# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.1b2 (February 2015); http://hmmer.org/
# Copyright (C) 2015 Howard Hughes Medical Institute.
# Freely distributed under the GNU General Public License (GPLv3).
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: pfam_list/GCD14.hmm.txt
# target sequence database: proteomes/Amphimedon_queenslandica.fasta
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: GCD14 [M=247]
Accession: PF08704.9
Description: tRNA methyltransferase complex GCD14 subunit
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
5.5e-94 315.7 0.1 9.7e-94 314.9 0.1 1.4 1 tr|A0A1X7U326|A0A1X7U326_AMPQE tRNA (adenine(58)-N(1))-methyltr
2.9e-16 61.2 0.0 5.2e-16 60.3 0.0 1.4 1 tr|A0A1X7VAV2|A0A1X7VAV2_AMPQE Uncharacterized protein OS=Amphi
2.7e-05 25.2 0.0 4.1e-05 24.6 0.0 1.2 1 tr|A0A1X7UZ07|A0A1X7UZ07_AMPQE Uncharacterized protein OS=Amphi
0.0095 16.9 0.0 0.013 16.4 0.0 1.2 1 tr|A0A1X7V969|A0A1X7V969_AMPQE Uncharacterized protein OS=Amphi
------ inclusion threshold ------
0.043 14.7 0.0 0.043 14.7 0.0 2.9 4 tr|A0A1X7V4K8|A0A1X7V4K8_AMPQE Uncharacterized protein OS=Amphi
0.045 14.7 0.0 0.059 14.3 0.0 1.2 1 tr|A0A1X7VHW4|A0A1X7VHW4_AMPQE Uncharacterized protein OS=Amphi
0.045 14.7 0.0 0.067 14.1 0.0 1.2 1 tr|A0A1X7VEF1|A0A1X7VEF1_AMPQE Uncharacterized protein OS=Amphi
0.81 10.6 5.2 1 10.3 5.1 1.3 1 tr|A0A1X7UXL0|A0A1X7UXL0_AMPQE Uncharacterized protein OS=Amphi
1.4 9.8 5.6 1.6 9.6 5.6 1.1 1 tr|A0A1X7USH2|A0A1X7USH2_AMPQE Uncharacterized protein OS=Amphi
2.4 9.0 0.0 2.4 9.0 0.0 2.9 2 tr|A0A1X7SE98|A0A1X7SE98_AMPQE Uncharacterized protein OS=Amphi
2.5 9.0 0.0 2.5 9.0 0.0 2.9 1 tr|A0A1X7TAB4|A0A1X7TAB4_AMPQE Uncharacterized protein OS=Amphi
Domain annotation for each sequence (and alignments):
>> tr|A0A1X7U326|A0A1X7U326_AMPQE tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Amphimedon que
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 314.9 0.1 2.4e-97 9.7e-94 1 246 [. 64 296 .. 64 297 .. 0.92
Alignments for each domain:
== domain 1 score: 314.9 bits; conditional E-value: 2.4e-97
GCD14 1 gfvyvlaptPeLWtlaLphrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqrae 79
g+v vl+ptPeLWtl+Lphrtqily++Dis+i+m+Lel+pGs v+e GtGsgslshaiart+aP Ghlftfefhe+r +
tr|A0A1X7U326|A0A1X7U326_AMPQE 64 GWVIVLHPTPELWTLSLPHRTQILYSTDISMITMQLELRPGSTVCECGTGSGSLSHAIARTIAPDGHLFTFEFHEKRSQ 142
8****************************************************************************** PP
GCD14 80 kareefrehelsqlvtvthrDvckeGfdeevsgkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtcea 158
a++ef+ h+ls++vtv+ Dvc+eGf ++++ vDavfLDlP+Pweai +a++a+k+ ggr+CsfsPCieqvq+tce+
tr|A0A1X7U326|A0A1X7U326_AMPQE 143 IAKDEFEAHGLSHIVTVKCSDVCQEGF--GLEDRVDAVFLDLPNPWEAISSAKTAIKRTGGRICSFSPCIEQVQKTCEE 219
***************************..999*********************************************** PP
GCD14 159 LrelgfteietlevllrtyevrkskleeidlaekrleenerkriekakrrkaskdakvedkssksstevllkPvselkg 237
L++lgf++i ++e llr ++vr ++ +++ + +++++++ +k + as+++ + +++ g
tr|A0A1X7U326|A0A1X7U326_AMPQE 220 LNKLGFKDIGVMECLLRPFDVRPCVFNIPNMGSNVDHSTDKSKSTKLTQPIASSETIWTCA-----------QRRTIPG 287
*******************************998877776664444444444443333322...........236899* PP
GCD14 238 htgyLtfat 246
htg+Ltfat
tr|A0A1X7U326|A0A1X7U326_AMPQE 288 HTGFLTFAT 296
********8 PP
>> tr|A0A1X7VAV2|A0A1X7VAV2_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 60.3 0.0 1.3e-19 5.2e-16 3 210 .. 108 328 .. 106 364 .. 0.69
Alignments for each domain:
== domain 1 score: 60.3 bits; conditional E-value: 1.3e-19
GCD14 3 vyvlaptPeLWtlaLphrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraeka 81
v++ +p e + l +p + y +Di + L++ pGs+v+e+G Gsgsl+ ++r+va G++++fe ++ e+a
tr|A0A1X7VAV2|A0A1X7VAV2_AMPQE 108 VFIRRPSLEEYVLLMPRGPVVSYPKDIWAVLGLLDVGPGSRVLEAGSGSGSLTLHLSRAVADSGKVHSFELKDKHLETA 186
5666666777777788778888********************************************************* PP
GCD14 82 reefrehel..........sqlvtvthrDvckeGfdeev.sgkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCi 149
r + + + + ++ ++ e d+ + s++vD v LD+ +++a +++ ke++ + i
tr|A0A1X7VAV2|A0A1X7VAV2_AMPQE 187 RGNVSRWGRsrdlgggedfTRNIDFYNCS--VEECDKILgSETVDGVVLDMVDCRRVMKSALSVM-KENAIAVIYCTNI 262
*9986655433336663222233333333..333333333568**********************.5555556666667 PP
GCD14 150 eqvqrtc..eaLrelgf..teie.tlevllrtyevrkskleeidlaekrleenerkriekakrrka 210
q + +L++ + ie +levl r++ v+ + + +++ + + n+++ + +r++
tr|A0A1X7VAV2|A0A1X7VAV2_AMPQE 263 SQAIQLNyhLELHKNKLppHHIEsMLEVLHRHWLVQPASQKRKHVSQLYPKLNKNNDEYGHERSQD 328
76544330144555444225775389*********9877666666555555555554444444433 PP
>> tr|A0A1X7UZ07|A0A1X7UZ07_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=3 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 24.6 0.0 1e-08 4.1e-05 35 151 .. 79 196 .. 71 212 .. 0.72
Alignments for each domain:
== domain 1 score: 24.6 bits; conditional E-value: 1e-08
GCD14 35 kLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvc.keGfdeevsg 112
++++ pGs+v+ G+ sg+ + ++ v+P G ++++ef ++ + + + +++ ++ D+ + + + + g
tr|A0A1X7UZ07|A0A1X7UZ07_AMPQE 79 NIHIGPGSKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRD-LINVAKKR--TNIIPIIEDARhPHKY-RMLMG 153
67899**********************************6544433.33333333..2333334455514455.6788* PP
GCD14 113 kvDavfLDlPaPwea..ip.haakalkkeggrlCsf.sPCieq 151
vD++f D+ +P++a ++ +a + lk++g + s + Ci+
tr|A0A1X7UZ07|A0A1X7UZ07_AMPQE 154 MVDTIFADVAQPDQAriVGiNALHFLKNQGHFVISIkANCIDS 196
**************94332245556677777766652567765 PP
>> tr|A0A1X7V969|A0A1X7V969_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 16.4 0.0 3.3e-06 0.013 19 85 .. 8 74 .. 1 92 [. 0.86
Alignments for each domain:
== domain 1 score: 16.4 bits; conditional E-value: 3.3e-06
GCD14 19 hrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareef 85
hr + + D +i ++ +k+G v++ G G+g l+ +++ v+ G++ +++ ++r+e a+e
tr|A0A1X7V969|A0A1X7V969_AMPQE 8 HRHSSMQKRDGLKLIEEISVKEGDHVLDLGCGTGHLAVVLSKIVGEEGKVIAVDPNNERIELAKEVN 74
77777777787788999********************************************998755 PP
>> tr|A0A1X7V4K8|A0A1X7V4K8_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=3 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? -4.6 4.1 8.6 3.4e+04 216 216 .. 290 290 .. 222 389 .. 0.57
2 ? -3.7 2.8 4.7 1.8e+04 159 208 .. 336 385 .. 298 443 .. 0.65
3 ? -2.4 0.4 1.8 7.3e+03 205 218 .. 431 444 .. 379 484 .. 0.50
4 ? 14.7 0.0 1.1e-05 0.043 12 102 .. 755 845 .. 746 861 .. 0.76
Alignments for each domain:
== domain 1 score: -4.6 bits; conditional E-value: 8.6
GCD14 216 v 216
v
tr|A0A1X7V4K8|A0A1X7V4K8_AMPQE 290 V 290
1 PP
== domain 2 score: -3.7 bits; conditional E-value: 4.7
GCD14 159 Lrelgfteietlevllrtyevrkskleeidlaekrleenerkriekakrr 208
r++ e++ le ++ e k+ l++ ++ k+ ++ +ri ++k +
tr|A0A1X7V4K8|A0A1X7V4K8_AMPQE 336 ERDTLISELSELEAECKELESAKELLKTENEELKNDIAQKDDRIANMKLN 385
44455566666777777776666666666665544444444445444433 PP
== domain 3 score: -2.4 bits; conditional E-value: 1.8
GCD14 205 akrrkaskdakved 218
k ++as +++e
tr|A0A1X7V4K8|A0A1X7V4K8_AMPQE 431 LKAERASLTQEKER 444
22111111111111 PP
== domain 4 score: 14.7 bits; conditional E-value: 1.1e-05
GCD14 12 LWtlaLphrtqilytaDisliim.kLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehe 89
L +L +i+ + s i L pG+ v+++ + gs ++ ia +++ tG +++f+ ++r + + +r+ +
tr|A0A1X7V4K8|A0A1X7V4K8_AMPQE 755 LTNTSLYTSGKIILQDKSSCIASyVLSPPPGASVIDACAAPGSKTSHIAAAMKNTGSIHAFDRDKKRCDAMKALLRKYN 833
555556666667777666666541577789*************************************998777777777 PP
GCD14 90 lsqlvtvthrDvc 102
+ vtv++ D
tr|A0A1X7V4K8|A0A1X7V4K8_AMPQE 834 V-TCVTVSNMDFL 845
6.57888887743 PP
>> tr|A0A1X7VHW4|A0A1X7VHW4_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 14.3 0.0 1.5e-05 0.059 43 87 .. 31 75 .. 18 102 .. 0.86
Alignments for each domain:
== domain 1 score: 14.3 bits; conditional E-value: 1.5e-05
GCD14 43 vvvesGtGsgslshaiartvaPtGhlftfefheqraekareefre 87
+v++ G G+g ls+ i++ v+ G++ ++ e+r++ a++e +
tr|A0A1X7VHW4|A0A1X7VHW4_AMPQE 31 KVLDIGCGTGRLSKVISKRVGNGGKVIGVDPDEERIKIAINERKG 75
69999***********************************99775 PP
>> tr|A0A1X7VEF1|A0A1X7VEF1_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 14.1 0.0 1.7e-05 0.067 35 99 .. 26 89 .. 12 102 .. 0.83
Alignments for each domain:
== domain 1 score: 14.1 bits; conditional E-value: 1.7e-05
GCD14 35 kLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthr 99
+L ++ +v++ G G+g l++ +a v+P G + ++ e+r++ a+ee + h+ s+l+ v
tr|A0A1X7VEF1|A0A1X7VEF1_AMPQE 26 ELSPQEKDKVLDLGCGTGRLTQVLAERVGPEGLVVGIDPDEERIKVAIEE-KGHGRSNLIFVVAD 89
55666667899*************************************99.66777777666655 PP
>> tr|A0A1X7UXL0|A0A1X7UXL0_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 10.3 5.1 0.00025 1 147 213 .. 36 102 .. 12 110 .] 0.77
Alignments for each domain:
== domain 1 score: 10.3 bits; conditional E-value: 0.00025
GCD14 147 PCieqvqrtceaLrelgfteietlevllrtyevrkskleeidl.aekrleenerkriekakrrkaskd 213
Ci+ +++ ++ L+e + + ++ levl ++ ++r +++++ +++r + e ++ e +k+++ + +
tr|A0A1X7UXL0|A0A1X7UXL0_AMPQE 36 KCIQTMRNEIQGLTENNNKLVNELEVLHKRRQARLVRVRHQVHeGRQRNRQLELRANEIKKQIE-KAK 102
5************************************998655377766666665444444433.333 PP
>> tr|A0A1X7USH2|A0A1X7USH2_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 9.6 5.6 0.00041 1.6 170 233 .. 30 94 .. 11 102 .. 0.66
Alignments for each domain:
== domain 1 score: 9.6 bits; conditional E-value: 0.00041
GCD14 170 levllrtyevrkskleeidlaekrleenerkriekakrrka.skdakvedkssksstevllkPvs 233
+ l + ++ r+++l e + a++ + e ++++ ++++++k ++++k++ +++ ++e++ k ++
tr|A0A1X7USH2|A0A1X7USH2_AMPQE 30 VSSLKQPWNKRQRELAEKKAAKELERELKETARQEKEEKKRrTEENKKRREENAKKSEIVQKITN 94
3347899*********9999888887766665555544444155555555555557777776544 PP
>> tr|A0A1X7SE98|A0A1X7SE98_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? -2.2 3.5 1.6 6.2e+03 183 222 .. 18 57 .. 3 74 .. 0.38
2 ? 9.0 0.0 0.0006 2.4 41 70 .. 299 328 .. 279 334 .. 0.86
Alignments for each domain:
== domain 1 score: -2.2 bits; conditional E-value: 1.6
GCD14 183 kleeidlaekrleenerkriekakrrkaskdakvedkssk 222
+lee ++++ + +++ e+++r+ + +++ ++++ +
tr|A0A1X7SE98|A0A1X7SE98_AMPQE 18 ELEEERREKEQVRNRLQQQLEGRERQLEQAQQQGQERERQ 57
4444433333333333333333333322222222222222 PP
== domain 2 score: 9.0 bits; conditional E-value: 0.0006
GCD14 41 GsvvvesGtGsgslshaiartvaPtGhlft 70
G ++ s Gs+ l h i+r+v+P G l++
tr|A0A1X7SE98|A0A1X7SE98_AMPQE 299 GWRAKVSDYGSANLQHLIIRSVNPGGPLYA 328
556666889*******************96 PP
>> tr|A0A1X7TAB4|A0A1X7TAB4_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 9.0 0.0 0.00064 2.5 40 70 .. 406 436 .. 387 444 .. 0.82
Alignments for each domain:
== domain 1 score: 9.0 bits; conditional E-value: 0.00064
GCD14 40 pGsvvvesGtGsgslshaiartvaPtGhlft 70
G ++ s Gs+ l h i+r+v+P G l++
tr|A0A1X7TAB4|A0A1X7TAB4_AMPQE 406 VGWRAKVSDYGSANLQHLIIRSVNPGGPLYA 436
5777778899*******************96 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (247 nodes)
Target sequences: 43435 (14716620 residues searched)
Passed MSV filter: 2256 (0.0519397); expected 868.7 (0.02)
Passed bias filter: 1374 (0.0316335); expected 868.7 (0.02)
Passed Vit filter: 158 (0.00363762); expected 43.4 (0.001)
Passed Fwd filter: 33 (0.000759756); expected 0.4 (1e-05)
Initial search space (Z): 43435 [actual number of targets]
Domain search space (domZ): 11 [number of targets reported over threshold]
# CPU time: 0.55u 0.02s 00:00:00.57 Elapsed: 00:00:00.16
# Mc/sec: 22718.78
//
[ok]