# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/GCD14.hmm.txt # target sequence database: proteomes/Saccharomyces_cerevisiae.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: GCD14 [M=247] Accession: PF08704.9 Description: tRNA methyltransferase complex GCD14 subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4e-81 270.8 0.1 2.7e-80 268.1 0.1 2.0 1 sp|P46959|TRM61_YEAST tRNA (adenine(58)-N(1))-methyltransfer 5e-06 24.8 0.1 9.4e-06 23.9 0.1 1.3 1 sp|P15646|FBRL_YEAST rRNA 2'-O-methyltransferase fibrillari ------ inclusion threshold ------ 0.015 13.5 0.0 0.024 12.8 0.0 1.3 1 sp|P40516|EFM4_YEAST Protein-lysine N-methyltransferase EFM 0.042 12.0 3.5 0.067 11.3 3.5 1.3 1 sp|Q06001|FAR10_YEAST Factor arrest protein 10 OS=Saccharomy 0.19 9.8 4.3 0.37 8.9 4.3 1.4 1 sp|P38882|UTP9_YEAST U3 small nucleolar RNA-associated prot Domain annotation for each sequence (and alignments): >> sp|P46959|TRM61_YEAST tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 OS=Saccharomyces cerevisiae # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 268.1 0.1 2.2e-83 2.7e-80 2 246 .. 72 362 .. 71 363 .. 0.86 Alignments for each domain: == domain 1 score: 268.1 bits; conditional E-value: 2.2e-83 GCD14 2 fvyvlaptPeLWtlaLphrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehe 89 fv+vl+ptPeLWtl+Lphrtqi+yt+D s i+++L+ p s+v+e+GtGsgs+sha+ar+v Ghlf+fefh+ r+e+a+eef+eh+ sp|P46959|TRM61_YEAST 72 FVHVLQPTPELWTLSLPHRTQIVYTPDSSYIMQRLNCSPHSRVIEAGTGSGSFSHAFARSV---GHLFSFEFHHIRYEQALEEFKEHG 156 9**********************************************************98...9*********************** PP GCD14 90 l.sqlvtvthrDvckeGf..de......evsg.........kvDavfLDlPaPweaiphaakal.kkeggrlCsfsPCieqvqrtcea 158 l +++vt+thrDvc+ Gf ++ e +++ vfLDlPaPw+aiph+ +++ +e+ lC+fsPCieqv +t ++ sp|P46959|TRM61_YEAST 157 LiDDNVTITHRDVCQGGFliKKgdttsyE--FgnnetaaslNANVVFLDLPAPWDAIPHLDSVIsVDEKVGLCCFSPCIEQVDKTLDV 242 9677*************975322112221..01122222225778*******************6677788***************** PP GCD14 159 Lrelgfteietlevllrtyevrkskleeidlaekrleenerkriekakrrkaskdakvedkssks..........stevllkPv.... 232 L++ g+t++e++e++ r+ye r++ ++ ++ a +rl + +r++ ++++rrk + +++++ +++k+ +++ +P+ sp|P46959|TRM61_YEAST 243 LEKYGWTDVEMVEIQGRQYESRRQMVRSLNDALERLRDIKRHKLQGVERRKRMFNNTIDSNDEKVgkrnedgvplTEKAKFNPFgkgs 330 ***************************************************9888888888888777777778874444555557777 PP GCD14 233 ..................selkghtgyLtfat 246 e+k+ht+yLtfa sp|P46959|TRM61_YEAST 331 rikegdsnykwkevtkmeAEIKSHTSYLTFAF 362 7777777777777777779***********96 PP >> sp|P15646|FBRL_YEAST rRNA 2'-O-methyltransferase fibrillarin OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 23.9 0.1 7.8e-09 9.4e-06 35 152 .. 161 279 .. 155 307 .. 0.73 Alignments for each domain: == domain 1 score: 23.9 bits; conditional E-value: 7.8e-09 GCD14 35 kLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvc.keGfdeevsgkvDavfLDlP 122 +L + pG +v+ G+ sg+ + ++ v+P G ++++ef ++ ++ + +++ ++ D+ + + + + g vD+vf D+ sp|P15646|FBRL_YEAST 161 ELFIAPGKKVLYLGAASGTSVSHVSDVVGPEGVVYAVEFSHRPGRELISMAKKR---PNIIPIIEDARhPQKY-RMLIGMVDCVFADVA 245 57799************9887889***************776555544443333...2333333454414445.4566*********** PP GCD14 123 aPwea.iphaakal..kkeggrlCsf.sPCieqv 152 +P++a i + +++ k++gg++ s + Ci+ sp|P15646|FBRL_YEAST 246 QPDQArIIALNSHMflKDQGGVVISIkANCIDST 279 ****965555566667999999998735799764 PP >> sp|P40516|EFM4_YEAST Protein-lysine N-methyltransferase EFM4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.8 0.0 2e-05 0.024 37 103 .. 90 155 .. 80 177 .. 0.88 Alignments for each domain: == domain 1 score: 12.8 bits; conditional E-value: 2e-05 GCD14 37 elkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvck 103 ++ + + vv+ GtG g + + +t + +G+l +++ e+ ++ a + + +++++++ ++ D+ + sp|P40516|EFM4_YEAST 90 RISENASVVDLGTGNGHMLFELHQT-EFQGKLVGIDYSEESVKLASNIAEATGVDNFISFQQADIFS 155 7888999**************9987.789**********************************9975 PP >> sp|Q06001|FAR10_YEAST Factor arrest protein 10 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.3 3.5 5.5e-05 0.067 148 244 .. 350 460 .. 333 463 .. 0.60 Alignments for each domain: == domain 1 score: 11.3 bits; conditional E-value: 5.5e-05 GCD14 148 CieqvqrtceaLrelgfteietlevllrtyevrkskleeidlaekrleenerkriekakrrkaskdakvedkssksstevllkPv... 232 ieq ++ +++L+ + +++ev r+++ +++++e + k+ e+er + +++ +++++k+++ ++ s k +te ++ v sp|Q06001|FAR10_YEAST 350 IIEQNRNQVKQLERDHMFFKKSFEVKKRRNNEKQKSMEREIEDLKTRLEVERYKNSQMMKKNKQKEQELSTASKKKTTEHDTRGVpgm 437 69**********988877889**********999999865443333333333333333333333333333333332333333322334 PP GCD14 233 ...........selkghtgyLtf 244 ++l h + Ltf sp|Q06001|FAR10_YEAST 438 npkgtdkfsikNTLCNHFTLLTF 460 44455555554556666666666 PP >> sp|P38882|UTP9_YEAST U3 small nucleolar RNA-associated protein 9 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 8.9 4.3 0.00031 0.37 135 226 .. 290 385 .. 280 402 .. 0.75 Alignments for each domain: == domain 1 score: 8.9 bits; conditional E-value: 0.00031 GCD14 135 lkkeggrlCsfsPCi..eqvqrtceaLrelgfteietlevllrtyev.rkskleeidl.aekrleenerkriekakrrkaskdakvedk 219 + +e+ + f+P +qv + +++e +f i+ e+ ++ y + k++ +++d+ +k+lee+e+++ +++++k++ ++k+++k sp|P38882|UTP9_YEAST 290 VVNEDLEIIDFTPINskQQVLISWLNVNEPNFESISLKEIETQGYITiNKNEKNNADEaDQKKLEEKEEEAQPEVQHEKKETETKINKK 378 556777788888865114555666778899*************9965266777788885677889999999999999999999999999 PP GCD14 220 ssksste 226 sks+++ sp|P38882|UTP9_YEAST 379 VSKSDQV 385 9996444 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (247 nodes) Target sequences: 6049 (2936363 residues searched) Passed MSV filter: 581 (0.0960489); expected 121.0 (0.02) Passed bias filter: 361 (0.0596793); expected 121.0 (0.02) Passed Vit filter: 44 (0.00727393); expected 6.0 (0.001) Passed Fwd filter: 10 (0.00165317); expected 0.1 (1e-05) Initial search space (Z): 6049 [actual number of targets] Domain search space (domZ): 5 [number of targets reported over threshold] # CPU time: 0.14u 0.00s 00:00:00.14 Elapsed: 00:00:00.06 # Mc/sec: 12088.03 // [ok]