# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/SpoU_methylase.hmm.txt # target sequence database: proteomes/Saccharomyces_cerevisiae.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: SpoU_methylase [M=142] Accession: PF00588.18 Description: SpoU rRNA Methylase family Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.2e-26 91.2 0.0 4.6e-26 90.2 0.0 1.6 1 sp|Q07527|TRM3_YEAST tRNA (guanosine(18)-2'-O)-methyltransfe 6.1e-26 89.8 0.0 2.6e-25 87.7 0.0 2.0 1 sp|P25270|MRM1_YEAST rRNA methyltransferase 1, mitochondrial Domain annotation for each sequence (and alignments): >> sp|Q07527|TRM3_YEAST tRNA (guanosine(18)-2'-O)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 90.2 0.0 1.5e-29 4.6e-26 2 141 .. 1286 1428 .. 1285 1429 .. 0.94 Alignments for each domain: == domain 1 score: 90.2 bits; conditional E-value: 1.5e-29 EEEEEES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHHHHHHCTTHHHHHHEEEE..SSHHHHHHHHCCCTEEEEEESTT-S CS SpoU_methylase 2 livvlveiedpgNlGaiaRtakafgaselvlvkprsadllnpeakraaagalevlelkvv..dsleealkelkeegfalvatslkgk 86 livv +++p NlG i+R+++++g+ +l+ v++ ++ ++p++k++a+ a ++++++v d++++++ke k+eg++l+++++ +k sp|Q07527|TRM3_YEAST 1286 LIVVSSLVDKPPNLGGICRLCDVLGV-GLLTVQDIKV-KNHPQFKNVAVTADRWMPMEEValDEIASFMKEKKKEGYTLIGLEQTDK 1370 7899999*******************.8888887887.9*****************9988779*********************999 PP EGC...CSHHHTTEEEEEEBTTTBS-HHHHCTSSEEEE----TTSSS--HHHHHHHHH CS SpoU_methylase 87 sls...eidlkekvalvfGnEdkGlsnelleladrlvkIpmsgeveSLNvavAvaill 141 s++ ++++++k ++++G+E G++ ll+++d +++I + g ++S+N+++A+a+++ sp|Q07527|TRM3_YEAST 1371 SVKldnNFQFPKKSLILLGTEAFGIPGTLLSELDLCLEIQQFGVIRSMNIQTATAVIV 1428 88887899************************************************97 PP >> sp|P25270|MRM1_YEAST rRNA methyltransferase 1, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 87.7 0.0 8.6e-29 2.6e-25 4 141 .. 244 405 .. 241 406 .. 0.80 Alignments for each domain: == domain 1 score: 87.7 bits; conditional E-value: 8.6e-29 EEEES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHHHHHHCTTHHHHHHEEEESSHHHHHHHHCCCTEEEEEEST..T-SEGC. CS SpoU_methylase 4 vvlveiedpgNlGaiaRtakafgaselvlvkprsadllnpeakraaagalevlelkvvdsleealkelkeegfalvatsl..kgksls. 89 ++l+ei+dp+N+Gai+R+a+ +g++ +v++++++ ++l+p + ++++gale+l++ +vd+ e++++ +e g +++ ts+ + +s sp|P25270|MRM1_YEAST 244 LYLDEITDPHNIGAIIRSAYFLGVDFIVMSRRNC-SPLTPVVSKTSSGALELLPIFYVDKPLEFFTKSQEMGGWTFITSHlaN--ATSe 329 79**********************8888877666.6***********************************999999988543..3332 PP CS.......HHH........TTEEEEEEBTTTBS-HHHHCTSSEEEE----TT..........SSS--HHHHHHHHH CS SpoU_methylase 90 ei.......dlk........ekvalvfGnEdkGlsnelleladrlvkIpmsge..........veSLNvavAvaill 141 ++ + +v+lv+GnE++G++++l + d +v+Ip g v+SLNv+vA+a+l+ sp|P25270|MRM1_YEAST 330 KYtvgktisM-HdlnglcneLPVVLVVGNESQGVRTNLKMRSDFFVEIPFGGIekgnrapepiVDSLNVSVATALLI 405 2233443440.2555777779****************************7532222233333389*********986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (142 nodes) Target sequences: 6049 (2936363 residues searched) Passed MSV filter: 119 (0.0196727); expected 121.0 (0.02) Passed bias filter: 101 (0.016697); expected 121.0 (0.02) Passed Vit filter: 13 (0.00214912); expected 6.0 (0.001) Passed Fwd filter: 2 (0.000330633); expected 0.1 (1e-05) Initial search space (Z): 6049 [actual number of targets] Domain search space (domZ): 2 [number of targets reported over threshold] # CPU time: 0.05u 0.00s 00:00:00.05 Elapsed: 00:00:00.02 # Mc/sec: 20848.18 // [ok]