# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Methyltransf_8.hmm.txt # target sequence database: proteomes/Dictyostelium_discoideum.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Methyltransf_8 [M=219] Accession: PF05148.15 Description: Hypothetical methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.7e-80 268.9 6.2 2.7e-80 268.9 6.2 2.5 2 sp|Q54CP1|RRP8_DICDI Ribosomal RNA-processing protein 8 OS 3.2e-06 26.7 0.1 0.00023 20.6 0.1 2.2 1 tr|Q54GQ2|Q54GQ2_DICDI Uncharacterized protein OS=Dictyostel 6.3e-06 25.7 0.0 1.3e-05 24.7 0.0 1.6 1 tr|Q54ET9|Q54ET9_DICDI Uncharacterized protein OS=Dictyostel 7.2e-06 25.5 0.0 0.00025 20.5 0.0 2.2 1 tr|Q55GC0|Q55GC0_DICDI Uncharacterized protein OS=Dictyostel 1.4e-05 24.6 0.0 2.2e-05 23.9 0.0 1.3 1 tr|Q54EN8|Q54EN8_DICDI Uncharacterized protein OS=Dictyostel 7e-05 22.3 0.0 0.00011 21.6 0.0 1.3 1 tr|Q54EP8|Q54EP8_DICDI Uncharacterized protein OS=Dictyostel 7.4e-05 22.2 0.0 0.0011 18.4 0.0 2.5 2 tr|Q553T0|Q553T0_DICDI Uncharacterized protein OS=Dictyostel 0.00097 18.6 0.0 0.012 15.0 0.0 2.2 2 sp|Q54VN2|COQ5_DICDI 2-methoxy-6-polyprenyl-1,4-benzoquino 0.0017 17.7 0.0 0.31 10.3 0.0 2.1 2 tr|Q54SL7|Q54SL7_DICDI Uncharacterized protein OS=Dictyostel 0.0018 17.7 0.7 0.0048 16.3 0.7 1.8 1 tr|Q54LU3|Q54LU3_DICDI Uncharacterized protein OS=Dictyostel ------ inclusion threshold ------ 0.052 12.9 0.0 0.15 11.4 0.0 1.7 2 tr|Q54GQ3|Q54GQ3_DICDI Uncharacterized protein OS=Dictyostel Domain annotation for each sequence (and alignments): >> sp|Q54CP1|RRP8_DICDI Ribosomal RNA-processing protein 8 OS=Dictyostelium discoideum OX=44689 GN=rrp8 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.0 0.3 1.6 1.9e+03 61 102 .. 68 110 .. 58 124 .. 0.67 2 ! 268.9 6.2 2.3e-83 2.7e-80 1 219 [] 157 390 .] 157 390 .] 0.91 Alignments for each domain: == domain 1 score: -2.0 bits; conditional E-value: 1.6 HHHHHHHCTS-TTS-EEEES-TT-H.HHHH--S-..EEEEESS CS Methyltransf_8 61 dviirklkkkkdnlvvadlGcGeak.iaeavrekekvrsfdlv 102 ++ ++lkk ++n+++ + + k i ++ +++ekv+s d+ sp|Q54CP1|RRP8_DICDI 68 NIKNKNLKKVENNKSLKVISSNKNKnILNNLKKEEKVNSNDIL 110 5555677777777777777766555255777777777777764 PP == domain 2 score: 268.9 bits; conditional E-value: 2.3e-83 XXXXXX-HHHHHHHHHHHHHHHHHS-HHHHHHHHHH-HHHHHHHHHHHHHHHCTSSS-HHHHHHHHHCTS-TTS-EEEES-TT-HHHHH CS Methyltransf_8 1 dleakikkrldgarfrflneklytgesskavdlfkedpeafdlyhkgfrsqvkkWPenPldviirklkkkkdnlvvadlGcGeakiaea 89 dl+++++++l g+rfr+lne lyt++s++a++ f ed ++fd yh gf+sqv+ WP nPld ii +l + k+ + +adlGcGeak+ae+ sp|Q54CP1|RRP8_DICDI 157 DLQNEMSEKLKGSRFRWLNETLYTTHSKEAFKEFSEDRSLFDQYHSGFKSQVESWPINPLDLIIDDLSSIKQRKRIADLGCGEAKLAER 245 699************************************************************************************** PP --S-..EEEEESS-SSTTEEES-TTS-S--TT-EEEEEEES---SS-HHHHHHHHHHHEEEEEEEEEEEEGGG-S-HHHHHHHHHCTTE CS Methyltransf_8 90 vrekekvrsfdlvavnervvacdlakvPledesvdvavfclslmgtnladflkeanrvlkvGGllkvaevksrfesvklfaraltklGf 178 ++k+ ++sfdlvavnerv+acd++++Pl++es+d+avfclslmgtn+ df+ ea+rvl +GGllk+ae++sr+++++ f++++++ Gf sp|Q54CP1|RRP8_DICDI 246 LQHKHTIQSFDLVAVNERVTACDISNLPLKNESIDIAVFCLSLMGTNFIDFIIEAERVLVKGGLLKIAEIESRITDINAFTNEIQQHGF 334 ***************************************************************************************** PP EEEEEE--STTEEEEEEEE-S.............SS-TT---..GG--------XX CS Methyltransf_8 179 evkskdlsnkqfvlfefkkve.............kkgkkrel..glklkpclykkr 219 + +k+++n++f+lfef k + +k kk++ ++ lkpclykkr sp|Q54CP1|RRP8_DICDI 335 NLIKKNEQNQYFTLFEFSKLQkkdqqfmrslkqyQKLKKQQAtnEPVLKPCLYKKR 390 *****************999833333333322221112222223567889999987 PP >> tr|Q54GQ2|Q54GQ2_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0188678 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 20.6 0.1 2e-07 0.00023 51 156 .. 23 150 .. 8 192 .. 0.67 Alignments for each domain: == domain 1 score: 20.6 bits; conditional E-value: 2e-07 HHCTSSS-HHHHHHHHHC...TS-TTS-EEEES-TT-HHHHH-..-S-..EEEEE............SS....-SSTT..EEES-TT CS Methyltransf_8 51 qvkkWPenPldviirklk...kkkdnlvvadlGcGeakiaeav..rekekvrsfd............lv....avner..vvacdla 114 vk+ P P++ i+ lk + ++dl cG k ++ + ++++v +++ l+ n++ vv + tr|Q54GQ2|Q54GQ2_DICDI 23 YVKGRPSYPIEA-IKMLKedlGLTIDSKIMDLACGTGKFSKMIceADFKNVVCVEpspefrvdcsyvLKefidDKNKDiqVVNGLAT 108 567777777765.35555222223355677777777777754411345566654311111111111111111122344114444458 PP S-S--TT-EEEEEEES---SS-HHHHHHHHHHHEEEEEEEEE CS Methyltransf_8 115 kvPledesvdvavfclslmgtnladflkeanrvlkvGGllkv 156 +P e+++ d + s+ + d +ke+ rvlk+GG+l++ tr|Q54GQ2|Q54GQ2_DICDI 109 SLPFENSTFDCICAAQSFHWYDNVDAIKEITRVLKPGGVLYL 150 9**************************************996 PP >> tr|Q54ET9|Q54ET9_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0219665 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 24.7 0.0 1.1e-08 1.3e-05 98 169 .. 103 174 .. 67 216 .. 0.74 Alignments for each domain: == domain 1 score: 24.7 bits; conditional E-value: 1.1e-08 EEESS-SSTTEEES-TTS-S--TT-EE..EEEEES---SS-HHHHHHHHHHHEEEEEEEEEEEEGGG-S-HHHH CS Methyltransf_8 98 sfdlvavnervvacdlakvPledesvd..vavfclslmgtnladflkeanrvlkvGGllkvaevksrfesvklf 169 s ++ +n +++ c+ ++P e++s d fcl l +t +++lkea+rvl vGG+ v r e+ ++f tr|Q54ET9|Q54ET9_DICDI 103 SNEISELNFKIQQCNAEQLPFENGSFDryFSNFCLHL-VTSPETMLKEAYRVLEVGGIAAF-SVWGRPENSNQF 174 6677889999*****************555779**98.577899*************9765.355555544443 PP >> tr|Q55GC0|Q55GC0_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0189501 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 20.5 0.0 2.2e-07 0.00025 95 156 .. 92 153 .. 8 169 .. 0.89 Alignments for each domain: == domain 1 score: 20.5 bits; conditional E-value: 2.2e-07 .EEEEESS-SSTTEEES-TTS-S--TT-EEEEEEES---SS-HHHHHHHHHHHEEEEEEEEE CS Methyltransf_8 95 kvrsfdlvavnervvacdlakvPledesvdvavfclslmgtnladflkeanrvlkvGGllkv 156 kv+ + ++n +v + +Pl desvdv + s + + l e rvlkvGG+l + tr|Q55GC0|Q55GC0_DICDI 92 KVKNEESPSLNYHVLDGISTSIPLPDESVDVLFISQSYHWFDNVQSLTEFSRVLKVGGVLVM 153 555666667788888877799*****************99999999************9865 PP >> tr|Q54EN8|Q54EN8_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0183885 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 23.9 0.0 1.9e-08 2.2e-05 105 190 .. 112 198 .. 107 216 .. 0.75 Alignments for each domain: == domain 1 score: 23.9 bits; conditional E-value: 1.9e-08 STTEEES-TTS-S--TT-EE..EEEEES---SS-HHHHHHHHHHHEEEEEEEEEEEEGGG-S-HHHH..HHHH.HCTTEEEEEEE-- CS Methyltransf_8 105 nervvacdlakvPledesvd..vavfclslmgtnladflkeanrvlkvGGllkvaevksrfesvklf..aral.tklGfevkskdls 186 n +++ c+ k+P d+s d +cl l+ t +++lkea+rvl vGG+ v r e+ ++f ++++ +++G +++s++ s tr|Q54EN8|Q54EN8_DICDI 112 NFKIQQCNAEKLPFPDNSFDryFSNYCLHLV-TSPETMLKEAYRVLEVGGIAAF-SVWGRPENSNQFtiVKKIaDEIGIDMSSQERS 196 567899**************555779**985.77899*************9765.57788776665522344425788888877654 PP STTE CS Methyltransf_8 187 nkqf 190 f tr|Q54EN8|Q54EN8_DICDI 197 --SF 198 ..34 PP >> tr|Q54EP8|Q54EP8_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0183875 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 21.6 0.0 9.7e-08 0.00011 105 156 .. 108 160 .. 98 204 .. 0.79 Alignments for each domain: == domain 1 score: 21.6 bits; conditional E-value: 9.7e-08 STTEEES-TTS-S--TT-EE..EEEEES---SS-HHHHHHHHHHHEEEEEEEEE CS Methyltransf_8 105 nervvacdlakvPledesvd..vavfclslmgtnladflkeanrvlkvGGllkv 156 n +++ c+ k+P d+s d +cl l+ t +++lkea+rvl vGG+ tr|Q54EP8|Q54EP8_DICDI 108 NFKIQQCNAEKLPFPDNSFDryFSNYCLHLV-TSPETMLKEAYRVLEVGGIAAF 160 567999**************555779**985.77899*************8654 PP >> tr|Q553T0|Q553T0_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0202574 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -0.2 0.0 0.45 5.2e+02 133 158 .. 136 161 .. 134 180 .. 0.86 2 ! 18.4 0.0 9.1e-07 0.0011 108 154 .. 293 340 .. 200 346 .. 0.78 Alignments for each domain: == domain 1 score: -0.2 bits; conditional E-value: 0.45 -SS-HHHHHHHHHHHEEEEEEEEEEE CS Methyltransf_8 133 mgtnladflkeanrvlkvGGllkvae 158 m+ ++ fl+ +++ + +G ++k+a tr|Q553T0|Q553T0_DICDI 136 MTHTWNAFLESIKKTVETGSIVKIAP 161 78899******************995 PP == domain 2 score: 18.4 bits; conditional E-value: 9.1e-07 EEES-TTS-S--TT-EEEEEEES---.SS-HHHHHHHHHHHEEEEEEE CS Methyltransf_8 108 vvacdlakvPledesvdvavfclslm.gtnladflkeanrvlkvGGll 154 d k+P d++ dv+ f s + + f+ke +rvlk+GG + tr|Q553T0|Q553T0_DICDI 293 FDHGDAMKMPYPDNTFDVVTFFESTChMPDKQAFIKECYRVLKPGGRM 340 4567999*************99987515699***************87 PP >> sp|Q54VN2|COQ5_DICDI 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial OS=Dictyostelium discoideum OX= # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 15.0 0.0 1e-05 0.012 114 158 .. 188 233 .. 159 244 .. 0.85 2 ? 0.9 0.0 0.21 2.4e+02 157 184 .. 272 299 .. 266 307 .. 0.81 Alignments for each domain: == domain 1 score: 15.0 bits; conditional E-value: 1e-05 TS-S--TT-EEEEEEES---.SS-HHHHHHHHHHHEEEEEEEEEEE CS Methyltransf_8 114 akvPledesvdvavfclslm.gtnladflkeanrvlkvGGllkvae 158 ++P +d++ + + + + tn+ + l+ea+rvlk+GG + e sp|Q54VN2|COQ5_DICDI 188 EQLPFKDNTFNCYTVSFGIRnCTNIDQVLREAYRVLKPGGRFLCLE 233 68999999998877777774279*****************987666 PP == domain 2 score: 0.9 bits; conditional E-value: 0.21 EEEGGG-S-HHHHHHHHHCTTEEEEEEE CS Methyltransf_8 157 aevksrfesvklfaraltklGfevkskd 184 e +f++ ++f++ ++ Gf+++ + sp|Q54VN2|COQ5_DICDI 272 VESIRKFPDQETFVQMIQDAGFKQVTYK 299 55566899**************997654 PP >> tr|Q54SL7|Q54SL7_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0204207 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 5.5 0.0 0.0084 9.7 60 97 .. 32 67 .. 22 80 .. 0.70 2 ! 10.3 0.0 0.00027 0.31 126 159 .. 121 154 .. 113 212 .. 0.76 Alignments for each domain: == domain 1 score: 5.5 bits; conditional E-value: 0.0084 HHHHHHHHCTS-TTS-EEEES-TT-HHHHH--S-..EE CS Methyltransf_8 60 ldviirklkkkkdnlvvadlGcGeakiaeavrekekvr 97 l+v+++k+ kkkd+ + +GcG++k+ e +++ e v tr|Q54SL7|Q54SL7_DICDI 32 LKVFLNKFFKKKDK--ILMIGCGNSKLGEDMNDDEFVD 67 56667777777776..5669*******98776654443 PP == domain 2 score: 10.3 bits; conditional E-value: 0.00027 EEEES---SS-HHHHHHHHHHHEEEEEEEEEEEE CS Methyltransf_8 126 avfclslmgtnladflkeanrvlkvGGllkvaev 159 av+c +n ++ l e+ rvlk+GG++ v tr|Q54SL7|Q54SL7_DICDI 121 AVMCSDDDNENAKQILLEVSRVLKPGGFFIVMTY 154 7888888999******************998654 PP >> tr|Q54LU3|Q54LU3_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0186961 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.3 0.7 4.2e-06 0.0048 104 171 .. 126 198 .. 57 241 .. 0.79 Alignments for each domain: == domain 1 score: 16.3 bits; conditional E-value: 4.2e-06 SSTTEEES-TTS-S--TT-EEE.EEEES---SS..-HHHHHHHHHHHEEEEEEEEEEEE..GGG-S-HHHHHH CS Methyltransf_8 104 vnervvacdlakvPledesvdv.avfclslmgt..nladflkeanrvlkvGGllkvaev..ksrfesvklfar 171 +n ++ d +++P++d+ d ++ c l+ n + e+ rv+k GG + +++ +s +s+ + ++ tr|Q54LU3|Q54LU3_DICDI 126 PNVKIFIGDASRIPIDDNYFDAyVIRCERLLQHviNPDLVISEMTRVIKSGGRIVITDAdwSSTSVSIPKNIE 198 57788889*************73567999987611556789************99976511444444444444 PP >> tr|Q54GQ3|Q54GQ3_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0188677 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.7 0.0 2.7 3.1e+03 64 87 .. 39 61 .. 23 78 .. 0.65 2 ? 11.4 0.0 0.00013 0.15 104 156 .. 101 153 .. 79 165 .. 0.79 Alignments for each domain: == domain 1 score: -2.7 bits; conditional E-value: 2.7 HHHHCTS-TTS-EEEES-TT-HHH CS Methyltransf_8 64 irklkkkkdnlvvadlGcGeakia 87 i++l ++ v++dl G k + tr|Q54GQ3|Q54GQ3_DICDI 39 IKSLG-LSNDSVIVDLASGPGKFT 61 34443.356789999999988766 PP == domain 2 score: 11.4 bits; conditional E-value: 0.00013 SSTTEEES-TTS-S--TT-EEEEEEES---SS-HHHHHHHHHHHEEEEEEEEE CS Methyltransf_8 104 vnervvacdlakvPledesvdvavfclslmgtnladflkeanrvlkvGGllkv 156 +n +v + +Pl d+svd + + + + ++e+ rvlk+ G+l + tr|Q54GQ3|Q54GQ3_DICDI 101 LNFKVLEGLSTSIPLPDNSVDCITVATAWHWFSNIESVREVSRVLKPNGVLAI 153 555666666689*********87777776665555679***********9987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (219 nodes) Target sequences: 12739 (6856946 residues searched) Passed MSV filter: 421 (0.0330481); expected 254.8 (0.02) Passed bias filter: 205 (0.0160923); expected 254.8 (0.02) Passed Vit filter: 34 (0.00266897); expected 12.7 (0.001) Passed Fwd filter: 11 (0.00086349); expected 0.1 (1e-05) Initial search space (Z): 12739 [actual number of targets] Domain search space (domZ): 11 [number of targets reported over threshold] # CPU time: 0.14u 0.01s 00:00:00.15 Elapsed: 00:00:00.06 # Mc/sec: 25027.85 // [ok]