# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/GCD14.hmm.txt # target sequence database: proteomes/Ciona_savignyi.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: GCD14 [M=247] Accession: PF08704.9 Description: tRNA methyltransferase complex GCD14 subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.5e-89 298.1 0.0 4.4e-89 297.8 0.0 1.1 1 tr|H2Z0U5|H2Z0U5_CIOSA tRNA (adenine(58)-N(1))-methyltransfe 1.1e-05 24.7 0.1 1.6e-05 24.1 0.1 1.2 1 tr|H2YFA0|H2YFA0_CIOSA Uncharacterized protein OS=Ciona savi ------ inclusion threshold ------ 1 8.4 5.7 14 4.6 5.0 2.4 2 tr|H2YBX1|H2YBX1_CIOSA Uncharacterized protein OS=Ciona savi 4.4 6.3 11.5 1.1 8.3 4.2 2.0 2 tr|H2Y9H9|H2Y9H9_CIOSA Uncharacterized protein OS=Ciona savi Domain annotation for each sequence (and alignments): >> tr|H2Z0U5|H2Z0U5_CIOSA tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Ciona savignyi OX=5151 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 297.8 0.0 1.5e-92 4.4e-89 1 194 [. 62 255 .. 62 268 .. 0.95 Alignments for each domain: == domain 1 score: 297.8 bits; conditional E-value: 1.5e-92 GCD14 1 gfvyvlaptPeLWtlaLphrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefre 87 g+vyvl+ptPeLWt++Lphrtqilyt Dis+i ++L+lkpGsvv+esGtGsgslshai+rt+aP G l+t+efhe raekar ef+e tr|H2Z0U5|H2Z0U5_CIOSA 62 GWVYVLQPTPELWTITLPHRTQILYTVDISMILFQLDLKPGSVVCESGTGSGSLSHAIIRTIAPSGYLHTVEFHEGRAEKARLEFKE 148 8************************************************************************************** PP GCD14 88 helsqlvtvthrDvckeGfdeevsgkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtceaLre..lgfteietlev 172 h+ls++vtv+hrDv k+Gf v++++Da+fLD+P+P +ai a++alkk+ggrlCsfsPCieqvqrtc aLr+ +gf +i+ +e+ tr|H2Z0U5|H2Z0U5_CIOSA 149 HGLSNYVTVYHRDVLKDGF--PVTSVADAIFLDIPSPENAISTASNALKKSGGRLCSFSPCIEQVQRTCSALRQkgSGFRDIQIFEL 233 *******************..88999**********************************************983357********* PP GCD14 173 llrtyevrkskleeidlaekrl 194 r +v+++ ++++dl+ + + tr|H2Z0U5|H2Z0U5_CIOSA 234 VRRSSNVKRIFVPMADLGLADE 255 ***************9977654 PP >> tr|H2YFA0|H2YFA0_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 24.1 0.1 5.5e-09 1.6e-05 35 150 .. 139 255 .. 133 272 .. 0.70 Alignments for each domain: == domain 1 score: 24.1 bits; conditional E-value: 5.5e-09 GCD14 35 kLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvc.keGfdeevsgkvDavfLD 120 +++kpGs+v+ G+ sg+ + ++ v+P G ++++ef ++ + + + +++ +++ + D+ + + + + g vD++f D tr|H2YFA0|H2YFA0_CIOSA 139 AIHIKPGSKVLYLGAASGTTVSHVSDIVGPEGLVYAVEFSHRSGRD-LINVAKKRT-NIIPI-IEDARhPHKY-RMLVGMVDTIFAD 221 5789***********************************7544433.333333332.33333.4455524555.5677********* PP GCD14 121 lPaPwea..ip.haakalkkeggrlCsf.sPCie 150 + +P++ + +a + lk+ g + s + Ci+ tr|H2YFA0|H2YFA0_CIOSA 222 VAQPDQTriVAlNAHHFLKNAGHFVISIkASCID 255 ****986322214444556666655555245776 PP >> tr|H2YBX1|H2YBX1_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 4.6 5.0 0.005 14 138 227 .. 12 101 .. 3 123 .. 0.67 2 ? 1.4 0.0 0.046 1.3e+02 49 97 .. 158 206 .. 154 217 .. 0.85 Alignments for each domain: == domain 1 score: 4.6 bits; conditional E-value: 0.005 GCD14 138 eggrlCsfsPCieqvqrtceaLrelgfteietlevllrtyevrkskleeidl.aekrleenerkriekakrrkaskdakvedkssks 223 + ++ s s ++ +q+ ++ L ++ +i + ++ + ev ++ ++e+++ +++++en+ +++ ++++ s++ k + ++++ tr|H2YBX1|H2YBX1_CIOSA 12 DRTKATSNSLIVDALQQVIKSLLTKDSDDIVKFRAQTEKLEVLQKAVKEMQMkVKAHQDENHLLKANVNEEKRRSDQYKHKLQKAQ- 97 55667788889999*************************************94455555555555544444444444455555544. PP GCD14 224 stev 227 ++ + tr|H2YBX1|H2YBX1_CIOSA 98 EALI 101 3333 PP == domain 2 score: 1.4 bits; conditional E-value: 0.046 GCD14 49 tGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvt 97 t s s h++++ + + +l f +r +kar+e +++++++ t++ tr|H2YBX1|H2YBX1_CIOSA 158 TASISEDHSFTKPLPKRKRLSKTSFRSDRLKKARNERMSQDVKEIETIK 206 6677778999999999999999**************9999998877776 PP >> tr|H2Y9H9|H2Y9H9_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 8.3 4.2 0.00037 1.1 164 229 .. 6 71 .. 1 87 [. 0.55 2 ? 3.0 5.4 0.015 44 157 215 .. 57 117 .. 50 134 .. 0.60 Alignments for each domain: == domain 1 score: 8.3 bits; conditional E-value: 0.00037 GCD14 164 fteietlevllrtyevrkskleeidl.aekrleenerkriekakrrkaskdakvedkssksstevll 229 ++++t+e+ll+++++ k++l+ +k++ + ++kr + ak++ a +++ ++++ + + e++ tr|H2Y9H9|H2Y9H9_CIOSA 6 GQDLSTIEILLQKHKTEKKSLQAEIQaIKKAVPKGDKKREKIAKQKIADLENALKETHKQ-ELETVE 71 589*************99999865551444444444444444444444333333333222.223332 PP == domain 2 score: 3.0 bits; conditional E-value: 0.015 GCD14 157 eaLrelgfteietlevllrtyevrkskleeidlaekrleenerkr..iekakrrkaskdak 215 +aL+e++ +e+et+e ++t ++ s+ + + +e+ e+ ++++ +++++++ka+k ++ tr|H2Y9H9|H2Y9H9_CIOSA 57 NALKETHKQELETVEKSQKTDAIDSSTTVLDNNGEEPSETRQKSKgkAKRKQEKKAMKAKE 117 6788999999999999888887777776666666555554433320022222233333333 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (247 nodes) Target sequences: 11592 (4231397 residues searched) Passed MSV filter: 678 (0.0584886); expected 231.8 (0.02) Passed bias filter: 402 (0.0346791); expected 231.8 (0.02) Passed Vit filter: 32 (0.00276052); expected 11.6 (0.001) Passed Fwd filter: 5 (0.000431332); expected 0.1 (1e-05) Initial search space (Z): 11592 [actual number of targets] Domain search space (domZ): 4 [number of targets reported over threshold] # CPU time: 0.13u 0.01s 00:00:00.14 Elapsed: 00:00:00.04 # Mc/sec: 26128.88 // [ok]