# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Methyltransf_5.hmm.txt # target sequence database: proteomes/Tetrahymena_thermophila.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Methyltransf_5 [M=309] Accession: PF01795.18 Description: MraW methylase family Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.9e-63 214.4 8.9 4.3e-63 214.3 1.8 2.5 2 tr|Q22Z09|Q22Z09_TETTS 16S rRNA methyltransferase OS=Tetrahy 6.6e-06 26.4 0.1 1.3e-05 25.4 0.0 1.5 2 tr|W7XCF3|W7XCF3_TETTS HemK methyltransferase family protein 0.0064 16.6 0.1 0.012 15.7 0.0 1.5 1 tr|Q239D4|Q239D4_TETTS Cyclopropane-fatty-acyl-phospholipid ------ inclusion threshold ------ 0.017 15.2 0.0 0.045 13.8 0.0 1.6 2 tr|W7X2L6|W7X2L6_TETTS 2-methoxy-6-polyprenyl-1,4-benzoquino 0.06 13.4 0.0 0.1 12.7 0.0 1.4 1 tr|I7MAE9|I7MAE9_TETTS UbiE/COQ5 family methyltransferase OS 1.4 8.9 5.3 2.7 8.0 2.4 2.0 2 tr|Q22KB0|Q22KB0_TETTS Anaphase-promoting complex subunit 11 Domain annotation for each sequence (and alignments): >> tr|Q22Z09|Q22Z09_TETTS 16S rRNA methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_0011 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 3.7 0.8 0.012 55 133 187 .. 17 68 .. 6 85 .. 0.82 2 ! 214.3 1.8 9.5e-67 4.3e-63 3 308 .. 99 410 .. 97 411 .. 0.88 Alignments for each domain: == domain 1 score: 3.7 bits; conditional E-value: 0.012 -HHHHHHH--HHHHHHHHHHHH--TTHHHHHHHHHHHHHHS---BHHHHHHHHHH CS Methyltransf_5 133 tAaevlnrlseeeLakilkeygeekfakriakaivekrkkkpiettkelaevikk 187 +++ln+ ++ L + +k+ g e+ +++k++++++k +++++++++ ++ tr|Q22Z09|Q22Z09_TETTS 17 QSSKLLNQQIQYSLFNFIKQEGLENKLSSLSKKVQQEKKGQKLSKVEQMI---QQ 68 46789*********************************999998877654...44 PP == domain 2 score: 214.3 bits; conditional E-value: 9.5e-67 XX--TTTHHHHHHT--........-TT-EEEES--TTSHHHHHHHTT--TTEEEEEES-HHHHHHHTT.....-.....--EEEEES CS Methyltransf_5 3 eHvpVLlqevvellep........kedgvyiDaTlGaggHsealLeqlselrligidrDksalarakerLkefe.....krltlvkg 76 H+pVL++++ ++l++ +++++++D+TlG++gH++++L++++ l ++g+d D+++l+ ++++k+ +r+++ ++ tr|Q22Z09|Q22Z09_TETTS 99 VHYPVLIDKITQVLQEefyphsssHSEKCLVDCTLGSAGHAKHFLQKFPYLYILGLDLDPRMLQYINDNVKSQLshsdqNRFKTRNE 185 7***********************9**************************************876655554332222355****** PP -GGGHHHHHHHTTTT.......-EEEEEEE-S--HHHHH-GGG---SSS--BS---S-TTSS-.-HHHHHHH--HHHHHHHHHHHH- CS Methyltransf_5 77 nfkelkealaeanvt.......kvdgillDLGvSslqldkaerGFsfaedapLDmRmdqsael.tAaevlnrlseeeLakilkeyge 155 nf++++++ + + ++ +i++DLG l+l+++e GFs+ +++ LDmR +qs++ +Aa++ln+ s+ eL +i+++yge tr|Q22Z09|Q22Z09_TETTS 186 NFTNIDKINLYKIFEeefpsykNFHAIFADLGYNTLHLENSEWGFSYLRNGKLDMRYNQSSQTnSAADILNKASYLELFEIIRQYGE 272 **99988655433223444444************************************998877*********************** PP -TTHHHHHHHHHHHHHHS---BHHHHHHHHHHHS-------...-TTHHHHHHHHHHHHTHHHHHHHHHHHHHHHEEEEEEEEEEES CS Methyltransf_5 156 ekfakriakaivekrkkkpiettkelaevikkvvpakekrk.kihpatrvFQAlRIavNdEleeleelleaalellakggrlavisF 241 + a i++ i ++r+ kp+ettk+++++i + + ++ + + + t+ FQAlRIa N+El +l+ +++++ + l k+g +i+F tr|Q22Z09|Q22Z09_TETTS 273 DPNADVISQMIIKQRQIKPFETTKDVVDLILEDQSGYSFKGgQYKAITKLFQALRIATNQELTNLQVFMDKIYDKLLKDGLGIIITF 359 ******************************99998888665256778**************************************** PP SHHHHHHHHHHHHHTT-------------------EEEEE.E.E---HHHHHH-GGGTT-EEEEEEE CS Methyltransf_5 242 hSledrivkkvfkeaskkeevPaglpvaseelkaklklitkkpilpseeeikeNprsrsakLrviek 308 hSle+ iv + +++++++ ++++++ p +e+++N +srsakL ++k tr|Q22Z09|Q22Z09_TETTS 360 HSLEENIVEQKLRDLKRQ---------------KNIEILQ-MGEKPDINELQKNSKSRSAKLYTFKK 410 ***************994...............3344444.3356999**************99987 PP >> tr|W7XCF3|W7XCF3_TETTS HemK methyltransferase family protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN= # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -3.8 0.0 2.3 1e+04 149 176 .. 47 74 .. 28 86 .. 0.68 2 ! 25.4 0.0 3e-09 1.3e-05 9 97 .. 103 196 .. 97 217 .. 0.88 Alignments for each domain: == domain 1 score: -3.8 bits; conditional E-value: 2.3 HHHHHH--TTHHHHHHHHHHHHHHS--- CS Methyltransf_5 149 ilkeygeekfakriakaivekrkkkpie 176 ++ + + + k+i k ++r+ pi+ tr|W7XCF3|W7XCF3_TETTS 47 MFSNCSSYYYLKQIQKMYFKRRAMMPIQ 74 4555555666677777777777666665 PP == domain 2 score: 25.4 bits; conditional E-value: 3e-09 THHHHHHT--.-TT-EEEES--TTSHHHHHHHTT--TTEEEEEES-HHHHHHHTT.....-...--EEEEES-G.GGHHHHHHHTTT CS Methyltransf_5 9 lqevvellep.kedgvyiDaTlGaggHsealLeqlselrligidrDksalarakerLkefe...krltlvkgnf.kelkealaeanv 90 l e+v+ l++ k++ ++ +G+g s lL++ s+ + i+id+ k ++ ak++L+ + +rl+l++ + k++++ l++++ tr|W7XCF3|W7XCF3_TETTS 103 LVELVNKLSKqKQKCQFLEIGIGTGAISLSLLKENSQFTGIAIDKQKFCIELAKQNLNLNKidpQRLQLIHLEClKFFQNNLNQNPD 189 4466777765377889****************************************9877655677*****9998**********9* PP T-EEEEE CS Methyltransf_5 91 tkvdgil 97 +++d+i+ tr|W7XCF3|W7XCF3_TETTS 190 QQFDFIV 196 ****997 PP >> tr|Q239D4|Q239D4_TETTS Cyclopropane-fatty-acyl-phospholipid synthase OS=Tetrahymena thermophila (strain SB210) OX=31 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 15.7 0.0 2.8e-06 0.012 24 86 .. 53 115 .. 25 136 .. 0.82 Alignments for each domain: == domain 1 score: 15.7 bits; conditional E-value: 2.8e-06 EEES--TTSHHHHHHHTT--TT.EEEEEES-HHHHHHHTT.....---EEEEES-GGGHHHHHH CS Methyltransf_5 24 yiDaTlGaggHsealLeqlsel.rligidrDksalarakerLkefekrltlvkgnfkelkeala 86 +D +G+g ++ ++++++ +++g+d+D++ +++a++ +k++++ +++ +nf el+++ + tr|Q239D4|Q239D4_TETTS 53 ILDIGIGTGLPLYKIASKFPQNaKILGVDIDENYVKAAQKLFKDKKN-VEIRLQNFYELDAKKE 115 57999999999999*******99*****************8888755.********99987655 PP >> tr|W7X2L6|W7X2L6_TETTS 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial OS=Tetrahymena thermophila (s # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.6 0.0 1 4.6e+03 43 63 .. 133 153 .. 100 175 .. 0.53 2 ? 13.8 0.0 1e-05 0.045 215 258 .. 192 235 .. 176 246 .. 0.85 Alignments for each domain: == domain 1 score: -2.6 bits; conditional E-value: 1 -TTEEEEEES-HHHHHHHTT. CS Methyltransf_5 43 selrligidrDksalaraker 63 ++l+++ +d+ +s+l++ k+r tr|W7X2L6|W7X2L6_TETTS 133 KNLKVTVLDINESMLEVGKRR 153 222444444555555444443 PP == domain 2 score: 13.8 bits; conditional E-value: 1e-05 HHHHHHHHHHHHHHHEEEEEEEEEEESSHHHHHHHHHHHHHTT- CS Methyltransf_5 215 EleeleelleaalellakggrlavisFhSledrivkkvfkeask 258 ++e++l++a ++l+kggrl+++ F +e+ v ++ k+++ tr|W7X2L6|W7X2L6_TETTS 192 NVPRIEKALSEAHRVLKKGGRLMILEFSKVEQFPVSEIYKQYNM 235 56789***************************999999998876 PP >> tr|I7MAE9|I7MAE9_TETTS UbiE/COQ5 family methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTH # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.7 0.0 2.3e-05 0.1 21 83 .. 52 114 .. 19 132 .. 0.80 Alignments for each domain: == domain 1 score: 12.7 bits; conditional E-value: 2.3e-05 T-EEEES--TTSHHHHHHHTT--TT.EEEEEES-HHHHHHHTT.....---EEEEES-GGGHHH CS Methyltransf_5 21 dgvyiDaTlGaggHsealLeqlsel.rligidrDksalarakerLkefekrltlvkgnfkelke 83 + +D+ +G+g +++++++ +++gid+D++ + +ak+ ++++ + +++ nf ++++ tr|I7MAE9|I7MAE9_TETTS 52 FKRILDVGVGTGHPLYSIIDRIPKSvQVTGIDIDTNYIPAAKKIFSKHSN-VEIKYMNFYDMEK 114 45678999999888899******999*****************9999976.7776666755544 PP >> tr|Q22KB0|Q22KB0_TETTS Anaphase-promoting complex subunit 11 RING-H2 finger protein OS=Tetrahymena thermophila (stra # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 8.0 2.4 0.00061 2.7 149 197 .. 20 68 .. 8 78 .. 0.77 2 ? 3.1 0.2 0.018 82 258 301 .. 74 119 .. 57 121 .. 0.66 Alignments for each domain: == domain 1 score: 8.0 bits; conditional E-value: 0.00061 HHHHHH--TTHHHHHHHHHHHHHHS---BHHHHHHHHHHHS-------. CS Methyltransf_5 149 ilkeygeekfakriakaivekrkkkpiettkelaevikkvvpakekrkk 197 l + +++++k i +i+e+rk k+i + ++++e+i + +k+ +k tr|Q22KB0|Q22KB0_TETTS 20 ALIKKQNIRYSKFIYINICEERKIKQIINSQQIQEFILQKRISKDLINK 68 45556799****************************9887666555554 PP == domain 2 score: 3.1 bits; conditional E-value: 0.018 -----..--------------EEEEE.E.E---HHHHHH-GGGTT- CS Methyltransf_5 258 kkeev..PaglpvaseelkaklklitkkpilpseeeikeNprsrsa 301 k+ ev + + + + +++ + +i k i+ + e ++N+ s sa tr|Q22KB0|Q22KB0_TETTS 74 KRFEVkkWNAVALWQWDIEVENCAICKNNIMEPCIECDANQNSNSA 119 333332244555666667778889*********************9 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (309 nodes) Target sequences: 26971 (16912921 residues searched) Passed MSV filter: 2464 (0.0913574); expected 539.4 (0.02) Passed bias filter: 252 (0.00934337); expected 539.4 (0.02) Passed Vit filter: 22 (0.000815691); expected 27.0 (0.001) Passed Fwd filter: 6 (0.000222461); expected 0.3 (1e-05) Initial search space (Z): 26971 [actual number of targets] Domain search space (domZ): 6 [number of targets reported over threshold] # CPU time: 0.36u 0.02s 00:00:00.38 Elapsed: 00:00:00.12 # Mc/sec: 43550.77 // [ok]