# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/FtsJ.hmm.txt # target sequence database: proteomes/Ciona_savignyi.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: FtsJ [M=177] Accession: PF01728.18 Description: FtsJ-like methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.3e-67 226.7 0.1 1.6e-67 226.4 0.1 1.1 1 tr|H2ZIK3|H2ZIK3_CIOSA Putative tRNA (cytidine(32)/guanosine 1.4e-54 184.2 0.1 2.9e-54 183.3 0.1 1.5 1 tr|H2YL40|H2YL40_CIOSA Putative rRNA methyltransferase OS=Ci 5e-05 22.9 0.1 9e-05 22.0 0.1 1.7 1 tr|H2Z203|H2Z203_CIOSA Uncharacterized protein OS=Ciona savi 0.0005 19.6 0.0 0.0017 17.9 0.0 1.9 2 tr|H2YUK6|H2YUK6_CIOSA Uncharacterized protein OS=Ciona savi ------ inclusion threshold ------ 0.01 15.3 0.0 0.02 14.4 0.0 1.5 1 tr|H2YGP5|H2YGP5_CIOSA Uncharacterized protein OS=Ciona savi 0.069 12.6 0.0 0.098 12.1 0.0 1.3 1 tr|H2YFA0|H2YFA0_CIOSA Uncharacterized protein OS=Ciona savi 0.072 12.6 0.0 0.16 11.5 0.0 1.6 1 tr|H2YC22|H2YC22_CIOSA Uncharacterized protein OS=Ciona savi 0.098 12.1 0.4 0.22 11.0 0.3 1.5 1 tr|H2Z8K9|H2Z8K9_CIOSA Uncharacterized protein OS=Ciona savi Domain annotation for each sequence (and alignments): >> tr|H2ZIK3|H2ZIK3_CIOSA Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Ciona savignyi OX=51511 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 226.4 0.1 1.1e-70 1.6e-67 1 177 [] 21 212 .. 21 212 .. 0.94 Alignments for each domain: == domain 1 score: 226.4 bits; conditional E-value: 1.1e-70 TTSHHHHHHHHHHHHH--S-.TT-EEEEES-TT-HHHHHHTTT...............-SEEEEEESS-----TT-EEEE--TTSSS CS FtsJ 1 yrsRsayKLleidekfklfk.pgktvvDlgaaPGgwsqvllqrl............gakgkvvavDlqpmaplpgvttiqgditdpe 74 +r+Rsa+KLl++de+f+lf+ +k+vvDl+aaPG+wsqvl+++l +++ k+vavDlq ma lpgv ++qgdit+ + tr|H2ZIK3|H2ZIK3_CIOSA 21 WRARSAFKLLHLDEEFNLFHnGVKKVVDLCAAPGSWSQVLSRKLrflialgsgkeeNKDVKIVAVDLQAMASLPGVIQLQGDITNVS 107 79*****************956689*******************999999887664335**************************** PP HHHHHHHHHHHSSEEEEEE-------S-HHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEE-..STTHHHHHHHHGGGEEEEEEE- CS FtsJ 75 tiekikealserkvdlvvsDgapdvsgvhdldeylqsqLllaalelalkllkpggtfvlkifr..geeteallkqlkkfFekvsvvk 159 t+e+i +++++ +dlvv+Dgapdv+g hd dey+q+qLllaal++++++lk+ggtfv+kifr g++++ l+ ql+ fF kv+++k tr|H2ZIK3|H2ZIK3_CIOSA 108 TAEAIISHFEGESADLVVCDGAPDVTGLHDMDEYIQAQLLLAALNITTHVLKNGGTFVAKIFRgkGKDVTLLYDQLRLFFTKVTCAK 194 ***************************************************************777777788888************ PP ---------EEEEEEEEE CS FtsJ 160 Pkssrsesseeflvcrgf 177 P+ssr++s e+f+vc+++ tr|H2ZIK3|H2ZIK3_CIOSA 195 PRSSRNSSIEAFVVCENY 212 ***************986 PP >> tr|H2YL40|H2YL40_CIOSA Putative rRNA methyltransferase OS=Ciona savignyi OX=51511 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 183.3 0.1 2e-57 2.9e-54 1 177 [] 24 200 .. 24 200 .. 0.99 Alignments for each domain: == domain 1 score: 183.3 bits; conditional E-value: 2e-57 TTSHHHHHHHHHHHHH--S-TT-EEEEES-TT-HHHHHHTTT...-SEEEEEESS-----TT-EEEE--TTSSSHHHHHHHHHHHSS CS FtsJ 1 yrsRsayKLleidekfklfkpgktvvDlgaaPGgwsqvllqrlgakgkvvavDlqpmaplpgvttiqgditdpetiekikealserk 87 yr+Rsa+KLl++++kf++++ +++vDl+aaPGgw qv+++++ ++ +v+vDl p++p+p++ t+q dit+++ + ++++ l++ k tr|H2YL40|H2YL40_CIOSA 24 YRARSAFKLLQLNKKFNFLQSSQACVDLCAAPGGWLQVASEHMPMSSIIVGVDLVPIRPVPKCVTFQDDITKESCRTQLRRELHQWK 110 9****************99************************889***************************************** PP EEEEEE-------S-HHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEE-STTHHHHHHHHGGGEEEEEEE----------EEEEEE CS FtsJ 88 vdlvvsDgapdvsgvhdldeylqsqLllaalelalkllkpggtfvlkifrgeeteallkqlkkfFekvsvvkPkssrsesseeflvc 174 vd+ ++Dgap+v ++ d+y+qs+L l al+la++ l +gg f++k+fr+++ +al+ + ++F+kv+ +kP++sr+ s+e+f+vc tr|H2YL40|H2YL40_CIOSA 111 VDCFLHDGAPNVGANWLHDAYTQSELTLHALKLASEFLCKGGWFITKVFRSKDYQALMWVFGQLFHKVHATKPQASRNVSAEIFVVC 197 *************************************************************************************** PP EEE CS FtsJ 175 rgf 177 ++f tr|H2YL40|H2YL40_CIOSA 198 QNF 200 *98 PP >> tr|H2Z203|H2Z203_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 22.0 0.1 6.2e-08 9e-05 21 117 .. 215 325 .. 149 396 .. 0.75 Alignments for each domain: == domain 1 score: 22.0 bits; conditional E-value: 6.2e-08 TT-EEEEES-TT-HHHHHHTTT...-SEEEEEESS--...........---TT-EEEE--TTSSSHHH.HHHHHHHHSSEEEEEE-- CS FtsJ 21 pgktvvDlgaaPGgwsqvllqrlgakgkvvavDlqpm...........aplpgvttiqgditdpetie.kikealserkvdlvvsDg 95 p ++v+D +aaPG+ + l+ ++++gk++avD a+++++++i+ d+++ + + k+ +kv++++ D tr|H2Z203|H2Z203_CIOSA 215 PHTHVIDACAAPGNKTSHLAAIMNNTGKLFAVDRDRDrvkimerqlqkANVQNCEIINSDFMKLNPTDpKF------QKVECILVDP 295 5578*******************999********8877888887776556688888888887776665133......3699999999 PP --........---S-HHHHHHHHHHHHHHH CS FtsJ 96 ap........dvsgvhdldeylqsqLllaa 117 + d + ++d+ + + +L+l+ tr|H2Z203|H2Z203_CIOSA 296 SCsgsgivsrDLENQKDTNAEVLKRLALSK 325 973333333333333333333334555544 PP >> tr|H2YUK6|H2YUK6_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.6 0.0 2.2 3.2e+03 62 82 .. 162 185 .. 149 197 .. 0.76 2 ! 17.9 0.0 1.2e-06 0.0017 21 139 .. 208 352 .. 192 375 .. 0.55 Alignments for each domain: == domain 1 score: -2.6 bits; conditional E-value: 2.2 T-EEE...E--TTSSSHHHHHHHH CS FtsJ 62 gvtti...qgditdpetiekikea 82 gv+ + +gdit+ ++ k+k++ tr|H2YUK6|H2YUK6_CIOSA 162 GVRAYlyeKGDITEFDNPHKVKKL 185 555556779999999999998864 PP == domain 2 score: 17.9 bits; conditional E-value: 1.2e-06 TT-EEEEES-TT-HHHHHHTTT...-SEEEEEESS-----TT-EEEE--TTSSSHHHHHHHHHHH..........SSEEEEEE---- CS FtsJ 21 pgktvvDlgaaPGgwsqvllqrlgakgkvvavDlqpmaplpgvttiqgditdpetiekikealse..........rkvdlvvsDgap 97 pg++++D ++aPGg ++ q +g +g++va D+ + ++ ++++ i ++ e++ ++++ + d v+ D+ tr|H2YUK6|H2YUK6_CIOSA 208 PGDHILDMCSAPGGKLNIIMQAIGDSGRIVANDVDSKRLTRIKNVYKEYIPKQFSVEEFIKFVQFdgrewteietNAYDKVLVDVP- 293 7899******************************8874443333334444332222222222222334444444444444444442. PP ---S-HHHHHHHH.....................HHHHHHHHHHHHHHEEEEEEEEEEEE-ST CS FtsJ 98 dvsgvhdldeylq.....................sqLllaalelalkllkpggtfvlkifrge 139 +++ +h+l + + ++Ll++a+ ++p+g++v +++ + tr|H2YUK6|H2YUK6_CIOSA 294 CTNDRHSLYTSDRdadnifsnlrkreraqlphvqCELLKNAILA----CRPNGIVVYSTCTAS 352 22222222222222222222444444433322224555555444....567777777777644 PP >> tr|H2YGP5|H2YGP5_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 14.4 0.0 1.4e-05 0.02 21 80 .. 30 93 .. 20 156 .. 0.68 Alignments for each domain: == domain 1 score: 14.4 bits; conditional E-value: 1.4e-05 TT-EEEEES-TT-HHHHHHTTT...-SEEEEEESS--.....---TT-EEEE--T.TSSSHHHHHH CS FtsJ 21 pgktvvDlgaaPGgwsqvllqrlgakgkvvavDlqpm.....aplpgvttiqgdi.tdpetiekik 80 +g +vvDlg++ G + ++q +g++g v++vD+ + + + + ++ + +++++ + ++ tr|H2YGP5|H2YGP5_CIOSA 30 EGLKVVDLGCGSGRDCYAIAQLVGENGEVIGVDMTKEqldiaR--KYEEYHRVKFgFKESNVRFVE 93 6789********************9*********776455430..333333333333333333333 PP >> tr|H2YFA0|H2YFA0_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.1 0.0 6.8e-05 0.098 14 99 .. 138 227 .. 125 282 .. 0.80 Alignments for each domain: == domain 1 score: 12.1 bits; conditional E-value: 6.8e-05 HHH--S-TT-EEEEES-TT-HHHHHHTTT...-SEEEEEESS--.........---TT-EEEE--TTSSSHHHHHHHHHHHSSEEEE CS FtsJ 14 ekfklfkpgktvvDlgaaPGgwsqvllqrlgakgkvvavDlqpm.........aplpgvttiqgditdpetiekikealserkvdlv 91 e+ + +kpg +v+ lgaa G ++ +g++g v+av + +++ ++ i d ++p + +++ vd + tr|H2YFA0|H2YFA0_CIOSA 138 EAIH-IKPGSKVLYLGAASGTTVSHVSDIVGPEGLVYAVEFSHRsgrdlinvaKKRTNIIPIIEDARHPHKYRMLVG-----MVDTI 218 6677.89**********************************998788666554444666666999999999998885.....59*** PP EE---.--- CS FtsJ 92 vsDga.pdv 99 +D+a pd tr|H2YFA0|H2YFA0_CIOSA 219 FADVAqPDQ 227 ***994443 PP >> tr|H2YC22|H2YC22_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.5 0.0 0.00011 0.16 21 137 .. 141 288 .. 103 314 .. 0.63 Alignments for each domain: == domain 1 score: 11.5 bits; conditional E-value: 0.00011 TT-EEEEES-TT-HHHHHHTTT..........-SEEEEEESS--..........---.TT-EEEE--TTSSSHHHHHHHHHHH...S CS FtsJ 21 pgktvvDlgaaPGgwsqvllqrlga.......kgkvvavDlqpm..........apl.pgvttiqgditdpetiekikealse...r 86 ++v+D +aaPG+ + +++ l+ +g va D + +++ p +++ d + i +k+ ++e tr|H2YC22|H2YC22_CIOSA 141 SHHKVLDMCAAPGSKTAQIVELLHRdennaipDGICVANDKDNKrcymlvhqigRLHsPSTIIVNHDASNFPEIL-VKNDVGEeipL 226 4589***************99994443333334777888875554565554433333444444455555554443.44444445667 PP SEEEEEE----..---S-HHHHHHHH......HHHHHHHHHH...HHHHEEEEEEEEEEEE- CS FtsJ 87 kvdlvvsDgap..dvsgvhdldeylq......sqLllaalel...alkllkpggtfvlkifr 137 + d v++D+ d + ++++d ++ + L + + ++ +l+ll+ gg +v ++ tr|H2YC22|H2YC22_CIOSA 227 RYDRVLCDVPCsgDGTMRKNTDVWVKwnqanaIYLHRTQYRIllrGLELLGSGGQLVYSTCS 288 79999999753333334455555555555555555555555533355677788887766655 PP >> tr|H2Z8K9|H2Z8K9_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.0 0.3 0.00015 0.22 48 129 .. 48 148 .. 37 191 .. 0.73 Alignments for each domain: == domain 1 score: 11.0 bits; conditional E-value: 0.00015 EEEEEESS-----TT-EEEE--TTSSSHHH..HHHHHHHH.......................SSEEEEEE-------S-HHHHHHH CS FtsJ 48 kvvavDlqpmaplpgvttiqgditdpetie..kikealse.......................rkvdlvvsDgapdvsgvhdldeyl 109 k+ ++D+ +a t+q +i + + + + +s+ ++ + v+ D+ + v+ ++d ++++ tr|H2Z8K9|H2Z8K9_CIOSA 48 KISSIDVDRVARELDFVTLQENISTVAFCNvdA----VSDldplfvklfklaqftieyllhsqEYLQSVINDMESRVTTTNDEKAAV 130 566777777766666666777775555444222....2223345555666777777888888899********************** PP HHHHHHHHHHHHHHHEEEEE CS FtsJ 110 qsqLllaalelalkllkpgg 129 ++qL++a e++ lk++ tr|H2Z8K9|H2Z8K9_CIOSA 131 EAQLVEANAEIT--KLKQEN 148 ********9994..444444 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (177 nodes) Target sequences: 11592 (4231397 residues searched) Passed MSV filter: 340 (0.0293306); expected 231.8 (0.02) Passed bias filter: 280 (0.0241546); expected 231.8 (0.02) Passed Vit filter: 28 (0.00241546); expected 11.6 (0.001) Passed Fwd filter: 8 (0.000690131); expected 0.1 (1e-05) Initial search space (Z): 11592 [actual number of targets] Domain search space (domZ): 8 [number of targets reported over threshold] # CPU time: 0.10u 0.01s 00:00:00.11 Elapsed: 00:00:00.04 # Mc/sec: 18723.93 // [ok]