# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/GCD14.hmm.txt # target sequence database: proteomes/Anolis_carolinensis.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: GCD14 [M=247] Accession: PF08704.9 Description: tRNA methyltransferase complex GCD14 subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-111 372.6 0.0 1.2e-111 372.3 0.0 1.0 1 tr|R4GBY7|R4GBY7_ANOCA tRNA (adenine(58)-N(1))-methyltransfe 1.2e-21 77.6 0.0 1.6e-21 77.1 0.0 1.1 1 tr|G1KIR9|G1KIR9_ANOCA tRNA (adenine(58)-N(1))-methyltransfe 3.9e-05 23.5 0.0 4.8e-05 23.2 0.0 1.2 1 tr|H9GKF5|H9GKF5_ANOCA Protein-L-isoaspartate O-methyltransf 6.3e-05 22.8 0.0 8.8e-05 22.3 0.0 1.2 1 tr|H9GNL4|H9GNL4_ANOCA Uncharacterized protein OS=Anolis car 9.9e-05 22.2 0.0 0.00014 21.6 0.0 1.2 1 tr|H9GBW3|H9GBW3_ANOCA Protein-L-isoaspartate (D-aspartate) ------ inclusion threshold ------ 0.016 14.9 0.0 0.055 13.2 0.0 1.7 2 tr|H9GPW8|H9GPW8_ANOCA Protein-L-isoaspartate (D-aspartate) 0.022 14.5 0.0 0.04 13.6 0.0 1.4 1 tr|G1KF57|G1KF57_ANOCA Protein-L-isoaspartate (D-aspartate) Domain annotation for each sequence (and alignments): >> tr|R4GBY7|R4GBY7_ANOCA tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Anolis carolinensis OX # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 372.3 0.0 4.6e-115 1.2e-111 1 247 [] 64 304 .. 64 304 .. 0.95 Alignments for each domain: == domain 1 score: 372.3 bits; conditional E-value: 4.6e-115 GCD14 1 gfvyvlaptPeLWtlaLphrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefre 87 g+vy+l+ptPeLWtl+Lphrtqily++Disli+m+LelkpGs v+esGtGsgslshai+rt+aPtGhl+t+efh+qraeka+eefre tr|R4GBY7|R4GBY7_ANOCA 64 GWVYILHPTPELWTLNLPHRTQILYSTDISLITMMLELKPGSIVCESGTGSGSLSHAIIRTIAPTGHLYTVEFHQQRAEKAIEEFRE 150 8************************************************************************************** PP GCD14 88 helsqlvtvthrDvckeGfdeevsgkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtceaLrelgfteietlevll 174 h++++lvtv+++Dvck+Gf +v++++DavfLD+P+Pweai+ha++alk+eggr+CsfsPCieqvqrtc aL+e+gftei+t e+ll tr|R4GBY7|R4GBY7_ANOCA 151 HKVDHLVTVKNQDVCKNGF--GVTDVADAVFLDIPSPWEAIGHAKSALKHEGGRICSFSPCIEQVQRTCLALEEHGFTEINTSEILL 235 *******************..99999************************************************************* PP GCD14 175 rtyevrkskleeidlaekrleenerkriekakrrkaskdakvedkssksstevllkPvselkghtgyLtfatk 247 r+y+vr+++l+ dl+++++++ + + ++r + s++++++ + ++ + + P++e++ghtgyLtfatk tr|R4GBY7|R4GBY7_ANOCA 236 RVYNVRTISLQIPDLGKAAEDNASAT---AFDRGSPSNQDAASPQAGT-VQFKSSVPLREMVGHTGYLTFATK 304 *****************999988555...6777777777777778887.4555678***************86 PP >> tr|G1KIR9|G1KIR9_ANOCA tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Anolis carolinensis OX # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 77.1 0.0 6.1e-25 1.6e-21 7 243 .. 159 410 .. 154 414 .. 0.77 Alignments for each domain: == domain 1 score: 77.1 bits; conditional E-value: 6.1e-25 GCD14 7 aptPeLWtlaLphrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefre....he 89 +pt e + l ++ i y +Dis++ +++ +G +v+e+G Gsg +s ++r+v+P+Gh+ ++e ++ + a+++++ + tr|G1KIR9|G1KIR9_ANOCA 159 RPTLEEFVLLMKRGPTIAYPKDISTMLLLMDISQGDKVLEAGSGSGGMSLFLSRAVGPQGHIISYEIRKDHHKLAKKNYQTwcdaWR 245 5666777777777789************************************************************99986333323 PP GCD14 90 l......sqlvtvthrDvckeGfdeevsgkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtceaLrelg...ftei 167 + ++v + D+ + d + + Da+ LD+ P +a+p ++ l k+gg+ + i qv ea+r + f+e tr|G1KIR9|G1KIR9_ANOCA 246 IghtvewPDNVDFINADILTAAEDLKT-ITFDAIALDMLLPQNALPTVIPNL-KQGGVCTVYLANITQVVDLLEAIRTRKlnlFCE- 329 344435456888888888877653332.479*********************.8888888999***********999876333455. PP GCD14 168 etlevllrtyevrkskleeidlaekrleenerk.....riekakrrkaskdakvedkssksstevllkPvselkghtgyLt 243 + +ev r + v ++ ++++++++r+ + + + r e+ + + ++++ + s+ ++ +++P +ght++L+ tr|G1KIR9|G1KIR9_ANOCA 330 RIVEVTHRDWLVLPNTWRNSSIFQSRETKPDFDdeldgRDENNDPSAEEREHDEAFVSNGTKPPYIARPYPWQTGHTAFLI 410 899*******9988888888887777665544400000222222223344444444455558899**************96 PP >> tr|H9GKF5|H9GKF5_ANOCA Protein-L-isoaspartate O-methyltransferase OS=Anolis carolinensis OX=28377 GN=LOC100554054 PE # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 23.2 0.0 1.8e-08 4.8e-05 36 90 .. 74 128 .. 55 180 .. 0.79 Alignments for each domain: == domain 1 score: 23.2 bits; conditional E-value: 1.8e-08 GCD14 36 LelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehel 90 +l +G+++++ G Gsg l+ +ar v+PtG+ +e+ e+ +++++++ re++ tr|H9GKF5|H9GKF5_ANOCA 74 EQLVEGAKALDVGSGSGYLTACFARMVGPTGKAVGIEHIEELVHESIKNVREDDP 128 46899*******************************************9999764 PP >> tr|H9GNL4|H9GNL4_ANOCA Uncharacterized protein OS=Anolis carolinensis OX=28377 GN=fbl PE=3 SV=2 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 22.3 0.0 3.3e-08 8.8e-05 34 139 .. 143 248 .. 135 279 .. 0.72 Alignments for each domain: == domain 1 score: 22.3 bits; conditional E-value: 3.3e-08 GCD14 34 mkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvc.keGfdeevsgkvDavfL 119 ++++kpG++v+ G+ sg+ + ++ v+P G ++++ef ++ + + + +++ ++++ v + D+ + + + + g vD +f tr|H9GNL4|H9GNL4_ANOCA 143 DQIHIKPGAKVLYLGAASGTTVSHVSDIVGPEGLVYAVEFSHRSGRD-LINVAKKR-TNIIPVIE-DARhPHKY-RMLIGMVDVIFA 225 5789************************************7544433.33443333.34455544.54415666.5566******** PP GCD14 120 DlPaPwea..i.phaakalkkeg 139 D+ +P+++ + +a + lk+ g tr|H9GNL4|H9GNL4_ANOCA 226 DVAQPDQSriVaLNAHNFLKNGG 248 *****986322124445554444 PP >> tr|H9GBW3|H9GBW3_ANOCA Protein-L-isoaspartate (D-aspartate) O-methyltransferase OS=Anolis carolinensis OX=28377 GN=P # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 21.6 0.0 5.4e-08 0.00014 37 88 .. 75 126 .. 63 147 .. 0.90 Alignments for each domain: == domain 1 score: 21.6 bits; conditional E-value: 5.4e-08 GCD14 37 elkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefreh 88 +l++G+++++ G Gsg l+ +ar v+P+G++ +++ ++ ++ ++++ r++ tr|H9GBW3|H9GBW3_ANOCA 75 QLHEGAKALDVGSGSGILTACFARMVGPKGKVVGIDHIKELVDDSINNVRKD 126 6899*********************************999999999998875 PP >> tr|H9GPW8|H9GPW8_ANOCA Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1 OS=Anolis caroli # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 13.2 0.0 2.1e-05 0.055 30 85 .. 70 125 .. 60 134 .. 0.92 2 ? -1.6 0.0 0.68 1.8e+03 206 223 .. 305 322 .. 280 355 .. 0.58 Alignments for each domain: == domain 1 score: 13.2 bits; conditional E-value: 2.1e-05 GCD14 30 sliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareef 85 s ++ L+l+pG ++ G G+g ls+ + ++P G + +e h + +e a+e + tr|H9GPW8|H9GPW8_ANOCA 70 SEVMEALKLQPGLSFLNLGSGTGYLSTMVGLILGPFGINHGIELHPDVVEYAKERL 125 568899****************************************9999998765 PP == domain 2 score: -1.6 bits; conditional E-value: 0.68 GCD14 206 krrkaskdakvedkssks 223 + +k+++d+k ed+++++ tr|H9GPW8|H9GPW8_ANOCA 305 EDEKMEEDNKEEDEKEHT 322 334444444444444443 PP >> tr|G1KF57|G1KF57_ANOCA Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 2 OS=Anolis caroli # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 13.6 0.0 1.5e-05 0.04 30 84 .. 70 124 .. 57 132 .. 0.92 Alignments for each domain: == domain 1 score: 13.6 bits; conditional E-value: 1.5e-05 GCD14 30 sliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekaree 84 s ++ L+l+pG ++ G G+g ls+ + ++P G + +e h + +e a+++ tr|G1KF57|G1KF57_ANOCA 70 SEVMEALDLQPGLSFLNLGSGTGYLSSMVGLILGPFGINHGVELHSDVIEYAKQK 124 568899*****************************************99999875 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (247 nodes) Target sequences: 18528 (9796552 residues searched) Passed MSV filter: 1146 (0.0618523); expected 370.6 (0.02) Passed bias filter: 744 (0.0401554); expected 370.6 (0.02) Passed Vit filter: 74 (0.00399396); expected 18.5 (0.001) Passed Fwd filter: 14 (0.000755613); expected 0.2 (1e-05) Initial search space (Z): 18528 [actual number of targets] Domain search space (domZ): 7 [number of targets reported over threshold] # CPU time: 0.33u 0.01s 00:00:00.34 Elapsed: 00:00:00.10 # Mc/sec: 24197.48 // [ok]