# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/GCD14.hmm.txt # target sequence database: proteomes/Monodelphis_domestica.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: GCD14 [M=247] Accession: PF08704.9 Description: tRNA methyltransferase complex GCD14 subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6.4e-104 347.2 0.5 1.9e-99 332.6 0.2 2.1 2 tr|F7F8B1|F7F8B1_MONDO tRNA (adenine(58)-N(1))-methyltransfe 1.4e-15 57.9 0.0 1.9e-15 57.5 0.0 1.2 1 tr|K7E169|K7E169_MONDO Uncharacterized protein OS=Monodelphi 9.4e-05 22.5 0.0 0.00013 21.9 0.0 1.2 1 tr|F6YAE8|F6YAE8_MONDO Fibrillarin OS=Monodelphis domestica 0.0034 17.4 0.0 0.0047 16.9 0.0 1.1 1 tr|H9H7D7|H9H7D7_MONDO Uncharacterized protein OS=Monodelphi 0.0043 17.0 0.0 0.0056 16.6 0.0 1.2 1 tr|F7FVJ3|F7FVJ3_MONDO Protein-L-isoaspartate O-methyltransf ------ inclusion threshold ------ 0.017 15.1 0.0 0.038 13.9 0.0 1.5 1 tr|F6VKL8|F6VKL8_MONDO Protein-L-isoaspartate (D-aspartate) 0.046 13.6 0.1 0.1 12.5 0.0 1.5 2 tr|F6QTB4|F6QTB4_MONDO Protein-L-isoaspartate (D-aspartate) Domain annotation for each sequence (and alignments): >> tr|F7F8B1|F7F8B1_MONDO tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Monodelphis domestica # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 332.6 0.2 6.2e-103 1.9e-99 1 189 [. 192 378 .. 192 383 .. 0.98 2 ! 12.9 0.0 2.5e-05 0.075 228 246 .. 395 413 .. 388 414 .. 0.85 Alignments for each domain: == domain 1 score: 332.6 bits; conditional E-value: 6.2e-103 GCD14 1 gfvyvlaptPeLWtlaLphrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefre 87 g+vyvl+ptPeLWtl+Lphrtqily++Dis+i+m+LelkpGs v+esGtGsgslshai+rtvaPtGhl+t+efh+qra+ka eefre tr|F7F8B1|F7F8B1_MONDO 192 GWVYVLHPTPELWTLNLPHRTQILYSTDISIITMMLELKPGSIVCESGTGSGSLSHAIIRTVAPTGHLHTVEFHQQRAQKAEEEFRE 278 8************************************************************************************** PP GCD14 88 helsqlvtvthrDvckeGfdeevsgkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtceaLrelgfteietlevll 174 h+++ +vtv+++Dvck+Gf +vs+++DavfLD+P+Pweai+ha++alk eggrlCsfsPCieqvqrtc+ L+e+gf+e++tl +ll tr|F7F8B1|F7F8B1_MONDO 279 HRVDRWVTVRNQDVCKNGF--GVSHVADAVFLDIPSPWEAIGHAKDALKVEGGRLCSFSPCIEQVQRTCHDLTEHGFIEVTTLKILL 363 *******************..9***************************************************************** PP GCD14 175 rtyevrkskleeidl 189 r+y+vr+s+l++ l tr|F7F8B1|F7F8B1_MONDO 364 RKYNVRTSHLPMQHL 378 ********9988766 PP == domain 2 score: 12.9 bits; conditional E-value: 2.5e-05 GCD14 228 llkPvselkghtgyLtfat 246 ++ P++e++ghtgyLtfa tr|F7F8B1|F7F8B1_MONDO 395 IAVPLKETVGHTGYLTFAS 413 678**************97 PP >> tr|K7E169|K7E169_MONDO Uncharacterized protein OS=Monodelphis domestica OX=13616 GN=TRMT61B PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 57.5 0.0 6.2e-19 1.9e-15 18 243 .. 251 497 .. 234 501 .. 0.75 Alignments for each domain: == domain 1 score: 57.5 bits; conditional E-value: 6.2e-19 GCD14 18 phrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehel..........sqlv 94 + i y +D+ ++ m++++k+G v+++ G Gsg +s ++++v+ +G++ +fe ++ ++a+++++ + ++v tr|K7E169|K7E169_MONDO 251 KRGPTISYPKDMNMMLMMMNIKQGDVILDVGSGSGGMSLFLSQAVGVRGQVKSFEIRKDHHNQAKKNYKCWRDawkishveewPNNV 337 4445799*************************************************9999999999987432222235545445689 PP GCD14 95 tvthrDvckeGfdeevsg.kvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtceaLr..elgfteietlevllrtye 178 h+D+ + ++++ D v LD+ +P a+p + l k+gg+ + i qv + e +r el + +evllr + tr|K7E169|K7E169_MONDO 338 DFIHKDILEAAE--DIESiIFDGVALDMLNPQIALPIVYPNL-KQGGVCAVYLANITQVIELLEGIRicELSLSCEQIIEVLLRDWL 421 9999**998766..5544167*********************.78888888999999998888766522455555689********* PP GCD14 179 vrkskleeidlaekrleenerk.riekakrrkaskdakvedkssks..........stevllkPv..selkghtgyLt 243 v k + la++ + +++ + + + ++++++++++++++++ s +++P+ + ht++L+ tr|K7E169|K7E169_MONDO 422 VCPAKRKDGTLAQRVQPKVSVDlKLHSQREIETEESENIQEND--YgeslpgfpysSIPYIARPFhyQANYNHTAFLV 497 *999999999988877777655133333444444444444444..333588888866777888875556678999986 PP >> tr|F6YAE8|F6YAE8_MONDO Fibrillarin OS=Monodelphis domestica OX=13616 GN=FBL PE=3 SV=2 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 21.9 0.0 4.4e-08 0.00013 35 141 .. 153 259 .. 144 291 .. 0.71 Alignments for each domain: == domain 1 score: 21.9 bits; conditional E-value: 4.4e-08 GCD14 35 kLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvc.keGfdeevsgkvDavfLD 120 +++++pG++v+ G+ sg+ + ++ v+P G ++++ef ++ + + + +++ ++++ v + D+ + + + + g vD +f D tr|F6YAE8|F6YAE8_MONDO 153 QIHIRPGAKVLYLGAASGTTVSHVSDIVGPEGLVYAVEFSHRSGRD-LINVAKKR-TNIIPVIE-DARhPHKY-RMLIGMVDVIFAD 235 789************************************7544433.33443333.34455544.54415666.5566********* PP GCD14 121 lPaPwea..i.phaakalkkeggr 141 + +P+++ + +a + l++ g tr|F6YAE8|F6YAE8_MONDO 236 VAQPDQSriVaLNAHNFLRNGGHF 259 ****98632212445555444444 PP >> tr|H9H7D7|H9H7D7_MONDO Uncharacterized protein OS=Monodelphis domestica OX=13616 PE=4 SV=2 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.9 0.0 1.5e-06 0.0047 41 120 .. 105 187 .. 86 197 .. 0.80 Alignments for each domain: == domain 1 score: 16.9 bits; conditional E-value: 1.5e-06 GCD14 41 GsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtv...thrDvckeGf.deevsgkvDavfLD 120 v +e Gt g + +ar + P +lft+ef e+ a+ a + + +l++ + + +++++ + ++e+ ++vD vfLD tr|H9H7D7|H9H7D7_MONDO 105 PRVLLELGTYCGYSAIRMARLLPPGARLFTIEFNESFASIASQMIQLAGLQDKISIlkgSTQEIIPQLKkKHEI-STVDLVFLD 187 457899*******9************************************987777232467777555436777.89999**99 PP >> tr|F7FVJ3|F7FVJ3_MONDO Protein-L-isoaspartate O-methyltransferase OS=Monodelphis domestica OX=13616 GN=PCMT1 PE=3 SV # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.6 0.0 1.9e-06 0.0056 37 89 .. 141 193 .. 130 246 .. 0.83 Alignments for each domain: == domain 1 score: 16.6 bits; conditional E-value: 1.9e-06 GCD14 37 elkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehe 89 +l+ G+++++ G Gsg l+ +ar v+ tG++ +++ ++ ++ ++ + ++++ tr|F7FVJ3|F7FVJ3_MONDO 141 QLHDGAKALDVGSGSGILTACFARMVGATGKVIGIDHIKELVDDSISNVKKDD 193 5789*********************************9999999999888765 PP >> tr|F6VKL8|F6VKL8_MONDO Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 2 OS=Monodelphis d # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 13.9 0.0 1.3e-05 0.038 30 84 .. 70 124 .. 57 132 .. 0.92 Alignments for each domain: == domain 1 score: 13.9 bits; conditional E-value: 1.3e-05 GCD14 30 sliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekaree 84 s ++ L+l+pG ++ G G+g ls+ + ++P G + +e h + +e a+++ tr|F6VKL8|F6VKL8_MONDO 70 SEVMEALDLQPGLSFLNLGSGTGYLSSMVGLILGPFGVNHGVELHSDVIEYAKQK 124 568899*****************************************99999875 PP >> tr|F6QTB4|F6QTB4_MONDO Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1 OS=Monodelphis d # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.5 0.0 3.4e-05 0.1 30 86 .. 70 126 .. 60 141 .. 0.90 2 ? -3.7 0.0 3 9.2e+03 208 222 .. 306 320 .. 294 347 .. 0.57 Alignments for each domain: == domain 1 score: 12.5 bits; conditional E-value: 3.4e-05 GCD14 30 sliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefr 86 s ++ L+l+pG ++ G G+g ls+ + +P G + +e h + +e a+e+++ tr|F6QTB4|F6QTB4_MONDO 70 SEVMEALKLQPGLSFLNLGSGTGYLSTMTSLVRSPFGINHGIELHSDVVEYAKEKLE 126 568899***********99999999999998889**************999998765 PP == domain 2 score: -3.7 bits; conditional E-value: 3 GCD14 208 rkaskdakvedkssk 222 +k+++d+k e+++++ tr|F6QTB4|F6QTB4_MONDO 306 EKMEEDHKEEEEKDQ 320 333333333333333 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (247 nodes) Target sequences: 21272 (11266248 residues searched) Passed MSV filter: 1397 (0.0656732); expected 425.4 (0.02) Passed bias filter: 935 (0.0439545); expected 425.4 (0.02) Passed Vit filter: 119 (0.00559421); expected 21.3 (0.001) Passed Fwd filter: 53 (0.00249154); expected 0.2 (1e-05) Initial search space (Z): 21272 [actual number of targets] Domain search space (domZ): 7 [number of targets reported over threshold] # CPU time: 0.51u 0.02s 00:00:00.53 Elapsed: 00:00:00.21 # Mc/sec: 13251.25 // [ok]