# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/GCD14.hmm.txt # target sequence database: proteomes/Strongylocentrotus_purpuratus.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: GCD14 [M=247] Accession: PF08704.9 Description: tRNA methyltransferase complex GCD14 subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-96 324.1 0.0 1.3e-96 323.7 0.0 1.1 1 tr|W4Y137|W4Y137_STRPU tRNA (adenine(58)-N(1))-methyltransfe 4.1e-16 60.1 0.4 5.2e-10 40.1 0.0 2.6 3 tr|W4ZGG3|W4ZGG3_STRPU Uncharacterized protein OS=Strongyloc 3.8e-06 27.4 0.0 5.7e-06 26.8 0.0 1.2 1 tr|W4ZAS4|W4ZAS4_STRPU Uncharacterized protein OS=Strongyloc ------ inclusion threshold ------ 0.096 13.0 0.0 0.19 12.1 0.0 1.5 1 tr|W4YGK2|W4YGK2_STRPU Protein-L-isoaspartate O-methyltransf 0.14 12.5 0.0 0.2 12.0 0.0 1.2 1 tr|W4Z5B8|W4Z5B8_STRPU Uncharacterized protein OS=Strongyloc 0.38 11.1 5.2 0.59 10.4 5.2 1.3 1 tr|W4XSC1|W4XSC1_STRPU Uncharacterized protein OS=Strongyloc 3 8.1 7.3 4 7.7 7.3 1.3 1 tr|W4YEM5|W4YEM5_STRPU Uncharacterized protein OS=Strongyloc Domain annotation for each sequence (and alignments): >> tr|W4Y137|W4Y137_STRPU tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Strongylocentrotus pur # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 323.7 0.0 3.2e-100 1.3e-96 1 246 [. 64 325 .. 64 326 .. 0.88 Alignments for each domain: == domain 1 score: 323.7 bits; conditional E-value: 3.2e-100 GCD14 1 gfvyvlaptPeLWtlaLphrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefre 87 g+vyvl+ptPeLWt++Lphrtq+ly +Disl++ +LelkpGs vve+GtGsgs+sha++rt+aP Ghl+tfefhe+ra+ a +ef+e tr|W4Y137|W4Y137_STRPU 64 GWVYVLHPTPELWTVNLPHRTQVLYFPDISLLTIELELKPGSIVVEAGTGSGSVSHALTRTIAPDGHLYTFEFHEKRAKLAADEFKE 150 8************************************************************************************** PP GCD14 88 helsqlvtvthrDvckeGfdeevsgkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtceaLrelgfteietlevll 174 h+ls++vt thrDv ++Gf+ e + ++DavfLDlP+Pw ipha+k+lk++ggr+CsfsPCieqvqr c +L +lgf +i+t+e l+ tr|W4Y137|W4Y137_STRPU 151 HGLSHIVTATHRDVSQDGFQLENDYVADAVFLDLPSPWSVIPHAKKVLKSSGGRICSFSPCIEQVQRVCLELDQLGFSDIKTVECLM 237 *******************99999*************************************************************** PP GCD14 175 rtyevrkskleeidlaekrleenerkriekakrrka...............skdakvedkssks.......stevllkPvselkght 239 rt+evr+++l+ +l++ l n +++ + + + e+ s+s ++ +l P +e+ ght tr|W4Y137|W4Y137_STRPU 238 RTHEVRTVNLTLPNLGQPCLTPNITDHKAEEDTFELtdggarlvdlganysF-----EEPGSQSstgdcsyPKKCALAP-RETPGHT 318 ***************9999887766644444443334456666666433321.....2222222344444455666666.9****** PP GCD14 240 gyLtfat 246 gyLtfa tr|W4Y137|W4Y137_STRPU 319 GYLTFAS 325 *****96 PP >> tr|W4ZGG3|W4ZGG3_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 17.6 0.0 9.5e-07 0.0039 20 61 .. 255 296 .. 236 299 .. 0.82 2 ? -4.5 0.4 5.1 2.1e+04 196 213 .. 363 380 .. 344 397 .. 0.40 3 ! 40.1 0.0 1.3e-13 5.2e-10 20 180 .. 488 658 .. 469 679 .. 0.75 Alignments for each domain: == domain 1 score: 17.6 bits; conditional E-value: 9.5e-07 GCD14 20 rtqilytaDisliimkLelkpGsvvvesGtGsgslshaiart 61 i y +D ++ m+++ pG vv+e+G Gsgs++ + r+ tr|W4ZGG3|W4ZGG3_STRPU 255 NPVIAYPKDCCTMLMMIDACPGDVVLEAGSGSGSMTLFLPRA 296 45688******************************9887775 PP == domain 2 score: -4.5 bits; conditional E-value: 5.1 GCD14 196 enerkriekakrrkaskd 213 ++e+ +++++ ++ s++ tr|W4ZGG3|W4ZGG3_STRPU 363 DVEKFDADETNSSRLSDS 380 222222222222222111 PP == domain 3 score: 40.1 bits; conditional E-value: 1.3e-13 GCD14 20 rtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfe...fheqraekareefreh.el......sqlvtv 96 i y +D ++ m+++ pG vv+e+G Gsgs++ ++r+v+ G++ +f+ h+ raek ++ ++ ++ q+v tr|W4ZGG3|W4ZGG3_STRPU 488 NPVIAYPKDCCTMLMMIDACPGDVVLEAGSGSGSMTLFLSRAVGHHGKVLSFDklpTHQVRAEKNLKLWQTSyDIsnqvkwPQNVSF 574 45688***********************************************95556999999999888754244333233455666 PP GCD14 97 thrDvckeGfdeev.sgkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtceaLrelgftei..etlevllrtyevr 180 + ++ G ++ + +vD +D+ +P + +++ l k+g + + + v +e++r+ + + lev +++ v tr|W4ZGG3|W4ZGG3_STRPU 575 YPSRLQDCGE--HIkDAQVDGAIIDMESPSTVMTTMSQRL-KSGKAMAVYVANLTLVIDLAEVIRKERLPLVteTILEVTHKEWVVN 658 6555555554..3314589*9**************99999.6777788888888888888888887665433115678888888776 PP >> tr|W4ZAS4|W4ZAS4_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 26.8 0.0 1.4e-09 5.7e-06 35 150 .. 143 259 .. 135 270 .. 0.74 Alignments for each domain: == domain 1 score: 26.8 bits; conditional E-value: 1.4e-09 GCD14 35 kLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvc.keGfdeevsgkvDavfLD 120 ++++kpGs+v+ G+ sg+ + ++ v+P G ++++ef + r + + + +++ +++ + D+ + + + + g vD+vf D tr|W4ZAS4|W4ZAS4_STRPU 143 HIHMKPGSKVLYLGAASGTTVSHVSDLVGPEGLVYAVEFSH-RSGRDLMNVAKKRT-NIIPI-IEDARhPHKY-RMLVGMVDCVFAD 225 5789****************9******************65.44444555544443.33444.4455525556.5677********* PP GCD14 121 lPaPwea...iphaakalkkeggrlCsf.sPCie 150 + +P++a +a + lk+eg + s + Ci+ tr|W4ZAS4|W4ZAS4_STRPU 226 VAQPDQArivAINAHNFLKNEGHFVISIkANCID 259 ******9532235667788888877776256876 PP >> tr|W4YGK2|W4YGK2_STRPU Protein-L-isoaspartate O-methyltransferase OS=Strongylocentrotus purpuratus OX=7668 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.1 0.0 4.6e-05 0.19 37 94 .. 170 227 .. 157 275 .. 0.82 Alignments for each domain: == domain 1 score: 12.1 bits; conditional E-value: 4.6e-05 GCD14 37 elkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlv 94 +l +G +++ G Gsg l++ +a v+ G++ +++ ++ ++k+r++ ++++ + l+ tr|W4YGK2|W4YGK2_STRPU 170 HLSEGKAALDVGSGSGYLTSCMAIMVGSSGKVVGIDHIKELVDKSRKNIEKDNPDLLT 227 578999*****************************************99988765544 PP >> tr|W4Z5B8|W4Z5B8_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.0 0.0 4.8e-05 0.2 40 88 .. 67 115 .. 61 135 .. 0.91 Alignments for each domain: == domain 1 score: 12.0 bits; conditional E-value: 4.8e-05 GCD14 40 pGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefreh 88 + ++vv+ G G+g ai++ v+P+G + ++ +++ e ar+ +h tr|W4Z5B8|W4Z5B8_STRPU 67 EDARVVDLGSGTGRDCYAISKLVGPKGFVVGIDMTDEQLELARKYVTHH 115 5689**************************************9977766 PP >> tr|W4XSC1|W4XSC1_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.4 5.2 0.00014 0.59 168 243 .. 28 103 .. 18 107 .. 0.70 Alignments for each domain: == domain 1 score: 10.4 bits; conditional E-value: 0.00014 GCD14 168 etlevllrtyevrkskleeidlaekrleenerkriekakrrkaskdakvedkssksstevllkPvselkghtgyLt 243 et e+ + e +k+k+ +i ++ + ee++++r + ++r++ ++++e+++ + ++e l k +e + h yL tr|W4XSC1|W4XSC1_STRPU 28 ETSEIGCSRQEGKKRKVMTIWIMLQDREEEKERREAREEQRRKVTEKEKEEEEKREEEERLKKEEQEKRDHEEYLK 103 56677777788888888888885555555555555555555555555555555555899999999*********96 PP >> tr|W4YEM5|W4YEM5_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 7.7 7.3 0.00098 4 175 243 .. 63 131 .. 42 135 .. 0.70 Alignments for each domain: == domain 1 score: 7.7 bits; conditional E-value: 0.00098 GCD14 175 rtyevrkskleeidlaekrleenerkriekakrrkaskdakvedkssksstevllkPvselkghtgyLt 243 ++ e++ ++ +e +++ + ee++++r + ++r++ ++++e+++ + ++e l k +e + h yL tr|W4YEM5|W4YEM5_STRPU 63 KKLEMKAERQREREIMLQDREEEKERREAREEQRRKVTEKEKEEEEKREEEERLKKEEQEKRDHEEYLK 131 5566677777777775554455545555555555554555555555555899999999*********96 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (247 nodes) Target sequences: 28594 (14266044 residues searched) Passed MSV filter: 1810 (0.0633); expected 571.9 (0.02) Passed bias filter: 1145 (0.0400434); expected 571.9 (0.02) Passed Vit filter: 145 (0.00507099); expected 28.6 (0.001) Passed Fwd filter: 44 (0.00153878); expected 0.3 (1e-05) Initial search space (Z): 28594 [actual number of targets] Domain search space (domZ): 7 [number of targets reported over threshold] # CPU time: 0.64u 0.02s 00:00:00.66 Elapsed: 00:00:00.19 # Mc/sec: 18545.86 // [ok]