# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/SpoU_methylase.hmm.txt # target sequence database: proteomes/Strongylocentrotus_purpuratus.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: SpoU_methylase [M=142] Accession: PF00588.18 Description: SpoU rRNA Methylase family Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.3e-27 97.3 0.0 3.7e-27 95.9 0.0 1.8 2 tr|W4Y7I5|W4Y7I5_STRPU Uncharacterized protein OS=Strongyloc 5.1e-27 95.4 0.0 1.3e-26 94.1 0.0 1.7 1 tr|W4Y3Y0|W4Y3Y0_STRPU Uncharacterized protein OS=Strongyloc 2.5e-25 90.0 0.0 5.3e-12 46.8 0.0 2.2 2 tr|W4YB11|W4YB11_STRPU Uncharacterized protein OS=Strongyloc Domain annotation for each sequence (and alignments): >> tr|W4Y7I5|W4Y7I5_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -3.5 0.0 1.7 1.6e+04 62 83 .. 348 369 .. 334 378 .. 0.79 2 ! 95.9 0.0 3.9e-31 3.7e-27 2 137 .. 410 555 .. 409 556 .. 0.88 Alignments for each domain: == domain 1 score: -3.5 bits; conditional E-value: 1.7 SSHHHHHHHHCCCTEEEEEEST CS SpoU_methylase 62 dsleealkelkeegfalvatsl 83 ++++++l+ ++e+g+++++t + tr|W4Y7I5|W4Y7I5_STRPU 348 SEVKDILALVEERGLRVWTTNR 369 6678889999999999998866 PP == domain 2 score: 95.9 bits; conditional E-value: 3.9e-31 EEEEEES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHHHHHHCTTHHHHHHEEEESSHHHHHHHHCCCTEEEEEESTT-SEG CS SpoU_methylase 2 livvlveiedpgNlGaiaRtakafgaselvlvkprsadllnpeakraaagalevlelkvvdsleealkelkeegfalvatslkgksl 88 +++vl++i+dp+NlGa++R+a+ +g+++++++k+++ l+p + +a++g e+++++ + + ++l++ k +g+ +v++s +g+s tr|W4Y7I5|W4Y7I5_STRPU 410 VRLVLDQIKDPMNLGALLRSAYFLGVDKVIVSKKNCC-SLTPVVSKASSGVMEIIPVYATALIPSYLENEKLKGWSIVGASVGGSSS 495 799******************************9999.9*******************************************66655 PP C.CS....HHH..TTEEEEEEBTTTBS-HHHHCTSSEEEE---....-TTSSS--HHHHH CS SpoU_methylase 89 s.ei....dlk..ekvalvfGnEdkGlsnelleladrlvkIpm....sgeveSLNvavAv 137 s ++ +++ ++v+lv+GnE+ Gls ++ e+++++++Ip + ++SLNv+vA+ tr|W4Y7I5|W4Y7I5_STRPU 496 SeDVipchEYNptGPVLLVVGNEGFGLSSDISEQCSEFISIPAgrqlHAGIDSLNVSVAT 555 5333333333356*****************************8443455679*******7 PP >> tr|W4Y3Y0|W4Y3Y0_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 94.1 0.0 1.4e-30 1.3e-26 2 141 .. 1489 1630 .. 1488 1631 .. 0.94 Alignments for each domain: == domain 1 score: 94.1 bits; conditional E-value: 1.4e-30 EEEEEES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHHHHHHCTTHHHHHHEEEE..SSHHHHHHHHCCCTEEEEEEST. CS SpoU_methylase 2 livvlveiedpgNlGaiaRtakafgaselvlvkprsadllnpeakraaagalevlelkvv..dsleealkelkeegfalvatsl. 83 l+vv i+ NlG ++Rt+++fgas+lvl + + ++++++ ++++a+ +l+l++v ++l++++ e+k++g++lv++++ tr|W4Y3Y0|W4Y3Y0_STRPU 1489 LVVVASLISRVPNLGGLCRTCEIFGASCLVLGN-LAL-KDHQDFLSLSVSAERWLPLREVkpSDLKDYMIEMKKHGYTLVGVEQt 1571 7899999************************76.443.69999*************99997789******************998 PP .T-SEGCCSHHHTTEEEEEEBTTTBS-HHHHCTSSEEEE----TTSSS--HHHHHHHHH CS SpoU_methylase 84 .kgkslseidlkekvalvfGnEdkGlsnelleladrlvkIpmsgeveSLNvavAvaill 141 +++sl++++++++++l++G+E++G++ el++ +d +v+Ip+ g ++SLNv+v+ a+++ tr|W4Y3Y0|W4Y3Y0_STRPU 1572 aNSQSLTDFKFPQRTMLLLGKEREGIPVELIQFLDVCVEIPQLGFIRSLNVHVSGALMI 1630 8999999*************************************************997 PP >> tr|W4YB11|W4YB11_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 46.8 0.0 5.5e-16 5.3e-12 2 60 .. 99 156 .. 98 176 .. 0.88 2 ! 41.4 0.0 2.5e-14 2.4e-10 45 142 .] 191 293 .. 169 293 .. 0.71 Alignments for each domain: == domain 1 score: 46.8 bits; conditional E-value: 5.5e-16 EEEEEES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHHHHHHCTTHHHHHHEEE CS SpoU_methylase 2 livvlveiedpgNlGaiaRtakafgaselvlvkprsadllnpeakraaagalevlelkv 60 + +++++i+dpgNlG+i+R+a+a+ +++++l+k ++d+++p+++r++aga++ +++ tr|W4YB11|W4YB11_STRPU 99 VSLICDNIRDPGNLGTILRSAAAAACQKILLTK-GCVDVWEPKVLRSGAGAHFRVPIIS 156 6799***************************88.*********************9644 PP == domain 2 score: 41.4 bits; conditional E-value: 2.5e-14 HHHHCTTHHHHHHEEEESSHHHHHHHHCCCTEEE.EEESTT-SEGCCSHHH.TTEEEEEEBTTTBS-HHHHCTSS....EEEE---- CS SpoU_methylase 45 akraaagalevlelkvvdsleealkelkeegfal.vatslkgkslseidlk.ekvalvfGnEdkGlsnellelad....rlvkIpms 125 + a+ ++ e+ + ++d+ +++ ++ ++ ++ at+ ++ ++ ++++ + alv+G E++Gls+e+ el++ r + Ipms tr|W4YB11|W4YB11_STRPU 191 SHGASENQSEYAQ-NEYDNDDDISADHSKVMDETnAATDVRTVPYFSVNWTqGDYALVIGGETEGLSREAKELCTetggRRIFIPMS 276 2333444444444.78888888888888874444144444888888888888999*******************95444589***** PP TTSSS--HHHHHHHHHH CS SpoU_methylase 126 geveSLNvavAvailly 142 ++SLN a+A++++l+ tr|W4YB11|W4YB11_STRPU 277 MGIDSLNAAMATSVILF 293 **************985 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (142 nodes) Target sequences: 28594 (14266044 residues searched) Passed MSV filter: 573 (0.0200392); expected 571.9 (0.02) Passed bias filter: 509 (0.0178009); expected 571.9 (0.02) Passed Vit filter: 37 (0.00129398); expected 28.6 (0.001) Passed Fwd filter: 3 (0.000104917); expected 0.3 (1e-05) Initial search space (Z): 28594 [actual number of targets] Domain search space (domZ): 3 [number of targets reported over threshold] # CPU time: 0.17u 0.01s 00:00:00.18 Elapsed: 00:00:00.09 # Mc/sec: 22508.65 // [ok]