# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.1b2 (February 2015); http://hmmer.org/
# Copyright (C) 2015 Howard Hughes Medical Institute.
# Freely distributed under the GNU General Public License (GPLv3).
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: pfam_list/Methyltransf_4.hmm.txt
# target sequence database: proteomes/Tetrahymena_thermophila.fasta
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: Methyltransf_4 [M=173]
Accession: PF02390.16
Description: Putative methyltransferase
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
8.3e-50 169.2 0.0 1.4e-49 168.5 0.0 1.3 1 tr|Q23MB9|Q23MB9_TETTS tRNA (guanine-N(7)-)-methyltransferas
3.2e-06 27.3 0.9 4.7e-06 26.8 0.2 1.6 2 tr|Q22BT0|Q22BT0_TETTS RNA cap guanine-N2 methyltransferase
0.00014 22.0 0.7 0.00039 20.5 0.7 1.7 1 tr|I7M1S8|I7M1S8_TETTS tRNA (Uracil-5)-methyltransferase OS=
0.00018 21.6 1.5 0.0069 16.4 0.0 2.2 2 tr|I7M0Y7|I7M0Y7_TETTS Embryo-abundant protein, putative OS=
0.00043 20.4 0.4 0.0043 17.1 0.1 2.2 2 tr|W7XCF3|W7XCF3_TETTS HemK methyltransferase family protein
0.00045 20.3 0.0 0.00076 19.6 0.0 1.3 1 tr|Q239D4|Q239D4_TETTS Cyclopropane-fatty-acyl-phospholipid
0.0018 18.4 0.1 0.016 15.3 0.1 2.7 2 tr|I7MGH3|I7MGH3_TETTS RNA cap guanine-N2 methyltransferase
------ inclusion threshold ------
0.014 15.4 0.0 0.043 13.9 0.0 1.8 1 tr|Q245D9|Q245D9_TETTS UbiE/COQ5 family methyltransferase OS
0.015 15.3 0.7 0.029 14.4 0.0 1.8 2 tr|I7M2L9|I7M2L9_TETTS Methyltransferase OS=Tetrahymena ther
Domain annotation for each sequence (and alignments):
>> tr|Q23MB9|Q23MB9_TETTS tRNA (guanine-N(7)-)-methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 168.5 0.0 4.7e-53 1.4e-49 2 172 .. 115 292 .. 114 293 .. 0.99
Alignments for each domain:
== domain 1 score: 168.5 bits; conditional E-value: 4.7e-53
-EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC.......TTSSEEEEES-CCCHHHHCS-TTSEEEEEEES- CS
Methyltransf_4 2 apvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal.......elkNlrilrgdalkvllelledgslekifilFP 81
+pv+l+iGcG G+++ +lAk++P+k++lG+Eir k+v++v +ki+al +++N +++r++a++++ +++++g++ek+f++F
tr|Q23MB9|Q23MB9_TETTS 115 QPVFLDIGCGFGGLVFNLAKNFPEKLVLGLEIRDKLVNYVSEKIKALriespdhKYNNAACIRTNAMRHIIQYFRKGTIEKMFFCFA 201
89************************************************************************************* PP
----SGGGGGGSTTSHHHHHHHHHHEEEEEEEEEEESHHHHHHHHHHHHHCSTTEEESSCCCSCCTCHCHHCCCSSTTCCCCCCTTS CS
Methyltransf_4 82 DPwfKkrhhKrRllqpellkelarvLkkgGvlelaTDveeyaeemlkvleeeplferlskeeeleeeplsvlriatkyeqkvqskgk 168
DP+fKk h+rR+++ +l+e+a Lk++G ++ +TDve+++++ ++l+++ f +ls+ee + + +++ ++ +t+++qkv+++g+
tr|Q23MB9|Q23MB9_TETTS 202 DPHFKKSNHRRRIINTGFLSEYAFLLKPKGRIYCITDVEDLHNWQEEHLAKHRMFRKLSEEELVGDICVQLIQDSTEEGQKVTRNGG 288
*************************************************************************************** PP
S-EE CS
Methyltransf_4 169 eiye 172
+++
tr|Q23MB9|Q23MB9_TETTS 289 KKWT 292
9996 PP
>> tr|Q22BT0|Q22BT0_TETTS RNA cap guanine-N2 methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=T
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 26.8 0.2 1.6e-09 4.7e-06 4 73 .. 40 107 .. 37 109 .. 0.88
2 ? -2.8 0.0 1.9 5.6e+03 149 149 .. 247 247 .. 217 268 .. 0.48
Alignments for each domain:
== domain 1 score: 26.8 bits; conditional E-value: 1.6e-09
EEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC.TTSSEEEEES-CCCHHHHCS-TTSE CS
Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal.elkNlrilrgdalkvllelledgsl 73
l++GcG G++l +l ++ k +G +i++k v +++ki+++ e++N+++++ da + + ++le++sl
tr|Q22BT0|Q22BT0_TETTS 40 NYLDVGCGTGQMLFKLNSHF--KFAVGTDISEKQVSVANEKIANNqEYSNVKVIQCDA-NNIISSLEKNSL 107
579*************9887..899****************988779***********.555588888876 PP
== domain 2 score: -2.8 bits; conditional E-value: 1.9
H CS
Methyltransf_4 149 p 149
+
tr|Q22BT0|Q22BT0_TETTS 247 T 247
1 PP
>> tr|I7M1S8|I7M1S8_TETTS tRNA (Uracil-5)-methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHE
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 20.5 0.7 1.3e-07 0.00039 2 68 .. 532 596 .. 531 602 .. 0.89
Alignments for each domain:
== domain 1 score: 20.5 bits; conditional E-value: 1.3e-07
-EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSSEEEEES-CCCHHHHCS CS
Methyltransf_4 2 apvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkNlrilrgdalkvllell 68
+ ++l+i++G G++ + lA n k+++GiE+ ++ + + +ie+++++N +++++ +v++e++
tr|I7M1S8|I7M1S8_TETTS 532 KTIFLDICSGIGTIGICLA--NSAKEVIGIEMVETACNDAKINIEQNGITNYKVICSKVEDVINEIV 596
679*********9877776..67899******************************99888887765 PP
>> tr|I7M0Y7|I7M0Y7_TETTS Embryo-abundant protein, putative OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHE
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 16.4 0.0 2.3e-06 0.0069 3 60 .. 42 98 .. 40 110 .. 0.84
2 ? 3.9 0.6 0.016 48 112 171 .. 210 266 .. 199 268 .. 0.80
Alignments for each domain:
== domain 1 score: 16.4 bits; conditional E-value: 2.3e-06
EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSS.EEEEES-C CS
Methyltransf_4 3 pvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkN.lrilrgda 60
+++i cG G++l +l++++ k lG +i+ k ++ ++ki++++l+ ++ + da
tr|I7M0Y7|I7M0Y7_TETTS 42 ENFVDIACGTGQLLFELSNHF--KFSLGTDISPKQIEVTNQKIQKKNLQDrVKAIVCDA 98
679***************997..899********************8874355555555 PP
== domain 2 score: 3.9 bits; conditional E-value: 0.016
EEEEEESHHHHHHHHHHHHHCSTTEEESSCCCSCCTCHCHHCCCSSTTCCCCCCTTSS-E CS
Methyltransf_4 112 vlelaTDveeyaeemlkvleeeplferlskeeeleeeplsvlriatkyeqkvqskgkeiy 171
+++++T + em+k l+ + ++ l ++++++ + + ++ k+e+ v++k +ei
tr|I7M0Y7|I7M0Y7_TETTS 210 NVQTVTNTPCSIFEMVKYLKTYSAYNTLLEKNKNNTNFI---DPVIKFEEDVTKKVQEIQ 266
6677777777788999******99999999988876655...9********999998876 PP
>> tr|W7XCF3|W7XCF3_TETTS HemK methyltransferase family protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 17.1 0.1 1.4e-06 0.0043 2 71 .. 116 187 .. 115 199 .. 0.88
2 ? 0.9 0.0 0.14 4.1e+02 102 120 .. 239 257 .. 232 283 .. 0.74
Alignments for each domain:
== domain 1 score: 17.1 bits; conditional E-value: 1.4e-06
-EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC..TTSSEEEEES-CCCHHHHCS-TT CS
Methyltransf_4 2 apvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal..elkNlrilrgdalkvllelledg 71
+ +leiG G G + + l k+n + + ++i++++ ++ + ++ + + + + l++++ + lk++++ l+++
tr|W7XCF3|W7XCF3_TETTS 116 KCQFLEIGIGTGAISLSLLKENSQFTGIAIDKQKFCIELAKQNLNLNkiDPQRLQLIHLECLKFFQNNLNQN 187
5679******************************999999888776656999***************99876 PP
== domain 2 score: 0.9 bits; conditional E-value: 0.14
HHHHHEEEEEEEEEEESHH CS
Methyltransf_4 102 elarvLkkgGvlelaTDve 120
++Lk+gG++ l Dv
tr|W7XCF3|W7XCF3_TETTS 239 YGYQILKQGGYIALEVDVT 257
55689***********954 PP
>> tr|Q239D4|Q239D4_TETTS Cyclopropane-fatty-acyl-phospholipid synthase OS=Tetrahymena thermophila (strain SB210) OX=31
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 19.6 0.0 2.5e-07 0.00076 5 54 .. 53 108 .. 50 126 .. 0.83
Alignments for each domain:
== domain 1 score: 19.6 bits; conditional E-value: 2.5e-07
EEEET-TTSHHHHHHHHHSTTS.EEEEEES-HHHHHHHHHHHHHC.....TTSSEE CS
Methyltransf_4 5 vleiGcGkGeflvalAkknPdk.nflGiEirvkvvakvlkkieal.....elkNlr 54
+l+iG G G l ++A k+P++ ++lG++i ++ v+++ k +++ +l+N++
tr|Q239D4|Q239D4_TETTS 53 ILDIGIGTGLPLYKIASKFPQNaKILGVDIDENYVKAAQKLFKDKknveiRLQNFY 108
79******************863799************999988855555666654 PP
>> tr|I7MGH3|I7MGH3_TETTS RNA cap guanine-N2 methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=T
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 15.3 0.1 5.2e-06 0.016 3 60 .. 321 378 .. 319 391 .. 0.92
2 ? -1.8 0.0 0.96 2.9e+03 96 116 .. 553 573 .. 552 609 .. 0.70
Alignments for each domain:
== domain 1 score: 15.3 bits; conditional E-value: 5.2e-06
EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC..TTSSEEEEES-C CS
Methyltransf_4 3 pvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal..elkNlrilrgda 60
p++++ +cG G+ +v+ +k n k +++++ + + +++++++i+++ +++N++ ++ d
tr|I7MGH3|I7MGH3_TETTS 321 PILIDACCGIGGNTVQFSKIN--KFVFAVDQSYRAIKTCNENIKNHkqDVSNVEFIQADF 378
899***************997..89******************999889*****999885 PP
== domain 2 score: -1.8 bits; conditional E-value: 0.96
SHHHHHHHHHHEEEEEEEEEE CS
Methyltransf_4 96 qpellkelarvLkkgGvlela 116
q++ll+ + + L+k+G+++ +
tr|I7MGH3|I7MGH3_TETTS 553 QKNLLNSFSDELEKEGIIHQK 573
567778888888888888754 PP
>> tr|Q245D9|Q245D9_TETTS UbiE/COQ5 family methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTH
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 13.9 0.0 1.4e-05 0.043 2 73 .. 51 120 .. 50 124 .. 0.86
Alignments for each domain:
== domain 1 score: 13.9 bits; conditional E-value: 1.4e-05
-EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSS.EEEEES-CCCHHHHCS-TTSE CS
Methyltransf_4 2 apvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkN.lrilrgdalkvllelledgsl 73
++ l+i cG G++l +l +++ + +G +i++k + v + i+ ++l+N ++ ++ d ++l+++l++++l
tr|Q245D9|Q245D9_TETTS 51 KKAYLDIACGTGQLLFQLQNHF--EKSIGTDISEKQLSVVEELIKSNNLSNsVYSIKADC-HQLPNILQEKNL 120
56789************97666..678***********************9566677775.999999998876 PP
>> tr|I7M2L9|I7M2L9_TETTS Methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_00305590 PE=4
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 14.4 0.0 9.6e-06 0.029 3 47 .. 52 94 .. 50 108 .. 0.85
2 ? -2.8 0.3 1.9 5.6e+03 144 144 .. 236 236 .. 208 262 .. 0.53
Alignments for each domain:
== domain 1 score: 14.4 bits; conditional E-value: 9.6e-06
EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHH CS
Methyltransf_4 3 pvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiea 47
++l+iGcG+G + +l+++n ++Gi+i++++++ ++++ +
tr|I7M2L9|I7M2L9_TETTS 52 QLILDIGCGSGLSGAQLSEHNH--HWVGIDISRDMLNVAVERESE 94
689****************996..579*******99999887665 PP
== domain 2 score: -2.8 bits; conditional E-value: 1.9
S CS
Methyltransf_4 144 e 144
+
tr|I7M2L9|I7M2L9_TETTS 236 Q 236
1 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (173 nodes)
Target sequences: 26971 (16912921 residues searched)
Passed MSV filter: 3057 (0.113344); expected 539.4 (0.02)
Passed bias filter: 492 (0.0182418); expected 539.4 (0.02)
Passed Vit filter: 52 (0.001928); expected 27.0 (0.001)
Passed Fwd filter: 9 (0.000333692); expected 0.3 (1e-05)
Initial search space (Z): 26971 [actual number of targets]
Domain search space (domZ): 9 [number of targets reported over threshold]
# CPU time: 0.25u 0.01s 00:00:00.26 Elapsed: 00:00:00.11
# Mc/sec: 26599.41
//
[ok]