# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.1b2 (February 2015); http://hmmer.org/
# Copyright (C) 2015 Howard Hughes Medical Institute.
# Freely distributed under the GNU General Public License (GPLv3).
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: pfam_list/GCD14.hmm.txt
# target sequence database: proteomes/Thermococcus_kodakarensis.fasta
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: GCD14 [M=247]
Accession: PF08704.9
Description: tRNA methyltransferase complex GCD14 subunit
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
3.6e-29 99.3 0.0 1.8e-27 93.7 0.0 2.0 2 tr|Q5JGS7|Q5JGS7_THEKO tRNA (1-methyladenosine) methyltransf
3.6e-05 20.6 0.0 5.3e-05 20.1 0.0 1.2 1 sp|Q5JFN1|FLPA_THEKO Fibrillarin-like rRNA/tRNA 2'-O-methy
0.00021 18.1 0.0 0.00026 17.8 0.0 1.1 1 tr|Q5JEE1|Q5JEE1_THEKO Protein-L-isoaspartate O-methyltransf
Domain annotation for each sequence (and alignments):
>> tr|Q5JGS7|Q5JGS7_THEKO tRNA (1-methyladenosine) methyltransferase OS=Thermococcus kodakarensis (strain ATCC BAA-918
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 93.7 0.0 2.4e-30 1.8e-27 21 182 .. 74 232 .. 60 234 .. 0.93
2 ! 4.3 0.1 0.0047 3.6 228 246 .. 233 251 .. 230 252 .. 0.86
Alignments for each domain:
== domain 1 score: 93.7 bits; conditional E-value: 2.4e-30
GCD14 21 tqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvckeGfd 107
qi++ +D +ii + + pG v+e+G+Gsg+l+ +a +v+PtG++ ++e ++ +e a+++ + ++s+ v ++++ + +G d
tr|Q5JGS7|Q5JGS7_THEKO 74 PQIVHPKDAGIIIAYAGISPGDTVIEAGAGSGALTIFLANAVGPTGKVISYEVRKDFYEIAKKNVELAGFSDRVVLKNKSIY-DGID 159
59****************************************************************************9985.7995 PP
GCD14 108 eevsgkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtceaLrel..gfteietlevllrtyevrks 182
e+ ++D + LDlP+P + +pha+++l + gg + +++PC++qv r +aL+e +f+ +++evl+r+ ev+++
tr|Q5JGS7|Q5JGS7_THEKO 160 EK---EADHIVLDLPQPENVLPHAVDVL-RPGGYFVAYTPCMNQVHRFFRALEEFkgEFMRPRVVEVLVREQEVKRE 232
54...69*********************.99*********************9863369999***********9975 PP
== domain 2 score: 4.3 bits; conditional E-value: 0.0047
GCD14 228 llkPvselkghtgyLtfat 246
++P +++ +htgy+tf +
tr|Q5JGS7|Q5JGS7_THEKO 233 CMRPKTTMLAHTGYITFLR 251
579999***********76 PP
>> sp|Q5JFN1|FLPA_THEKO Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase OS=Thermococcus kodakarensis (strain ATCC BAA
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 20.1 0.0 6.9e-08 5.3e-05 35 155 .. 67 188 .. 59 211 .. 0.75
Alignments for each domain:
== domain 1 score: 20.1 bits; conditional E-value: 6.9e-08
GCD14 35 kLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvckeGfdeevsgkvDavfLDlPa 123
++ +kpGs v+ G sg+ ++ ++ v+ G++f +ef + ++ + +e+ ++v + eG+++ + kvD +f D+ +
sp|Q5JFN1|FLPA_THEKO 67 NFPIKPGSTVLYLGVASGTTASHVSDIVGWEGKVFGVEFSPRVLRELVPLVEER--RNIVPILGDATKPEGYRA-LVPKVDVIFEDVAQ 152
5679***********************************876555555444443..35677776666789**65.559*********** PP
GCD14 124 Pwea...iphaakalkkeg.grlCsfsPCieqvqrt 155
P +a i++a+ lk+ g g++ s i+ ++
sp|Q5JFN1|FLPA_THEKO 153 PTQAkilIDNAKVYLKSGGyGMISVKSRSIDVTKEP 188
**9977788888888765535565566666655554 PP
>> tr|Q5JEE1|Q5JEE1_THEKO Protein-L-isoaspartate O-methyltransferase OS=Thermococcus kodakarensis (strain ATCC BAA-918
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 17.8 0.0 3.4e-07 0.00026 30 100 .. 66 134 .. 56 162 .. 0.77
Alignments for each domain:
== domain 1 score: 17.8 bits; conditional E-value: 3.4e-07
GCD14 30 sliimkL.elkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrD 100
i+ +L +lkpG v+e GtGsg + i+ v+ ++t+e + +e+ar ++++ +++ +v+v D
tr|Q5JEE1|Q5JEE1_THEKO 66 VAIMLELaDLKPGMNVLEIGTGSGWNAALISELVK--TDVYTIERIPELVEFARRNLERAGVK-NVHVILGD 134
3444444269**************99999999886..57*******************99985.57776666 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (247 nodes)
Target sequences: 2301 (637680 residues searched)
Passed MSV filter: 56 (0.0243372); expected 46.0 (0.02)
Passed bias filter: 52 (0.0225989); expected 46.0 (0.02)
Passed Vit filter: 9 (0.00391134); expected 2.3 (0.001)
Passed Fwd filter: 3 (0.00130378); expected 0.0 (1e-05)
Initial search space (Z): 2301 [actual number of targets]
Domain search space (domZ): 3 [number of targets reported over threshold]
# CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 15750.70
//
[ok]