# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Methyltransf_8.hmm.txt # target sequence database: proteomes/Ciona_savignyi.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Methyltransf_8 [M=219] Accession: PF05148.15 Description: Hypothetical methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.9e-84 280.7 0.1 8.5e-84 280.2 0.1 1.2 1 tr|H2Z072|H2Z072_CIOSA Uncharacterized protein OS=Ciona savi 4.7e-07 29.3 0.5 2.9e-05 23.4 0.1 2.3 2 tr|H2YGP5|H2YGP5_CIOSA Uncharacterized protein OS=Ciona savi 1.1e-06 28.0 0.0 2e-06 27.2 0.0 1.4 1 tr|H2Y8E5|H2Y8E5_CIOSA Uncharacterized protein OS=Ciona savi 0.00017 20.9 0.1 0.053 12.7 0.0 2.3 2 tr|H2YK68|H2YK68_CIOSA S-adenosylmethionine sensor upstream 0.00053 19.3 0.0 0.00059 19.1 0.0 1.1 1 tr|H2Z5E6|H2Z5E6_CIOSA Uncharacterized protein OS=Ciona savi 0.0009 18.5 0.0 0.0013 18.0 0.0 1.2 1 tr|H2Z0R4|H2Z0R4_CIOSA Uncharacterized protein OS=Ciona savi 0.0011 18.3 0.0 0.01 15.1 0.0 2.0 1 tr|H2Z439|H2Z439_CIOSA Uncharacterized protein OS=Ciona savi ------ inclusion threshold ------ 0.043 13.0 0.0 0.26 10.5 0.0 2.0 1 tr|H2ZL66|H2ZL66_CIOSA Uncharacterized protein OS=Ciona savi 0.053 12.7 0.0 0.19 10.9 0.0 1.9 2 tr|H2Z0X8|H2Z0X8_CIOSA Uncharacterized protein OS=Ciona savi 0.054 12.7 0.0 0.15 11.3 0.0 1.6 2 tr|H2YJS4|H2YJS4_CIOSA Uncharacterized protein OS=Ciona savi Domain annotation for each sequence (and alignments): >> tr|H2Z072|H2Z072_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 280.2 0.1 7.3e-87 8.5e-84 2 219 .] 180 399 .] 179 399 .] 0.95 Alignments for each domain: == domain 1 score: 280.2 bits; conditional E-value: 7.3e-87 XXXXX-HHHHHHHHHHHHHHHHHS-HHHHHHHHHH-HHHHHHHHHHHHHHHCTSSS-HHHHHHHHHCTS-TTS-EEEES-TT-HHHH CS Methyltransf_8 2 leakikkrldgarfrflneklytgesskavdlfkedpeafdlyhkgfrsqvkkWPenPldviirklkkkkdnlvvadlGcGeakiae 88 l++++ ++l+++rfrf+ne++y+ +s +lf+ dp+af++yh+gf qv WP+nPld +ir++k++ +l++ad+GcGea +a+ tr|H2Z072|H2Z072_CIOSA 180 LKDRLVEKLESSRFRFINEQIYSQSSGDTHELFNSDPTAFQVYHRGFGAQVATWPVNPLDGVIRWVKERSPKLIIADFGCGEAMLAS 266 78999*********************************************************************************9 PP H--S-..EEEEESS-SSTTEEES-TTS-S--TT-EEEEEEES---SS-HHHHHHHHHHHEEEEEEEEEEEEGGG-S-HHHHHHHHHC CS Methyltransf_8 89 avrekekvrsfdlvavnervvacdlakvPledesvdvavfclslmgtnladflkeanrvlkvGGllkvaevksrfesvklfaraltk 175 +v++k v+sfdlvavn+rv++ d+akvPl+d+svd++vfclslmgtnl +fl eanrvlk G+lk+aev srf+ k+f r+l+k tr|H2Z072|H2Z072_CIOSA 267 SVKNK--VHSFDLVAVNDRVTVADIAKVPLADSSVDIVVFCLSLMGTNLVEFLVEANRVLKLRGVLKIAEVASRFSGLKQFYRDLKK 351 99887..******************************************************************************** PP TTEEEEEEE--STTEEEEEEEE-SSS-TT...---.GG--------XX CS Methyltransf_8 176 lGfevkskdlsnkqfvlfefkkvekkgkk...rel.glklkpclykkr 219 +Gf+ ++kd +n++f++f+f k+++ + +++ glklk+c ykkr tr|H2Z072|H2Z072_CIOSA 352 IGFDLHKKDTTNSHFFMFDFFKTKNSPPSclsNDYsGLKLKACAYKKR 399 ***********************97665312234459**********9 PP >> tr|H2YGP5|H2YGP5_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 4.2 0.1 0.019 22 74 103 .. 32 65 .. 27 72 .. 0.78 2 ! 23.4 0.1 2.5e-08 2.9e-05 99 161 .. 82 146 .. 61 158 .. 0.77 Alignments for each domain: == domain 1 score: 4.2 bits; conditional E-value: 0.019 S-EEEES-TT-H....HHHH--S-..EEEEESS- CS Methyltransf_8 74 lvvadlGcGeak....iaeavrekekvrsfdlva 103 l v+dlGcG + ia+ v+e+ +v+ +d++ tr|H2YGP5|H2YGP5_CIOSA 32 LKVVDLGCGSGRdcyaIAQLVGENGEVIGVDMTK 65 5699*****9875555888889999999998865 PP == domain 2 score: 23.4 bits; conditional E-value: 2.5e-08 EESS-SSTTEEES-TTS-S.--TT-EEEEEE.ES---SS-HHHHHHHHHHHEEEEEEEEEEEEGG CS Methyltransf_8 99 fdlvavnervvacdlakvP.ledesvdvavf.clslmgtnladflkeanrvlkvGGllkvaevks 161 f ++ n r v + + ++d+svd+a+ c+ + +n + ke+ r+lk+GG ++ +++ s tr|H2YGP5|H2YGP5_CIOSA 82 FGFKESNVRFVEGYIEDLSfIADSSVDIAISnCVVNLCENKEAVFKEVFRILKTGGEFYWSDIYS 146 5555566666666665554269*******955**************************9999977 PP >> tr|H2Y8E5|H2Y8E5_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 27.2 0.0 1.7e-09 2e-06 117 162 .. 143 189 .. 99 202 .. 0.81 Alignments for each domain: == domain 1 score: 27.2 bits; conditional E-value: 1.7e-09 S--TT-EEEEEE.ES---SS-HHHHHHHHHHHEEEEEEEEEEEEGGG CS Methyltransf_8 117 Pledesvdvavf.clslmgtnladflkeanrvlkvGGllkvaevksr 162 ++desvd+++ c+ ++++ + lkea+rvlk GG l+ ++v s tr|H2Y8E5|H2Y8E5_CIOSA 143 GIADESVDIIISnCVINLVKDKEAVLKEAYRVLKYGGELYFSDVYSD 189 589******99549999**************************9985 PP >> tr|H2YK68|H2YK68_CIOSA S-adenosylmethionine sensor upstream of mTORC1 OS=Ciona savignyi OX=51511 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 12.7 0.0 4.6e-05 0.053 57 121 .. 108 171 .. 94 186 .. 0.82 2 ! 6.1 0.0 0.0049 5.7 122 200 .. 195 281 .. 174 294 .. 0.68 Alignments for each domain: == domain 1 score: 12.7 bits; conditional E-value: 4.6e-05 S-HHHHHHHHHCTS-TTS-EEEES-TT-HHHHH--S-..EEEEESS-SSTTEEES-TTS-S--TT CS Methyltransf_8 57 enPldviirklkkkkdnlvvadlGcGeakiaeavrekekvrsfdlvavnervvacdlakvPlede 121 +nP d+++++ k+ ++n ++ l G + ++ ++ + ++dl + n+ v cd+ v + d tr|H2YK68|H2YK68_CIOSA 108 KNPCDKVLKS-KSPQQNGSIRLLDVGSCYNPFSEMQEFETTAIDLSPANDTVYQCDFISVEVSDV 171 5788887765.56667778887888888888888888899*****************99998885 PP == domain 2 score: 6.1 bits; conditional E-value: 0.0049 -EEEEEEES---S....S-HHHHHHHHHHHEEEEEEEEEEEEGGG-S.....-HHHHHHHHHCTTEEEEEEE--STTEEEEEEEE-S CS Methyltransf_8 122 svdvavfclslmg....tnladflkeanrvlkvGGllkvaevksrfe.....svklfaraltklGfevkskdlsnkqfvlfefkkve 199 dv+vf l l + ++ a++vl + G+l + s + ++k+++ ++++Gf+ + +++ ++ l+ f+k++ tr|H2YK68|H2YK68_CIOSA 195 HYDVVVFSLLLSYipcpQARWEMCVKAHQVLSTNGILIIITPDSCHQnkhasNMKKWKLSIEEIGFQRIKY-EKDVHLHLMAFRKTP 280 457777766554311112223445568999*99999999888776554444478889999******87654.5678999******98 PP S CS Methyltransf_8 200 k 200 tr|H2YK68|H2YK68_CIOSA 281 F 281 4 PP >> tr|H2Z5E6|H2Z5E6_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 19.1 0.0 5.1e-07 0.00059 114 161 .. 23 71 .. 14 107 .. 0.78 Alignments for each domain: == domain 1 score: 19.1 bits; conditional E-value: 5.1e-07 TS-S--TT-EEEEEEES---SS-HH.HHHHHHHHHEEEEEEEEEEEEGG CS Methyltransf_8 114 akvPledesvdvavfclslmgtnla.dflkeanrvlkvGGllkvaevks 161 +++P++d s dv++ + ++ d +k++ r+lk+GG+++ + ks tr|H2Z5E6|H2Z5E6_CIOSA 23 NRIPVDDASYDVIISIGGFSPSHIQaDCIKDVVRLLKPGGIFWFSIRKS 71 68*************9999999986479**************9875554 PP >> tr|H2Z0R4|H2Z0R4_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 18.0 0.0 1.1e-06 0.0013 115 199 .. 117 206 .. 108 216 .. 0.67 Alignments for each domain: == domain 1 score: 18.0 bits; conditional E-value: 1.1e-06 S-S--TT-EEEEEEES---SS-HH.HHHHHHHHHEEEEEEEEEEEEGGG-S..-HHHHHHHHHCTTEEEEEEE--S..TTEEEEEEE CS Methyltransf_8 115 kvPledesvdvavfclslmgtnla.dflkeanrvlkvGGllkvaevksrfe..svklfaraltklGfevkskdlsn..kqfvlfefk 196 +Pl+d+ dv+ + s+ + ++ + lke rv k+GG++ a v++ + +++a+ +++l + k+l+ + + ++f tr|H2Z0R4|H2Z0R4_CIOSA 117 ALPLKDQMYDVTCCLGSMSVPHIQpEALKEFVRVTKTGGMMGFAVVTTPRSgaYEQKLAEIVKSLENSGQWKNLKAikSAIFQYNFD 203 68********98666688888888568*****************9987444332334555555554444444433300333344555 PP E-S CS Methyltransf_8 197 kve 199 k++ tr|H2Z0R4|H2Z0R4_CIOSA 204 KID 206 555 PP >> tr|H2Z439|H2Z439_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 15.1 0.0 8.6e-06 0.01 59 176 .. 15 151 .. 7 171 .. 0.66 Alignments for each domain: == domain 1 score: 15.1 bits; conditional E-value: 8.6e-06 HHHHHHHHHCTS-TTS-EEEES-TT-HHHHH--S-..EE....EEE....S..S-SSTTE....EES....-TTS-S--TT-EEEEE CS Methyltransf_8 59 PldviirklkkkkdnlvvadlGcGeakiaeavrekekvr....sfd....l..vavnerv....vac....dlakvPledesvdvav 127 d++++++k + + v dlG G +++ r++ + + +d + +a +++v + c d +Pl d + dv+v tr|H2Z439|H2Z439_CIOSA 15 ITDMFLKHVKDRTNT-KVLDLGGGTGSVSQLLRNQLQFKgeidILDanmdMlyKASKKEVgfsnILChwigDSGDLPLRDATYDVIV 100 568889999988765.56799999999998888877654211133322222223445555333345644446679************ PP EES---SS-HHHH.HHHHHHHEEEEEEEEEE.EEGGG-S-HHHHHHHHHCT CS Methyltransf_8 128 fclslmgtnladf.lkeanrvlkvGGllkva.evksrfesvklfaraltkl 176 + +++ + + + +k + +v+k+ Gll ++ +++ + k+++ ++++l tr|H2Z439|H2Z439_CIOSA 101 CAAAFLPNHIPAPaIKGILKVIKPEGLLLLTmRLETTGSYEKVLQDNIKQL 151 *********976539***********9986412333333344455555555 PP >> tr|H2ZL66|H2ZL66_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.5 0.0 0.00023 0.26 71 154 .. 70 167 .. 54 178 .. 0.69 Alignments for each domain: == domain 1 score: 10.5 bits; conditional E-value: 0.00023 -TTS-EEEES-TT-HHHHH--S-..EEEE...E......SS-SSTTEEES-T....TS-S--TT-EEEEEEES---.SS-HHHHHHH CS Methyltransf_8 71 kdnlvvadlGcGeakiaeavrekekvrsf...d......lvavnervvacdl....akvPledesvdvavfclslm.gtnladflke 143 k++ dlGcG i+++ +e++ + + d + ++++++ + +P ++ d+++ +sl +++l + ke tr|H2ZL66|H2ZL66_CIOSA 70 KSSPLALDLGCGRGYISQELSEFDGIDLLfqgDtsqsylNNPCQPKLTTHSVnfneESLPFRTDTFDMVLSSMSLHwVNDLPKCFKE 156 45555678888888888888877666433122111111023333443332222133689***************964889******* PP HHHHEEEEEEE CS Methyltransf_8 144 anrvlkvGGll 154 + r+lk+ G + tr|H2ZL66|H2ZL66_CIOSA 157 VLRILKPDGCF 167 ******99975 PP >> tr|H2Z0X8|H2Z0X8_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.9 0.0 0.00017 0.19 33 73 .. 16 56 .. 12 98 .. 0.80 2 ? -2.2 0.0 1.7 1.9e+03 37 59 .. 181 198 .. 175 201 .. 0.80 Alignments for each domain: == domain 1 score: 10.9 bits; conditional E-value: 0.00017 HHHH-HHHHHHHHHHHHHHHCTSSS-HHHHHHHHHCTS-TT CS Methyltransf_8 33 lfkedpeafdlyhkgfrsqvkkWPenPldviirklkkkkdn 73 ++ e+ +fd+y ++ + + + P++P++ +i +lk+ ++ tr|H2Z0X8|H2Z0X8_CIOSA 16 VYAEEHGIFDMYKRLLCQLIINKPTEPIQYVIDWLKRDNNP 56 6789999****************************987664 PP == domain 2 score: -2.2 bits; conditional E-value: 1.7 -HHHHHHHHHHHHHHHCTSSS-H CS Methyltransf_8 37 dpeafdlyhkgfrsqvkkWPenP 59 dp+ d+yh f+ WP++P tr|H2Z0X8|H2Z0X8_CIOSA 181 DPTTGDVYHTTFD-----WPQDP 198 89999****9995.....66666 PP >> tr|H2YJS4|H2YJS4_CIOSA Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -1.8 0.0 1.3 1.5e+03 73 93 .. 80 100 .. 59 113 .. 0.78 2 ? 11.3 0.0 0.00013 0.15 115 156 .. 138 180 .. 125 200 .. 0.86 Alignments for each domain: == domain 1 score: -1.8 bits; conditional E-value: 1.3 TS-EEEES-TT-HHHHH--S- CS Methyltransf_8 73 nlvvadlGcGeakiaeavrek 93 + + d+G G ++a a ++ tr|H2YJS4|H2YJS4_CIOSA 80 SFTALDIGAGTGQMAIALKNI 100 556689999999999776654 PP == domain 2 score: 11.3 bits; conditional E-value: 0.00013 S-S--TT-EEEEEEES---SS-HH.HHHHHHHHHEEEEEEEEE CS Methyltransf_8 115 kvPledesvdvavfclslmgtnla.dflkeanrvlkvGGllkv 156 +P e + d +v+c +++ ++ + l ++ rv+k+GG l+ tr|H2YJS4|H2YJS4_CIOSA 138 PLPYESNKYDAVVCCCAMVPALIQpECLPDMARVVKPGGQLYF 180 5899999**********999999856899***********996 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (219 nodes) Target sequences: 11592 (4231397 residues searched) Passed MSV filter: 283 (0.0244134); expected 231.8 (0.02) Passed bias filter: 200 (0.0172533); expected 231.8 (0.02) Passed Vit filter: 27 (0.00232919); expected 11.6 (0.001) Passed Fwd filter: 10 (0.000862664); expected 0.1 (1e-05) Initial search space (Z): 11592 [actual number of targets] Domain search space (domZ): 10 [number of targets reported over threshold] # CPU time: 0.09u 0.00s 00:00:00.09 Elapsed: 00:00:00.03 # Mc/sec: 30889.20 // [ok]