# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/GCD14.hmm.txt # target sequence database: proteomes/Nematostella_vectensis.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: GCD14 [M=247] Accession: PF08704.9 Description: tRNA methyltransferase complex GCD14 subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.3e-90 301.8 0.0 7.3e-90 301.4 0.0 1.1 1 tr|A7RHG9|A7RHG9_NEMVE tRNA (adenine(58)-N(1))-methyltransfe 7.4e-07 29.5 0.1 9.9e-07 29.1 0.1 1.1 1 tr|A7S758|A7S758_NEMVE Predicted protein OS=Nematostella vec 8.4e-05 22.8 0.0 0.00012 22.3 0.0 1.1 1 tr|A7S8G2|A7S8G2_NEMVE Predicted protein OS=Nematostella vec 0.00021 21.5 0.0 0.00027 21.1 0.0 1.2 1 tr|A7RWP0|A7RWP0_NEMVE Predicted protein OS=Nematostella vec 0.0031 17.7 11.4 0.35 11.0 2.0 2.8 2 tr|A7SRL0|A7SRL0_NEMVE Predicted protein OS=Nematostella vec 0.0049 17.0 0.0 0.0079 16.3 0.0 1.4 1 tr|A7SXL3|A7SXL3_NEMVE Protein-L-isoaspartate O-methyltransf 0.0062 16.7 0.0 0.0086 16.2 0.0 1.3 1 tr|A7SY89|A7SY89_NEMVE Predicted protein (Fragment) OS=Nemat 0.008 16.3 0.0 0.031 14.4 0.0 1.9 2 tr|A7RT21|A7RT21_NEMVE Predicted protein OS=Nematostella vec ------ inclusion threshold ------ 0.39 10.8 2.0 0.55 10.3 2.0 1.2 1 tr|A7SSV4|A7SSV4_NEMVE Predicted protein OS=Nematostella vec Domain annotation for each sequence (and alignments): >> tr|A7RHG9|A7RHG9_NEMVE tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Nematostella vectensis # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 301.4 0.0 2.7e-93 7.3e-90 1 192 [. 61 250 .. 61 255 .. 0.97 Alignments for each domain: == domain 1 score: 301.4 bits; conditional E-value: 2.7e-93 GCD14 1 gfvyvlaptPeLWtlaLphrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefre 87 g++++l+ptPeLWt++Lphrtqily++Dis++ m+L++kpGsvv+esGtGs+s+sha++r++aPtGhl+t+efhe+r ++ar+ef + tr|A7RHG9|A7RHG9_NEMVE 61 GYLFLLHPTPELWTINLPHRTQILYSTDISMVLMQLDIKPGSVVMESGTGSASMSHALIRSIAPTGHLYTYEFHEERSKQARKEFID 147 799************************************************************************************ PP GCD14 88 helsqlvtvthrDvckeGf.deevsgkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtceaLrelgfteietlevl 173 h++++lvtvthr+v +Gf +e++ +DavfLDlP+Pw+ai+ +kalk+eggrlCsfsPCieqvq+tc ++r+ gf +i+++e l tr|A7RHG9|A7RHG9_NEMVE 148 HNVDHLVTVTHRNVTADGFlEENI---ADAVFLDLPSPWDAIKFSKKALKREGGRLCSFSPCIEQVQKTCGVMRDNGFRDIQVMECL 231 *******************55555...************************************************************ PP GCD14 174 lrtyevrkskleeidlaek 192 l+t++v+++++++ d +ek tr|A7RHG9|A7RHG9_NEMVE 232 LKTFDVKTVSYQMPDFGEK 250 *************998765 PP >> tr|A7S758|A7S758_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g167288 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 29.1 0.1 3.6e-10 9.9e-07 31 121 .. 9 100 .. 4 122 .. 0.90 Alignments for each domain: == domain 1 score: 29.1 bits; conditional E-value: 3.6e-10 GCD14 31 liimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvckeGf.deevsgkvDa 116 ++ m+ ++ + +v++ Gt +g + a+a + P G++ t+ef ++ a++a + fr+ ++ + +++ D+ + ++k D tr|A7S758|A7S758_NEMVE 9 FLKMMAQIANAKRVLDVGTFTGMSAMAFAEGIPPDGQVVTIEFDQTIASTADKLFRDSAQAHKLALKVGDAVDVMTdLKSAQEKFDI 95 6789999***************999*******************************************999866553577889**** PP GCD14 117 vfLDl 121 +fLD tr|A7S758|A7S758_NEMVE 96 IFLDA 100 ***96 PP >> tr|A7S8G2|A7S8G2_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g186929 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 22.3 0.0 4.4e-08 0.00012 35 151 .. 132 249 .. 124 261 .. 0.69 Alignments for each domain: == domain 1 score: 22.3 bits; conditional E-value: 4.4e-08 GCD14 35 kLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvc.keGfdeevsgkvDavfLD 120 k+++ pGs+v+ G+ sg+ + ++ v+P G ++++ef ++ + + + +++ +++v + D+ + + + + g vD++f D tr|A7S8G2|A7S8G2_NEMVE 132 KIHMPPGSKVLYLGAASGTTVSHVSDIVGPEGLVYAVEFSHRSGRDLI-NVAKKR-TNIVPI-IEDARhPHKY-RMLVGMVDCIFAD 214 67889***************9******************654443333.333332.233333.3455514555.5677********* PP GCD14 121 lPaPwea...iphaakalkkeggrlCsf.sPCieq 151 + +P++ +a + lk+ g + s + Ci+ tr|A7S8G2|A7S8G2_NEMVE 215 VAQPDQTrivAINAHNFLKNGGHFVISIkANCIDS 249 ****9863212355666766555555542567765 PP >> tr|A7RWP0|A7RWP0_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g203274 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 21.1 0.0 1e-07 0.00027 34 94 .. 72 132 .. 62 184 .. 0.78 Alignments for each domain: == domain 1 score: 21.1 bits; conditional E-value: 1e-07 GCD14 34 mkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlv 94 + +lk+G+ +++ G Gsg l+ +a v+ +G+++ +++ +q +++a+++ ++ + + l tr|A7RWP0|A7RWP0_NEMVE 72 LEDQLKEGATALDVGSGSGYLTACMAYMVGEKGKVYGIDHIDQLVTEAKNNIKKGNPELLS 132 455799********************************************99987654444 PP >> tr|A7SRL0|A7SRL0_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g216349 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 11.0 2.0 0.00013 0.35 151 220 .. 3 71 .. 1 82 [. 0.65 2 ! 10.4 2.7 0.00018 0.5 163 215 .. 85 137 .. 75 148 .] 0.52 Alignments for each domain: == domain 1 score: 11.0 bits; conditional E-value: 0.00013 GCD14 151 qvqrtceaLrelgfteietlevllrtyevrkskleeidlaekrleenerkriekakrrkaskdakvedks 220 qv +++r+ g e+++ ++++r+ ++r++ ++++ +++ ++ +++ + ++++ ++++ + +d+ tr|A7SRL0|A7SRL0_NEMVE 3 QVDIRQNNMRQVGMREVDMSQIDIRQVDTREVGMRQVGMSQVHMRQVDMR-QVGMRQVDMKEADMRQDNM 71 55555667788888888888888888888888888888877777777544.4455555554444444433 PP == domain 2 score: 10.4 bits; conditional E-value: 0.00018 GCD14 163 gfteietlevllrtyevrkskleeidlaekrleenerkriekakrrkaskdak 215 + ++++ ev +r+ ++r+++++e+d+ ++++ e++ ++++ +k + d k tr|A7SRL0|A7SRL0_NEMVE 85 DIRQVDMGEVGIRQVDMRQVDMKEVDMRQDNMREVDIRQVDMRKVDMRQVDMK 137 44555666666666666666666666666666666555444433322222222 PP >> tr|A7SXL3|A7SXL3_NEMVE Protein-L-isoaspartate O-methyltransferase OS=Nematostella vectensis OX=45351 GN=v1g136839 PE # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.3 0.0 2.9e-06 0.0079 36 85 .. 74 123 .. 64 166 .. 0.87 Alignments for each domain: == domain 1 score: 16.3 bits; conditional E-value: 2.9e-06 GCD14 36 LelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareef 85 ++++G++v++ G Gsg l+ +a v+ tG++ +++ ++ ++ +r++ tr|A7SXL3|A7SXL3_NEMVE 74 DNIREGAKVLDVGSGSGYLTAVMAIMVGKTGKVVGIDHIQELVDMSRKNV 123 5799************************************9999999876 PP >> tr|A7SY89|A7SY89_NEMVE Predicted protein (Fragment) OS=Nematostella vectensis OX=45351 GN=v1g137654 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.2 0.0 3.2e-06 0.0086 30 95 .. 70 134 .. 45 160 .. 0.83 Alignments for each domain: == domain 1 score: 16.2 bits; conditional E-value: 3.2e-06 GCD14 30 sliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvt 95 s ++ Lel++G ++ G G+g ls+ + ++P G + +e e+ +++a +++ h ++++ tr|A7SY89|A7SY89_NEMVE 70 SEVMECLELEEGLSFLNLGSGTGYLSTMVGLIIGPSGTNHGVELFEDVVDFAHSKLQ-HFMENNPA 134 567889********************************************9998765.45555555 PP >> tr|A7RT21|A7RT21_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g240471 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -0.8 0.0 0.49 1.3e+03 184 242 .. 142 200 .. 134 205 .. 0.84 2 ! 14.4 0.0 1.2e-05 0.031 13 95 .. 194 277 .. 183 286 .. 0.79 Alignments for each domain: == domain 1 score: -0.8 bits; conditional E-value: 0.49 GCD14 184 leeidlaekrleenerkriekakrrkaskdakvedkssksstevllkPvselkghtgyL 242 l+++d +k+ +e++ + + +++r + +k+ ++ + ++++ P ++l+ ht y+ tr|A7RT21|A7RT21_NEMVE 142 LTTADKVIKHFQEESYHLVPSTDKRLYVEGSKIFAQDPHIPEVLVFPPSTDLHDHTLYI 200 6677888888888888888889999999999999999999999999999*******997 PP == domain 2 score: 14.4 bits; conditional E-value: 1.2e-05 GCD14 13 WtlaLphrtqilytaDisli.imkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvt 95 +L il + s + + + l pG+ v+++ + g s+ +a ++ G++f+f+ ++r ++++++ + + +vt tr|A7RT21|A7RT21_NEMVE 194 HDHTLYINGHILLQDKASCLpAHVISLPPGAHVIDACAAPGNKSSHLAAKLNNNGRVFSFDISASRLAVMQKQMKKAGATCVVT 277 5555555566666655555425678999*****************************************999999988766665 PP >> tr|A7SSV4|A7SSV4_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g247204 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.3 2.0 0.0002 0.55 151 209 .. 57 119 .. 49 141 .. 0.78 Alignments for each domain: == domain 1 score: 10.3 bits; conditional E-value: 0.0002 GCD14 151 qvqrtceaLrelgfteietle...vllrtyevrkskleeidlaekrleenerkri.ekakrrk 209 qr +e+ +e+ f++ietle lr+y + ++lee+ + krl++ + ++ k+k+ k tr|A7SSV4|A7SSV4_NEMVE 57 ESQREVEEEQEKLFKDIETLEdenFYLRKYVDKIQELEEMTNTSKRLDQLKDRQQrRKIKELK 119 679999**************95545689*********99999999998876553302333333 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (247 nodes) Target sequences: 24428 (8257945 residues searched) Passed MSV filter: 1174 (0.0480596); expected 488.6 (0.02) Passed bias filter: 747 (0.0305797); expected 488.6 (0.02) Passed Vit filter: 74 (0.00302931); expected 24.4 (0.001) Passed Fwd filter: 17 (0.000695923); expected 0.2 (1e-05) Initial search space (Z): 24428 [actual number of targets] Domain search space (domZ): 9 [number of targets reported over threshold] # CPU time: 0.28u 0.01s 00:00:00.29 Elapsed: 00:00:00.09 # Mc/sec: 22663.47 // [ok]