# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.1b2 (February 2015); http://hmmer.org/
# Copyright (C) 2015 Howard Hughes Medical Institute.
# Freely distributed under the GNU General Public License (GPLv3).
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: pfam_list/GCD14.hmm.txt
# target sequence database: proteomes/Trypanosoma_brucei.fasta
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: GCD14 [M=247]
Accession: PF08704.9
Description: tRNA methyltransferase complex GCD14 subunit
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
7.3e-66 221.3 0.7 1.8e-38 131.6 0.0 3.1 3 tr|Q383B4|Q383B4_TRYB2 tRNA (adenine(58)-N(1))-methyltransfe
2.6e-08 32.8 0.0 3.4e-08 32.4 0.0 1.1 1 tr|Q388E0|Q388E0_TRYB2 Fibrillarin, putative OS=Trypanosoma
5.3e-08 31.8 0.0 7.4e-08 31.3 0.0 1.1 1 tr|Q388C9|Q388C9_TRYB2 Fibrillarin, putative OS=Trypanosoma
1.9e-05 23.4 0.0 3.4e-05 22.6 0.0 1.3 1 tr|Q38AL4|Q38AL4_TRYB2 Fibrillarin OS=Trypanosoma brucei bru
------ inclusion threshold ------
0.039 12.6 2.4 0.11 11.1 1.2 2.4 2 tr|Q387X3|Q387X3_TRYB2 Kinesin, putative OS=Trypanosoma bruc
0.085 11.5 2.4 0.22 10.1 0.0 2.6 3 tr|Q583H8|Q583H8_TRYB2 Nucleolar protein, putative OS=Trypan
Domain annotation for each sequence (and alignments):
>> tr|Q383B4|Q383B4_TRYB2 tRNA (adenine(58)-N(1))-methyltransferase OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 131.6 0.0 1.3e-41 1.8e-38 3 109 .. 127 236 .. 126 243 .. 0.92
2 ! 78.3 0.1 2.4e-25 3.4e-22 112 179 .. 268 336 .. 261 354 .. 0.84
3 ! 9.4 0.0 0.00026 0.37 209 246 .. 341 378 .. 334 379 .. 0.72
Alignments for each domain:
== domain 1 score: 131.6 bits; conditional E-value: 1.3e-41
GCD14 3 vyvlaptPeLWtlaLphrtqilytaDisliimkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehe 89
v v++ +LWt+a+phrtqi+y +Di++i+++L+l+pG +v e+GtGsgsl+h++artvaP G ++t++fh++r +a++efr+++
tr|Q383B4|Q383B4_TRYB2 127 VLVMQNCADLWTVAVPHRTQIIYDTDIAVIVFNLRLRPGHRVAEAGTGSGSLTHSLARTVAPDGIVYTYDFHKKRCLEALQEFRRNG 213
5689999******************************************************************************** PP
GCD14 90 l.sqlvtvthrDvc..keGfdee 109
l ++lv + rDvc +G de
tr|Q383B4|Q383B4_TRYB2 214 LpASLVRCNWRDVCvtASGSDES 236
*777**********544555555 PP
== domain 2 score: 78.3 bits; conditional E-value: 2.4e-25
GCD14 112 gkvDavfLDlPaPweaiphaakalkkeggrlCsfsPCieqvqrtceaLre..lgfteietlevllrtyev 179
++vDavfLD+P+Pw ai+++ ++l + gg+lC+fsPCieq qrt+++Lr+ ++f++i+t+ev + +
tr|Q383B4|Q383B4_TRYB2 268 HHVDAVFLDVPSPWLAIDNVLHVL-RPGGMLCTFSPCIEQTQRTAQRLRQapQEFIDIRTVEVVRKFINP 336
789*********************.99*********************984469*********9886654 PP
== domain 3 score: 9.4 bits; conditional E-value: 0.00026
GCD14 209 kaskdakvedkssksstevllkPvselkghtgyLtfat 246
+++++ + + ++k + + +P ght+yLtfa+
tr|Q383B4|Q383B4_TRYB2 341 RNHSEGTEGESNNKPTCRFKFRPALVSMGHTAYLTFAR 378
3344444444555557788999***************8 PP
>> tr|Q388E0|Q388E0_TRYB2 Fibrillarin, putative OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb10
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 32.4 0.0 2.4e-11 3.4e-08 34 160 .. 62 190 .. 52 202 .. 0.82
Alignments for each domain:
== domain 1 score: 32.4 bits; conditional E-value: 2.4e-11
GCD14 34 mkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvckeGfdeevsgkvDavfLD 120
++ ++pGs v+ G+ sg+ + ++ v+P G ++++ef +r + +ee+ +++ s++v + + + ++ + vD++f+D
tr|Q388E0|Q388E0_TRYB2 62 AQIYMEPGSTVLYLGAASGTTVSHVSDLVGPEGVVYAVEFS-HRSGRDLEEMSKRR-SNIVPILEDARYPQKYRMLIPRLVDCIFMD 146
567899***************9******************5.68889999998776.566777665556889999************ PP
GCD14 121 lPaPwea...iphaakalkkeggrlCsf.sPCieqvqrtceaLr 160
+ +P++a +a + lk++gg + s + Ci+ ++ +++
tr|Q388E0|Q388E0_TRYB2 147 VAQPDQArilALNAQHFLKQNGGFVISIkANCIDSTADSATVFA 190
******95322356667899999988873679999888777655 PP
>> tr|Q388C9|Q388C9_TRYB2 Fibrillarin, putative OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb10
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 31.3 0.0 5.2e-11 7.4e-08 34 160 .. 138 266 .. 129 277 .. 0.82
Alignments for each domain:
== domain 1 score: 31.3 bits; conditional E-value: 5.2e-11
GCD14 34 mkLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvckeGfdeevsgkvDavfLD 120
++ ++pGs v+ G+ sg+ + ++ v+P G ++++ef +r + +ee+ +++ s++v + + + ++ + vD++f+D
tr|Q388C9|Q388C9_TRYB2 138 AQIYMEPGSTVLYLGAASGTTVSHVSDLVGPEGVVYAVEFS-HRSGRDLEEMSKRR-SNIVPILEDARYPQKYRMLIPRLVDCIFMD 222
567899***************9******************5.68889999998776.566777665556889999************ PP
GCD14 121 lPaPwea...iphaakalkkeggrlCsf.sPCieqvqrtceaLr 160
+ +P++a +a + lk++gg + s + Ci+ ++ +++
tr|Q388C9|Q388C9_TRYB2 223 VAQPDQArilALNAQHFLKQNGGFVISIkANCIDSTADSATVFA 266
******95322356667899999988873679998888776655 PP
>> tr|Q38AL4|Q38AL4_TRYB2 Fibrillarin OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb10.6k15.3160
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 22.6 0.0 2.4e-08 3.4e-05 35 142 .. 132 239 .. 122 258 .. 0.71
Alignments for each domain:
== domain 1 score: 22.6 bits; conditional E-value: 2.4e-08
GCD14 35 kLelkpGsvvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvc.keGfdeevsgkvDavfLD 120
++++kpGs+v+ G+ sg+ + ++ v+P G ++++ef + r + + ++ +++ ++v + D+ + + + vD++f+D
tr|Q38AL4|Q38AL4_TRYB2 132 SIHMKPGSKVLYLGAASGTTVSHVSDLVGPEGMVYAVEFSN-RSGRDLVDMSKRRP-NIVPI-IEDARyPMKY-RMLMPMVDCIFMD 214
5789****************9******************75.66666666655543.33333.3454413344.567899******* PP
GCD14 121 lPaPweaip...haakalkkeggrl 142
+ +P++a +a + lk+ g +
tr|Q38AL4|Q38AL4_TRYB2 215 VAQPDQARIlalNAQAFLKNGGHYV 239
*****99541113444455554444 PP
>> tr|Q387X3|Q387X3_TRYB2 Kinesin, putative OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb10.61.
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 11.1 1.2 7.5e-05 0.11 137 232 .. 415 512 .. 406 528 .. 0.65
2 ? -2.2 0.0 0.86 1.2e+03 49 76 .. 1453 1481 .. 1452 1492 .. 0.80
Alignments for each domain:
== domain 1 score: 11.1 bits; conditional E-value: 7.5e-05
GCD14 137 keggrlCsfsPCieqvqrtceaLre.lgfteietlevllrtyevrkskleeidl.aekrleenerkriekakrrkaskdakvedkss 221
++ +++ + sPC+e tc++Lr + ++i + v +vr++k ++++ k+l +n++ ++ + ++ v+ +++
tr|Q387X3|Q387X3_TRYB2 415 SKTSMVATVSPCVEHYEVTCQTLRYaSRAKQIVNVAVVNEDPQVRQIKKLTAEVsRLKQLLNNQSICDYSNEEVERLRQRVVQLQQE 501
6789999****************9615678899999999999999888777776333444444333333344444445555555555 PP
GCD14 222 ksstevllkPv 232
++ + +l+ +
tr|Q387X3|Q387X3_TRYB2 502 AVERDFMLETF 512
55555554444 PP
== domain 2 score: -2.2 bits; conditional E-value: 0.86
GCD14 49 tGsgslshaiartva.PtGhlftfefheq 76
t +sl+ a++r+ + +G +f fe he+
tr|Q387X3|Q387X3_TRYB2 1453 TANTSLAAAVVRAPKsALGAVFLFEQHER 1481
667899******975268********986 PP
>> tr|Q583H8|Q583H8_TRYB2 Nucleolar protein, putative OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 G
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? -1.9 0.0 0.72 1e+03 82 130 .. 122 172 .. 97 183 .. 0.59
2 ? 10.1 0.0 0.00015 0.22 42 135 .. 258 347 .. 240 366 .. 0.83
3 ? -2.0 1.7 0.76 1.1e+03 190 220 .. 473 503 .. 456 521 .. 0.46
Alignments for each domain:
== domain 1 score: -1.9 bits; conditional E-value: 0.72
GCD14 82 reefrehel.....sqlvtvthrDvc.keGfdeevsgkvDavfLDlPaPweaiph 130
+ f+e++ ++++++ D+ G++e + D+++L l +P ea++
tr|Q583H8|Q583H8_TRYB2 122 LSHFKEEREegrtrEDYMQLLRADIMeLYGYNEFL---TDSILL-LFSPTEAVEF 172
44555544333344568888888887446775444...788876.7788888875 PP
== domain 2 score: 10.1 bits; conditional E-value: 0.00015
GCD14 42 svvvesGtGsgslshaiartvaPtGhlftfefheqraekareefrehelsqlvtvthrDvckeGfdeevsgkvDavfLDlPaPweai 128
+v++ + g +++ia+ ++ tG +f+ + e r+++ ++++ ++++ v vt+ D Gf e+v ++ D ++LD P i
tr|Q583H8|Q583H8_TRYB2 258 ERVLDMAAAPGGKTTYIAQLMKNTGVIFANDVSEPRTKSLNANLQRLGVTNTV-VTNYD--GVGF-EKVMKNFDRILLDAPCTGSGI 340
5777777778888999******************************9998755.66666..569*.8899***********987777 PP
GCD14 129 phaakal 135
+ k +
tr|Q583H8|Q583H8_TRYB2 341 ISRDKSI 347
6666666 PP
== domain 3 score: -2.0 bits; conditional E-value: 0.76
GCD14 190 aekrleenerkriekakrrkaskdakvedks 220
++ e++++++ +++++r+ ++ak e k
tr|Q583H8|Q583H8_TRYB2 473 NVAKSENVKTMKGTNKRQREDDSKAKGELKT 503
2223333333333333333332222222222 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (247 nodes)
Target sequences: 8561 (4332363 residues searched)
Passed MSV filter: 388 (0.0453218); expected 171.2 (0.02)
Passed bias filter: 287 (0.0335241); expected 171.2 (0.02)
Passed Vit filter: 23 (0.0026866); expected 8.6 (0.001)
Passed Fwd filter: 7 (0.000817661); expected 0.1 (1e-05)
Initial search space (Z): 8561 [actual number of targets]
Domain search space (domZ): 6 [number of targets reported over threshold]
# CPU time: 0.11u 0.01s 00:00:00.12 Elapsed: 00:00:00.05
# Mc/sec: 21401.87
//
[ok]