# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/TRM.hmm.txt # target sequence database: proteomes/Amphimedon_queenslandica.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TRM [M=376] Accession: PF02005.15 Description: N2,N2-dimethylguanosine tRNA methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.5e-128 429.2 0.0 4.5e-128 428.9 0.0 1.0 1 tr|A0A1X7UT60|A0A1X7UT60_AMPQE tRNA (guanine(26)-N(2))-dimethyl 2.3e-31 110.6 0.0 2.6e-31 110.4 0.0 1.0 1 tr|A0A1X7SIB7|A0A1X7SIB7_AMPQE Uncharacterized protein OS=Amphi 3.6e-05 24.4 0.0 7.6e-05 23.3 0.0 1.5 1 tr|A0A1X7VCI4|A0A1X7VCI4_AMPQE Uncharacterized protein OS=Amphi ------ inclusion threshold ------ 0.28 11.6 0.0 0.41 11.0 0.0 1.2 1 tr|A0A1X7UNT6|A0A1X7UNT6_AMPQE Uncharacterized protein OS=Amphi 0.37 11.2 0.0 0.6 10.5 0.0 1.3 1 tr|A0A1X7VVN5|A0A1X7VVN5_AMPQE Uncharacterized protein OS=Amphi Domain annotation for each sequence (and alignments): >> tr|A0A1X7UT60|A0A1X7UT60_AMPQE tRNA (guanine(26)-N(2))-dimethyltransferase OS=Amphimedon queenslandica OX=400682 PE= # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 428.9 0.0 5.2e-132 4.5e-128 8 376 .] 53 482 .. 45 482 .. 0.92 Alignments for each domain: == domain 1 score: 428.9 bits; conditional E-value: 5.2e-132 ----SS--TT--SS--GGGHHHHHHHHHH...HHHHHHHH-S-.................................... CS TRM 8 edkevtvskkeeVFYNPvqefnRDlsvlvirqlellkkklgek.................................... 50 ++ + v+k++ VFYNPvq fnRDls++v+ q + + + + tr|A0A1X7UT60|A0A1X7UT60_AMPQE 53 GKARMFVPKQNSVFYNPVQVFNRDLSLAVVDQYAKKYWRY--Hhrqkrpfwradsglianekkpnepaepaksdssnne 129 3444779**********************85322222222..1224578****************************** PP .....EEEEESS-TTSHHHHHHHHH-S-E.EEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HHHHHC...--SS- CS TRM 51 .....ikvldaLsasGlRaiRfalEvpgv.kevlaNDisekAvelikeNvklneveeivvineddAnvlla...ekkkr 120 +k+l+aL+asGlR+iR+alE++++ e+ aND s+ A+++i N+k n v++iv+++ +dA++l++ e ++ tr|A0A1X7UT60|A0A1X7UT60_AMPQE 130 pdrpkLKILEALAASGLRSIRYALEIQSTpCEITANDYSSDAYQSITDNAKRNGVSKIVQPSCKDARLLMYehiEPLEQ 208 **************************976369***************************************7766779* PP EEEEEE--SS--GGGHHHHHHHEEEEEEEEEEE--HHHHTTSSHHHHHHHHSSB---STTHHHHHHHHHHHHHHHHHHT CS TRM 121 fdviDlDPfGspapfldsavqsvkekglLlvTaTDtavLaGaspekclrkYgavalkselcHEvalRillkavareaak 199 f+v+D+DP+Gs+a fldsavq++k++glL+vT+TD +vL+G+ e c++kYg+v+lk+++cHE++lRi+l +++++a + tr|A0A1X7UT60|A0A1X7UT60_AMPQE 209 FHVVDIDPYGSAAVFLDSAVQCIKDGGLLCVTCTDASVLCGNRSEVCFSKYGSVSLKTKYCHEMGLRIILASLNSHAGR 287 ******************************************************************************* PP TTEEEEEEEEEEETTEEEEEEEEEE-HHHHHHHHTTEEEEEEETTT..........--EEEE--GGG--.......SB- CS TRM 200 YekavepllslsadfYvrvfvkvktgaakvkkvleklglvlhCsgC..........lseekvkglakfk.......aec 261 Y+++++p+lsls+dfY+rvfv + t++ +vkk+++kl +v+hC gC ls+ k +++++ + ++c tr|A0A1X7UT60|A0A1X7UT60_AMPQE 288 YQRYIHPILSLSTDFYFRVFVTIYTSPLEVKKSASKLSMVYHCDGCssfhlqplikLSTTKDNSIKTHSsfgppinKDC 366 **********************************************9999999988666666777766688899999** PP TTT-SBEEEEEEEE-S-S--HHHHHHHHHHHCT..-TTS.-HHHHHHHHHHHHHHS--SS-EEHHHHHHHHT-SS---H CS TRM 262 ehcgaklhlaGPlwlgplkdkefveevleeaekekeeel.kkrvkklLklikeElldaplyydlsqlasvlklsvpplk 339 ehc+++++++GP+w +p++d f+ ++l ++++k+ + ++ ++L+++keEl ++p+++dlsql+svl+++v ++ tr|A0A1X7UT60|A0A1X7UT60_AMPQE 367 EHCSSNFKFGGPVWSDPIHDDIFISSLLSDLQETKDCFAtNSKMIGMLSMMKEELNNVPFFHDLSQLSSVLHCNVMRML 445 *********************************8754446999************************************ PP HHHHHH....TT-EE-TTSTTEEE-SS-HHHHHHHHH CS TRM 340 kvvsalkelgfevsrtHakpkaikTnapleavvevlr 376 +++sal + g++vs +H++p+a+kT+ap ++v++++r tr|A0A1X7UT60|A0A1X7UT60_AMPQE 446 EMRSALMNQGYRVSSSHTNPQAVKTDAPHSVVWDIMR 482 ************************************7 PP >> tr|A0A1X7SIB7|A0A1X7SIB7_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 110.4 0.0 2.9e-35 2.6e-31 259 376 .] 3 121 .. 1 121 [. 0.95 Alignments for each domain: == domain 1 score: 110.4 bits; conditional E-value: 2.9e-35 SB-TTT-SBEEEEEEEE-S-S--HHHHHHHHHHHCT..-TTS.-HHHHHHHHHHHHHHS--SS-EEHHHHHHHHT-SS- CS TRM 259 aecehcgaklhlaGPlwlgplkdkefveevleeaekekeeel.kkrvkklLklikeElldaplyydlsqlasvlklsvp 336 ++cehc+++++++GP+w +p++d f+ ++l ++++k++ + ++ ++L+++keEl ++p+++dlsql+svl+++v tr|A0A1X7SIB7|A0A1X7SIB7_AMPQE 3 KDCEHCSSNFKFGGPVWSDPIHDDIFISSLLSDLQETKDRFAtNSKMIGMLSMMKEELNNVPFFHDLSQLSSVLHCNVM 81 68**********************************8654446999********************************* PP --HHHHHHH....TT-EE-TTSTTEEE-SS-HHHHHHHHH CS TRM 337 plkkvvsalkelgfevsrtHakpkaikTnapleavvevlr 376 ++ +++sal + g+ vs +H++p+a+kT+ap ++v++++r tr|A0A1X7SIB7|A0A1X7SIB7_AMPQE 82 RMLEMRSALMNQGYGVSSSHTNPQAVKTDAPHSVVWDIMR 121 ***************************************7 PP >> tr|A0A1X7VCI4|A0A1X7VCI4_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 23.3 0.0 8.7e-09 7.6e-05 39 124 .. 122 209 .. 94 217 .. 0.83 Alignments for each domain: == domain 1 score: 23.3 bits; conditional E-value: 8.7e-09 .HH.HHHHHH-S-EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HHHHHC. CS TRM 39 qle.llkkklgekikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvineddAnvlla. 115 l+ l+++++ +k+ l+ +sG ++ ++E ++ + +a Di++kAvel keN+ nev++++ + ++ + l++ tr|A0A1X7VCI4|A0A1X7VCI4_AMPQE 122 ILNfLQNHHCRQKLDFLELCCGSGAISVSLLKE-NESLSGYATDILPKAVELTKENSIRNEVDQRLIVTKAPITDLMSd 199 333134456789*********************.8888999**********************9888888899999998 PP .--SS-EEEE CS TRM 116 .ekkkrfdvi 124 e ++rfd+i tr|A0A1X7VCI4|A0A1X7VCI4_AMPQE 200 tELESRFDFI 209 8889999998 PP >> tr|A0A1X7UNT6|A0A1X7UNT6_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.0 0.0 4.7e-05 0.41 309 366 .. 150 211 .. 127 216 .. 0.81 Alignments for each domain: == domain 1 score: 11.0 bits; conditional E-value: 4.7e-05 HHHHHHH.S--SS-EEH.HHHHHHHT-SS---HHHHHHH....TT-..EE-TTSTTEEE-SS CS TRM 309 klikeEl.ldaplyydl.sqlasvlklsvpplkkvvsalkelgfev..srtHakpkaikTna 366 ++++ E l++ ++y++ s+ +++l+ + pp++ + + l ++gf+v +++H++++ ik + tr|A0A1X7UNT6|A0A1X7UNT6_AMPQE 150 NTLTSEQlLESYFMYKIvSKASERLSEKYPPVSVLHDRLSKIGFRVqsVLAHCHESIIKMDT 211 455555448999999863788899********************9844689*******9986 PP >> tr|A0A1X7VVN5|A0A1X7VVN5_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.5 0.0 6.9e-05 0.6 261 327 .. 72 140 .. 19 153 .. 0.75 Alignments for each domain: == domain 1 score: 10.5 bits; conditional E-value: 6.9e-05 -TTT-SBEEEEEEEE-S-S--HHHHHHHHHHHCT....-TTS-HHHHHHHHHHHHHHS--SS-EEHHHH CS TRM 261 cehcgaklhlaGPlwlgplkdkefveevleeaekek..eeelkkrvkklLklikeElldaplyydlsql 327 + +++ ++ GP++ g + kef++ +++ ++ ++ ++ +++r+++l++ + +p ++++ tr|A0A1X7VVN5|A0A1X7VVN5_AMPQE 72 LTVLETCVKNCGPMFHGRIATKEFLKDLTNVIQPKNnpPTIVRERILGLIQYWADAFKANPELAAVNEV 140 45566777889******************9988765424445*******99887776666665555555 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (376 nodes) Target sequences: 43435 (14716620 residues searched) Passed MSV filter: 1486 (0.034212); expected 868.7 (0.02) Passed bias filter: 941 (0.0216646); expected 868.7 (0.02) Passed Vit filter: 69 (0.00158858); expected 43.4 (0.001) Passed Fwd filter: 5 (0.000115115); expected 0.4 (1e-05) Initial search space (Z): 43435 [actual number of targets] Domain search space (domZ): 5 [number of targets reported over threshold] # CPU time: 0.41u 0.01s 00:00:00.42 Elapsed: 00:00:00.13 # Mc/sec: 42564.99 // [ok]