# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Methyltransf_4.hmm.txt # target sequence database: proteomes/Petromyzon_marinus.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Methyltransf_4 [M=173] Accession: PF02390.16 Description: Putative methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.3e-33 113.1 0.1 8.1e-33 112.9 0.1 1.0 1 tr|S4R6J3|S4R6J3_PETMA tRNA (guanine(46)-N(7))-methyltransfe 2.9e-05 23.2 0.0 0.026 13.6 0.0 2.8 3 tr|S4R4I3|S4R4I3_PETMA Phosphoethanolamine methyltransferase 0.00036 19.6 0.0 0.0024 16.9 0.0 2.2 1 tr|S4RJ28|S4RJ28_PETMA Zgc:162396 OS=Petromyzon marinus OX=7 0.00037 19.6 0.0 0.0023 17.0 0.0 2.0 1 tr|S4RNK8|S4RNK8_PETMA Zgc:162396 OS=Petromyzon marinus OX=7 0.00043 19.4 0.0 0.00062 18.9 0.0 1.2 1 tr|S4R931|S4R931_PETMA Zgc:103625 OS=Petromyzon marinus OX=7 0.0038 16.3 0.0 0.0059 15.7 0.0 1.2 1 tr|S4RMF5|S4RMF5_PETMA HemK methyltransferase family member 0.0052 15.9 0.0 0.85 8.7 0.0 2.6 2 tr|S4RMG0|S4RMG0_PETMA Phosphoethanolamine methyltransferase 0.0073 15.4 0.0 0.16 11.0 0.0 2.2 2 tr|S4RFT9|S4RFT9_PETMA Zgc:162396 OS=Petromyzon marinus OX=7 ------ inclusion threshold ------ 0.036 13.1 0.4 0.11 11.5 0.0 2.1 3 tr|S4RRK5|S4RRK5_PETMA Methyltransferase-like protein OS=Pet 0.041 13.0 0.1 2.8 7.0 0.0 2.3 2 tr|S4RQZ8|S4RQZ8_PETMA THUMP domain containing 2 OS=Petromyz 0.062 12.4 0.0 0.12 11.4 0.0 1.5 1 tr|S4R827|S4R827_PETMA Protein-L-isoaspartate (D-aspartate) 0.081 12.0 0.0 0.11 11.6 0.0 1.2 1 tr|S4RFJ6|S4RFJ6_PETMA Uncharacterized protein OS=Petromyzon 0.092 11.8 0.0 0.21 10.7 0.0 1.6 1 tr|S4RXY4|S4RXY4_PETMA Methyltransferase-like protein OS=Pet Domain annotation for each sequence (and alignments): >> tr|S4R6J3|S4R6J3_PETMA tRNA (guanine(46)-N(7))-methyltransferase OS=Petromyzon marinus OX=7757 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 112.9 0.1 7.8e-36 8.1e-33 72 172 .. 1 101 [. 1 102 [. 0.98 Alignments for each domain: == domain 1 score: 112.9 bits; conditional E-value: 7.8e-36 SEEEEEEES-----SGGGGGGSTTSHHHHHHHHHHEEEEEEEEEEESHHHHHHHHHHHHHCSTTEEESSCCCSCCTCHCHHCCCSST CS Methyltransf_4 72 slekifilFPDPwfKkrhhKrRllqpellkelarvLkkgGvlelaTDveeyaeemlkvleeeplferlskeeeleeeplsvlriatk 158 +l+k+f+lFPDP+fKk +hK+R+++p+ll+e+a++L++gG+++++TDv e++++mlk++ee+plf+ + +e + + ++s+l + t+ tr|S4R6J3|S4R6J3_PETMA 1 QLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYAYILRPGGLVYTITDVHEVHRWMLKHFEEHPLFHHVPLDELEDDVIVSQLGECTE 87 699************************************************************************************ PP TCCCCCCTTSS-EE CS Methyltransf_4 159 yeqkvqskgkeiye 172 +++kv+++g+e++ tr|S4R6J3|S4R6J3_PETMA 88 EGKKVTRNGGEKFT 101 **********9986 PP >> tr|S4R4I3|S4R4I3_PETMA Phosphoethanolamine methyltransferase OS=Petromyzon marinus OX=7757 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 6.5 0.0 0.0037 3.9 4 77 .. 53 119 .. 50 122 .. 0.68 2 ? -3.7 0.0 5.3 5.5e+03 99 115 .. 135 151 .. 134 152 .. 0.86 3 ! 13.6 0.0 2.5e-05 0.026 4 50 .. 286 331 .. 283 355 .. 0.83 Alignments for each domain: == domain 1 score: 6.5 bits; conditional E-value: 0.0037 EEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSSEEEEES-CCCHHHHCS-TTSEEEEE CS Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkNlrilrgdalkvllelledgslekif 77 vle+G G G+f+ +lAk+ +++ +++ ++ ++k + +++ N++ l+ d ++l ++++s++ if tr|S4R4I3|S4R4I3_PETMA 53 KVLELGAGIGRFTGHLAKQARHVT--AVDFMQTFLDKN--RELNSDHVNIKFLQADV-TQL--QFPENSFDVIF 119 689****************87765..666666666652..22233556777777774.555..56666666665 PP == domain 2 score: -3.7 bits; conditional E-value: 5.3 HHHHHHHHEEEEEEEEE CS Methyltransf_4 99 llkelarvLkkgGvlel 115 l++ + r L++gG l + tr|S4R4I3|S4R4I3_PETMA 135 LVNSMLRWLRPGGHLFF 151 6899999******9987 PP == domain 3 score: 13.6 bits; conditional E-value: 2.5e-05 EEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTT CS Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealel 50 vl++GcG G+ +Ak++ ++++lG+++++++v+ +++++ ++ tr|S4R4I3|S4R4I3_PETMA 286 KVLDVGCGIGGGNFYMAKAF-KVDVLGMDLSSNMVEIAMERATEDST 331 589***************98.89*****************9988755 PP >> tr|S4RJ28|S4RJ28_PETMA Zgc:162396 OS=Petromyzon marinus OX=7757 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.9 0.0 2.3e-06 0.0024 5 85 .. 2 74 .. 1 145 [. 0.81 Alignments for each domain: == domain 1 score: 16.9 bits; conditional E-value: 2.3e-06 EEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSSEEEEES-CCCHHHHCS-TTSEEEEEEES----- CS Methyltransf_4 5 vleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkNlrilrgdalkvllelledgslekifilFPDPwf 85 ++iGcG+G+ + lA ++n+lGi+++ + ++++ ++a++ N+ ++ g a ++l + dgs++ + + + wf tr|S4RJ28|S4RJ28_PETMA 2 AVDIGCGSGQGTEILASF--STNVLGIDVSPSQIEEA---MHAKHPDNISYIVGPA-ETL--PVDDGSVSLLTAFVSAHWF 74 69**********999975..789*******9987765...6789999****99999.887..4667777766666666665 PP >> tr|S4RNK8|S4RNK8_PETMA Zgc:162396 OS=Petromyzon marinus OX=7757 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 17.0 0.0 2.2e-06 0.0023 5 86 .. 2 75 .. 1 145 [. 0.81 Alignments for each domain: == domain 1 score: 17.0 bits; conditional E-value: 2.2e-06 EEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSSEEEEES-CCCHHHHCS-TTSEEEEEEES-----S CS Methyltransf_4 5 vleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkNlrilrgdalkvllelledgslekifilFPDPwfK 86 +++GcG+G+ + lA +++lGi+++ + ++++ ++a++l N+ ++ g a ++l + dgs++ + + + wf tr|S4RNK8|S4RNK8_PETMA 2 AVDVGCGSGQGTELLASIS--TKVLGIDVSPSQIEEA---MRAKHLDNITYIVGPA-ETL--PVDDGSVSLLTAFVSAHWFD 75 69*************9875..789******9987765...67899********999.888..46777777776666666654 PP >> tr|S4R931|S4R931_PETMA Zgc:103625 OS=Petromyzon marinus OX=7757 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 18.9 0.0 6e-07 0.00062 5 54 .. 30 79 .. 26 85 .. 0.95 Alignments for each domain: == domain 1 score: 18.9 bits; conditional E-value: 6e-07 EEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSSEE CS Methyltransf_4 5 vleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkNlr 54 le+ +G+G+++v+lA ++P+ ++ E++ ++ + + + ++a +l+N++ tr|S4R931|S4R931_PETMA 30 ALEVASGSGQHVVHLARAFPHLTWQPSELEASSFQSISAYARATQLQNVL 79 68**********************************************85 PP >> tr|S4RMF5|S4RMF5_PETMA HemK methyltransferase family member 1 OS=Petromyzon marinus OX=7757 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 15.7 0.0 5.7e-06 0.0059 5 51 .. 62 108 .. 60 121 .. 0.91 Alignments for each domain: == domain 1 score: 15.7 bits; conditional E-value: 5.7e-06 EEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTS CS Methyltransf_4 5 vleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelk 51 vl+iGcG+G + +a+ + P ++++++++ + va + +++e+l l+ tr|S4RMF5|S4RMF5_PETMA 62 VLDIGCGSGAISLAILSASPLASCVAVDKSADAVALARENAEDLSLQ 108 79*********************************999999888765 PP >> tr|S4RMG0|S4RMG0_PETMA Phosphoethanolamine methyltransferase OS=Petromyzon marinus OX=7757 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 4.5 0.0 0.016 16 4 72 .. 50 111 .. 47 119 .. 0.68 2 ! 8.7 0.0 0.00082 0.85 4 73 .. 284 349 .. 281 354 .. 0.82 Alignments for each domain: == domain 1 score: 4.5 bits; conditional E-value: 0.016 EEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSSEEEEES-CCCHHHHCS-TTS CS Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkNlrilrgdalkvllelledgs 72 +le+G G G+f+ +lAk +++ +++ ++ +ak k ++ + Nl l+ d ++l ++ +s tr|S4RMG0|S4RMG0_PETMA 50 DILELGAGIGRFTGHLAKIANHVT--AVDFMENFIAKN--KEDNGHRGNLTFLQADV-TQL--DFPANS 111 589***************987665..677777777663..44444556777777774.444..455555 PP == domain 2 score: 8.7 bits; conditional E-value: 0.00082 EEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSSEEEEES-CCCHHHHCS-TTSE CS Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkNlrilrgdalkvllelledgsl 73 vl++GcG G+ ++k +++lG+++++++va +++++ ++++ ++ +da+k ++++++s+ tr|S4RMG0|S4RMG0_PETMA 284 KVLDVGCGIGGGDFLMSKLY-GVEVLGVDLSSNMVAIAMERAVEEDAPLVQFEISDATK---RVFPENSF 349 689********999999998.589******************99998888887788744...34666655 PP >> tr|S4RFT9|S4RFT9_PETMA Zgc:162396 OS=Petromyzon marinus OX=7757 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 11.0 0.0 0.00015 0.16 4 60 .. 44 95 .. 42 104 .. 0.82 2 ? 2.2 0.0 0.081 85 97 115 .. 120 138 .. 117 172 .. 0.90 Alignments for each domain: == domain 1 score: 11.0 bits; conditional E-value: 0.00015 EEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSSEEEEES-C CS Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkNlrilrgda 60 + +++GcG+G+ + l+ P ++G+++++ +++++ + ++ +N++++ g a tr|S4RFT9|S4RFT9_PETMA 44 LAVDVGCGSGQCTRLLSRCCPR--VVGVDVSEAMIREATRV---NRTENIAYTVGTA 95 679****************996..69********9998653...4566777777766 PP == domain 2 score: 2.2 bits; conditional E-value: 0.081 HHHHHHHHHHEEEEEEEEE CS Methyltransf_4 97 pellkelarvLkkgGvlel 115 p ++ e+arvL++gG l + tr|S4RFT9|S4RFT9_PETMA 120 PDFVREAARVLQPGGCLAV 138 689************9976 PP >> tr|S4RRK5|S4RRK5_PETMA Methyltransferase-like protein OS=Petromyzon marinus OX=7757 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.9 0.5 2.9 3e+03 154 168 .. 57 71 .. 41 92 .. 0.50 2 ? 11.5 0.0 0.00011 0.11 5 46 .. 185 227 .. 181 262 .. 0.77 3 ? -2.6 0.0 2.4 2.5e+03 98 115 .. 274 291 .. 272 297 .. 0.83 Alignments for each domain: == domain 1 score: -2.9 bits; conditional E-value: 2.9 CCSSTTCCCCCCTTS CS Methyltransf_4 154 riatkyeqkvqskgk 168 + +t+ e+ +++k + tr|S4RRK5|S4RRK5_PETMA 57 QWTTEQEEEARHKVQ 71 333333333333222 PP == domain 2 score: 11.5 bits; conditional E-value: 0.00011 EEEET-TTSHHH..HHHHHHSTTSEEEEEES-HHHHHHHHHHHH CS Methyltransf_4 5 vleiGcGkGefl..valAkknPdknflGiEirvkvvakvlkkie 46 +leiGcG G+ + + + +++Pd+ + G + +++ v+ v ++ + tr|S4RRK5|S4RRK5_PETMA 185 ILEIGCGAGNTIfpILQTNNDPDVFVYGCDFSQSAVELVKSH-Q 227 79*******965227789999**********99999765444.3 PP == domain 3 score: -2.6 bits; conditional E-value: 2.4 HHHHHHHHHEEEEEEEEE CS Methyltransf_4 98 ellkelarvLkkgGvlel 115 + ++ l r Lk+gGv+ + tr|S4RRK5|S4RRK5_PETMA 274 AAVTRLSRLLKPGGVFLF 291 568889999*****9876 PP >> tr|S4RQZ8|S4RQZ8_PETMA THUMP domain containing 2 OS=Petromyzon marinus OX=7757 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 7.0 0.0 0.0027 2.8 4 46 .. 270 312 .. 267 327 .. 0.89 2 ? 3.7 0.1 0.028 29 96 115 .. 360 379 .. 354 381 .. 0.89 Alignments for each domain: == domain 1 score: 7.0 bits; conditional E-value: 0.0027 EEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHH CS Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkie 46 vv++ cG+G++l++ A + ++ +lG ++ + +a++ ++i+ tr|S4RQZ8|S4RQZ8_PETMA 270 VVMDPMCGMGTLLLEAAMAWKTVRCLGTDVDAQQLAHAKENIH 312 566777***************************9999998886 PP == domain 2 score: 3.7 bits; conditional E-value: 0.028 SHHHHHHHHHHEEEEEEEEE CS Methyltransf_4 96 qpellkelarvLkkgGvlel 115 p++l+e++rvL++gG+++l tr|S4RQZ8|S4RQZ8_PETMA 360 MPKILQEMMRVLRVGGNIVL 379 6899*************976 PP >> tr|S4R827|S4R827_PETMA Protein-L-isoaspartate (D-aspartate) O-methyltransferase OS=Petromyzon marinus OX=7757 PE=4 S # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.4 0.0 0.00012 0.12 4 50 .. 83 130 .. 80 139 .. 0.88 Alignments for each domain: == domain 1 score: 11.4 bits; conditional E-value: 0.00012 EEEEET-TTSHHHHHHHHHST.TSEEEEEES-HHHHHHHHHHHHHCTT CS Methyltransf_4 4 vvleiGcGkGeflvalAkknP.dknflGiEirvkvvakvlkkiealel 50 vl++G+G+G ++ +A + + +++Gi+ +++v++++k+++++++ tr|S4R827|S4R827_PETMA 83 SVLDVGSGSGYLTACIARMVGsSGKVFGIDHIQELVDEAIKNVNKDDM 130 689**************99751679******************99876 PP >> tr|S4RFJ6|S4RFJ6_PETMA Uncharacterized protein OS=Petromyzon marinus OX=7757 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.6 0.0 0.0001 0.11 100 165 .. 54 119 .. 40 125 .. 0.94 Alignments for each domain: == domain 1 score: 11.6 bits; conditional E-value: 0.0001 HHHHHHHEEEEEEEEEEESHHHHHHHHHHHHHCSTTEEESSCCCSCCTCHCHHCCCSSTTCCCCCC CS Methyltransf_4 100 lkelarvLkkgGvlelaTDveeyaeemlkvleeeplferlskeeeleeeplsvlriatkyeqkvqs 165 l++++ +L + G++ l D++ + e+ + l + ++f + ++ + ee s+lr+a ++ +kvq tr|S4RFJ6|S4RFJ6_PETMA 54 LQDAQVKLFVTGLFSLNHDIPAFKEHLRDFLVQIKEFAGEDTSDLYLEERESSLRQANEEKRKVQM 119 7889999*******************************************************9985 PP >> tr|S4RXY4|S4RXY4_PETMA Methyltransferase-like protein OS=Petromyzon marinus OX=7757 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.7 0.0 0.0002 0.21 2 80 .. 93 171 .. 92 172 .. 0.89 Alignments for each domain: == domain 1 score: 10.7 bits; conditional E-value: 0.0002 -EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSSEEEEES-CCCHHHHCS-TTSEEEEEEES CS Methyltransf_4 2 apvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkNlrilrgdalkvllelledgslekifilF 80 + vvle GcG G+ l l +++P++ + + + + + v+ v ++ ++ + l+ + ++ l e +++g+++ + + F tr|S4RXY4|S4RXY4_PETMA 93 RLVVLEAGCGVGNCLFPLLEEDPNIFVYACDFSPRAVDFVKSNPLYSKERCLAFQCDLTQNSLVENVPEGTVDAVTLIF 171 6799*********************************999998888888888888888888888888888888888877 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (173 nodes) Target sequences: 13539 (5048892 residues searched) Passed MSV filter: 323 (0.023857); expected 270.8 (0.02) Passed bias filter: 249 (0.0183913); expected 270.8 (0.02) Passed Vit filter: 44 (0.00324987); expected 13.5 (0.001) Passed Fwd filter: 13 (0.000960189); expected 0.1 (1e-05) Initial search space (Z): 13539 [actual number of targets] Domain search space (domZ): 13 [number of targets reported over threshold] # CPU time: 0.09u 0.00s 00:00:00.09 Elapsed: 00:00:00.04 # Mc/sec: 21836.46 // [ok]