced by FastQC (version 0.11.2)