# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Methyltransf_4.hmm.txt # target sequence database: proteomes/Trypanosoma_brucei.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Methyltransf_4 [M=173] Accession: PF02390.16 Description: Putative methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.4e-37 125.2 0.0 1.1e-36 124.9 0.0 1.1 1 tr|Q38E77|Q38E77_TRYB2 tRNA (guanine-N(7)-)-methyltransferas 0.00017 20.1 0.0 0.00035 19.0 0.0 1.5 1 tr|Q4GYR8|Q4GYR8_TRYB2 Uncharacterized protein OS=Trypanosom 0.0021 16.5 0.0 0.0026 16.2 0.0 1.1 1 tr|Q4GYL1|Q4GYL1_TRYB2 Uncharacterized protein OS=Trypanosom 0.0038 15.7 0.0 0.0056 15.1 0.0 1.3 1 tr|Q57Z13|Q57Z13_TRYB2 Uncharacterized protein OS=Trypanosom 0.0072 14.8 0.0 0.017 13.6 0.0 1.6 2 tr|Q585P9|Q585P9_TRYB2 rRNA adenine N(6)-methyltransferase O ------ inclusion threshold ------ 0.014 13.8 0.0 0.03 12.7 0.0 1.5 1 tr|Q389Z0|Q389Z0_TRYB2 Uncharacterized protein OS=Trypanosom 0.016 13.6 0.0 0.04 12.3 0.0 1.8 2 tr|Q386J1|Q386J1_TRYB2 Histone-lysine N-methyltransferase, H 0.02 13.4 0.0 0.039 12.4 0.0 1.5 1 tr|Q4GZF2|Q4GZF2_TRYB2 Histone-lysine N-methyltransferase, H 0.051 12.0 0.0 0.077 11.4 0.0 1.3 1 tr|Q581Z0|Q581Z0_TRYB2 Histone-lysine N-methyltransferase, H Domain annotation for each sequence (and alignments): >> tr|Q38E77|Q38E77_TRYB2 tRNA (guanine-N(7)-)-methyltransferase OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 124.9 0.0 1.1e-39 1.1e-36 4 153 .. 54 214 .. 51 238 .. 0.90 Alignments for each domain: == domain 1 score: 124.9 bits; conditional E-value: 1.1e-39 EEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC...........TTSSEEEEES-CCCHHHHCS-TTSEEEEEEE CS Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal...........elkNlrilrgdalkvllelledgslekifil 79 +++++Gc+ G++l lA+++Pd+ +lG+Eir kvv+ + +k+ +l +++N+ + + +k+ ++ +++g+l+ +f++ tr|Q38E77|Q38E77_TRYB2 54 TLVDVGCAFGGMLFSLAPDFPDMCMLGLEIRPKVVEFAQNKVMKLregaatdgtshHYRNVWFEQLNVMKFGSNCFNKGQLQALFFC 140 579************************************************************************************ PP S-----SGGGGGGSTTSHHHHHHHHHHEEEEEEEEEEESHHHHHHHHHHHHHCSTTEEESSCCCSCCTCHCHHC CS Methyltransf_4 80 FPDPwfKkrhhKrRllqpellkelarvLkkgGvlelaTDveeyaeemlkvleeeplferlskeeeleeeplsvl 153 +PDP+ K++ +rR+++p l++e+a+ Lk+gG+l +++Dvee+ ++m++ l+ plf +ls+ee +e+++++ tr|Q38E77|Q38E77_TRYB2 141 YPDPHWKRKNVRRRIISPGLVQEYAYWLKVGGFLFTVSDVEELERWMVTCLDGCPLFRQLSQEELAGNEMVRKM 214 **************************************************************988777666443 PP >> tr|Q4GYR8|Q4GYR8_TRYB2 Uncharacterized protein OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=TB # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 19.0 0.0 3.7e-07 0.00035 3 69 .. 398 462 .. 396 467 .. 0.89 Alignments for each domain: == domain 1 score: 19.0 bits; conditional E-value: 3.7e-07 EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHCTTSSEEEEES-CCCHHHHCS- CS Methyltransf_4 3 pvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkiealelkNlrilrgdalkvllelle 69 ++l+++cG G++ +alAk+ k ++GiE+ ++ v+ + ++e+++++N +g ++l+++++ tr|Q4GYR8|Q4GYR8_TRYB2 398 TTLLDLCCGTGTIGIALAKHV--KRVIGIELVESAVRDARLNAERNGVRNATFNCGRVEHLLPSVIS 462 5789*********99999985..899*************************9999998777777776 PP >> tr|Q4GYL1|Q4GYL1_TRYB2 Uncharacterized protein OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=TB # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.2 0.0 2.7e-06 0.0026 86 135 .. 19 69 .. 17 74 .. 0.84 Alignments for each domain: == domain 1 score: 16.2 bits; conditional E-value: 2.7e-06 SGGGGGGSTTSHH.HHHHHHHHEEEEEEEEEEESHHHHHHHHHHHHHCSTT CS Methyltransf_4 86 KkrhhKrRllqpe.llkelarvLkkgGvlelaTDveeyaeemlkvleeepl 135 K++++K+Rl + + + + L gG + l+TD + y + ++v + p+ tr|Q4GYL1|Q4GYL1_TRYB2 19 KRQKKKKRLWGTTnTAGGVRTLLYRGGGILLVTDCFHYLPHLMQVGTCFPS 69 889999***976515667888899*******************99988765 PP >> tr|Q57Z13|Q57Z13_TRYB2 Uncharacterized protein OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 15.1 0.0 5.9e-06 0.0056 53 133 .. 159 245 .. 109 259 .. 0.76 Alignments for each domain: == domain 1 score: 15.1 bits; conditional E-value: 5.9e-06 EEEEES-CCCHHHHCS-TTSEEEEEEES-----SGGGGGGSTTSHHHHHHHHHHEEEE.......EEEEEEESHHHHHHHHHHHHHC CS Methyltransf_4 53 lrilrgdalkvllelledgslekifilFPDPwfKkrhhKrRllqpellkelarvLkkg.......GvlelaTDveeyaeemlkvlee 132 + +r+da+ +l++ll++ + ++ P P+ ++ +Rl+++ + ++r+Lk++ G++ + TD ++ae m+++l e tr|Q57Z13|Q57Z13_TRYB2 159 TQFVRSDAFFLLQQLLPPSVADTCVVPMPVPFWSEQSSHQRLVTRDFFCVIHRILKEResptdprGFV-TFTDAVPLAEFMMEQLME 244 56799**************************************************9752222222555.569999999999988877 PP S CS Methyltransf_4 133 e 133 tr|Q57Z13|Q57Z13_TRYB2 245 S 245 6 PP >> tr|Q585P9|Q585P9_TRYB2 rRNA adenine N(6)-methyltransferase OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX= # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 13.6 0.0 1.8e-05 0.017 4 63 .. 91 149 .. 88 160 .. 0.83 2 ? -3.5 0.0 3 2.8e+03 87 112 .. 304 329 .. 301 332 .. 0.77 Alignments for each domain: == domain 1 score: 13.6 bits; conditional E-value: 1.8e-05 EEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC.TTSSEEEEES-CCCH CS Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal.elkNlrilrgdalkv 63 +vleiG G G+++ +l + + k++++ E+ ++va+++k+ +++ + l+++rg+ l++ tr|Q585P9|Q585P9_TRYB2 91 IVLEIGPGTGNLTEKLLQ--AAKKVIAFEVDPRMVAELNKRFQNSpLAPKLQVIRGNCLDH 149 89***********99965..6799********************95445788888887655 PP == domain 2 score: -3.5 bits; conditional E-value: 3 GGGGGGSTTSHHHHHHHHHHEEEEEE CS Methyltransf_4 87 krhhKrRllqpellkelarvLkkgGv 112 +r+h +lq+ + ++ arvL ++ + tr|Q585P9|Q585P9_TRYB2 304 FRQHLESILQEPIFDKRARVLDQESI 329 68888889999999999999887765 PP >> tr|Q389Z0|Q389Z0_TRYB2 Uncharacterized protein OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.7 0.0 3.2e-05 0.03 5 48 .. 214 257 .. 210 280 .. 0.82 Alignments for each domain: == domain 1 score: 12.7 bits; conditional E-value: 3.2e-05 EEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC CS Methyltransf_4 5 vleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal 48 vl+++cG G + +a+Ak+ P+ ++ +++i + + + ++++ + tr|Q389Z0|Q389Z0_TRYB2 214 VLDMCCGTGCVGIAIAKHVPSAQVTAVDILDEAIGASEENAKLN 257 89***************************999998877766655 PP >> tr|Q386J1|Q386J1_TRYB2 Histone-lysine N-methyltransferase, H3 lysine-79 specific OS=Trypanosoma brucei brucei (strai # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.6 0.0 1.7 1.6e+03 6 29 .. 33 55 .. 30 62 .. 0.76 2 ? 12.3 0.0 4.2e-05 0.04 3 45 .. 152 194 .. 150 244 .. 0.87 Alignments for each domain: == domain 1 score: -2.6 bits; conditional E-value: 1.7 EEET-TTSHHHHHHHHHSTTSEEE CS Methyltransf_4 6 leiGcGkGeflvalAkknPdknfl 29 + +G++ G +lv l +k+ + f+ tr|Q386J1|Q386J1_TRYB2 33 VGVGSAIGALLVYLNNKQTHS-FI 55 678999999999998887653.44 PP == domain 2 score: 12.3 bits; conditional E-value: 4.2e-05 EEEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHH CS Methyltransf_4 3 pvvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkki 45 v+++iG+G G++ ++l + +++G+E+ ++ +++ + tr|Q386J1|Q386J1_TRYB2 152 AVFCDIGSGVGNVCLQLLAETNCRKVVGVEVIPSRHRAATTAF 194 68**************999999999******988877776555 PP >> tr|Q4GZF2|Q4GZF2_TRYB2 Histone-lysine N-methyltransferase, H3 lysine-79 specific OS=Trypanosoma brucei brucei (strai # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.4 0.0 4.1e-05 0.039 4 65 .. 114 183 .. 111 193 .. 0.73 Alignments for each domain: == domain 1 score: 12.4 bits; conditional E-value: 4.1e-05 EEEEET-TTSHHHHHHHHHSTTSEEEEEES-HHHHHHHHHHHHHC.........TTSSEEEEES-CCCHHH CS Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirvkvvakvlkkieal.........elkNlrilrgdalkvll 65 ++ ++GcG+G++l + A + +++G+Ei+++ + + ++ +l + + i++gd kvl+ tr|Q4GZF2|Q4GZF2_TRYB2 114 TFYDLGCGNGSVLFHVALATG-ASCVGVEINEHNAKVAKEAWTHLrpvfekrrgRKLDVSIVCGDFCKVLK 183 689**************9875.689*****98766555555444455566666655566777777766665 PP >> tr|Q581Z0|Q581Z0_TRYB2 Histone-lysine N-methyltransferase, H3 lysine-79 specific OS=Trypanosoma brucei brucei (strai # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.4 0.0 8.1e-05 0.077 4 62 .. 131 198 .. 128 215 .. 0.71 Alignments for each domain: == domain 1 score: 11.4 bits; conditional E-value: 8.1e-05 EEEEET-TTSHHHHHHHHHSTTSEEEEEES-H...HHHHHHHHHHHHC.......TTSSEEEEES-CCC CS Methyltransf_4 4 vvleiGcGkGeflvalAkknPdknflGiEirv...kvvakvlkkieal.......elkNlrilrgdalk 62 ++ ++GcG+G++l + A ++GiEi++ kv +k+++ i+ + + ++i+ +d +k tr|Q581Z0|Q581Z0_TRYB2 131 TFYDLGCGNGSILFQVAFLTG-ARCVGIEISEhnaKVAKKAWEVIRPElegssgrSMPEVNIITSDMTK 198 789**************9875.6899***9872224455566655555666666555566666666555 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (173 nodes) Target sequences: 8561 (4332363 residues searched) Passed MSV filter: 234 (0.0273333); expected 171.2 (0.02) Passed bias filter: 189 (0.0220769); expected 171.2 (0.02) Passed Vit filter: 31 (0.00362107); expected 8.6 (0.001) Passed Fwd filter: 9 (0.00105128); expected 0.1 (1e-05) Initial search space (Z): 8561 [actual number of targets] Domain search space (domZ): 9 [number of targets reported over threshold] # CPU time: 0.08u 0.00s 00:00:00.08 Elapsed: 00:00:00.04 # Mc/sec: 18737.47 // [ok]