# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Methyltransf_8.hmm.txt # target sequence database: proteomes/Saccharomyces_cerevisiae.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Methyltransf_8 [M=219] Accession: PF05148.15 Description: Hypothetical methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.1e-68 229.9 2.4 3.2e-66 221.8 0.1 2.9 2 sp|P38961|RRP8_YEAST 25S rRNA (adenine(645)-N(1))-methyltran 0.0019 16.5 0.0 0.088 11.1 0.0 2.4 2 sp|P49017|COQ5_YEAST 2-methoxy-6-polyprenyl-1,4-benzoquinol Domain annotation for each sequence (and alignments): >> sp|P38961|RRP8_YEAST 25S rRNA (adenine(645)-N(1))-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 221.8 0.1 1.1e-69 3.2e-66 2 200 .. 113 344 .. 112 357 .. 0.91 2 ! 6.3 0.6 0.00088 2.6 185 219 .] 355 392 .] 345 392 .] 0.74 Alignments for each domain: == domain 1 score: 221.8 bits; conditional E-value: 1.1e-69 XXXXX-HHHHHHHHHHHHHHHHHS-HHHHHHHHHH-HHHHHHHHHHHHHHHCTSSS-HHHHHHHHHC................TS-TTS CS Methyltransf_8 2 leakikkrldgarfrflneklytgesskavdlfkedpeafdlyhkgfrsqvkkWPenPldviirklk................kkkdnl 74 l++k+ +l g+rfr++ne+lyt +s++a +l+ke+p++fd yh+gfrsqv+ WPenP+dv++ +++ k +++ sp|P38961|RRP8_YEAST 113 LQQKMMAKLTGSRFRWINEQLYTISSDEALKLIKEQPQLFDEYHDGFRSQVQAWPENPVDVFVDQIRyrcmkpvnapgglpglKDSKEI 201 79********************************************************************************99999** PP -EEEES-TT-HHH...............HH--S-..EEEEESS-SSTTEEES-TTS-S--TT-EEEEEEES---SS-HHHHHHHHHHHE CS Methyltransf_8 75 vvadlGcGeakia...............eavrekekvrsfdlvavnervvacdlakvPledesvdvavfclslmgtnladflkeanrvl 148 v+ad+GcGea++a + ++ +kv+sfdl+ ner+++ d+++vPl des ++vfcl+lmgtn+ df+kea+r+l sp|P38961|RRP8_YEAST 202 VIADMGCGEAQLAleinnffknynkkakKYLKRRHKVHSFDLKKANERITVADIRNVPLPDESCTIVVFCLALMGTNFLDFIKEAYRIL 290 *************87777665554332222356799***************************************************** PP EEEEEEEEEEEGGG-S..-HHHHHHHHHCTTEEEEEEE--STTEEEEEEEE-SS CS Methyltransf_8 149 kvGGllkvaevksrfe..svklfaraltklGfevkskdlsnkqfvlfefkkvek 200 + G l++ae+ksrf+ +++ f+ al+ +Gf k++ +nk+f+ fef k + sp|P38961|RRP8_YEAST 291 APRGELWIAEIKSRFSdgKGNEFVDALKLMGFFHKKTFDENKMFTRFEFFKPPA 344 ****************444679***************************99763 PP == domain 2 score: 6.3 bits; conditional E-value: 0.00088 --STTEEEEEEEE-SSS-TT---...GG--------XX CS Methyltransf_8 185 lsnkqfvlfefkkvekkgkkrel...glklkpclykkr 219 + ++f+ +e +k e ++k+r++ + lkpc+yk+r sp|P38961|RRP8_YEAST 355 ERRQKFIEVETEKEELEKKRRKIaegKWLLKPCIYKRR 392 45678999999999866666655434788********9 PP >> sp|P49017|COQ5_YEAST 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial OS=Saccharomyces cerevisiae (st # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 11.1 0.0 2.9e-05 0.088 122 160 .. 190 229 .. 182 257 .. 0.75 2 ! 2.8 0.0 0.0099 30 155 181 .. 263 289 .. 258 297 .. 0.84 Alignments for each domain: == domain 1 score: 11.1 bits; conditional E-value: 2.9e-05 -EEEEEEES---.SS-HHHHHHHHHHHEEEEEEEEEEEEG CS Methyltransf_8 122 svdvavfclslm.gtnladflkeanrvlkvGGllkvaevk 160 s d+ + + + t++++ l+ a+rvlk+GG+++ e + sp|P49017|COQ5_YEAST 190 SKDIYTVSFGIRnFTDIQKGLNTAYRVLKPGGIFYCLEFS 229 55555555544314899999**************998864 PP == domain 2 score: 2.8 bits; conditional E-value: 0.0099 EEEEEGGG-S-HHHHHHHHHCTTEEEE CS Methyltransf_8 155 kvaevksrfesvklfaraltklGfevk 181 ++ e rf++ ++f+ ++k Gf+ sp|P49017|COQ5_YEAST 263 YLVESIERFPDQETFKSMIEKAGFKSA 289 5667778*****************976 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (219 nodes) Target sequences: 6049 (2936363 residues searched) Passed MSV filter: 169 (0.0279385); expected 121.0 (0.02) Passed bias filter: 116 (0.0191767); expected 121.0 (0.02) Passed Vit filter: 9 (0.00148785); expected 6.0 (0.001) Passed Fwd filter: 2 (0.000330633); expected 0.1 (1e-05) Initial search space (Z): 6049 [actual number of targets] Domain search space (domZ): 2 [number of targets reported over threshold] # CPU time: 0.07u 0.00s 00:00:00.07 Elapsed: 00:00:00.03 # Mc/sec: 21435.45 // [ok]