# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Methyltransf_5.hmm.txt # target sequence database: proteomes/Thermococcus_kodakarensis.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Methyltransf_5 [M=309] Accession: PF01795.18 Description: MraW methylase family Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 0.0063 13.1 0.0 0.085 9.4 0.0 2.1 2 tr|Q5JDP5|Q5JDP5_THEKO tRNA/rRNA cytosine-C5-methylase, NOL1 0.0075 12.9 0.0 0.024 11.2 0.0 1.8 2 tr|Q5JHQ2|Q5JHQ2_THEKO SAM-dependent methyltransferase, UbiE 0.0092 12.6 0.0 0.078 9.5 0.0 1.9 2 tr|Q5JFS6|Q5JFS6_THEKO Predicted SAM-dependent methyltransfe ------ inclusion threshold ------ 0.014 12.0 0.0 0.025 11.2 0.0 1.4 1 tr|Q5JEC3|Q5JEC3_THEKO Predicted DNA methylase OS=Thermococc Domain annotation for each sequence (and alignments): >> tr|Q5JDP5|Q5JDP5_THEKO tRNA/rRNA cytosine-C5-methylase, NOL1/NOP2/Sun family OS=Thermococcus kodakarensis (strain AT # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 9.4 0.0 0.00015 0.085 15 80 .. 245 312 .. 238 330 .. 0.81 2 ? 1.1 0.0 0.05 29 220 256 .. 360 399 .. 355 414 .. 0.70 Alignments for each domain: == domain 1 score: 9.4 bits; conditional E-value: 0.00015 HT---TT-EEEES--TTSHHHHHHHTT--TT.EEEEEES-HHHHHHHTT.....---.EEEEES-GGG CS Methyltransf_5 15 llepkedgvyiDaTlGaggHsealLeqlsel.rligidrDksalarakerLkefekr.ltlvkgnfke 80 +l+pk++ + +D gg + ++ e +++ +++++d D+ ++r ke+Lk+ + +++k+ ++ tr|Q5JDP5|Q5JDP5_THEKO 245 VLAPKPGETVVDLAAAPGGKTAHMAELMENRgKIYAFDVDSARIKRMKEVLKRTGVEiAEVIKADGRN 312 58899*********************999999********************9876634555555455 PP == domain 2 score: 1.1 bits; conditional E-value: 0.05 HHHHHHHHHHEEEEEEEEEEESSHH...HHHHHHHHHHHT CS Methyltransf_5 220 eelleaalellakggrlavisFhSl...edrivkkvfkea 256 +el+e+a +ll++ggrl + l ++ +vk++++ tr|Q5JDP5|Q5JDP5_THEKO 360 KELMESAWKLLKPGGRLLYSTCSMLpeeNEEVVKWFLERH 399 6899************975544333332456777777655 PP >> tr|Q5JHQ2|Q5JHQ2_THEKO SAM-dependent methyltransferase, UbiE/COQ5 family OS=Thermococcus kodakarensis (strain ATCC B # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 11.2 0.0 4.2e-05 0.024 16 82 .. 28 98 .. 21 115 .. 0.81 2 ? -1.3 0.0 0.28 1.6e+02 215 236 .. 117 138 .. 108 145 .. 0.79 Alignments for each domain: == domain 1 score: 11.2 bits; conditional E-value: 4.2e-05 T--......-TT-EEEES--TTSHHHHHHHTT--TTEEEEEES-HHHHHHHTT.....---EEEEES-GGGHH CS Methyltransf_5 16 lep......kedgvyiDaTlGaggHsealLeqlselrligidrDksalarakerLkefekrltlvkgnfkelk 82 lep k g +D G gg s lLe l +++g+d+ + +l++a+e+ ke+e r+++++g +el tr|Q5JHQ2|Q5JHQ2_THEKO 28 LEPllmkfmKTRGRVLDLACGVGGFS-FLLEDLGF-EVVGLDNSRFMLEKAREFAKEKESRVEFIEGDARELP 98 5555555668888999********98.67777765.799**************************99988876 PP == domain 2 score: -1.3 bits; conditional E-value: 0.28 HHHHHHHHHHHHHHHEEEEEEE CS Methyltransf_5 215 Eleeleelleaalellakggrl 236 E + l ++ +++ ++l++gg++ tr|Q5JHQ2|Q5JHQ2_THEKO 117 EPQDLAKVFKETARVLKPGGKF 138 6678889999999999999997 PP >> tr|Q5JFS6|Q5JFS6_THEKO Predicted SAM-dependent methyltransferase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 9.5 0.0 0.00014 0.078 10 81 .. 47 118 .. 44 136 .. 0.85 2 ? 1.0 0.0 0.054 31 221 242 .. 140 161 .. 136 195 .. 0.77 Alignments for each domain: == domain 1 score: 9.5 bits; conditional E-value: 0.00014 HHHHHHT---TT-EEEES--TTSHHHHHHHTT--TTEEEEEES-HHHHHHHTT.....---EEEEES-GGGH CS Methyltransf_5 10 qevvellepkedgvyiDaTlGaggHsealLeqlselrligidrDksalarakerLkefekrltlvkgnfkel 81 ++++e+++++ +g +D+ G+g+ + ++ + + ++g+dr +++++ +++ + +k+ ++v+gnf e+ tr|Q5JFS6|Q5JFS6_THEKO 47 KRLMEMITDEVKGKVLDVGCGTGVLTFKMALKNGVEMVVGVDRKEEVIEFCNRLGDRVTKNAKFVQGNFLEM 118 56789999999999***********999988899999**************9977888888********654 PP == domain 2 score: 1.0 bits; conditional E-value: 0.054 HHHHHHHHHEEEEEEEEEEESS CS Methyltransf_5 221 elleaalellakggrlavisFh 242 +le ale+l++ggr+ v F tr|Q5JFS6|Q5JFS6_THEKO 140 PFLERALEVLEEGGRVIVGDFD 161 5899************997775 PP >> tr|Q5JEC3|Q5JEC3_THEKO Predicted DNA methylase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.2 0.0 4.3e-05 0.025 11 98 .. 181 268 .. 175 315 .. 0.79 Alignments for each domain: == domain 1 score: 11.2 bits; conditional E-value: 4.3e-05 HHHHHT---TT-EEEES--TTSHHHHHHHTT--TTEEEEEES-HHHHHHHTT.....---EEEEES-GGGHHHHHHHTT..TT-EEE CS Methyltransf_5 11 evvellepkedgvyiDaTlGaggHsealLeqlselrligidrDksalarakerLkefekrltlvkgnfkelkealaean..vtkvdg 95 +++ +l++ +g ++D G g +++ l+ l+++g drD+ + ak++L+ k+++l ++ e+ +a + ++ +++d+ tr|Q5JEC3|Q5JEC3_THEKO 181 RIMVNLTEVRKGSFLDPFCGIGTVVQEFV--LQGLTAYGSDRDEGQIRDAKKNLTWLRKEFRLKNSAHLEVCDARKLKKcfRQRFDA 265 45666777888999999999999999987..456789************************99999886665555544444667787 PP EEE CS Methyltransf_5 96 ill 98 i+ tr|Q5JEC3|Q5JEC3_THEKO 266 IVT 268 776 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (309 nodes) Target sequences: 2301 (637680 residues searched) Passed MSV filter: 109 (0.0473707); expected 46.0 (0.02) Passed bias filter: 77 (0.0334637); expected 46.0 (0.02) Passed Vit filter: 12 (0.00521512); expected 2.3 (0.001) Passed Fwd filter: 4 (0.00173837); expected 0.0 (1e-05) Initial search space (Z): 2301 [actual number of targets] Domain search space (domZ): 4 [number of targets reported over threshold] # CPU time: 0.03u 0.00s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 9852.16 // [ok]