# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Methyltransf_4.hmm.txt # target sequence database: proteomes/Tetrahymena_thermophila.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Methyltransf_4 [M=173] Accession: PF02390.16 Description: Putative methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.3e-50 169.2 0.0 1.4e-49 168.5 0.0 1.3 1 tr|Q23MB9|Q23MB9_TETTS tRNA (guanine-N(7)-)-methyltransferas 3.2e-06 27.3 0.9 4.7e-06 26.8 0.2 1.6 2 tr|Q22BT0|Q22BT0_TETTS RNA cap guanine-N2 methyltransferase 0.00014 22.0 0.7 0.00039 20.5 0.7 1.7 1 tr|I7M1S8|I7M1S8_TETTS tRNA (Uracil-5)-methyltransferase OS= 0.00018 21.6 1.5 0.0069 16.4 0.0 2.2 2 tr|I7M0Y7|I7M0Y7_TETTS Embryo-abundant protein, putative OS= 0.00043 20.4 0.4 0.0043 17.1 0.1 2.2 2 tr|W7XCF3|W7XCF3_TETTS HemK methyltransferase family protein 0.00045 20.3 0.0 0.00076 19.6 0.0 1.3 1 tr|Q239D4|Q239D4_TETTS Cyclopropane-fatty-acyl-phospholipid 0.0018 18.4 0.1 0.016 15.3 0.1 2.7 2 tr|I7MGH3|I7MGH3_TETTS RNA cap guanine-N2 methyltransferase ------ inclusion threshold ------ 0.014 15.4 0.0 0.043 13.9 0.0 1.8 1 tr|Q245D9|Q245D9_TETTS UbiE/COQ5 family methyltransferase OS 0.015 15.3 0.7 0.029 14.4 0.0 1.8 2 tr|I7M2L9|I7M2L9_TETTS Methyltransferase OS=Tetrahymena ther Domain annotation for each sequence (and alignments): >> tr|Q23MB9|Q23MB9_TETTS tRNA (guanine-N(7)-)-methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 168.5 0.0 4.7e-53 1.4e-49 2 172 .. 115 292 .. 114 293 .. 0.99 Alignments for each domain: == domain 1 score: 168.5 bits; conditional E-value: 4.7e-53 -EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC.......TTSSEEEEES-CCCHHHHCS-TTSEEEEEEES- CS Methyltransf_4 2 apvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal.......elkNlrilrgdalkvllelledgslekifilFP 81 +pv+l+iGcG G+++ +lAk++P+k++lG+Eir k+v++v +ki+al +++N +++r++a++++ +++++g++ek+f++F tr|Q23MB9|Q23MB9_TETTS 115 QPVFLDIGCGFGGLVFNLAKNFPEKLVLGLEIRDKLVNYVSEKIKALriespdhKYNNAACIRTNAMRHIIQYFRKGTIEKMFFCFA 201 89************************************************************************************* PP ----SGGGGGGSTTSHHHHHHHHHHEEEEEEEEEEESHHHHHHHHHHHHHCSTTEEESSCCCSCCTCHCHHCCCSSTTCCCCCCTTS CS Methyltransf_4 82 DPwfKkrhhKrRllqpellkelarvLkkgGvlelaTDveeyaeemlkvleeeplferlskeeeleeeplsvlriatkyeqkvqskgk 168 DP+fKk h+rR+++ +l+e+a Lk++G ++ +TDve+++++ ++l+++ f +ls+ee + + +++ ++ +t+++qkv+++g+ tr|Q23MB9|Q23MB9_TETTS 202 DPHFKKSNHRRRIINTGFLSEYAFLLKPKGRIYCITDVEDLHNWQEEHLAKHRMFRKLSEEELVGDICVQLIQDSTEEGQKVTRNGG 288 *************************************************************************************** PP S-EE CS Methyltransf_4 169 eiye 172 +++ tr|Q23MB9|Q23MB9_TETTS 289 KKWT 292 9996 PP >> tr|Q22BT0|Q22BT0_TETTS RNA cap guanine-N2 methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=T # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 26.8 0.2 1.6e-09 4.7e-06 4 73 .. 40 107 .. 37 109 .. 0.88 2 ? -2.8 0.0 1.9 5.6e+03 149 149 .. 247 247 .. 217 268 .. 0.48 Alignments for each domain: == domain 1 score: 26.8 bits; conditional E-value: 1.6e-09 EEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC.TTSSEEEEES-CCCHHHHCS-TTSE CS Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal.elkNlrilrgdalkvllelledgsl 73 l++GcG G++l +l ++ k +G +i++k v +++ki+++ e++N+++++ da + + ++le++sl tr|Q22BT0|Q22BT0_TETTS 40 NYLDVGCGTGQMLFKLNSHF--KFAVGTDISEKQVSVANEKIANNqEYSNVKVIQCDA-NNIISSLEKNSL 107 579*************9887..899****************988779***********.555588888876 PP == domain 2 score: -2.8 bits; conditional E-value: 1.9 H CS Methyltransf_4 149 p 149 + tr|Q22BT0|Q22BT0_TETTS 247 T 247 1 PP >> tr|I7M1S8|I7M1S8_TETTS tRNA (Uracil-5)-methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHE # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 20.5 0.7 1.3e-07 0.00039 2 68 .. 532 596 .. 531 602 .. 0.89 Alignments for each domain: == domain 1 score: 20.5 bits; conditional E-value: 1.3e-07 -EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSSEEEEES-CCCHHHHCS CS Methyltransf_4 2 apvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkNlrilrgdalkvllell 68 + ++l+i++G G++ + lA n k+++GiE+ ++ + + +ie+++++N +++++ +v++e++ tr|I7M1S8|I7M1S8_TETTS 532 KTIFLDICSGIGTIGICLA--NSAKEVIGIEMVETACNDAKINIEQNGITNYKVICSKVEDVINEIV 596 679*********9877776..67899******************************99888887765 PP >> tr|I7M0Y7|I7M0Y7_TETTS Embryo-abundant protein, putative OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHE # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.4 0.0 2.3e-06 0.0069 3 60 .. 42 98 .. 40 110 .. 0.84 2 ? 3.9 0.6 0.016 48 112 171 .. 210 266 .. 199 268 .. 0.80 Alignments for each domain: == domain 1 score: 16.4 bits; conditional E-value: 2.3e-06 EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSS.EEEEES-C CS Methyltransf_4 3 pvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkN.lrilrgda 60 +++i cG G++l +l++++ k lG +i+ k ++ ++ki++++l+ ++ + da tr|I7M0Y7|I7M0Y7_TETTS 42 ENFVDIACGTGQLLFELSNHF--KFSLGTDISPKQIEVTNQKIQKKNLQDrVKAIVCDA 98 679***************997..899********************8874355555555 PP == domain 2 score: 3.9 bits; conditional E-value: 0.016 EEEEEESHHHHHHHHHHHHHCSTTEEESSCCCSCCTCHCHHCCCSSTTCCCCCCTTSS-E CS Methyltransf_4 112 vlelaTDveeyaeemlkvleeeplferlskeeeleeeplsvlriatkyeqkvqskgkeiy 171 +++++T + em+k l+ + ++ l ++++++ + + ++ k+e+ v++k +ei tr|I7M0Y7|I7M0Y7_TETTS 210 NVQTVTNTPCSIFEMVKYLKTYSAYNTLLEKNKNNTNFI---DPVIKFEEDVTKKVQEIQ 266 6677777777788999******99999999988876655...9********999998876 PP >> tr|W7XCF3|W7XCF3_TETTS HemK methyltransferase family protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN= # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 17.1 0.1 1.4e-06 0.0043 2 71 .. 116 187 .. 115 199 .. 0.88 2 ? 0.9 0.0 0.14 4.1e+02 102 120 .. 239 257 .. 232 283 .. 0.74 Alignments for each domain: == domain 1 score: 17.1 bits; conditional E-value: 1.4e-06 -EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC..TTSSEEEEES-CCCHHHHCS-TT CS Methyltransf_4 2 apvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal..elkNlrilrgdalkvllelledg 71 + +leiG G G + + l k+n + + ++i++++ ++ + ++ + + + + l++++ + lk++++ l+++ tr|W7XCF3|W7XCF3_TETTS 116 KCQFLEIGIGTGAISLSLLKENSQFTGIAIDKQKFCIELAKQNLNLNkiDPQRLQLIHLECLKFFQNNLNQN 187 5679******************************999999888776656999***************99876 PP == domain 2 score: 0.9 bits; conditional E-value: 0.14 HHHHHEEEEEEEEEEESHH CS Methyltransf_4 102 elarvLkkgGvlelaTDve 120 ++Lk+gG++ l Dv tr|W7XCF3|W7XCF3_TETTS 239 YGYQILKQGGYIALEVDVT 257 55689***********954 PP >> tr|Q239D4|Q239D4_TETTS Cyclopropane-fatty-acyl-phospholipid synthase OS=Tetrahymena thermophila (strain SB210) OX=31 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 19.6 0.0 2.5e-07 0.00076 5 54 .. 53 108 .. 50 126 .. 0.83 Alignments for each domain: == domain 1 score: 19.6 bits; conditional E-value: 2.5e-07 EEEET-TTSHHHHHHHHHSTTS.EEEEEES-HHHHHHHHHHHHHC.....TTSSEE CS Methyltransf_4 5 vleiGcGkGeflvalAkknPdk.nflGiEirvkvvakvlkkieal.....elkNlr 54 +l+iG G G l ++A k+P++ ++lG++i ++ v+++ k +++ +l+N++ tr|Q239D4|Q239D4_TETTS 53 ILDIGIGTGLPLYKIASKFPQNaKILGVDIDENYVKAAQKLFKDKknveiRLQNFY 108 79******************863799************999988855555666654 PP >> tr|I7MGH3|I7MGH3_TETTS RNA cap guanine-N2 methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=T # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 15.3 0.1 5.2e-06 0.016 3 60 .. 321 378 .. 319 391 .. 0.92 2 ? -1.8 0.0 0.96 2.9e+03 96 116 .. 553 573 .. 552 609 .. 0.70 Alignments for each domain: == domain 1 score: 15.3 bits; conditional E-value: 5.2e-06 EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC..TTSSEEEEES-C CS Methyltransf_4 3 pvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal..elkNlrilrgda 60 p++++ +cG G+ +v+ +k n k +++++ + + +++++++i+++ +++N++ ++ d tr|I7MGH3|I7MGH3_TETTS 321 PILIDACCGIGGNTVQFSKIN--KFVFAVDQSYRAIKTCNENIKNHkqDVSNVEFIQADF 378 899***************997..89******************999889*****999885 PP == domain 2 score: -1.8 bits; conditional E-value: 0.96 SHHHHHHHHHHEEEEEEEEEE CS Methyltransf_4 96 qpellkelarvLkkgGvlela 116 q++ll+ + + L+k+G+++ + tr|I7MGH3|I7MGH3_TETTS 553 QKNLLNSFSDELEKEGIIHQK 573 567778888888888888754 PP >> tr|Q245D9|Q245D9_TETTS UbiE/COQ5 family methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTH # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 13.9 0.0 1.4e-05 0.043 2 73 .. 51 120 .. 50 124 .. 0.86 Alignments for each domain: == domain 1 score: 13.9 bits; conditional E-value: 1.4e-05 -EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSS.EEEEES-CCCHHHHCS-TTSE CS Methyltransf_4 2 apvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkN.lrilrgdalkvllelledgsl 73 ++ l+i cG G++l +l +++ + +G +i++k + v + i+ ++l+N ++ ++ d ++l+++l++++l tr|Q245D9|Q245D9_TETTS 51 KKAYLDIACGTGQLLFQLQNHF--EKSIGTDISEKQLSVVEELIKSNNLSNsVYSIKADC-HQLPNILQEKNL 120 56789************97666..678***********************9566677775.999999998876 PP >> tr|I7M2L9|I7M2L9_TETTS Methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_00305590 PE=4 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 14.4 0.0 9.6e-06 0.029 3 47 .. 52 94 .. 50 108 .. 0.85 2 ? -2.8 0.3 1.9 5.6e+03 144 144 .. 236 236 .. 208 262 .. 0.53 Alignments for each domain: == domain 1 score: 14.4 bits; conditional E-value: 9.6e-06 EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHH CS Methyltransf_4 3 pvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiea 47 ++l+iGcG+G + +l+++n ++Gi+i++++++ ++++ + tr|I7M2L9|I7M2L9_TETTS 52 QLILDIGCGSGLSGAQLSEHNH--HWVGIDISRDMLNVAVERESE 94 689****************996..579*******99999887665 PP == domain 2 score: -2.8 bits; conditional E-value: 1.9 S CS Methyltransf_4 144 e 144 + tr|I7M2L9|I7M2L9_TETTS 236 Q 236 1 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (173 nodes) Target sequences: 26971 (16912921 residues searched) Passed MSV filter: 3057 (0.113344); expected 539.4 (0.02) Passed bias filter: 492 (0.0182418); expected 539.4 (0.02) Passed Vit filter: 52 (0.001928); expected 27.0 (0.001) Passed Fwd filter: 9 (0.000333692); expected 0.3 (1e-05) Initial search space (Z): 26971 [actual number of targets] Domain search space (domZ): 9 [number of targets reported over threshold] # CPU time: 0.25u 0.01s 00:00:00.26 Elapsed: 00:00:00.11 # Mc/sec: 26599.41 // [ok]