# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Methyltransf_15.hmm.txt # target sequence database: proteomes/Dictyostelium_discoideum.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Methyltransf_15 [M=165] Accession: PF09445.9 Description: RNA cap guanine-N2 methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.3e-32 110.6 0.2 7.6e-32 109.8 0.2 1.4 1 tr|Q54IS7|Q54IS7_DICDI Uncharacterized protein OS=Dictyostel 0.00016 20.9 0.0 0.00046 19.4 0.0 1.8 1 sp|Q54WD6|TRM5_DICDI tRNA (guanine(37)-N1)-methyltransfera 0.00021 20.5 0.1 0.00028 20.2 0.1 1.3 1 tr|Q54VE3|Q54VE3_DICDI Uncharacterized protein OS=Dictyostel 0.00095 18.4 0.2 0.002 17.4 0.0 1.6 2 tr|Q553T0|Q553T0_DICDI Uncharacterized protein OS=Dictyostel 0.0011 18.3 0.4 0.0041 16.4 0.1 2.1 2 tr|Q54C42|Q54C42_DICDI Uncharacterized protein OS=Dictyostel 0.0065 15.7 0.0 0.012 14.9 0.0 1.5 1 tr|Q54XZ7|Q54XZ7_DICDI Uncharacterized protein OS=Dictyostel ------ inclusion threshold ------ 0.014 14.6 0.1 0.049 12.9 0.1 1.8 2 tr|Q557I0|Q557I0_DICDI Uncharacterized protein OS=Dictyostel 0.021 14.0 0.0 0.048 12.9 0.0 1.6 2 sp|Q55EX9|Y8948_DICDI Putative methyltransferase DDB_G02689 0.024 13.9 0.0 0.038 13.2 0.0 1.3 1 tr|Q54JJ5|Q54JJ5_DICDI Methyltransferase type 11 domain-cont 0.1 11.8 7.2 0.091 12.0 4.2 2.2 2 tr|Q55CK9|Q55CK9_DICDI Uncharacterized protein OS=Dictyostel Domain annotation for each sequence (and alignments): >> tr|Q54IS7|Q54IS7_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0187987 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 109.8 0.2 5.9e-35 7.6e-32 2 164 .. 173 332 .. 172 333 .. 0.87 Alignments for each domain: == domain 1 score: 109.8 bits; conditional E-value: 5.9e-35 Methyltransf_15 2 evvlDlfgGaGGnviqfanvfekviaidinlsrlklaqhNaevYgv...sdkvtlvlgdvfellaklkleeikvdlvflSpPwGGps 85 ++++Dlf+GaGGn+iqf + + v+++d ++ +l +a+hN vYg + ++++v d ++l +++k d++flSpPwGGp+ tr|Q54IS7|Q54IS7_DICDI 173 NTLVDLFCGAGGNTIQFSFTCNVVVSVDLDPMKLLMAKHNSWVYGHsseNTNIEFVNSDAMNL------SNLKADVIFLSPPWGGPN 253 679*****************************************9854567888998888765......445679************ PP Methyltransf_15 86 YkkkekfdlekkleplklekllkesvklskniilfLPrnsdlkqlaalta..evlenaskvkvvklkenglvkallaylge 164 Y++ f l ++ p + +++k+++k++ n++ fLP+n+ ++a+l + + + ++ +++ + n +k+++ y+g+ tr|Q54IS7|Q54IS7_DICDI 254 YTDSSIFHL-DSMTPNGF-EIFKNAIKVTPNVVYFLPKNTSKVDIAKLALisKENGGSELCEIEENYINEKLKTITLYFGS 332 *********.99999776.599*****************99988888743113445678899***99***********985 PP >> sp|Q54WD6|TRM5_DICDI tRNA (guanine(37)-N1)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=trmt5 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 19.4 0.0 3.6e-07 0.00046 3 85 .. 216 299 .. 214 304 .. 0.92 Alignments for each domain: == domain 1 score: 19.4 bits; conditional E-value: 3.6e-07 Methyltransf_15 3 vvlDlfgGaGGnviqfanv.fekviaidinlsrlklaqhNaevYgvsdkvtlvlgdvfellaklkleeikvdlvflSpPwGGps 85 ++ D+f+G G + a++ +kv+a d n+s +k +++Na+ ++kv++ d + +++l +++i + v++ P ++ sp|Q54WD6|TRM5_DICDI 216 IICDMFAGVGPFALPAAKIkKCKVYANDLNPSSVKYMKENAKTNRLESKVEISNLDARDFVKSLVEKSIPFTHVVMNLPSTSIE 299 789*********9999998678***********************************************999999999976655 PP >> tr|Q54VE3|Q54VE3_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0205190 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 20.2 0.1 2.2e-07 0.00028 3 70 .. 49 117 .. 47 169 .. 0.87 Alignments for each domain: == domain 1 score: 20.2 bits; conditional E-value: 2.2e-07 Methyltransf_15 3 vvlDlfgGaGGnviqfanv.fekviaidinlsrlklaqhNaevYgvsdkvtlvlgdvfellaklkleei 70 vvlDl +G G vi a+ + i++din++ +k a++Na+ gv++ v +++ d+ + +kl++i tr|Q54VE3|Q54VE3_DICDI 49 VVLDLGCGDGRIVIYAAKHyGIRGIGLDINPELIKSANENAKKEGVDHLVLFKVQDFANESFDFKLSSI 117 79************9999834578999******************************996666665544 PP >> tr|Q553T0|Q553T0_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0202574 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -3.7 0.1 4.6 5.9e+03 18 38 .. 52 72 .. 43 80 .. 0.59 2 ! 17.4 0.0 1.5e-06 0.002 3 60 .. 242 300 .. 240 357 .. 0.88 Alignments for each domain: == domain 1 score: -3.7 bits; conditional E-value: 4.6 Methyltransf_15 18 fanvfekviaidinlsrlkla 38 ++ +kv +i+++ ++++ tr|Q553T0|Q553T0_DICDI 52 WTMQERKVGSIQMKATHIAKY 72 444445677777777777543 PP == domain 2 score: 17.4 bits; conditional E-value: 1.5e-06 Methyltransf_15 3 vvlDlfgGaGGnviqfanv.fekviaidinlsrlklaqhNaevYgvsdkvtlvlgdvfe 60 +vlD+ +G GG + ++ ++ +k+ +++in++++ +a++ a+ gvsd+ + +gd ++ tr|Q553T0|Q553T0_DICDI 242 LVLDVGCGVGGPTLEICQYtGCKIRGLNINKKQVGIATQRAKDLGVSDRASFDHGDAMK 300 68*************9988799**********************************987 PP >> tr|Q54C42|Q54C42_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0191845 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -1.4 0.0 0.9 1.1e+03 112 145 .. 55 88 .. 30 113 .. 0.62 2 ! 16.4 0.1 3.2e-06 0.0041 3 84 .. 430 514 .. 428 537 .. 0.77 Alignments for each domain: == domain 1 score: -1.4 bits; conditional E-value: 0.9 Methyltransf_15 112 klskniilfLPrnsdlkqlaaltaevlenaskvk 145 +l + +i+ LP+ d k+ ++++ + + +k+k tr|Q54C42|Q54C42_DICDI 55 TLHSMVIFGLPKYLDSKKFTKFLEQNNVPFKKIK 88 5555555555555555555555544444444444 PP == domain 2 score: 16.4 bits; conditional E-value: 3.2e-06 Methyltransf_15 3 vvlDlfgGaG..GnviqfanvfekviaidinlsrlklaqhNaevYgvsdkvtlvlgdvfellaklkleei...kvdlv.flSpPwGG 83 ++lD+++G+G G++i + +kvi+++i+++ + + +Na++ + d+ ++ +g+ +++l +l +++ +++ ++ pP GG tr|Q54C42|Q54C42_DICDI 430 TLLDICCGTGtiGQCIS--KKVKKVIGLEISPDAVIDSINNAKLNKI-DNAEYLVGKAEDTLPTLLTKSNggkDQEFIgIVDPPRGG 513 78********6667765..55689*******************9965.677889999888554444333332223444367788888 PP Methyltransf_15 84 p 84 tr|Q54C42|Q54C42_DICDI 514 L 514 5 PP >> tr|Q54XZ7|Q54XZ7_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0205497 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 14.9 0.0 9.2e-06 0.012 2 81 .. 143 244 .. 142 285 .. 0.85 Alignments for each domain: == domain 1 score: 14.9 bits; conditional E-value: 9.2e-06 Methyltransf_15 2 evvlDlfgGaGGnviqfanvfekvia..idinlsrlklaqhNaevYgvsdkvtlvlgdvfe....................llaklk 66 + +lDl +G+G n+f+ i+ id++ + l +a++Na +++v l d+++ ++ + tr|Q54XZ7|Q54XZ7_DICDI 143 KKILDLGTGTGCLLLSTLNEFKDSIGvgIDKSNEALIIANKNAIELSLDKRVSLLNFDWNNydknqiskllsspsssspssSSNTNN 229 6799*******9999999999887751267778888999999999999*************99***********9999997444445 PP Methyltransf_15 67 leeikvdlvflSpPw 81 +++ k+dlv+ pP tr|Q54XZ7|Q54XZ7_DICDI 230 ENNEKFDLVISNPPY 244 555578999999994 PP >> tr|Q557I0|Q557I0_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0168191 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.9 0.0 2.8 3.5e+03 110 143 .. 162 195 .. 149 207 .. 0.70 2 ? 12.9 0.1 3.8e-05 0.049 24 80 .. 253 310 .. 237 315 .. 0.91 Alignments for each domain: == domain 1 score: -2.9 bits; conditional E-value: 2.8 Methyltransf_15 110 svklskniilfLPrnsdlkqlaaltaevlenask 143 s+k ++ l+L + +l++ +++t+ + en k tr|Q557I0|Q557I0_DICDI 162 STKDQQMSKLYLGKGYELDEANQTTLIIEENGMK 195 5555566668888888888888887777666555 PP == domain 2 score: 12.9 bits; conditional E-value: 3.8e-05 Methyltransf_15 24 kviaidinlsrlklaqhNaevYgvsdkv.tlvlgdvfellaklkleeikvdlvflSpP 80 +++ id ++++ k a +N ++ g + + ++ + dvf+ l lk++++++d+++l pP tr|Q557I0|Q557I0_DICDI 253 QTYNIDESIKNFKFADRNFKLNGLDTNYhKFSQKDVFTQLDYLKSKSTRFDVIVLDPP 310 5899********************766527899***********************99 PP >> sp|Q55EX9|Y8948_DICDI Putative methyltransferase DDB_G0268948 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0268948 P # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.9 0.0 3.8e-05 0.048 3 44 .. 47 88 .. 45 118 .. 0.83 2 ? -2.6 0.0 2.2 2.7e+03 110 130 .. 186 206 .. 174 237 .. 0.70 Alignments for each domain: == domain 1 score: 12.9 bits; conditional E-value: 3.8e-05 Methyltransf_15 3 vvlDlfgGaGGnviqfanvfekviaidinlsrlklaqhNaev 44 + +D+ +G+G ++ +a++f+kvi+ + ++ +++ a++ +v sp|Q55EX9|Y8948_DICDI 47 LAIDIGAGSGQATVRLAKYFKKVIGFEPSQGQIENAEKTDNV 88 579***************************999998875544 PP == domain 2 score: -2.6 bits; conditional E-value: 2.2 Methyltransf_15 110 svklskniilfLPrnsdlkql 130 s++ + + + LP++ ++++ sp|Q55EX9|Y8948_DICDI 186 SFENTTRKTISLPKSMSINDM 206 455555556667777777766 PP >> tr|Q54JJ5|Q54JJ5_DICDI Methyltransferase type 11 domain-containing protein OS=Dictyostelium discoideum OX=44689 GN=D # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 13.2 0.0 3e-05 0.038 3 40 .. 45 82 .. 43 109 .. 0.87 Alignments for each domain: == domain 1 score: 13.2 bits; conditional E-value: 3e-05 Methyltransf_15 3 vvlDlfgGaGGnviqfanvfekviaidinlsrlklaqh 40 + +D +G+G ++++a++f+kvi+ + ++ +++ a + tr|Q54JJ5|Q54JJ5_DICDI 45 LAIDCGCGSGQATVKLAKYFKKVIGFEPSQGQIENAVK 82 579************************99999887765 PP >> tr|Q55CK9|Q55CK9_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0190741 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.0 4.2 7.1e-05 0.091 10 97 .. 32 130 .. 25 180 .. 0.76 2 ? 0.2 0.0 0.29 3.7e+02 119 144 .. 229 254 .. 209 291 .. 0.77 Alignments for each domain: == domain 1 score: 12.0 bits; conditional E-value: 7.1e-05 Methyltransf_15 10 GaG.GnviqfanvfekviaidinlsrlklaqhNaevYgvsdkvtlvlgdvfe............llaklkleeikvdlvflSpPwGG 83 G G G +++fa+ +kvi i n+++lk + +N ++ ++k +++ d+++ + + k+++ v++++ G tr|Q55CK9|Q55CK9_DICDI 32 GIGlGITNEFAKKGCKVILIGRNENKLKNIIENIKIQFGNEKCYYICSDLTKeedfknckqeieNI-SNKFGQY-VEILVNCAGSGT 116 5555899*****************************989**********99888777776654311.1133333.566666666666 PP Methyltransf_15 84 psYkkkekfdlekk 97 +Y ++++f +++ tr|Q55CK9|Q55CK9_DICDI 117 WKYIEDTSFKECNE 130 66666666665554 PP == domain 2 score: 0.2 bits; conditional E-value: 0.29 Methyltransf_15 119 lfLPrnsdlkqlaaltaevlena.skv 144 +LP+ + ++q+++ t++ +en+ +++ tr|Q55CK9|Q55CK9_DICDI 229 KYLPK-VSIDQISKATISAIENRkQRI 254 58997.679999998887665541455 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (165 nodes) Target sequences: 12739 (6856946 residues searched) Passed MSV filter: 888 (0.0697072); expected 254.8 (0.02) Passed bias filter: 216 (0.0169558); expected 254.8 (0.02) Passed Vit filter: 30 (0.00235497); expected 12.7 (0.001) Passed Fwd filter: 10 (0.000784991); expected 0.1 (1e-05) Initial search space (Z): 12739 [actual number of targets] Domain search space (domZ): 10 [number of targets reported over threshold] # CPU time: 0.12u 0.01s 00:00:00.13 Elapsed: 00:00:00.06 # Mc/sec: 18856.60 // [ok]