# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Methyltransf_8.hmm.txt # target sequence database: proteomes/Anolis_carolinensis.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Methyltransf_8 [M=219] Accession: PF05148.15 Description: Hypothetical methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.6e-06 28.2 0.0 2.3e-06 27.7 0.0 1.2 1 tr|G1KTN7|G1KTN7_ANOCA Uncharacterized protein OS=Anolis car 2.5e-05 24.3 0.0 3.6e-05 23.8 0.0 1.3 1 tr|G1KTN5|G1KTN5_ANOCA Uncharacterized protein OS=Anolis car 0.00036 20.5 0.0 0.0072 16.2 0.0 2.6 2 tr|H9G6W9|H9G6W9_ANOCA 2-methoxy-6-polyprenyl-1,4-benzoquino 0.00088 19.2 0.0 0.0023 17.8 0.0 1.6 1 tr|G1K8X1|G1K8X1_ANOCA NADH:ubiquinone oxidoreductase comple 0.0054 16.7 0.3 0.012 15.5 0.0 1.7 2 tr|R4GB35|R4GB35_ANOCA mRNA cap guanine-N7 methyltransferase ------ inclusion threshold ------ 0.022 14.7 0.1 0.15 11.9 0.0 2.1 2 tr|R4G9E3|R4G9E3_ANOCA Arsenite methyltransferase OS=Anolis Domain annotation for each sequence (and alignments): >> tr|G1KTN7|G1KTN7_ANOCA Uncharacterized protein OS=Anolis carolinensis OX=28377 GN=LOC100557412 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 27.7 0.0 7.3e-10 2.3e-06 118 173 .. 133 190 .. 118 217 .. 0.87 Alignments for each domain: == domain 1 score: 27.7 bits; conditional E-value: 7.3e-10 --TT-EEEEEEES---.SS-HHHHHHHHHHHEEEEEEEEEEE.EGGG-S-HHHHHHHH CS Methyltransf_8 118 ledesvdvavfclslm.gtnladflkeanrvlkvGGllkvae.vksrfesvklfaral 173 + d+svdv+v+ l l + n+++ lke+ r+lk+GG ++ e v s +s k f +++ tr|G1KTN7|G1KTN7_ANOCA 133 VSDGSVDVVVCTLVLCsVSNVTTVLKEVLRILKPGGAFFFMEhVASPRSSWKYFWQQV 190 579***********97368*****************9876654888888888887765 PP >> tr|G1KTN5|G1KTN5_ANOCA Uncharacterized protein OS=Anolis carolinensis OX=28377 GN=LOC100557610 PE=4 SV=2 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 23.8 0.0 1.2e-08 3.6e-05 111 158 .. 130 176 .. 105 194 .. 0.86 Alignments for each domain: == domain 1 score: 23.8 bits; conditional E-value: 1.2e-08 S-TTS-S--TT-EEEEEEES---.SS-HHHHHHHHHHHEEEEEEEEEEE CS Methyltransf_8 111 cdlakvPledesvdvavfclslm.gtnladflkeanrvlkvGGllkvae 158 dl ++P d svdv+v+ l l ++n a l e+ rvl++GG ++ e tr|G1KTN5|G1KTN5_ANOCA 130 EDLHQIP--DASVDVVVCTLVLCsVKNTARVLSEVLRVLRPGGAFYFME 176 4778887..99**********973689*****************99876 PP >> tr|H9G6W9|H9G6W9_ANOCA 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial OS=Anolis carolinensis OX=283 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.2 0.0 2.3e-06 0.0072 113 159 .. 183 230 .. 173 247 .. 0.84 2 ? 0.5 0.0 0.16 4.8e+02 156 187 .. 267 298 .. 260 305 .. 0.82 Alignments for each domain: == domain 1 score: 16.2 bits; conditional E-value: 2.3e-06 TTS-S--TT-EEEEEEES---.SS-HHHHHHHHHHHEEEEEEEEEEEE CS Methyltransf_8 113 lakvPledesvdvavfclslm.gtnladflkeanrvlkvGGllkvaev 159 ++P +d+ d+ + + + +t + + l+ea+rvlk+GG + e tr|H9G6W9|H9G6W9_ANOCA 183 AEELPFNDNNFDIYTIAFGIRnVTHIDQALQEAYRVLKPGGRFLCLEF 230 5689*********99999985389*****************9887765 PP == domain 2 score: 0.5 bits; conditional E-value: 0.16 EEEEGGG-S-HHHHHHHHHCTTEEEEEEE--S CS Methyltransf_8 156 vaevksrfesvklfaraltklGfevkskdlsn 187 + e rf+ + f++ ++ Gf ++ + + tr|H9G6W9|H9G6W9_ANOCA 267 LVESIRRFPAQEEFKEMIEDAGFLKVHYQNLS 298 5666779****************999987665 PP >> tr|G1K8X1|G1K8X1_ANOCA NADH:ubiquinone oxidoreductase complex assembly factor 5 OS=Anolis carolinensis OX=28377 GN=N # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 17.8 0.0 7.6e-07 0.0023 77 154 .. 78 168 .. 71 172 .. 0.70 Alignments for each domain: == domain 1 score: 17.8 bits; conditional E-value: 7.6e-07 EEES-TT-HHHHH--S-....EEEEESS......-SSTTE....EES-TTS-S--TT-EEEEEEES---.SS-HHHHHHHHHHHEEE CS Methyltransf_8 77 adlGcGeakiaeavrek..ekvrsfdlv......avnerv....vacdlakvPledesvdvavfclslm.gtnladflkeanrvlkv 150 dlGcG + ia++ ++ e++ d++ ++++++ v+ d +P ++++ d++v lsl +++l + l+e++rvlk+ tr|G1K8X1|G1K8X1_ANOCA 78 LDLGCGRSYIAQHLNKDiiERLFQADVAenalkkPIESEIprisVVADEEFLPFKENTFDLVVSGLSLHwVNDLPKALHEVHRVLKP 164 6999999999966554311444444543221111233332222356788899****************64899************** PP EEEE CS Methyltransf_8 151 GGll 154 G++ tr|G1K8X1|G1K8X1_ANOCA 165 DGVF 168 9986 PP >> tr|R4GB35|R4GB35_ANOCA mRNA cap guanine-N7 methyltransferase OS=Anolis carolinensis OX=28377 GN=RNMT PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 15.5 0.0 3.9e-06 0.012 60 111 .. 224 275 .. 203 292 .. 0.78 2 ? -4.0 0.0 3.7 1.2e+04 7 32 .. 365 390 .. 363 394 .. 0.78 Alignments for each domain: == domain 1 score: 15.5 bits; conditional E-value: 3.9e-06 HHHHHHHHCTS-TTS-EEEES-TT-H..HHHH--S-..EEEEESS-SSTTEEES CS Methyltransf_8 60 ldviirklkkkkdnlvvadlGcGeak..iaeavrekekvrsfdlvavnervvac 111 + +++ k++++k+n++v dlGcG+ + ++++ ++ + d++a+ v+ c tr|R4GB35|R4GB35_ANOCA 224 IGEFLDKVRRRKQNITVLDLGCGKGGdlLKWRKGRISRLVCTDIAAIS--VQQC 275 568999****************98642256899999999999999985..4445 PP == domain 2 score: -4.0 bits; conditional E-value: 3.7 -HHHHHHHHHHHHHHHHHS-HHHHHH CS Methyltransf_8 7 kkrldgarfrflneklytgesskavd 32 krl++++ + +++yt + +k+ + tr|R4GB35|R4GB35_ANOCA 365 VKRLEASQTDSFGNEIYTVKFEKKGQ 390 68999999999999999988877765 PP >> tr|R4G9E3|R4G9E3_ANOCA Arsenite methyltransferase OS=Anolis carolinensis OX=28377 GN=AS3MT PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 1.9 0.1 0.058 1.8e+02 74 106 .. 69 105 .. 32 112 .. 0.77 2 ? 11.9 0.0 5e-05 0.15 75 161 .. 94 185 .. 84 196 .. 0.78 Alignments for each domain: == domain 1 score: 1.9 bits; conditional E-value: 0.058 S-EEEES-TT-H....HHHH--S-..EEEEESS-SST CS Methyltransf_8 74 lvvadlGcGeak....iaeavrekekvrsfdlvavne 106 + dlGcG + +++ v+e+ ++ +d++ + tr|R4G9E3|R4G9E3_ANOCA 69 CWILDLGCGSGRdcymLSQLVGENGHITGIDMTEAQV 105 5699*****8773222336677777777777766555 PP == domain 2 score: 11.9 bits; conditional E-value: 5e-05 -EEEES-TT-HHHHH--S.-..EEEEESS-SSTTEEES-TTS-S...--TT-EEEEE.EES---SS-HHHHHHHHHHHEEEEEEEEE CS Methyltransf_8 75 vvadlGcGeakiaeavre.kekvrsfdlvavnervvacdlakvP...ledesvdvav.fclslmgtnladflkeanrvlkvGGllkv 156 + + ea++ a+++ +++ f + +n + + c + k+ l+ e ++++ c+ ++ + + l+ea+++lk GG ++ tr|R4G9E3|R4G9E3_ANOCA 94 HITGIDMTEAQVEVAKKHiDYHMNKFGYKKPNVNFIHCYMEKLSeagLKGECYNLVIsNCVINLSPDKRAVLHEAYQMLKAGGEMYF 180 455555666666644433145789999999*********98775222788999988835999999*********************9 PP EEEGG CS Methyltransf_8 157 aevks 161 ++v + tr|R4G9E3|R4G9E3_ANOCA 181 SDVYA 185 99865 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (219 nodes) Target sequences: 18528 (9796552 residues searched) Passed MSV filter: 445 (0.0240177); expected 370.6 (0.02) Passed bias filter: 300 (0.0161917); expected 370.6 (0.02) Passed Vit filter: 26 (0.00140328); expected 18.5 (0.001) Passed Fwd filter: 6 (0.000323834); expected 0.2 (1e-05) Initial search space (Z): 18528 [actual number of targets] Domain search space (domZ): 6 [number of targets reported over threshold] # CPU time: 0.15u 0.01s 00:00:00.16 Elapsed: 00:00:00.07 # Mc/sec: 30649.21 // [ok]