| General summary |
Day2_1 |
Day2_2 |
Day2_3 |
Day5_1 |
Day5_2 |
Day5_3 |
Day9_1 |
Day9_2 |
Day9_3 |
Day15_1 |
Day15_2 |
Day15_3 |
| Average percent identity |
88.0 |
86.7 |
86.9 |
88.3 |
87.7 |
89.2 |
86.6 |
89.1 |
88.2 |
84.4 |
84.3 |
85.3 |
| Fraction of bases aligned |
0.9 |
0.9 |
0.9 |
0.9 |
0.9 |
0.9 |
0.9 |
0.9 |
0.9 |
0.9 |
0.9 |
0.9 |
| Mean read length |
709.5 |
975.7 |
1,092.6 |
1,231.6 |
940.0 |
551.4 |
1,059.3 |
748.6 |
1,049.5 |
764.9 |
827.3 |
677.4 |
| Mean read quality |
10.8 |
11.0 |
11.2 |
11.4 |
10.9 |
10.3 |
10.8 |
10.7 |
11.3 |
10.7 |
10.7 |
10.3 |
| Median percent identity |
89.3 |
88.4 |
88.6 |
89.8 |
89.3 |
90.3 |
88.5 |
90.1 |
89.7 |
86.7 |
86.5 |
87.4 |
| Median read length |
486.0 |
718.0 |
868.0 |
976.0 |
691.0 |
340.0 |
821.0 |
514.0 |
823.0 |
577.0 |
684.0 |
458.0 |
| Median read quality |
10.8 |
11.0 |
11.3 |
11.7 |
11.0 |
10.3 |
10.8 |
10.8 |
11.5 |
10.7 |
10.8 |
10.2 |
| Number of reads |
367,760.0 |
255,749.0 |
390,024.0 |
458,735.0 |
352,303.0 |
142,405.0 |
581,331.0 |
430,745.0 |
692,071.0 |
159,750.0 |
101,437.0 |
261,864.0 |
| Read length N50 |
1,109.0 |
1,579.0 |
1,683.0 |
1,809.0 |
1,446.0 |
844.0 |
1,725.0 |
1,186.0 |
1,723.0 |
1,094.0 |
1,114.0 |
943.0 |
| STDEV read length |
631.8 |
855.5 |
883.6 |
953.8 |
832.8 |
514.9 |
899.6 |
711.2 |
895.7 |
656.0 |
656.9 |
621.0 |
| Total bases |
260,913,383.0 |
249,545,711.0 |
426,141,282.0 |
564,993,679.0 |
331,173,076.0 |
78,518,773.0 |
615,828,706.0 |
322,446,657.0 |
726,343,193.0 |
122,198,537.0 |
83,916,096.0 |
177,388,947.0 |
| Total bases aligned |
231,201,143.0 |
227,048,420.0 |
390,953,942.0 |
527,786,438.0 |
300,208,056.0 |
67,624,617.0 |
564,491,200.0 |
288,917,896.0 |
675,478,952.0 |
108,697,833.0 |
75,641,161.0 |
155,924,823.0 |
| Number, percentage and megabases of reads above quality cutoffs |
|
|
|
|
|
|
|
|
|
|
|
|
| >Q5 |
367398 (99.9%) 260.8Mb |
255646 (100.0%) 249.5Mb |
389821 (99.9%) 426.1Mb |
458293 (99.9%) 564.9Mb |
351833 (99.9%) 331.0Mb |
142035 (99.7%) 78.4Mb |
580965 (99.9%) 615.7Mb |
430188 (99.9%) 322.3Mb |
691487 (99.9%) 726.2Mb |
159664 (99.9%) 122.2Mb |
101328 (99.9%) 83.9Mb |
261541 (99.9%) 177.2Mb |
| >Q7 |
356716 (97.0%) 257.7Mb |
250834 (98.1%) 247.7Mb |
381461 (97.8%) 423.0Mb |
445561 (97.1%) 560.8Mb |
340626 (96.7%) 327.3Mb |
134983 (94.8%) 76.8Mb |
563387 (96.9%) 609.7Mb |
413625 (96.0%) 318.3Mb |
672136 (97.1%) 720.3Mb |
156060 (97.7%) 120.9Mb |
98494 (97.1%) 82.9Mb |
252203 (96.3%) 174.5Mb |
| >Q10 |
235200 (64.0%) 213.3Mb |
167549 (65.5%) 212.0Mb |
269639 (69.1%) 368.0Mb |
329660 (71.9%) 503.9Mb |
226754 (64.4%) 278.5Mb |
79457 (55.8%) 59.9Mb |
359783 (61.9%) 516.7Mb |
264095 (61.3%) 269.9Mb |
484863 (70.1%) 640.9Mb |
98541 (61.7%) 98.0Mb |
64495 (63.6%) 67.2Mb |
140174 (53.5%) 134.1Mb |
| >Q12 |
108641 (29.5%) 134.7Mb |
89801 (35.1%) 149.7Mb |
146528 (37.6%) 258.3Mb |
203546 (44.4%) 382.9Mb |
115476 (32.8%) 189.4Mb |
32062 (22.5%) 34.5Mb |
188791 (32.5%) 355.5Mb |
131818 (30.6%) 183.7Mb |
297145 (42.9%) 490.0Mb |
42489 (26.6%) 58.1Mb |
26575 (26.2%) 37.2Mb |
53392 (20.4%) 72.9Mb |
| >Q15 |
2827 (0.8%) 5.6Mb |
4696 (1.8%) 11.8Mb |
7040 (1.8%) 18.2Mb |
9695 (2.1%) 26.6Mb |
4124 (1.2%) 10.4Mb |
703 (0.5%) 1.2Mb |
6640 (1.1%) 18.4Mb |
4505 (1.0%) 10.7Mb |
19074 (2.8%) 48.6Mb |
965 (0.6%) 2.1Mb |
379 (0.4%) 0.9Mb |
1133 (0.4%) 2.3Mb |
| Top 5 highest mean basecall quality scores and their read lengths |
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|
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| 1 |
19.7 (2286; 4ca53d22-9746-4dae-996a-5c26f9143d0c) |
19.0 (3646; 83d5d042-408d-42ea-ae76-c5a32da31587) |
20.2 (4234; 11d871a4-8352-44ff-a07e-9a617b0baba4) |
19.2 (1643; dd7481c9-79de-44e4-a80b-272d7c4e8866) |
18.9 (1619; 3048ded5-3a4c-4ab0-ad3c-8348774ab448) |
20.3 (182; 8b667956-17e0-44a1-8cfb-4efc3e2ba7bb) |
18.8 (4096; 473c1e3b-9971-4e67-adbb-cb014abecad5) |
18.9 (1887; 2e0e8485-0304-43b1-9953-fadfb6d1d3b3) |
19.2 (1532; 10d313cf-465a-489e-b994-2f4b078b6257) |
18.3 (2918; 2c8fb5f3-a167-4285-8d91-0dc19d37efa5) |
17.1 (1812; 9d125e73-c083-4d49-b790-3d72fa68b7a4) |
18.4 (1901; ac82c14d-b8c2-4a43-98eb-9a352b1751d0) |
| 2 |
19.7 (2408; 000259b2-7c72-443f-9a1f-d9010fc3a672) |
19.0 (1903; c6da01a3-2b2d-4671-8611-4a59ecf73478) |
18.9 (3314; 626774b5-07ad-4729-a897-a8ff28ca383d) |
19.0 (5906; 362b6b7a-fe2a-40a1-8025-ae3731526e65) |
18.7 (899; 80deda4c-cc2c-4e6d-8713-ed17cde1b19a) |
19.5 (609; 406091f9-5cce-40da-9f67-8cf1a74f6da4) |
18.4 (996; 022d7355-7b9a-4d13-b4d6-3f10bc5bba5b) |
18.9 (2884; fc8710c0-161d-4aca-a98b-9ae26eee1b7e) |
19.1 (1635; 611106c2-5583-4e13-b075-431575b0e685) |
18.0 (1000; 51f9b2f4-1846-4b00-9590-ec49b80434be) |
17.0 (1010; 123e9299-3111-464b-bc01-f44dd2db4ddc) |
17.5 (1052; a1de4cbc-9d05-4245-9e9a-b19b97d4519b) |
| 3 |
19.4 (4010; 12f5d855-ec09-4d02-972d-b50e07b4ec32) |
18.7 (2251; b4b22509-5d3e-4dbc-a994-73309208f53e) |
18.7 (2011; 3946cfd4-7c8b-4ee7-bf14-8872d9bcd475) |
18.8 (2623; 5b57c674-5c0b-46f3-819d-9719960e56e7) |
18.4 (1048; 3f48d480-0f8d-4ffb-a6e0-1e49eb5725eb) |
18.4 (2244; 7120ed00-d829-47e6-b6fe-90477c0f2697) |
18.4 (2930; c6cecd5e-f8a3-4366-b147-601b963957aa) |
18.8 (1992; 804a850e-b6b7-44bb-9ad8-849c2d0960c1) |
19.1 (2249; e7dec4d3-c3f0-452e-96a4-115d70c9dd94) |
18.0 (4093; 37872dd9-58e7-4142-bc62-80f56eaa2649) |
16.9 (1002; 122b53ca-557c-4af9-b05d-d013a9a00c81) |
17.5 (1213; 9ffe7b82-da13-4a09-966d-99b982bd8dbf) |
| 4 |
18.4 (1017; 851c0c0b-d707-474e-8f4d-72ddaacd1021) |
18.4 (1374; eab4d2c0-71fb-4f58-bbda-d4f5f8fe76a9) |
18.7 (1721; 355204d2-8bf4-481d-8240-af45c53c9e38) |
18.2 (2123; b6248340-02fc-4d20-a96a-8b6df2e6148e) |
18.3 (2343; 4c74afe5-1399-4493-b8c3-1b3f3eaecd3d) |
18.2 (72; c471f8a3-fa63-4d9d-85fc-f118188b4896) |
18.2 (990; ea92c0d5-34b1-4b1b-9ded-8bbd1667421c) |
18.8 (3089; 2be3854e-7483-48f9-9dc6-514ee8e7cf3c) |
19.0 (2579; 258a9fc1-b05f-4438-9aca-7337fa4a5155) |
17.9 (1876; 9be71228-8b70-46a8-9a20-9233e6bc4ffb) |
16.9 (983; ad93b695-c811-4105-be9d-d803a72b30a5) |
17.4 (1848; c99312d5-f623-4742-80ad-1e2cdc6eaef4) |
| 5 |
18.3 (1107; 25f1aa9c-b741-4cea-947f-cf41e37eca04) |
18.4 (3095; 48eb6108-fabf-4647-9f6c-53de5ab05df2) |
18.6 (2899; 63114be0-455b-4617-9b55-9f02e38d09d4) |
18.1 (1629; a2e2c03d-63b5-4362-a46b-cb4716632881) |
18.2 (2713; 157b0ce8-c756-4aa9-81df-4ce2db58f6e5) |
18.1 (836; 68b86af0-32fa-45ba-9b31-b1f66e7c84fd) |
18.0 (2263; d6b2171a-f39c-442d-ab07-18feace6dac0) |
18.6 (2361; 44f83271-5b53-42d9-87bf-c671a6a7b8a7) |
19.0 (1274; 293eabdf-736a-4a6f-89b0-9a5dec3b9715) |
17.8 (1034; 9a843dab-b9d9-4acb-a6d9-01577d45b754) |
16.9 (358; 94bdeb13-b7e9-45c2-b862-a946523ba641) |
17.3 (1719; a5af7c1e-9eee-451a-a741-d6c1fe0518b6) |
| Top 5 longest reads and their mean basecall quality score |
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| 1 |
13356 (4.7; fa262aac-0ba0-492a-b20f-f926ad3b4882) |
5059 (11.7; 27f4c7bd-6a57-499b-bdfb-c4e334fc2c01) |
4934 (16.6; f12e987f-cec0-4fb6-9cdd-b5f0e2b20855) |
6503 (14.4; 23b277d9-c882-4e5a-853f-94782ab356c2) |
48799 (7.0; 20d82a9c-661a-4712-b1a1-d0c936e4f861) |
4115 (15.5; 888f8fe4-5f08-40fa-b736-ae06832d247d) |
12526 (1.7; 54a45dc6-e98d-4634-9048-7a15fb4ab8e1) |
6305 (1.8; 84fff710-cc43-41da-b197-071f9ef513d8) |
5987 (14.9; 883cdea3-d8a3-4282-898d-a0027f44fd1c) |
4496 (2.1; e78d6815-dd7a-4941-9ead-31a32e46c348) |
3851 (9.4; 9d110097-f090-43a3-8d22-47e55a7efb12) |
44522 (5.8; 69dfb574-aaf2-45f2-a601-469755f9ec79) |
| 2 |
5178 (13.2; 442d364a-c17c-4d92-9634-853634801516) |
4935 (17.2; dc316f41-b22c-43d6-b21c-32a39a7a0c3c) |
4866 (13.4; 532ed5d8-aa57-49b2-bdcf-cd12b68e2b64) |
5908 (4.4; c4afd524-5c7d-49bc-8342-4e79c204d9da) |
31382 (1.2; 89907037-ec19-4f79-ad6b-d948e14285f5) |
4114 (14.1; 2eb717ff-413f-407b-94b0-6f9e7dfe466e) |
7938 (4.4; ced4248e-9816-4517-8a23-290f701ef15b) |
5365 (17.2; 8eea6ec4-b6cd-4f27-964c-89a355a8ba8a) |
5325 (14.0; 9034d526-65c8-480c-a375-e0457b763c81) |
4093 (18.0; 37872dd9-58e7-4142-bc62-80f56eaa2649) |
3781 (8.5; 292bbae3-e809-48f6-885c-1fda8948ed23) |
32546 (4.4; f1a628d4-8208-41c0-b3af-5286c8e334c9) |
| 3 |
4400 (13.9; e4e0deeb-27c4-499d-8ef9-7aeb26d26e9d) |
4757 (17.2; 7ee698dc-5cc5-4a34-82e7-dcbc16623823) |
4767 (11.5; bb4673c0-34a5-4606-a798-cd7dc79fd074) |
5906 (19.0; 362b6b7a-fe2a-40a1-8025-ae3731526e65) |
5570 (1.5; b9957921-53c8-4830-bfc0-1b13d3b4240a) |
4079 (15.3; 44ad47cd-6479-4a65-8d0c-5c89e00b9ade) |
5005 (14.3; e0a1c0af-43d3-4d92-bbd9-ff05a561fbf8) |
5350 (13.9; 8b8f3535-19a9-4e14-9476-74d5727746bd) |
5117 (14.2; 189cde26-dac2-4466-bc78-9908f65f0766) |
4000 (13.2; e0e4c280-b115-4add-80cf-059b2aa4cbe7) |
3708 (10.3; b3a8435d-6eec-48cd-8d58-2d54d0dc235f) |
14907 (4.3; d0fa2554-597a-4b8e-9e61-0d46b2c583d3) |
| 4 |
4263 (14.4; e8210e10-60e1-41b4-9d59-eb70e38f4f63) |
4482 (10.5; b6791099-1289-4bdb-8624-2a99c647e00f) |
4688 (9.0; c1019c65-14d1-4cb1-b698-da1a75a6caf1) |
5409 (13.3; 3ba15c14-6696-4e3e-9178-7374b48690c1) |
4528 (15.5; e6ed2911-dac7-4cfd-8dce-4421942d545d) |
4074 (12.9; 116aef52-62d4-42f1-a27c-27dbc36ac6e7) |
4880 (13.3; 86eaeb30-7191-48c6-a2f8-368cbc141c7f) |
5343 (13.4; 2d7cd2ef-5e24-4efe-8a73-bb27c3d890b4) |
5001 (4.7; ae978f07-afb0-40dd-b71f-f18376e19ec6) |
3753 (13.5; 452a554f-f520-4961-a85b-0b9e9ebf1096) |
3707 (13.8; da969796-d22d-498d-b309-ccb679e1ab8a) |
9313 (2.1; 835affbc-483f-401d-84ea-7378dd52e1d3) |
| 5 |
4071 (14.5; cc7b4469-17bb-456c-bfb8-5a634176d056) |
4296 (15.4; ab79b59c-974e-40cc-aeb9-e3c525473b65) |
4534 (16.2; b99402cf-cd2b-4e47-915f-c6d5df409259) |
5277 (11.7; 2f9e5b41-ef1d-497c-8b94-b21902734f2d) |
4140 (14.3; 5005d73b-ed9b-4de8-aa28-31031f7a84f0) |
3805 (14.5; cc988389-ce2f-4042-a701-a4d1c18d4f75) |
4437 (8.1; 44b86e94-d92e-47b3-9959-3b061979e18f) |
5163 (3.6; 94d69012-a5d4-4195-a9a3-ce8e32278c66) |
4850 (15.5; 315c92dc-745a-4ffb-9176-d430a2c7d84e) |
3747 (9.1; 9e9a3fd1-d9d1-45a3-bef5-9d9488a896ae) |
3698 (10.7; fe20fbe8-6022-4f68-8b17-e5be853df0d1) |
8222 (4.8; 1bbfd608-40a2-4957-91d6-5024861ae1c8) |