# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/Methyltransf_8.hmm.txt # target sequence database: proteomes/Thermococcus_kodakarensis.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Methyltransf_8 [M=219] Accession: PF05148.15 Description: Hypothetical methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.2e-07 27.5 0.0 1.7e-06 25.2 0.0 1.9 1 tr|Q5JFQ4|Q5JFQ4_THEKO SAM-dependent methyltransferase, UbiE 2.1e-06 24.8 0.0 1.1e-05 22.4 0.0 1.9 2 tr|Q5JG60|Q5JG60_THEKO SAM-dependent methyltransferase, UbiE 5.5e-05 20.2 0.0 0.0004 17.4 0.0 2.1 2 tr|Q5JHQ2|Q5JHQ2_THEKO SAM-dependent methyltransferase, UbiE Domain annotation for each sequence (and alignments): >> tr|Q5JFQ4|Q5JFQ4_THEKO SAM-dependent methyltransferase, UbiE/COQ5 family OS=Thermococcus kodakarensis (strain ATCC B # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 25.2 0.0 2.2e-09 1.7e-06 41 160 .. 7 138 .. 1 151 [. 0.70 Alignments for each domain: == domain 1 score: 25.2 bits; conditional E-value: 2.2e-09 HHHHHHHHHHHHC..TSSS-HHHHHHHHHCTS-TTS-EEEES-TT-HHH..HH--S-..EEEEE.S.S-SSTT..EEES-..TTS-S CS Methyltransf_8 41 fdlyhkgfrsqvk..kWPenPldviirklkkkkdnlvvadlGcGeakia..eavrekekvrsfd.l.vavner..vvacd..lakvP 117 fd + + +++ v+ +W + ++++r + ++ ++ vv+d+GcG i + ++ v+ + + +a e+ ++ d + ++P tr|Q5JFQ4|Q5JFQ4_THEKO 7 FDSWAESYDEDVMsdGWIHSDYERVLRLVAER-AKGVVVDVGCGTGNILrfLRCERYIGVEPSEgMrKAFREKwgFEPIDghFLEIP 92 67777888888864469999999999887766.678999*********943333444444433312123333322455552269*** PP --TT-EEEEEEES---...SS-HHHHHHHHHHHEEEEEEEEEEEEG CS Methyltransf_8 118 ledesvdvavfclslm...gtnladflkeanrvlkvGGllkvaevk 160 l ++s+d +v ++ ++ + ++e+ rvl++GG+l +a+v tr|Q5JFQ4|Q5JFQ4_THEKO 93 LPEGSADTVVSTYAFHhvpDEEKGEAIREMLRVLRPGGILIIADVM 138 *******9998877642124677899******************96 PP >> tr|Q5JG60|Q5JG60_THEKO SAM-dependent methyltransferase, UbiE/COQ5 family OS=Thermococcus kodakarensis (strain ATCC B # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 22.4 0.0 1.5e-08 1.1e-05 105 169 .. 76 141 .. 11 173 .. 0.68 2 ? 1.3 0.1 0.043 33 161 186 .. 154 178 .. 147 197 .. 0.72 Alignments for each domain: == domain 1 score: 22.4 bits; conditional E-value: 1.5e-08 STTEEES-TTS-S--TT-EEEEEEES--.-SS-HHHHHHHHHHHEEEEEEEEEEEEGGG-S-HHHH CS Methyltransf_8 105 nervvacdlakvPledesvdvavfclsl.mgtnladflkeanrvlkvGGllkvaevksrfesvklf 169 + +v+ ++P e++s+d ++ ++ +++ ++ lkea+rvlk+GG l + v + ++ + tr|Q5JG60|Q5JG60_THEKO 76 GIEVIEGTAENLPFEEDSMDYLLMVTTIcFVDDPEKALKEAYRVLKPGGALIIGFVDRNSPIGRFY 141 4467777789**********88777665268999*****************999886444333333 PP == domain 2 score: 1.3 bits; conditional E-value: 0.043 GG-S-HHHHHHHHHCTTEEEEEEE-- CS Methyltransf_8 161 srfesvklfaraltklGfevkskdls 186 +rf s + +++ l+k+Gf ++ ++ tr|Q5JG60|Q5JG60_THEKO 154 ARFFSTEELLELLKKVGFREFEI-VQ 178 799***************76443.33 PP >> tr|Q5JHQ2|Q5JHQ2_THEKO SAM-dependent methyltransferase, UbiE/COQ5 family OS=Thermococcus kodakarensis (strain ATCC B # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -0.1 0.0 0.12 88 169 197 .. 52 78 .. 33 89 .. 0.70 2 ! 17.4 0.0 5.2e-07 0.0004 109 155 .. 90 139 .. 81 148 .. 0.85 Alignments for each domain: == domain 1 score: -0.1 bits; conditional E-value: 0.12 HHHHHHCTTEEEEEEE--STTEEEEEEEE CS Methyltransf_8 169 faraltklGfevkskdlsnkqfvlfefkk 197 f l+ lGfev+ l n+ f+l + + tr|Q5JHQ2|Q5JHQ2_THEKO 52 FSFLLEDLGFEVVG--LDNSRFMLEKARE 78 55567889999865..5677888765544 PP == domain 2 score: 17.4 bits; conditional E-value: 5.2e-07 EES-TTS-S--TT-EEEEEEES---S...S-HHHHHHHHHHHEEEEEEEE CS Methyltransf_8 109 vacdlakvPledesvdvavfclslmg...tnladflkeanrvlkvGGllk 155 + d +++P e++s d ++f sl+ ++la+ ke rvlk+GG + tr|Q5JHQ2|Q5JHQ2_THEKO 90 IEGDARELPFENDSFDYVLFIDSLVHfepQDLAKVFKETARVLKPGGKFI 139 567999*****************9963337899999***********875 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (219 nodes) Target sequences: 2301 (637680 residues searched) Passed MSV filter: 93 (0.0404172); expected 46.0 (0.02) Passed bias filter: 77 (0.0334637); expected 46.0 (0.02) Passed Vit filter: 6 (0.00260756); expected 2.3 (0.001) Passed Fwd filter: 3 (0.00130378); expected 0.0 (1e-05) Initial search space (Z): 2301 [actual number of targets] Domain search space (domZ): 3 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 13965.19 // [ok]