# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/SpoU_methylase.hmm.txt # target sequence database: proteomes/Tetrahymena_thermophila.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: SpoU_methylase [M=142] Accession: PF00588.18 Description: SpoU rRNA Methylase family Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.8e-28 99.5 0.0 1.9e-27 96.8 0.0 2.2 1 tr|I7MJ42|I7MJ42_TETTS TrmH family RNA methyltransferase OS= 1.2e-26 94.2 0.0 3.8e-26 92.5 0.0 2.0 1 tr|I7MFK2|I7MFK2_TETTS TrmH family RNA methyltransferase OS= 2.6e-24 86.6 0.2 7.7e-22 78.6 0.2 2.2 1 tr|I7M2R4|I7M2R4_TETTS TrmH family RNA methyltransferase OS= 0.0051 17.6 0.2 2e+02 2.7 0.0 6.6 10 tr|Q24CU0|Q24CU0_TETTS Tetratricopeptide repeat protein OS=T ------ inclusion threshold ------ 0.21 12.3 0.3 0.55 11.0 0.1 1.8 2 tr|A4VDU8|A4VDU8_TETTS Uncharacterized protein OS=Tetrahymen Domain annotation for each sequence (and alignments): >> tr|I7MJ42|I7MJ42_TETTS TrmH family RNA methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHE # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 96.8 0.0 3.5e-31 1.9e-27 2 141 .. 123 278 .. 122 279 .. 0.83 Alignments for each domain: == domain 1 score: 96.8 bits; conditional E-value: 3.5e-31 EEEEEES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHHHHHHCTTHHHHHHEEEESSHHHHHHHHCCCTEEEEEEST..T-S CS SpoU_methylase 2 livvlveiedpgNlGaiaRtakafgaselvlvkprsadllnpeakraaagalevlelkvvdsleealkelkeegfalvatsl..kgk 86 +i+ l++i+dp+N+Gai+Rt++ +g++++v+ ++ s +lnp++ ++++galev++++ ++ e+lk+++ +g+++++t l +++ tr|I7MJ42|I7MJ42_TETTS 123 MILFLDRITDPQNFGAIIRTCQYLGVDNVVV-DEFSRCPLNPTVSKTSSGALEVMDIYCTPDPVEFLKDCQLRGWKVYSTGLnhSEN 208 6899**********************99988.5566669**************************************9997754333 PP EGC....CSHHHTTEEEEEEBTTTBS-HHHHCTSSEEEE-........--...-TTSSS--HHHHHHHHH CS SpoU_methylase 87 sls....eidlkekvalvfGnEdkGlsnelleladrlvkI........pm...sgeveSLNvavAvaill 141 ++s +++ k+k++lv+GnE++G+++++l+ d++v+I pm + ++SLNv A++il+ tr|I7MJ42|I7MJ42_TETTS 209 QISleklKVSSKDKIVLVMGNEGNGVDPKILKVSDKIVSIdqtlylnrPMvfpYTLIDSLNVNSATSILI 278 444555555777****************************444444443432234468999999999986 PP >> tr|I7MFK2|I7MFK2_TETTS TrmH family RNA methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHE # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 92.5 0.0 7e-30 3.8e-26 2 141 .. 1578 1719 .. 1577 1720 .. 0.92 Alignments for each domain: == domain 1 score: 92.5 bits; conditional E-value: 7e-30 EEEEEES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHHHHHHCTTHHHHHHEEEE..SSHHHHHHHHCCCTEEEEEEST. CS SpoU_methylase 2 livvlveiedpgNlGaiaRtakafgaselvlvkprsadllnpeakraaagalevlelkvv..dsleealkelkeegfalvatsl. 83 +ivv +e+ N+G + Rt ++fg+s lv+ + +a l ++ +k++++ a+++l++++v ++l ++l k+ g+++v++++ tr|I7MFK2|I7MFK2_TETTS 1578 IIVVASLLEKIPNFGHLTRTSEIFGVSSLVIPN--KAILEEEGFKAISVTAEKWLPIQEVkeKDLMAFLILKKKLGYKIVGLEQt 1660 78999999***********************66..55588888*************9999667899999999999*******998 PP .T-SEGCCSHHHTTEEEEEEBTTTBS-HHHHCTSSEEEE----TTSSS--HHHHHHHHH CS SpoU_methylase 84 .kgkslseidlkekvalvfGnEdkGlsnelleladrlvkIpmsgeveSLNvavAvaill 141 k+k ++ ++++k++l++G+E++G++ e +e +d++v+Ip+ g+v+SLNv+v++ i++ tr|I7MFK2|I7MFK2_TETTS 1661 sKSKMIQHQEFPDKCILLLGREKTGIPLEYIEILDECVEIPQFGQVRSLNVHVSAVICI 1719 788888899************************************************98 PP >> tr|I7M2R4|I7M2R4_TETTS TrmH family RNA methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHE # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 78.6 0.2 1.4e-25 7.7e-22 3 141 .. 20 179 .. 18 180 .. 0.81 Alignments for each domain: == domain 1 score: 78.6 bits; conditional E-value: 1.4e-25 EEEEES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHH..................HHHH.CTTHHHHHHEEEESSHHHHHHH CS SpoU_methylase 3 ivvlveiedpgNlGaiaRtakafgaselvlvkprsadllnpe..................akra.aagalevlelkvvdsleealke 70 +++l +i +++N+G i+R+a+af++++++l+++ ++d++n+e ++ + +g++ ++++ v+++l+ea + tr|I7M2R4|I7M2R4_TETTS 20 YLLLHNIGKKNNMGIIIRSASAFNFEKVFLISK-NEDIFNEEvegderkrkrvlhrvvkeFQMFgNHGTQAQMQFDVFKNLKEAKQY 105 5789****************************5.55788888566666666766665333222224567777778888888888888 PP HCCCTEEEEEEST..T-SEGCCSHHHTTEEEEEEBTTTBS-HHHHCTSSEEEE---.-TTSSS--HHHHHHHHH CS SpoU_methylase 71 lkeegfalvatsl..kgkslseidlkekvalvfGnEdkGlsnelleladrlvkIpm.sgeveSLNvavAvaill 141 + ++++++++++ ++ks+ + +++ +++++GnE++Gl + + +d++v+Ip+ +++ SLNv+ A++i++ tr|I7M2R4|I7M2R4_TETTS 106 FVQNNIKVCGVEItpDSKSVVTHPFQGDTVFLMGNEGAGLIEAHKKICDHFVYIPQyTNKTASLNVSCAASIIF 179 88888888777666688888888999******************************66788***********96 PP >> tr|Q24CU0|Q24CU0_TETTS Tetratricopeptide repeat protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHER # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.5 0.0 1.5 7.9e+03 60 81 .. 86 106 .. 58 133 .. 0.53 2 ? -2.0 0.0 1 5.5e+03 112 136 .. 357 381 .. 355 383 .. 0.88 3 ? -3.2 0.0 2.4 1.3e+04 106 136 .. 385 415 .. 384 416 .. 0.83 4 ? -1.1 0.0 0.53 2.8e+03 105 136 .. 418 449 .. 418 451 .. 0.89 5 ? 2.0 0.0 0.059 3.2e+02 106 136 .. 487 517 .. 486 519 .. 0.93 6 ? 1.0 0.0 0.12 6.4e+02 106 136 .. 521 551 .. 520 552 .. 0.89 7 ? 2.7 0.0 0.037 2e+02 104 136 .. 553 585 .. 552 587 .. 0.87 8 ? -2.2 0.0 1.2 6.5e+03 106 136 .. 589 619 .. 588 621 .. 0.87 9 ? -3.4 0.0 2.7 1.5e+04 106 133 .. 623 650 .. 622 653 .. 0.87 10 ? -0.1 0.0 0.26 1.4e+03 105 136 .. 656 687 .. 656 689 .. 0.92 Alignments for each domain: == domain 1 score: -2.5 bits; conditional E-value: 1.5 EESSHHHHHHHHCCCTEEEEEE CS SpoU_methylase 60 vvdsleealkelkeegfalvat 81 ++++ ++++e++ f+ + + tr|Q24CU0|Q24CU0_TETTS 86 NHQNIVKYCDEFQIG-FNYFIV 106 455555555444443.333222 PP == domain 2 score: -2.0 bits; conditional E-value: 1 HHCTSSEEEE----TTSSS--HHHH CS SpoU_methylase 112 lleladrlvkIpmsgeveSLNvavA 136 ++ ++++++I ++++ +N+a A tr|Q24CU0|Q24CU0_TETTS 357 SIKSYQKCLEINPKKDICFMNLAIA 381 6788999****************98 PP == domain 3 score: -3.2 bits; conditional E-value: 2.4 TBS-HHHHCTSSEEEE----TTSSS--HHHH CS SpoU_methylase 106 kGlsnelleladrlvkIpmsgeveSLNvavA 136 kG+ +e+++ ++++++I +++ N+ A tr|Q24CU0|Q24CU0_TETTS 385 KGMIDEAIKSYKKCLEINPKEDSCYYNLGIA 415 79999999********999888877777665 PP == domain 4 score: -1.1 bits; conditional E-value: 0.53 TTBS-HHHHCTSSEEEE----TTSSS--HHHH CS SpoU_methylase 105 dkGlsnelleladrlvkIpmsgeveSLNvavA 136 dkG+ +e++++++++++I ++e+ N+ A tr|Q24CU0|Q24CU0_TETTS 418 DKGMLDEAIKAYQKCLEINPKKEICFYNLGIA 449 589999*************9999999998877 PP == domain 5 score: 2.0 bits; conditional E-value: 0.059 TBS-HHHHCTSSEEEE----TTSSS--HHHH CS SpoU_methylase 106 kGlsnelleladrlvkIpmsgeveSLNvavA 136 kGl +e+++ ++++++I ++++ +N+ A tr|Q24CU0|Q24CU0_TETTS 487 KGLLDEAIKSYQKCIEINPKKDIYYMNLGLA 517 799************************9877 PP == domain 6 score: 1.0 bits; conditional E-value: 0.12 TBS-HHHHCTSSEEEE----TTSSS--HHHH CS SpoU_methylase 106 kGlsnelleladrlvkIpmsgeveSLNvavA 136 kG+ ne++++++++++I +++ +N+ A tr|Q24CU0|Q24CU0_TETTS 521 KGMLNEAIKQYQKCIEINPKEDSCYFNLGIA 551 8999**************9999999998776 PP == domain 7 score: 2.7 bits; conditional E-value: 0.037 TTTBS-HHHHCTSSEEEE----TTSSS--HHHH CS SpoU_methylase 104 EdkGlsnelleladrlvkIpmsgeveSLNvavA 136 E+kG+s e+++ ++++v+I + + N+ A tr|Q24CU0|Q24CU0_TETTS 553 ENKGMSGEAIKSYQKCVEINPQHDSCLYNLGIA 585 99*****************98888777777766 PP == domain 8 score: -2.2 bits; conditional E-value: 1.2 TBS-HHHHCTSSEEEE----TTSSS--HHHH CS SpoU_methylase 106 kGlsnelleladrlvkIpmsgeveSLNvavA 136 kG+ +e+++ ++++++I ++++ N+ A tr|Q24CU0|Q24CU0_TETTS 589 KGMLDEAIKSYQKCLEINPKKDICLYNLGIA 619 799999***********99999998888877 PP == domain 9 score: -3.4 bits; conditional E-value: 2.7 TBS-HHHHCTSSEEEE----TTSSS--H CS SpoU_methylase 106 kGlsnelleladrlvkIpmsgeveSLNv 133 kG+ +e+++ + ++++I + + +N+ tr|Q24CU0|Q24CU0_TETTS 623 KGVYDEAIKSYYKCLEINPKHDNCHMNL 650 7999999**********99999888887 PP == domain 10 score: -0.1 bits; conditional E-value: 0.26 TTBS-HHHHCTSSEEEE----TTSSS--HHHH CS SpoU_methylase 105 dkGlsnelleladrlvkIpmsgeveSLNvavA 136 dkG+ +++++ +++++kI + ++ +N+ A tr|Q24CU0|Q24CU0_TETTS 656 DKGMLDDAIKSYQKCLKINPKHDICYMNLGIA 687 589999**********************9988 PP >> tr|A4VDU8|A4VDU8_TETTS Uncharacterized protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_0012535 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.8 0.0 1.7 9.4e+03 111 136 .. 128 154 .. 99 156 .. 0.65 2 ? 11.0 0.1 0.0001 0.55 6 57 .. 215 266 .. 212 289 .. 0.82 Alignments for each domain: == domain 1 score: -2.8 bits; conditional E-value: 1.7 HHHCTSS.EEEE----TTSSS--HHHH CS SpoU_methylase 111 ellelad.rlvkIpmsgeveSLNvavA 136 + l++++ l+ I +++ +S N ++A tr|A4VDU8|A4VDU8_TETTS 128 NYLQKCKiILILIQKQESESSSNWSQA 154 445666633444555555556666665 PP == domain 2 score: 11.0 bits; conditional E-value: 0.0001 EES-SSHHHHHHHHHHHHHTT-SEEEEESGSSCTCGSHHHHHHCTTHHHHHH CS SpoU_methylase 6 lveiedpgNlGaiaRtakafgaselvlvkprsadllnpeakraaagalevle 57 + i++++N+++i+ + ++ +++l ++ +++++l+++++ + ++++++ + tr|A4VDU8|A4VDU8_TETTS 215 MKIIQHEQNFSKIINFCLNTPVKQLKICTQENFQLDSQKVSLIIQHNQQIQK 266 5569*****************************9999999988888877776 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (142 nodes) Target sequences: 26971 (16912921 residues searched) Passed MSV filter: 919 (0.0340736); expected 539.4 (0.02) Passed bias filter: 521 (0.019317); expected 539.4 (0.02) Passed Vit filter: 38 (0.00140892); expected 27.0 (0.001) Passed Fwd filter: 5 (0.000185384); expected 0.3 (1e-05) Initial search space (Z): 26971 [actual number of targets] Domain search space (domZ): 5 [number of targets reported over threshold] # CPU time: 0.23u 0.01s 00:00:00.24 Elapsed: 00:00:00.11 # Mc/sec: 21833.04 // [ok]