# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/GCD14.hmm.txt # target sequence database: proteomes/Tetrahymena_thermophila.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: GCD14 [M=247] Accession: PF08704.9 Description: tRNA methyltransferase complex GCD14 subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.2e-69 233.5 9.7 4.9e-64 216.9 0.1 3.0 3 tr|Q23UC8|Q23UC8_TETTS tRNA (adenine(58)-N(1))-methyltransfe 2.7e-06 27.8 0.0 3.7e-06 27.4 0.0 1.1 1 tr|I7MFA3|I7MFA3_TETTS rRNA 2'-O-methyltransferase fibrillar ------ inclusion threshold ------ 0.022 15.0 0.0 0.056 13.7 0.0 1.6 2 tr|I7M9J8|I7M9J8_TETTS NOL1/NOP2/Sun family protein OS=Tetra 0.15 12.3 0.0 0.34 11.1 0.0 1.5 1 tr|Q23PZ6|Q23PZ6_TETTS Cyclopropane-fatty-acyl-phospholipid 0.16 12.2 0.3 0.38 11.0 0.3 1.6 1 tr|W7XKT3|W7XKT3_TETTS Uncharacterized protein OS=Tetrahymen 0.59 10.3 0.0 0.59 10.3 0.0 3.1 3 tr|I7M7M0|I7M7M0_TETTS Eukaryotic initiation factor 3, gamma 3.4 7.8 7.9 9 6.5 7.9 1.7 1 tr|Q22A31|Q22A31_TETTS tRNA-dihydrouridine synthase OS=Tetra 4.5 7.4 4.7 5.1 7.3 4.7 1.1 1 tr|I7MFI9|I7MFI9_TETTS Uncharacterized protein OS=Tetrahymen 8.5 6.5 8.1 11 6.1 8.1 1.4 1 tr|W7X559|W7X559_TETTS Uncharacterized protein OS=Tetrahymen Domain annotation for each sequence (and alignments): >> tr|Q23UC8|Q23UC8_TETTS tRNA (adenine(58)-N(1))-methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -0.7 2.2 0.47 1.4e+03 196 226 .. 13 43 .. 2 68 .. 0.46 2 ! 216.9 0.1 1.6e-67 4.9e-64 2 222 .. 104 330 .. 103 344 .. 0.84 3 ! 21.1 0.1 1e-07 0.0003 188 245 .. 348 422 .. 334 424 .. 0.57 Alignments for each domain: == domain 1 score: -0.7 bits; conditional E-value: 0.47 GCD14 196 enerkriekakrrkaskdakvedkssksste 226 e++ k+ +++ +++a++ a+ e k+s+ +++ tr|Q23UC8|Q23UC8_TETTS 13 EQQVKNQNEQSQQSANQVANEEVKQSDNQQQ 43 1111222222222222223333333332333 PP == domain 2 score: 216.9 bits; conditional E-value: 1.6e-67 GCD14 2 fvyvlaptPeLWtlaLphrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefreh 88 f+y+l+p P L t +L+h tqily+aDis +i k++++pGs vvesGtGs+sls++iart++ Ghl+tfef e+ra++ e + + tr|Q23UC8|Q23UC8_TETTS 104 FIYLLRPDPVLITDTLSHLTQILYQADISYVISKMNIQPGSIVVESGTGSASLSSSIARTIQDEGHLYTFEFNEARAKNGAEVLAAQ 190 9************************************************************************************** PP GCD14 89 elsqlvtvthrDvckeGf....deev....sgkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtceaLrelgftei 167 +l+++ v rDv ++Gf e v ++++DavfLDlP P+eai ha+++l k+ggrlC+fsPCieqv + ce++r ++f++i tr|Q23UC8|Q23UC8_TETTS 191 GLKNF-DVIWRDVLSNGFmpipGERVynlkEHSADAVFLDLPRPYEAITHAKQVL-KKGGRLCCFSPCIEQVMKNCEEMRIQKFVQI 275 *9865.8899********744334553333478**********************.99***************************** PP GCD14 168 etlevllrtyevrkskleeidl.aekrleenerkriekakrrkaskdakvedkssk 222 t+ev +r +e r+++++ + + + +n+rkr ++ + +++++ +ed++++ tr|Q23UC8|Q23UC8_TETTS 276 VTIEVIQRPFERRERTFQSAFQvLNPNFINNKRKRD-EVGDNAQEQENIQEDQQQQ 330 ****************99876525555555555544.3333333444444444333 PP == domain 3 score: 21.1 bits; conditional E-value: 1e-07 GCD14 188 dlaekrleenerkriekakrrkaskdakvedkssksstevllkPv.................selkghtgyLtfa 245 d+ + +ee+++++ + k+ k++k++k+++++ + +++ +kP+ +e+kghtgyL f tr|Q23UC8|Q23UC8_TETTS 348 DEQGEDEEESQEEADQANKKGKQNKNNKKDKRNKQQNNQNGSKPIpqfirpqitkvvyssgpKEIKGHTGYLSFG 422 33333333333333333333333444444444444455666677677777777777777777**********995 PP >> tr|I7MFA3|I7MFA3_TETTS rRNA 2'-O-methyltransferase fibrillarin OS=Tetrahymena thermophila (strain SB210) OX=312017 G # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 27.4 0.0 1.2e-09 3.7e-06 34 148 .. 132 246 .. 115 267 .. 0.71 Alignments for each domain: == domain 1 score: 27.4 bits; conditional E-value: 1.2e-09 GCD14 34 mkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvckeGfdeevsgkvDavfLD 120 +++kpGs+v+ G sg+ + +a v+PtG ++++ef ++ + ++ +++ + + r ++ f + g vD vf D tr|I7MFA3|I7MFA3_TETTS 132 SDIHIKPGSKVLYLGGASGTTVSHVADIVGPTGVVYAVEFSHRSGRDLVNMAKKRTNVVPIIGDARKPQEYRF---LVGMVDVVFAD 215 5689****************9999****************654444333333333223334446666677777...7799******* PP GCD14 121 lPaPwea..iphaak.alkkeggrlCsfsPC 148 + +P++a ++ + lk+ g l s C tr|I7MFA3|I7MFA3_TETTS 216 VAQPDQAriMGMNCQyFLKNGGHFLISIKAC 246 *****99422333331346556666666543 PP >> tr|I7M9J8|I7M9J8_TETTS NOL1/NOP2/Sun family protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_00 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -3.6 0.0 3.5 1.1e+04 174 202 .. 18 47 .. 12 74 .. 0.56 2 ? 13.7 0.0 1.9e-05 0.056 33 123 .. 204 292 .. 196 298 .. 0.89 Alignments for each domain: == domain 1 score: -3.6 bits; conditional E-value: 3.5 GCD14 174 lrtyevrkskleeidl.aekrleenerkri 202 +++ e +k++ + id +++l+en+ tr|I7M9J8|I7M9J8_TETTS 18 QQELELKKEQANPIDSmLRNYLKENKALGS 47 555566666666555535555555544432 PP == domain 2 score: 13.7 bits; conditional E-value: 1.9e-05 GCD14 33 imkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvckeGfdeevsgkvDavfL 119 +k++ + G v++ +G+g s a+a ++ tG+++ + + ++a++ f++ +++ +v+ ++++ ++ +++ +k D v tr|I7M9J8|I7M9J8_TETTS 204 AFKVKCQIGDHVLDYCAGAGGKSLAFAPYLKGTGQIYLHDTRDYILQEAKKRFNKAGIQ-NVQFHTQESLNNNK-NKLVKKFDWVVC 288 57888999*************************************************95.68888888877765.788899****99 PP GCD14 120 DlPa 123 D+P tr|I7M9J8|I7M9J8_TETTS 289 DVPC 292 9995 PP >> tr|Q23PZ6|Q23PZ6_TETTS Cyclopropane-fatty-acyl-phospholipid synthase OS=Tetrahymena thermophila (strain SB210) OX=31 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.1 0.0 0.00011 0.34 38 92 .. 148 201 .. 134 229 .. 0.86 Alignments for each domain: == domain 1 score: 11.1 bits; conditional E-value: 0.00011 GCD14 38 lkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsq 92 +G +v+e G G g ++i+r ++P+ f ++f e+++++ r+ + +++ q tr|Q23PZ6|Q23PZ6_TETTS 148 NLNGLRVLEVGSGRGGGLNYISRYLNPQ-ECFGVDFSENQIRFCRHHYSQNKKLQ 201 5689***********************8.68****************99987555 PP >> tr|W7XKT3|W7XKT3_TETTS Uncharacterized protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_0000916 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.0 0.3 0.00013 0.38 153 195 .. 12 54 .. 6 85 .. 0.87 Alignments for each domain: == domain 1 score: 11.0 bits; conditional E-value: 0.00013 GCD14 153 qrtceaLrelgfteietlevllrtyevrkskleeidlaekrle 195 q +++L+e+ f++ie++e ll++ +++ s++++ d + k + tr|W7XKT3|W7XKT3_TETTS 12 QINIQKLSEQAFINIESIENLLQQIQTQYSQFQNFDYFGKDDK 54 6689******************************999665554 PP >> tr|I7M7M0|I7M7M0_TETTS Eukaryotic initiation factor 3, gamma subunit OS=Tetrahymena thermophila (strain SB210) OX=31 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 0.4 5.0 0.21 6.4e+02 140 222 .. 16 101 .. 3 121 .. 0.58 2 ? -0.2 1.1 0.32 9.5e+02 188 230 .. 140 181 .. 112 207 .. 0.76 3 ? 10.3 0.0 0.0002 0.59 130 189 .. 354 413 .. 347 419 .. 0.80 Alignments for each domain: == domain 1 score: 0.4 bits; conditional E-value: 0.21 GCD14 140 grlCsfsPCieqvqrtce.aLrelgfteietlevll.rtyevrkskleeidlaekrleener.kriekakrrkaskdakvedkssk 222 +r+ Ci+q q+ ++ +++ l+ eie e++ + ++ +++++++ + ++++++++ k e + ++++++ k+ + +++ tr|I7M7M0|I7M7M0_TETTS 16 TRFIYIQICIQQNQNKINpQINFLEMSEIEKQEIQPiIQENAEQQQVDQAPINQDNQQSDNViKGNEYVLLIDNRDNRKIVNLKEN 101 67777788******999735677788888888886413445566666666665554444433033333333444444443333333 PP == domain 2 score: -0.2 bits; conditional E-value: 0.32 GCD14 188 dlaekrleenerkriekakrrkaskdakvedkssksstevllk 230 + + +++e+ e+++ e++++r+ +d++v++ s++ + e++ k tr|I7M7M0|I7M7M0_TETTS 140 NQFYNYEENYEEEQSEQRDNRNIFNDNSVQKLSNE-EIELMKK 181 45667788888888888888888999988888887.4444433 PP == domain 3 score: 10.3 bits; conditional E-value: 0.0002 GCD14 130 haakalkkeggrlCsfsPCieqvqrtceaLr.elgfteietlevllrtyevrkskleeidl 189 + ++ +g+l ++s +e +q++ + ++ e +f++i+ +++ +r+++v k++ + i + tr|I7M7M0|I7M7M0_TETTS 354 KFMHL-LVPNGVLLAYSNYLECLQNSFDLMQqERDFVNIKIFDIFYREHQVLKNRTHPITM 413 44444.478999**************998752689***************99988776665 PP >> tr|Q22A31|Q22A31_TETTS tRNA-dihydrouridine synthase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_01 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 6.5 7.9 0.003 9 158 239 .. 57 139 .. 46 150 .. 0.74 Alignments for each domain: == domain 1 score: 6.5 bits; conditional E-value: 0.003 GCD14 158 aLrelgfteietlevllrtyevrkskleeidlaekrleenerkriekakrrkaskdakvedks.sksstevllkPvselkght 239 L+e +f++i + + + +++ ++++ + d +++++e ++++i ++ ++++ ++ ++e+++ ++ ++e+ ++ +se++ + tr|Q22A31|Q22A31_TETTS 57 LLQEEQFQNIGIIFIKMDQNQQQNHNHNSQDQLIETTQELNKQQILNFTEESQANQMNQEQQNhQQQEEEIKVNNTSEIQNYK 139 58999***************************999999888888988887777665555554404445666666666665554 PP >> tr|I7MFI9|I7MFI9_TETTS Uncharacterized protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_0066402 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 7.3 4.7 0.0017 5.1 167 227 .. 30 86 .. 6 99 .. 0.36 Alignments for each domain: == domain 1 score: 7.3 bits; conditional E-value: 0.0017 GCD14 167 ietlevllrtyevrkskleeidlaekrleenerkriekakrrkaskdakvedkssks.stev 227 ++ ++ + +++++ + +++ + ++ l++n++++ ++a++ +++++++++++ +++ tr|I7MFI9|I7MFI9_TETTS 30 LNNVDQQPNNNNINQPLINNQNNNNN-LNNNNNNSNNNANN----NNNNINNNNNNNnNNNN 86 23333333333333333333332222.22222222222222....22222222211112233 PP >> tr|W7X559|W7X559_TETTS Uncharacterized protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_0003835 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 6.1 8.1 0.0038 11 157 232 .. 27 100 .. 10 131 .. 0.64 Alignments for each domain: == domain 1 score: 6.1 bits; conditional E-value: 0.0038 GCD14 157 eaLrelgfteietlevllrtyevrkskleeidlaekrleenerkriekakrrkaskdakvedkssksstevllkPv 232 ++ +++ ei+ +e+ l++ e ++ + ++ + ++ ++++++kr k k r ++k+ +++s+s++++ +k + tr|W7X559|W7X559_TETTS 27 KECSQQTEAEINFVEIELQRLEKHTDNNKKGNKKNEGENQDKEKRKIKEKIRTR--ETKKMKEKSRSQETIQMKKI 100 445555667999999999999988888888877666666665554443333333..23333333333333333333 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (247 nodes) Target sequences: 26971 (16912921 residues searched) Passed MSV filter: 7237 (0.268325); expected 539.4 (0.02) Passed bias filter: 1794 (0.0665159); expected 539.4 (0.02) Passed Vit filter: 244 (0.00904675); expected 27.0 (0.001) Passed Fwd filter: 85 (0.00315153); expected 0.3 (1e-05) Initial search space (Z): 26971 [actual number of targets] Domain search space (domZ): 9 [number of targets reported over threshold] # CPU time: 1.41u 0.03s 00:00:01.44 Elapsed: 00:00:00.38 # Mc/sec: 10993.40 // [ok]