# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.1b2 (February 2015); http://hmmer.org/
# Copyright (C) 2015 Howard Hughes Medical Institute.
# Freely distributed under the GNU General Public License (GPLv3).
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: pfam_list/Methyltransf_8.hmm.txt
# target sequence database: proteomes/Dictyostelium_discoideum.fasta
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: Methyltransf_8 [M=219]
Accession: PF05148.15
Description: Hypothetical methyltransferase
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
2.7e-80 268.9 6.2 2.7e-80 268.9 6.2 2.5 2 sp|Q54CP1|RRP8_DICDI Ribosomal RNA-processing protein 8 OS
3.2e-06 26.7 0.1 0.00023 20.6 0.1 2.2 1 tr|Q54GQ2|Q54GQ2_DICDI Uncharacterized protein OS=Dictyostel
6.3e-06 25.7 0.0 1.3e-05 24.7 0.0 1.6 1 tr|Q54ET9|Q54ET9_DICDI Uncharacterized protein OS=Dictyostel
7.2e-06 25.5 0.0 0.00025 20.5 0.0 2.2 1 tr|Q55GC0|Q55GC0_DICDI Uncharacterized protein OS=Dictyostel
1.4e-05 24.6 0.0 2.2e-05 23.9 0.0 1.3 1 tr|Q54EN8|Q54EN8_DICDI Uncharacterized protein OS=Dictyostel
7e-05 22.3 0.0 0.00011 21.6 0.0 1.3 1 tr|Q54EP8|Q54EP8_DICDI Uncharacterized protein OS=Dictyostel
7.4e-05 22.2 0.0 0.0011 18.4 0.0 2.5 2 tr|Q553T0|Q553T0_DICDI Uncharacterized protein OS=Dictyostel
0.00097 18.6 0.0 0.012 15.0 0.0 2.2 2 sp|Q54VN2|COQ5_DICDI 2-methoxy-6-polyprenyl-1,4-benzoquino
0.0017 17.7 0.0 0.31 10.3 0.0 2.1 2 tr|Q54SL7|Q54SL7_DICDI Uncharacterized protein OS=Dictyostel
0.0018 17.7 0.7 0.0048 16.3 0.7 1.8 1 tr|Q54LU3|Q54LU3_DICDI Uncharacterized protein OS=Dictyostel
------ inclusion threshold ------
0.052 12.9 0.0 0.15 11.4 0.0 1.7 2 tr|Q54GQ3|Q54GQ3_DICDI Uncharacterized protein OS=Dictyostel
Domain annotation for each sequence (and alignments):
>> sp|Q54CP1|RRP8_DICDI Ribosomal RNA-processing protein 8 OS=Dictyostelium discoideum OX=44689 GN=rrp8 PE=3 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? -2.0 0.3 1.6 1.9e+03 61 102 .. 68 110 .. 58 124 .. 0.67
2 ! 268.9 6.2 2.3e-83 2.7e-80 1 219 [] 157 390 .] 157 390 .] 0.91
Alignments for each domain:
== domain 1 score: -2.0 bits; conditional E-value: 1.6
HHHHHHHCTS-TTS-EEEES-TT-H.HHHH--S-..EEEEESS CS
Methyltransf_8 61 dviirklkkkkdnlvvadlGcGeak.iaeavrekekvrsfdlv 102
++ ++lkk ++n+++ + + k i ++ +++ekv+s d+
sp|Q54CP1|RRP8_DICDI 68 NIKNKNLKKVENNKSLKVISSNKNKnILNNLKKEEKVNSNDIL 110
5555677777777777777766555255777777777777764 PP
== domain 2 score: 268.9 bits; conditional E-value: 2.3e-83
XXXXXX-HHHHHHHHHHHHHHHHHS-HHHHHHHHHH-HHHHHHHHHHHHHHHCTSSS-HHHHHHHHHCTS-TTS-EEEES-TT-HHHHH CS
Methyltransf_8 1 dleakikkrldgarfrflneklytgesskavdlfkedpeafdlyhkgfrsqvkkWPenPldviirklkkkkdnlvvadlGcGeakiaea 89
dl+++++++l g+rfr+lne lyt++s++a++ f ed ++fd yh gf+sqv+ WP nPld ii +l + k+ + +adlGcGeak+ae+
sp|Q54CP1|RRP8_DICDI 157 DLQNEMSEKLKGSRFRWLNETLYTTHSKEAFKEFSEDRSLFDQYHSGFKSQVESWPINPLDLIIDDLSSIKQRKRIADLGCGEAKLAER 245
699************************************************************************************** PP
--S-..EEEEESS-SSTTEEES-TTS-S--TT-EEEEEEES---SS-HHHHHHHHHHHEEEEEEEEEEEEGGG-S-HHHHHHHHHCTTE CS
Methyltransf_8 90 vrekekvrsfdlvavnervvacdlakvPledesvdvavfclslmgtnladflkeanrvlkvGGllkvaevksrfesvklfaraltklGf 178
++k+ ++sfdlvavnerv+acd++++Pl++es+d+avfclslmgtn+ df+ ea+rvl +GGllk+ae++sr+++++ f++++++ Gf
sp|Q54CP1|RRP8_DICDI 246 LQHKHTIQSFDLVAVNERVTACDISNLPLKNESIDIAVFCLSLMGTNFIDFIIEAERVLVKGGLLKIAEIESRITDINAFTNEIQQHGF 334
***************************************************************************************** PP
EEEEEE--STTEEEEEEEE-S.............SS-TT---..GG--------XX CS
Methyltransf_8 179 evkskdlsnkqfvlfefkkve.............kkgkkrel..glklkpclykkr 219
+ +k+++n++f+lfef k + +k kk++ ++ lkpclykkr
sp|Q54CP1|RRP8_DICDI 335 NLIKKNEQNQYFTLFEFSKLQkkdqqfmrslkqyQKLKKQQAtnEPVLKPCLYKKR 390
*****************999833333333322221112222223567889999987 PP
>> tr|Q54GQ2|Q54GQ2_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0188678 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 20.6 0.1 2e-07 0.00023 51 156 .. 23 150 .. 8 192 .. 0.67
Alignments for each domain:
== domain 1 score: 20.6 bits; conditional E-value: 2e-07
HHCTSSS-HHHHHHHHHC...TS-TTS-EEEES-TT-HHHHH-..-S-..EEEEE............SS....-SSTT..EEES-TT CS
Methyltransf_8 51 qvkkWPenPldviirklk...kkkdnlvvadlGcGeakiaeav..rekekvrsfd............lv....avner..vvacdla 114
vk+ P P++ i+ lk + ++dl cG k ++ + ++++v +++ l+ n++ vv +
tr|Q54GQ2|Q54GQ2_DICDI 23 YVKGRPSYPIEA-IKMLKedlGLTIDSKIMDLACGTGKFSKMIceADFKNVVCVEpspefrvdcsyvLKefidDKNKDiqVVNGLAT 108
567777777765.35555222223355677777777777754411345566654311111111111111111122344114444458 PP
S-S--TT-EEEEEEES---SS-HHHHHHHHHHHEEEEEEEEE CS
Methyltransf_8 115 kvPledesvdvavfclslmgtnladflkeanrvlkvGGllkv 156
+P e+++ d + s+ + d +ke+ rvlk+GG+l++
tr|Q54GQ2|Q54GQ2_DICDI 109 SLPFENSTFDCICAAQSFHWYDNVDAIKEITRVLKPGGVLYL 150
9**************************************996 PP
>> tr|Q54ET9|Q54ET9_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0219665 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 24.7 0.0 1.1e-08 1.3e-05 98 169 .. 103 174 .. 67 216 .. 0.74
Alignments for each domain:
== domain 1 score: 24.7 bits; conditional E-value: 1.1e-08
EEESS-SSTTEEES-TTS-S--TT-EE..EEEEES---SS-HHHHHHHHHHHEEEEEEEEEEEEGGG-S-HHHH CS
Methyltransf_8 98 sfdlvavnervvacdlakvPledesvd..vavfclslmgtnladflkeanrvlkvGGllkvaevksrfesvklf 169
s ++ +n +++ c+ ++P e++s d fcl l +t +++lkea+rvl vGG+ v r e+ ++f
tr|Q54ET9|Q54ET9_DICDI 103 SNEISELNFKIQQCNAEQLPFENGSFDryFSNFCLHL-VTSPETMLKEAYRVLEVGGIAAF-SVWGRPENSNQF 174
6677889999*****************555779**98.577899*************9765.355555544443 PP
>> tr|Q55GC0|Q55GC0_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0189501 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 20.5 0.0 2.2e-07 0.00025 95 156 .. 92 153 .. 8 169 .. 0.89
Alignments for each domain:
== domain 1 score: 20.5 bits; conditional E-value: 2.2e-07
.EEEEESS-SSTTEEES-TTS-S--TT-EEEEEEES---SS-HHHHHHHHHHHEEEEEEEEE CS
Methyltransf_8 95 kvrsfdlvavnervvacdlakvPledesvdvavfclslmgtnladflkeanrvlkvGGllkv 156
kv+ + ++n +v + +Pl desvdv + s + + l e rvlkvGG+l +
tr|Q55GC0|Q55GC0_DICDI 92 KVKNEESPSLNYHVLDGISTSIPLPDESVDVLFISQSYHWFDNVQSLTEFSRVLKVGGVLVM 153
555666667788888877799*****************99999999************9865 PP
>> tr|Q54EN8|Q54EN8_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0183885 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 23.9 0.0 1.9e-08 2.2e-05 105 190 .. 112 198 .. 107 216 .. 0.75
Alignments for each domain:
== domain 1 score: 23.9 bits; conditional E-value: 1.9e-08
STTEEES-TTS-S--TT-EE..EEEEES---SS-HHHHHHHHHHHEEEEEEEEEEEEGGG-S-HHHH..HHHH.HCTTEEEEEEE-- CS
Methyltransf_8 105 nervvacdlakvPledesvd..vavfclslmgtnladflkeanrvlkvGGllkvaevksrfesvklf..aral.tklGfevkskdls 186
n +++ c+ k+P d+s d +cl l+ t +++lkea+rvl vGG+ v r e+ ++f ++++ +++G +++s++ s
tr|Q54EN8|Q54EN8_DICDI 112 NFKIQQCNAEKLPFPDNSFDryFSNYCLHLV-TSPETMLKEAYRVLEVGGIAAF-SVWGRPENSNQFtiVKKIaDEIGIDMSSQERS 196
567899**************555779**985.77899*************9765.57788776665522344425788888877654 PP
STTE CS
Methyltransf_8 187 nkqf 190
f
tr|Q54EN8|Q54EN8_DICDI 197 --SF 198
..34 PP
>> tr|Q54EP8|Q54EP8_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0183875 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 21.6 0.0 9.7e-08 0.00011 105 156 .. 108 160 .. 98 204 .. 0.79
Alignments for each domain:
== domain 1 score: 21.6 bits; conditional E-value: 9.7e-08
STTEEES-TTS-S--TT-EE..EEEEES---SS-HHHHHHHHHHHEEEEEEEEE CS
Methyltransf_8 105 nervvacdlakvPledesvd..vavfclslmgtnladflkeanrvlkvGGllkv 156
n +++ c+ k+P d+s d +cl l+ t +++lkea+rvl vGG+
tr|Q54EP8|Q54EP8_DICDI 108 NFKIQQCNAEKLPFPDNSFDryFSNYCLHLV-TSPETMLKEAYRVLEVGGIAAF 160
567999**************555779**985.77899*************8654 PP
>> tr|Q553T0|Q553T0_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0202574 PE=3 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? -0.2 0.0 0.45 5.2e+02 133 158 .. 136 161 .. 134 180 .. 0.86
2 ! 18.4 0.0 9.1e-07 0.0011 108 154 .. 293 340 .. 200 346 .. 0.78
Alignments for each domain:
== domain 1 score: -0.2 bits; conditional E-value: 0.45
-SS-HHHHHHHHHHHEEEEEEEEEEE CS
Methyltransf_8 133 mgtnladflkeanrvlkvGGllkvae 158
m+ ++ fl+ +++ + +G ++k+a
tr|Q553T0|Q553T0_DICDI 136 MTHTWNAFLESIKKTVETGSIVKIAP 161
78899******************995 PP
== domain 2 score: 18.4 bits; conditional E-value: 9.1e-07
EEES-TTS-S--TT-EEEEEEES---.SS-HHHHHHHHHHHEEEEEEE CS
Methyltransf_8 108 vvacdlakvPledesvdvavfclslm.gtnladflkeanrvlkvGGll 154
d k+P d++ dv+ f s + + f+ke +rvlk+GG +
tr|Q553T0|Q553T0_DICDI 293 FDHGDAMKMPYPDNTFDVVTFFESTChMPDKQAFIKECYRVLKPGGRM 340
4567999*************99987515699***************87 PP
>> sp|Q54VN2|COQ5_DICDI 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial OS=Dictyostelium discoideum OX=
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 15.0 0.0 1e-05 0.012 114 158 .. 188 233 .. 159 244 .. 0.85
2 ? 0.9 0.0 0.21 2.4e+02 157 184 .. 272 299 .. 266 307 .. 0.81
Alignments for each domain:
== domain 1 score: 15.0 bits; conditional E-value: 1e-05
TS-S--TT-EEEEEEES---.SS-HHHHHHHHHHHEEEEEEEEEEE CS
Methyltransf_8 114 akvPledesvdvavfclslm.gtnladflkeanrvlkvGGllkvae 158
++P +d++ + + + + tn+ + l+ea+rvlk+GG + e
sp|Q54VN2|COQ5_DICDI 188 EQLPFKDNTFNCYTVSFGIRnCTNIDQVLREAYRVLKPGGRFLCLE 233
68999999998877777774279*****************987666 PP
== domain 2 score: 0.9 bits; conditional E-value: 0.21
EEEGGG-S-HHHHHHHHHCTTEEEEEEE CS
Methyltransf_8 157 aevksrfesvklfaraltklGfevkskd 184
e +f++ ++f++ ++ Gf+++ +
sp|Q54VN2|COQ5_DICDI 272 VESIRKFPDQETFVQMIQDAGFKQVTYK 299
55566899**************997654 PP
>> tr|Q54SL7|Q54SL7_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0204207 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 5.5 0.0 0.0084 9.7 60 97 .. 32 67 .. 22 80 .. 0.70
2 ! 10.3 0.0 0.00027 0.31 126 159 .. 121 154 .. 113 212 .. 0.76
Alignments for each domain:
== domain 1 score: 5.5 bits; conditional E-value: 0.0084
HHHHHHHHCTS-TTS-EEEES-TT-HHHHH--S-..EE CS
Methyltransf_8 60 ldviirklkkkkdnlvvadlGcGeakiaeavrekekvr 97
l+v+++k+ kkkd+ + +GcG++k+ e +++ e v
tr|Q54SL7|Q54SL7_DICDI 32 LKVFLNKFFKKKDK--ILMIGCGNSKLGEDMNDDEFVD 67
56667777777776..5669*******98776654443 PP
== domain 2 score: 10.3 bits; conditional E-value: 0.00027
EEEES---SS-HHHHHHHHHHHEEEEEEEEEEEE CS
Methyltransf_8 126 avfclslmgtnladflkeanrvlkvGGllkvaev 159
av+c +n ++ l e+ rvlk+GG++ v
tr|Q54SL7|Q54SL7_DICDI 121 AVMCSDDDNENAKQILLEVSRVLKPGGFFIVMTY 154
7888888999******************998654 PP
>> tr|Q54LU3|Q54LU3_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0186961 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 16.3 0.7 4.2e-06 0.0048 104 171 .. 126 198 .. 57 241 .. 0.79
Alignments for each domain:
== domain 1 score: 16.3 bits; conditional E-value: 4.2e-06
SSTTEEES-TTS-S--TT-EEE.EEEES---SS..-HHHHHHHHHHHEEEEEEEEEEEE..GGG-S-HHHHHH CS
Methyltransf_8 104 vnervvacdlakvPledesvdv.avfclslmgt..nladflkeanrvlkvGGllkvaev..ksrfesvklfar 171
+n ++ d +++P++d+ d ++ c l+ n + e+ rv+k GG + +++ +s +s+ + ++
tr|Q54LU3|Q54LU3_DICDI 126 PNVKIFIGDASRIPIDDNYFDAyVIRCERLLQHviNPDLVISEMTRVIKSGGRIVITDAdwSSTSVSIPKNIE 198
57788889*************73567999987611556789************99976511444444444444 PP
>> tr|Q54GQ3|Q54GQ3_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0188677 PE=4 SV=1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? -2.7 0.0 2.7 3.1e+03 64 87 .. 39 61 .. 23 78 .. 0.65
2 ? 11.4 0.0 0.00013 0.15 104 156 .. 101 153 .. 79 165 .. 0.79
Alignments for each domain:
== domain 1 score: -2.7 bits; conditional E-value: 2.7
HHHHCTS-TTS-EEEES-TT-HHH CS
Methyltransf_8 64 irklkkkkdnlvvadlGcGeakia 87
i++l ++ v++dl G k +
tr|Q54GQ3|Q54GQ3_DICDI 39 IKSLG-LSNDSVIVDLASGPGKFT 61
34443.356789999999988766 PP
== domain 2 score: 11.4 bits; conditional E-value: 0.00013
SSTTEEES-TTS-S--TT-EEEEEEES---SS-HHHHHHHHHHHEEEEEEEEE CS
Methyltransf_8 104 vnervvacdlakvPledesvdvavfclslmgtnladflkeanrvlkvGGllkv 156
+n +v + +Pl d+svd + + + + ++e+ rvlk+ G+l +
tr|Q54GQ3|Q54GQ3_DICDI 101 LNFKVLEGLSTSIPLPDNSVDCITVATAWHWFSNIESVREVSRVLKPNGVLAI 153
555666666689*********87777776665555679***********9987 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (219 nodes)
Target sequences: 12739 (6856946 residues searched)
Passed MSV filter: 421 (0.0330481); expected 254.8 (0.02)
Passed bias filter: 205 (0.0160923); expected 254.8 (0.02)
Passed Vit filter: 34 (0.00266897); expected 12.7 (0.001)
Passed Fwd filter: 11 (0.00086349); expected 0.1 (1e-05)
Initial search space (Z): 12739 [actual number of targets]
Domain search space (domZ): 11 [number of targets reported over threshold]
# CPU time: 0.14u 0.01s 00:00:00.15 Elapsed: 00:00:00.06
# Mc/sec: 25027.85
//
[ok]