# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/SpoU_methylase.hmm.txt # target sequence database: proteomes/Nematostella_vectensis.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: SpoU_methylase [M=142] Accession: PF00588.18 Description: SpoU rRNA Methylase family Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.9e-30 104.3 0.0 9.1e-30 104.1 0.0 1.0 1 tr|A7RT83|A7RT83_NEMVE Predicted protein OS=Nematostella vec 1.5e-28 100.2 0.0 3.5e-28 99.0 0.0 1.6 1 tr|A7SNV9|A7SNV9_NEMVE Predicted protein OS=Nematostella vec 4.4e-21 76.0 0.0 1.6e-20 74.2 0.0 1.8 1 tr|A7S341|A7S341_NEMVE Predicted protein (Fragment) OS=Nemat ------ inclusion threshold ------ 0.049 14.2 0.0 0.3 11.7 0.0 1.9 2 tr|A7SKF0|A7SKF0_NEMVE Predicted protein (Fragment) OS=Nemat Domain annotation for each sequence (and alignments): >> tr|A7RT83|A7RT83_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g92540 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 104.1 0.0 1.5e-33 9.1e-30 2 141 .. 12 153 .. 11 154 .. 0.95 Alignments for each domain: == domain 1 score: 104.1 bits; conditional E-value: 1.5e-33 EEEEEES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHHHHHHCTTHHHHHHEEEE..SSHHHHHHHHCCCTEEEEEEST..T CS SpoU_methylase 2 livvlveiedpgNlGaiaRtakafgaselvlvkprsadllnpeakraaagalevlelkvv..dsleealkelkeegfalvatsl..k 84 l+vv i+++ NlG ++Rt+++fga++lvl + +++ +++p +k +++++ +++++++v ++l+++l+++++ g+ l+++++ + tr|A7RT83|A7RT83_NEMVE 12 LVVVASLINKAPNLGGLCRTCEIFGAKRLVLGS-NEV-MNDPYFKGLSVSSSKWVDIEQVrpAELNNYLDAMRHDGYSLIGVEQtaN 96 799****************************66.666.8*****************99987789******************99889 PP -SEGCCSHHHTTEEEEEEBTTTBS-HHHHCTSSEEEE----TTSSS--HHHHHHHHH CS SpoU_methylase 85 gkslseidlkekvalvfGnEdkGlsnelleladrlvkIpmsgeveSLNvavAvaill 141 +k+l++++++ k++l++GnE++G++ el++++d +v+Ip+ g ++SLNv+v+ a+l+ tr|A7RT83|A7RT83_NEMVE 97 SKRLTDFTFPHKTLLLLGNEKEGIPVELIQQLDACVEIPQVGVIRSLNVHVSGALLI 153 99****************************************************987 PP >> tr|A7SNV9|A7SNV9_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g215116 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 99.0 0.0 5.7e-32 3.5e-28 2 141 .. 129 283 .. 128 284 .. 0.85 Alignments for each domain: == domain 1 score: 99.0 bits; conditional E-value: 5.7e-32 EEEEEES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHHHHHHCTTHHHHHHEEEESSHHHHHHHHCCCTEEEEEEST...T- CS SpoU_methylase 2 livvlveiedpgNlGaiaRtakafgaselvlvkprsadllnpeakraaagalevlelkvvdsleealkelkeegfalvatsl...kg 85 l+++l+ei+dp+N+Gai+Rt +g++++v+ k++sa +l+p + +a+aga ev+++++ ++l ++l+e g++++++ + + tr|A7SNV9|A7SNV9_NEMVE 129 LVLALDEIQDPMNFGAIIRTSWLLGVDKIVVPKQNSA-PLSPVVSKASAGAMEVFNVYQTPDLPKFLQERAAIGWDVLGAVQpitD- 213 789****************************998887.****************************************99877742. PP SEGC......CSHHHTTEEEEEEBTTTBS-HHHHCTSSEEEE--........--TTSSS--HHHHHHHHH CS SpoU_methylase 86 ksls......eidlkekvalvfGnEdkGlsnelleladrlvkIp........msgeveSLNvavAvaill 141 +s + e++l+++++lv+GnE++Gl+n + +++++l++I +s + +S+Nv+vA++il+ tr|A7SNV9|A7SNV9_NEMVE 214 DSHHrpvncaEYSLSKPTVLVLGNEGRGLRNTVQQQCTQLLSIYphqaqnsfQSIDLDSFNVSVAAGILV 283 332234446677888****************************62222222233456899********86 PP >> tr|A7S341|A7S341_NEMVE Predicted protein (Fragment) OS=Nematostella vectensis OX=45351 GN=v1g25116 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 74.2 0.0 2.5e-24 1.6e-20 2 142 .] 91 268 .. 90 268 .. 0.82 Alignments for each domain: == domain 1 score: 74.2 bits; conditional E-value: 2.5e-24 EEEEEES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHHHHHHCTTHHHHHHEEEESSHHHHHHHHCCCTEEEEEEST..T-S CS SpoU_methylase 2 livvlveiedpgNlGaiaRtakafgaselvlvkprsadllnpeakraaagalevlelkvvdsleealkelkeegfalvatsl..kgk 86 l+v++++++dp NlG+i+Rt++a+g+++l+ ++ s dl++p+++r +ag ++ ++l+ s++++ + l + ++l+ +++ gk tr|A7S341|A7S341_NEMVE 91 LTVICDRVKDPSNLGTIVRTCAAAGCQALITTE-YSTDLWDPKVLRGGAGGHFRVPLYGLLSWKQIADMLGDD-TRLYLAESdnTGK 175 78************************9999988.8889****************************9999999.8888887755555 PP EGC...CS.......HHH.......................TTEEEEEEBTTTBS-HHHHCTSS....EEEE----TTSSS--HHHH CS SpoU_methylase 87 sls...ei.......dlk.......................ekvalvfGnEdkGlsnellelad....rlvkIpmsgeveSLNvavA 136 + ++ +++ ++ al++G E++G+s+e+ la + v+Ipm g+++SL + tr|A7S341|A7S341_NEMVE 176 IIIspwKYfvvenpiSWHcygtlnldlgsiiipyfevdwtlGPSALIVGGETTGISTEAQMLACgergQAVHIPMVGNIDSLSAGIS 262 4434442244444452226666666677777777788888****************99876653345579***************** PP HHHHHH CS SpoU_methylase 137 vailly 142 va+++y tr|A7S341|A7S341_NEMVE 263 VALIVY 268 **9988 PP >> tr|A7SKF0|A7SKF0_NEMVE Predicted protein (Fragment) OS=Nematostella vectensis OX=45351 GN=v1g121512 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.7 0.0 4.9e-05 0.3 16 71 .. 29 86 .. 28 96 .. 0.79 2 ? -0.2 0.0 0.22 1.4e+03 64 102 .. 202 244 .. 176 254 .. 0.71 Alignments for each domain: == domain 1 score: 11.7 bits; conditional E-value: 4.9e-05 HHHHHHHHHTT-SEEEEESGSSCTCGSHH..HHHHCTTHHHHHHEEEESSHHHHHHHH CS SpoU_methylase 16 GaiaRtakafgaselvlvkprsadllnpe..akraaagalevlelkvvdsleealkel 71 G iaR++ +f+++e+++ ++ +++l++++ + ++ ++a l+l+++ ++ e+ + l tr|A7SKF0|A7SKF0_NEMVE 29 GQIARAVVVFNVDEVIVFNESRKSLDKSKdgVSNFHSKADANLQLARILQYLECPQYL 86 99*****************988777666666888888888888877666666655555 PP == domain 2 score: -0.2 bits; conditional E-value: 0.22 HHHHHHHHCCC.TEEE.EEESTT-SEGCCSHHH..TTEEEEEE CS SpoU_methylase 64 leealkelkee.gfal.vatslkgkslseidlk..ekvalvfG 102 ++++++e g+++ ++ts++g +++ ++l+ +++++vfG tr|A7SKF0|A7SKF0_NEMVE 202 FSDVFTESPYPqGYDVmIGTSERGAPVDCLELQpfSHMLIVFG 244 5555444443326777688888999999666666699999999 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (142 nodes) Target sequences: 24428 (8257945 residues searched) Passed MSV filter: 445 (0.0182168); expected 488.6 (0.02) Passed bias filter: 399 (0.0163337); expected 488.6 (0.02) Passed Vit filter: 37 (0.00151466); expected 24.4 (0.001) Passed Fwd filter: 4 (0.000163747); expected 0.2 (1e-05) Initial search space (Z): 24428 [actual number of targets] Domain search space (domZ): 4 [number of targets reported over threshold] # CPU time: 0.11u 0.01s 00:00:00.12 Elapsed: 00:00:00.06 # Mc/sec: 19543.80 // [ok]