FastQCFastQC Report
Fri 20 Mar 2020
IVT.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameIVT.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3460914
Sequences flagged as poor quality0
Sequence length1-56317
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAAT64230.1855868131944336No Hit
TTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAA60040.17348018471421134No Hit
TGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCC57660.166603388584634No Hit
TTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAAT54640.15787736996643084No Hit
TCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAA52170.1507405269243905No Hit
TGGTATGATTTCGGTGATTCATACAAACCACGCCAGGTAGTGGAGTTCCT39230.11335155973248685No Hit
TGGTATGATTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCT37900.10950864424831128No Hit
AGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG35110.10144718996195803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)