# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Met_10.hmm.txt # target sequence database: proteomes/Tetrahymena_thermophila.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Met_10 [M=199] Accession: PF02475.15 Description: Met-10+ like-protein Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9.9e-53 179.4 0.2 9.9e-53 179.4 0.2 1.8 2 tr|W7XD08|W7XD08_TETTS tRNA (guanine(37)-N1)-methyltransfera 3.2e-14 53.7 0.6 9.2e-14 52.2 0.1 1.9 2 tr|I7MA21|I7MA21_TETTS tRNA (Guanine(37)-N1)-methyltransfera 4.4e-07 30.4 4.7 5e-07 30.3 1.9 2.4 2 tr|Q22MG6|Q22MG6_TETTS RNA cap guanine-N2 methyltransferase 0.00052 20.4 0.1 0.0015 18.9 0.1 1.8 1 tr|I7MGF8|I7MGF8_TETTS RNA cap guanine-N2 methyltransferase 0.0014 19.0 0.1 0.0025 18.2 0.1 1.3 1 tr|I7MKV3|I7MKV3_TETTS Arginine N-methyltransferase OS=Tetra ------ inclusion threshold ------ 0.032 14.5 0.1 0.16 12.3 0.0 2.1 2 tr|Q22HL7|Q22HL7_TETTS tRNA (guanine(26)-N(2))-dimethyltrans 0.036 14.4 0.0 0.052 13.9 0.0 1.3 1 tr|Q24FF7|Q24FF7_TETTS Methyltransferase OS=Tetrahymena ther 0.048 14.0 0.1 0.065 13.6 0.1 1.4 1 tr|I7M0Y7|I7M0Y7_TETTS Embryo-abundant protein, putative OS= 0.049 14.0 2.5 1.5 9.1 0.4 2.8 3 tr|Q22YU4|Q22YU4_TETTS Uncharacterized protein OS=Tetrahymen Domain annotation for each sequence (and alignments): >> tr|W7XD08|W7XD08_TETTS tRNA (guanine(37)-N1)-methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 G # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.1 0.2 1.4 4.1e+03 11 36 .. 19 45 .. 8 59 .. 0.48 2 ! 179.4 0.2 3.3e-56 9.9e-53 2 193 .. 124 328 .. 123 343 .. 0.94 Alignments for each domain: == domain 1 score: -2.1 bits; conditional E-value: 1.4 EEEB-T.TSSHHHHHHHHHHHHHSS-S CS Met_10 11 ilnlre.elkeykkvigevildktkvk 36 l++++ +++e + +++ ld+++vk tr|W7XD08|W7XD08_TETTS 19 ALKVENkQINEVMNGVKDQFLDIQNVK 45 333332023334444444444444444 PP == domain 2 score: 179.4 bits; conditional E-value: 3.3e-56 EEEEETTEEEEEB-TTSSHHHHHHHHHHHHHS.S-SCEEEE-S-SBTTTTB--EEEEEES-.SEEEEEETTEEEEEETTTS---GGG CS Met_10 2 kfeiiGdvvilnlreelkeykkvigevildkt.kvktvlrkvsevegefRtfelevlaGsdstetihkEngcrfklDvskvyfsskl 87 ++e++G++++ nl+ + +yk++ig+vi++k ++ktv++k ++++ ++Rt+ele++aG++++et+hkE c fklD+ kvyf s+l tr|W7XD08|W7XD08_TETTS 124 GYETVGHIAHFNLKPQQYPYKHLIGQVIIEKFkNIKTVVNKLDKLHNVYRTPELEIMAGENKLETEHKEDKCIFKLDFEKVYFCSRL 210 89****************************999****************************************************** PP HHHHHHHHTT--TT-EEEETT-TTTCCHHHCCHHTTSSEEEEEES-HHHHHHHHHHHHHTT-TTCEEEEES-GGG---TT-EE.... CS Met_10 88 eaErerlaklveegevVvdvfAGiGpfaipiakyskakrvlaidlnPesvkylkeniklNkvekvvkpileDgrevireqlad.... 170 ++Er r+ kl++++e+V+d+f G+Gp+ai +ak + +v+ +dlnP+ + yl n + N+ve++ +++D+r+v+ + tr|W7XD08|W7XD08_TETTS 211 QTERDRVLKLIKPNETVLDLFCGVGPLAIRAAK--MGCKVICNDLNPHCYDYLLINREKNNVEDKTLCFNNDARKVVDILVSKeeyk 295 *********************************..88999************************************97333335688 PP ..........EEEE--TTTGGGGHHHHHHHEEE CS Met_10 171 ..........rvvmnlpksaieFldkvlaalkd 193 +v mnlp ieF d lk+ tr|W7XD08|W7XD08_TETTS 296 kypsefhhfdHVYMNLPVDNIEFCDVFQGLLKK 328 9999999999*************9987666665 PP >> tr|I7MA21|I7MA21_TETTS tRNA (Guanine(37)-N1)-methyltransferase, putative OS=Tetrahymena thermophila (strain SB210) O # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -1.6 0.0 0.97 2.9e+03 143 176 .. 90 123 .. 85 138 .. 0.74 2 ! 52.2 0.1 3.1e-17 9.2e-14 11 109 .. 139 240 .. 129 243 .. 0.92 Alignments for each domain: == domain 1 score: -1.6 bits; conditional E-value: 0.97 HHHHTT-TTCEEEEES-GGG---TT-EEEEEE-- CS Met_10 143 niklNkvekvvkpileDgrevireqladrvvmnl 176 i+ +ve + + i++D ++ e+++++v+ n tr|I7MA21|I7MA21_TETTS 90 WIQEQNVEIIKHKIQKDISTFSLEEAIEKVINNK 123 5666677777778889999998888888888775 PP == domain 2 score: 52.2 bits; conditional E-value: 3.1e-17 EEEB-TTSSHHHHHHHHHHHHHS..S-SCEEEE-S-S.BTTTTB--EEEEEES-.SEEEEEETTEEEEEETTTS---GGGHHHHHHH CS Met_10 11 ilnlreelkeykkvigevildkt..kvktvlrkvsev.egefRtfelevlaGsdstetihkEngcrfklDvskvyfsskleaErerl 94 i++ +e+ k++k +i+ ++ldk ++ t+l+k+ + +f + ++e + G+++ + +E +c+f +D+sk y ++ ++Er r+ tr|I7MA21|I7MA21_TETTS 139 IIEFNENQKPFKDQIASIVLDKSqsSITTILQKKIDQdDEHFFVNSYEYVLGEKNFILDLQEGSCKFLIDISKFYINNYFQEERDRI 225 78999****************9878899*99987554388999******************************************** PP HTT--TT-EEEETT- CS Met_10 95 aklveegevVvdvfA 109 +l+++ e Vvd+f tr|I7MA21|I7MA21_TETTS 226 LNLINDQESVVDLFG 240 *************96 PP >> tr|Q22MG6|Q22MG6_TETTS RNA cap guanine-N2 methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=T # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -0.9 0.0 0.56 1.7e+03 117 153 .. 299 335 .. 295 343 .. 0.81 2 ! 30.3 1.9 1.7e-10 5e-07 100 174 .. 378 449 .. 371 556 .. 0.92 Alignments for each domain: == domain 1 score: -0.9 bits; conditional E-value: 0.56 HCCHHTTSSEEEEEES-HHHHHHHHHHHHHTT-TTCE CS Met_10 117 piakyskakrvlaidlnPesvkylkeniklNkvekvv 153 ++k v + ++n +s+ +l++ ++ N ++ + tr|Q22MG6|Q22MG6_TETTS 299 YCKKKESYLLVKTLEKNFQSNSFLNSQVNQNPSKQSI 335 56665666678999****************9877766 PP == domain 2 score: 30.3 bits; conditional E-value: 1.7e-10 TT-EEEETT-TTTCCHHHCCHHTTSSEEEEEES-HHHHHHHHHHHHHTT-TTCEEEEES-GGG---TT-EEEEEE CS Met_10 100 egevVvdvfAGiGpfaipiakyskakrvlaidlnPesvkylkeniklNkvekvvkpileDgrevireqladrvvm 174 ++++Vvd f G+G+ ++ ak +a++v+aid n v k ni+ k +++v++i++D ++ + +ad vv+ tr|Q22MG6|Q22MG6_TETTS 378 KNQIVVDAFCGVGYNTVQFAK--EASQVIAIDCNQVKVSHAKYNIESFKQQHKVEVIQSDFLQMK-KLKADTVVL 449 699******************..99***********************************99996.889998876 PP >> tr|I7MGF8|I7MGF8_TETTS RNA cap guanine-N2 methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=T # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 18.9 0.1 5.2e-07 0.0015 93 151 .. 335 391 .. 328 400 .. 0.90 Alignments for each domain: == domain 1 score: 18.9 bits; conditional E-value: 5.2e-07 HHHTT--TT-EEEETT-TTTCCHHHCCHHTTSSEEEEEES-HHHHHHHHHHHHHTT-TT CS Met_10 93 rlaklveegevVvdvfAGiGpfaipiakyskakrvlaidlnPesvkylkeniklNkvek 151 r a++ + e +vdv +G+f+i+ + + ++v+ai+++P +++ ++n +v++ tr|I7MGF8|I7MGF8_TETTS 335 RRASQSNRRESIVDVSCAVGQFSIAFSN--QINQVIAIEKDPIRYQFARNNASIYNVQN 391 667889999******************8..9999*****************99888876 PP >> tr|I7MKV3|I7MKV3_TETTS Arginine N-methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_00 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 18.2 0.1 8.4e-07 0.0025 99 190 .. 125 218 .. 113 227 .. 0.85 Alignments for each domain: == domain 1 score: 18.2 bits; conditional E-value: 8.4e-07 -TT-EEEETT-TTTCCHHHCCHHTTSSEEEEEES-HHHHHHHHHHHHHTT-TTCEEEEES-GGG---.TT-EEEEE...E--TTTGG CS Met_10 99 eegevVvdvfAGiGpfaipiakyskakrvlaidlnPesvkylkeniklNkvekvvkpileDgrevir.eqladrvv...mnlpksai 181 eg++V+d+ G G ++i +ak + ak v+++d n + + k +k N+++++++ i++ +ev +++d ++ m++ + tr|I7MKV3|I7MKV3_TETTS 125 FEGKIVLDIGCGTGILSIFAAK-AGAKHVYGVD-NANIILHAKAIVKNNNLADKITLIQGKMEEVELpVEKVDIIIsewMGYFLLYE 209 589******************9.88999****9.778888889999*******************8867788877766677777777 PP GGHHHHHHH CS Met_10 182 eFldkvlaa 190 + ld+vl+a tr|I7MKV3|I7MKV3_TETTS 210 SMLDCVLDA 218 777777765 PP >> tr|Q22HL7|Q22HL7_TETTS tRNA (guanine(26)-N(2))-dimethyltransferase OS=Tetrahymena thermophila (strain SB210) OX=3120 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -1.3 0.1 0.77 2.3e+03 33 53 .. 14 35 .. 3 51 .. 0.77 2 ? 12.3 0.0 5.3e-05 0.16 102 171 .. 145 226 .. 131 307 .. 0.79 Alignments for each domain: == domain 1 score: -1.3 bits; conditional E-value: 0.77 S.S-SCEEEE-S-SBTTTTB-- CS Met_10 33 t.kvktvlrkvsevegefRtfe 53 + + +tvl+++++++++ Rtf tr|Q22HL7|Q22HL7_TETTS 14 NkNKQTVLHNQTNIQAKRRTFI 35 3366899999999999999975 PP == domain 2 score: 12.3 bits; conditional E-value: 5.3e-05 -EEEETT-TTTCCHHHCCHHTTS.SEEEEEES-HHHHHHHHHHHHHTT-T.TCEEEEES-GGG---TT-EE..........E CS Met_10 102 evVvdvfAGiGpfaipiakyska.krvlaidlnPesvkylkeniklNkve.kvvkpileDgrevireqlad..........r 171 +++d + G ai +k k+ k+v+a+d+ +s k++ +n lN+++ ++++ l+D+ +++ eq++ tr|Q22HL7|Q22HL7_TETTS 145 ITILDALSASGLRAIRFSKELKDiKKVYANDISEASHKLMMDNFILNNLDlSKIEMTLKDAVQLMYEQKFRsssgdrnarfD 226 56777777778888888876665389**********************9856788999998888877666555555555542 PP >> tr|Q24FF7|Q24FF7_TETTS Methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_00865060 PE=4 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 13.9 0.0 1.7e-05 0.052 98 153 .. 63 116 .. 25 149 .. 0.82 Alignments for each domain: == domain 1 score: 13.9 bits; conditional E-value: 1.7e-05 --TT-EEEETT-TTTCCHHHCCHHTTSSEEEEEES-HHHHHHHHHHHHHTT-TTCE CS Met_10 98 veegevVvdvfAGiGpfaipiakyskakrvlaidlnPesvkylkeniklNkvekvv 153 + ++vV++v AG+G +++a+y+k +v+ d n ++++ n + + ++vv tr|Q24FF7|Q24FF7_TETTS 63 IFRDKVVLEVGAGVGLSGLVCAQYAK--QVYITDGNDIVCELMEMNAQYAQNNNVV 116 45678******************766..5688999999999999999988887777 PP >> tr|I7M0Y7|I7M0Y7_TETTS Embryo-abundant protein, putative OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHE # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 13.6 0.1 2.2e-05 0.065 94 168 .. 34 106 .. 28 258 .. 0.82 Alignments for each domain: == domain 1 score: 13.6 bits; conditional E-value: 2.2e-05 HHTT--TT-EEEETT-TTTCCHHHCCHHTTSSEEEEEES-HHHHHHHHHHHHHTT-TTCEEEEES-GGG---TT- CS Met_10 94 laklveegevVvdvfAGiGpfaipiakyskakrvlaidlnPesvkylkeniklNkvekvvkpileDgrevireql 168 + k + + e vd+ G G++ ++ + k + l+ d+ P+ ++ ++ i+ +++++vk+i D++ + + ++ tr|I7M0Y7|I7M0Y7_TETTS 34 ILKHAVDRENFVDIACGTGQLLFELSNHFK--FSLGTDISPKQIEVTNQKIQKKNLQDRVKAIVCDAHSLSKIST 106 66777788999************9999555..559*******************************999876333 PP >> tr|Q22YU4|Q22YU4_TETTS Uncharacterized protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_0012226 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -3.1 0.0 2.6 7.9e+03 151 165 .. 52 66 .. 36 76 .. 0.70 2 ? 9.1 0.4 0.00051 1.5 129 186 .. 195 257 .. 185 267 .. 0.79 3 ? 3.6 0.0 0.024 71 2 57 .. 276 333 .. 275 336 .. 0.86 Alignments for each domain: == domain 1 score: -3.1 bits; conditional E-value: 2.6 TCEEEEES-GGG--- CS Met_10 151 kvvkpileDgrevir 165 ++v+ il+D+ ++i+ tr|Q22YU4|Q22YU4_TETTS 52 NIVQKILNDCSHIIQ 66 566667777776665 PP == domain 2 score: 9.1 bits; conditional E-value: 0.00051 EEES-HHHHHHHHHHHHHTT-TTCEEEEES-GGG---..TT-EEEEEE--TTT...GGGGHHH CS Met_10 129 aidlnPesvkylkeniklNkvekvvkpileDgrevir..eqladrvvmnlpks...aieFldk 186 ++ n e +y+++ ++ + +++vk ++eD+++++r ++ r+++n ++s ++F d+ tr|Q22YU4|Q22YU4_TETTS 195 SNANNQEVLRYIQKRSRRPRADQIVKKVKEDTKNLLRnyGNAQSRFILNNMYSkrmMEKFFDN 257 677789999***************************987678889**9987541113344444 PP == domain 3 score: 3.6 bits; conditional E-value: 0.024 EEEEETTEEEEEB-TTSSHHHHHHHHHHHHHS.S-SCEEEE-S-SBTT.TTB--EEEE CS Met_10 2 kfeiiGdvvilnlreelkeykkvigevildkt.kvktvlrkvsevege.fRtfelevl 57 +fe+ ++ ++ e+++eyk + e+ +d+ + +v+ s++++e +R+++++ + tr|Q22YU4|Q22YU4_TETTS 276 NFEQFKNIWHVSNGEQYQEYKVLFREICFDFYeNHASVYVLGSQMRSEdTRKTHIKYI 333 5777788999*********************989999999999996543899998877 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (199 nodes) Target sequences: 26971 (16912921 residues searched) Passed MSV filter: 1806 (0.0669608); expected 539.4 (0.02) Passed bias filter: 504 (0.0186867); expected 539.4 (0.02) Passed Vit filter: 46 (0.00170554); expected 27.0 (0.001) Passed Fwd filter: 9 (0.000333692); expected 0.3 (1e-05) Initial search space (Z): 26971 [actual number of targets] Domain search space (domZ): 9 [number of targets reported over threshold] # CPU time: 0.29u 0.01s 00:00:00.30 Elapsed: 00:00:00.11 # Mc/sec: 30597.01 // [ok]