# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/EMG1.hmm.txt # target sequence database: proteomes/Thermococcus_kodakarensis.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: EMG1 [M=194] Accession: PF03587.13 Description: EMG1/NEP1 methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-51 172.5 0.0 1.2e-51 172.3 0.0 1.0 1 sp|Q5JI44|NEP1_THEKO Ribosomal RNA small subunit methyltrans Domain annotation for each sequence (and alignments): >> sp|Q5JI44|NEP1_THEKO Ribosomal RNA small subunit methyltransferase Nep1 OS=Thermococcus kodakarensis (strain ATCC BA # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 172.3 0.0 5e-55 1.2e-51 2 194 .] 5 215 .. 4 215 .. 0.93 Alignments for each domain: == domain 1 score: 172.3 bits; conditional E-value: 5e-55 EEEEES--B--GG................SCGGSB--HHHHCGG.TTSTTGGGSS-CHHHHHHHHHHHCSCHHCTT-EEEEEEETTSEE CS EMG1 2 vLeeasLelvkvk................kkseqLLnsddhkkilkklkreakeaRpDIvHqcLlalldspLnkagllqvyihttknvl 74 +++ea+Lelv+++ +++e +L+s++h+ +lkkl + +++RpDIvH cLl++l+s+ nk+g l+vy+ht+++++ sp|Q5JI44|NEP1_THEKO 5 IIAEAELELVPESirdhpavvnyarrrkkRPEEAILDSTYHHAALKKLPDGDRRGRPDIVHICLLNALESIANKEGFLRVYVHTRNDEV 93 899*********99**************99*********************************************************** PP EEE-TT----SSHHHHHHHHHHHHCCSEEEETTEE.SCEEEECS-HHHHCCC..-SEEEEE-TTSEE-..................EEE CS EMG1 75 IevnpqtRiPrtfkrFagLmvqLLkklsirategeekLlkviknpitdhlpp..gskiillsesaekklvdleefv.....vkpvvfvi 156 I+++p+tR+Pr+++rF gLm++L+kk+ +++ +Ll++ k+p+++++++ ++++++e++e l+++++f +++ v+v+ sp|Q5JI44|NEP1_THEKO 94 IHIKPETRLPRNYNRFLGLMESLFKKGAVPEGL---ELLRIEKKPLESLIEDinPDTVFIMHEEGE--LIRPRSFGeilasHQNPVVVV 177 *****************************9864...69*************988899*********..66677776667886677799* PP E-SSSS....---HHEEESSSS---HHHHHHHHHHHCH CS EMG1 157 gamskgsvkidykeeeisisnypLsaavvcskiltafE 194 g++++g+ ++ +++s++++pL a++v+s+++ +fE sp|Q5JI44|NEP1_THEKO 178 GGFPHGDFMRPIEGTKVSLYREPLMAWTVVSEVIVNFE 215 ******555556667*******************9998 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (194 nodes) Target sequences: 2301 (637680 residues searched) Passed MSV filter: 76 (0.0330291); expected 46.0 (0.02) Passed bias filter: 64 (0.027814); expected 46.0 (0.02) Passed Vit filter: 7 (0.00304216); expected 2.3 (0.001) Passed Fwd filter: 1 (0.000434594); expected 0.0 (1e-05) Initial search space (Z): 2301 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 12370.99 // [ok]