# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/TRM.hmm.txt # target sequence database: proteomes/Dictyostelium_discoideum.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TRM [M=376] Accession: PF02005.15 Description: N2,N2-dimethylguanosine tRNA methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3e-125 417.8 0.2 4.1e-125 417.4 0.2 1.1 1 tr|Q54PG3|Q54PG3_DICDI tRNA (guanine(26)-N(2))-dimethyltrans 3.2e-06 26.1 0.1 6.4e-06 25.1 0.1 1.5 1 sp|Q54WD6|TRM5_DICDI tRNA (guanine(37)-N1)-methyltransfera ------ inclusion threshold ------ 0.016 13.9 0.3 0.027 13.2 0.3 1.4 1 sp|Q86JB0|Y8324_DICDI Putative uncharacterized protein DDB_ 0.068 11.8 0.0 0.1 11.3 0.0 1.2 1 tr|Q54PP5|Q54PP5_DICDI Uncharacterized protein OS=Dictyostel Domain annotation for each sequence (and alignments): >> tr|Q54PG3|Q54PG3_DICDI tRNA (guanine(26)-N(2))-dimethyltransferase OS=Dictyostelium discoideum OX=44689 GN=DDB018607 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 417.4 0.2 1.3e-128 4.1e-125 8 376 .] 57 455 .. 44 455 .. 0.91 Alignments for each domain: == domain 1 score: 417.4 bits; conditional E-value: 1.3e-128 ----SS--TT--SS--GGGHHHHHHHHHH....HHHHHHHH-S-......EEEEESS-TTSHHHHHHHHH-.S-EEEEEEE-S-HHH CS TRM 8 edkevtvskkeeVFYNPvqefnRDlsvlvir.qlellkkklgek......ikvldaLsasGlRaiRfalEv.pgvkevlaNDisekA 86 + +++ ++++eVFYNPvqefnRD+s+l+i+ ++e +kk++ e+ i+vl+aL+a+GlR+iR+a+E+ + +laNDi++ A tr|Q54PG3|Q54PG3_DICDI 57 NSATILYNNQNEVFYNPVQEFNRDMSILMIKlFIEERKKECLERgkpfkkIRVLEALAATGLRSIRYAKEIgDDLDYILANDILQTA 143 3334558899*********************9999999999887889999********************9446789********** PP HHHHHHHHHHTT--G.GEEEE-S-HHHHHC...--SS-EEEEEE--SS--GGGHHHHHHHEEEEEEEEEEE--HHHHTTSSHHHHHH CS TRM 87 velikeNvklnevee.ivvineddAnvlla...ekkkrfdviDlDPfGspapfldsavqsvkekglLlvTaTDtavLaGaspekclr 169 v++i +N ++n v e ++++n +dA+++++ +++k++dv+D+DP+G+p++fldsavq+v+++glL+vTaTDtavL+G++pe+c++ tr|Q54PG3|Q54PG3_DICDI 144 VDSIVKNREYNGVPEeRIKPNLGDATMVMMenrTHSKQYDVVDVDPYGAPTQFLDSAVQAVADGGLLCVTATDTAVLCGSYPEACFH 230 *************955**************8878899************************************************** PP HHSSB--.-STTHHHHHHHHHHHHHHHHHHTTTEEEEEEEEEEETTEEEEEEEEEE-HHHHHHHHTTEEEEEEETTT.......... CS TRM 170 kYgaval.kselcHEvalRillkavareaakYekavepllslsadfYvrvfvkvktgaakvkkvleklglvlhCsgC.......... 245 kY++v++ + +cHE++lRill++++++a++Y++ ++p+lsls+dfYvrvfv+v +++ ++kk l+k++ ++ C+gC tr|Q54PG3|Q54PG3_DICDI 231 KYQSVPVhRAGFCHEMGLRILLHSIEQHANRYKRHIVPILSLSVDFYVRVFVRVYSSPLEAKKALSKMANIFSCIGCgsfhiqptgv 317 ******934679*****************************************************************9888777664 PP .......--EEEE--GGG--.........SB-TTT-SBEEEEEEEE-S-S--HHHHHHHHHHHCT..-TTS..-HHHHHHHHHHHHH CS TRM 246 .......lseekvkglakfk.........aecehcgaklhlaGPlwlgplkdkefveevleeaekekeeel..kkrvkklLklikeE 314 + k+k +c+hc +++++aGP+w+ +++ +f ++ + +ek+ +++ ++r+ ++L+ + +E tr|Q54PG3|Q54PG3_DICDI 318 vkeegnsI---------KYKvpllnkevgSHCQHCTKQYQFAGPFWAKRIHNVDFCKQAISFLEKNP-TTFntSRRMYGVLTSVIDE 394 32222221.........223567777778************************************76.33336************** PP HS--SS-EEHHHHHHHHT-SS---HHHHHHH....TT-EE-TTSTTEEE-SS-HHHHHHHHH CS TRM 315 lldaplyydlsqlasvlklsvpplkkvvsalkelgfevsrtHakpkaikTnapleavvevlr 376 l+d+ +y++ ++ +++l+ls+pp+ + sa+++ g++vs +H+ p +ikT+ap++++++++r tr|Q54PG3|Q54PG3_DICDI 395 LPDQMFYFKSDHFTHTLHLSTPPTAMIKSAILNGGYKVSSSHVLP-SIKTDAPFQFLWDIFR 455 *******************************************99.6************986 PP >> sp|Q54WD6|TRM5_DICDI tRNA (guanine(37)-N1)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=trmt5 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 25.1 0.1 2e-09 6.4e-06 52 137 .. 216 302 .. 178 305 .. 0.81 Alignments for each domain: == domain 1 score: 25.1 bits; conditional E-value: 2e-09 EEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HHHHHC...--SS-EEEEEE--SS--GGGHH CS TRM 52 kvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvineddAnvlla...ekkkrfdviDlDPfGspapfld 137 + d +++ G a+ a+ ++ +v+aND+++ v+ +keN+k n e v+i++ dA+ +++ ek+ f ++ + ++ +fld sp|Q54WD6|TRM5_DICDI 216 IICDMFAGVGPFALPAAKI--KKCKVYANDLNPSSVKYMKENAKTNRLESKVEISNLDARDFVKslvEKSIPFTHVVMNLPSTSIEFLD 302 5677777777777777765..6779***********************************99987777777788877776666777766 PP >> sp|Q86JB0|Y8324_DICDI Putative uncharacterized protein DDB_G0277003 OS=Dictyostelium discoideum OX=44689 GN=DDB_G027 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 13.2 0.3 8.5e-06 0.027 51 115 .. 169 234 .. 60 246 .. 0.82 Alignments for each domain: == domain 1 score: 13.2 bits; conditional E-value: 8.5e-06 EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--..GGEEEE-S-HHHHHC CS TRM 51 ikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneve..eivvineddAnvlla 115 ++l+ s++Gl +i ++ k+v++ D s+k ++ +k N++ln+ e + ++ + dd n ++ sp|Q86JB0|Y8324_DICDI 169 KNILELGSGTGLVGIILD-FIKPLKKVILTDYSPKVLKNLKFNMELNNLEiqDFINDDDDDNNNNVN 234 5899999*******9655.4699************************99876777777777665554 PP >> tr|Q54PP5|Q54PP5_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0284401 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.3 0.0 3.2e-05 0.1 48 111 .. 49 110 .. 23 126 .. 0.85 Alignments for each domain: == domain 1 score: 11.3 bits; conditional E-value: 3.2e-05 -S-EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HH CS TRM 48 gekikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvineddAn 111 ++++++ld +++G ++ + Ev + ++ + D+s+k +e++ + vk n++++ ++++ +d + tr|Q54PP5|Q54PP5_DICDI 49 EKPMTILDVAAGTGPLSL-YGAEVYKNSKFITTDFSPKMIEIVDRLVKDNNIKN-IETHVMDGQ 110 45799*********9999.8999999999999*********************9.777777765 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (376 nodes) Target sequences: 12739 (6856946 residues searched) Passed MSV filter: 958 (0.0752021); expected 254.8 (0.02) Passed bias filter: 233 (0.0182903); expected 254.8 (0.02) Passed Vit filter: 16 (0.00125599); expected 12.7 (0.001) Passed Fwd filter: 4 (0.000313996); expected 0.1 (1e-05) Initial search space (Z): 12739 [actual number of targets] Domain search space (domZ): 4 [number of targets reported over threshold] # CPU time: 0.17u 0.01s 00:00:00.18 Elapsed: 00:00:00.06 # Mc/sec: 42970.19 // [ok]