# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/TRM.hmm.txt # target sequence database: proteomes/Nematostella_vectensis.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TRM [M=376] Accession: PF02005.15 Description: N2,N2-dimethylguanosine tRNA methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.3e-133 446.3 0.0 1.6e-133 446.0 0.0 1.1 1 tr|A7RIG7|A7RIG7_NEMVE tRNA (guanine(26)-N(2))-dimethyltrans 7.4e-19 68.6 0.0 9.6e-19 68.2 0.0 1.2 1 tr|A7T796|A7T796_NEMVE Predicted protein (Fragment) OS=Nemat 1.1e-05 25.2 0.0 1.9e-05 24.5 0.0 1.4 2 tr|A7SVE3|A7SVE3_NEMVE Predicted protein OS=Nematostella vec 1.5e-05 24.8 0.0 1.8e-05 24.5 0.0 1.2 1 tr|A7SVE2|A7SVE2_NEMVE Predicted protein OS=Nematostella vec 1.5e-05 24.8 0.0 4.7e-05 23.2 0.0 1.6 2 tr|A7SY10|A7SY10_NEMVE Predicted protein OS=Nematostella vec 2e-05 24.4 0.0 2.4e-05 24.1 0.0 1.1 1 tr|A7RYK6|A7RYK6_NEMVE Predicted protein OS=Nematostella vec 2e-05 24.4 0.0 2.4e-05 24.1 0.0 1.1 1 tr|A7T8E8|A7T8E8_NEMVE Predicted protein OS=Nematostella vec 2.4e-05 24.1 0.0 3.8e-05 23.5 0.0 1.4 1 tr|A7SY09|A7SY09_NEMVE Predicted protein OS=Nematostella vec 8.3e-05 22.4 0.0 0.00011 22.0 0.0 1.1 1 tr|A7RHA8|A7RHA8_NEMVE Predicted protein OS=Nematostella vec 0.00046 19.9 0.2 0.00064 19.4 0.2 1.2 1 tr|A7SHN7|A7SHN7_NEMVE Predicted protein (Fragment) OS=Nemat ------ inclusion threshold ------ 0.1 12.2 0.0 0.16 11.5 0.0 1.3 1 tr|A7SAF9|A7SAF9_NEMVE Predicted protein OS=Nematostella vec Domain annotation for each sequence (and alignments): >> tr|A7RIG7|A7RIG7_NEMVE tRNA (guanine(26)-N(2))-dimethyltransferase OS=Nematostella vectensis OX=45351 GN=v1g238527 P # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 446.0 0.0 7.2e-137 1.6e-133 1 376 [] 20 440 .. 20 440 .. 0.94 Alignments for each domain: == domain 1 score: 446.0 bits; conditional E-value: 7.2e-137 TTEEEEE----SS--TT--SS--GGGHHHHHHHHHH...HHHHHHHH-S-........................EEEEESS-TTSHH CS TRM 1 GeakikvedkevtvskkeeVFYNPvqefnRDlsvlvirqlellkkklgek........................ikvldaLsasGlR 63 G+++i++ ++e+++++++ VFYNPvqefnRD+s +vir + + k++++ +++l++L+asGlR tr|A7RIG7|A7RIG7_NEMVE 20 GKTRIREGKAEIIFPAEKAVFYNPVQEFNRDISSAVIRLVCEDIDKWTKRrndekgdadkltraekrakdnlaqVTILEGLAASGLR 106 88999999999***************************55555555555569999******************************** PP HHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HHHHHC....--SS-EEEEEE--SS--GGGHHHHHHHEEEE CS TRM 64 aiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvineddAnvlla....ekkkrfdviDlDPfGspapfldsavqsvkek 146 ++R+alEvpg ++v+aNDis A li++Nvk n+v++iv ++++dA++l++ ++rf +iDlDP+G++apfld avq+v+++ tr|A7RIG7|A7RIG7_NEMVE 107 SVRYALEVPGIHHVIANDISADAFSLIENNVKHNKVNHIVSASKNDASLLMYqyryPASRRFTFIDLDPYGTAAPFLDGAVQAVADG 193 ****************************************************8887789**************************** PP EEEEEEE--HHHHTTSSHHHHHHHHSSB---STTHHHHHHHHHHHHHHHHHHTTTEEEEEEEEEEETTEEEEEEEEEE-HHHHHHHH CS TRM 147 glLlvTaTDtavLaGaspekclrkYgavalkselcHEvalRillkavareaakYekavepllslsadfYvrvfvkvktgaakvkkvl 233 g+LlvT+TD+ vL+G+++++c+ kYg+++lk ++cHE+alR+ll++++++a++Y++++ plls+s+dfY+rvfv+v t+a +vk+++ tr|A7RIG7|A7RIG7_NEMVE 194 GVLLVTCTDMGVLCGNHNDACYGKYGGMSLKAKFCHEMALRLLLSSIESHANRYKRYILPLLSMSVDFYIRVFVQVFTSASEVKRTA 280 *************************************************************************************** PP TTEEEEEEETTT.......--EEEE--GGG--..........SB-TTT-SBEEEEEEEE-S-S--HHHHHHHHHHHCT..-TTS.-H CS TRM 234 eklglvlhCsgC.......lseekvkglakfk..........aecehcgaklhlaGPlwlgplkdkefveevleeaekekeeel.kk 302 +k+ lv+hC+gC + ++ ++g+ k k + c +cg k+hl+GP+w +p++d++fv+++++ ++++++ + tr|A7RIG7|A7RIG7_NEMVE 281 SKQSLVYHCTGCgsfhfqqVGRRIEEGVSK-KyppalgppvdRSCDQCGGKFHLGGPIWTEPMHDTDFVTSLAALVKQNTDLYKtSD 366 ************998877633344445444.24677888888***********************************98754335** PP HHHHHHHHHHHHHS--SS-EEHHHHHHHHT-SS---HHHHHHH....TT-EE-TTSTTEEE-SS-HHHHHHHHH CS TRM 303 rvkklLklikeElldaplyydlsqlasvlklsvpplkkvvsalkelgfevsrtHakpkaikTnapleavvevlr 376 r+ +lL+++ eEl+ +pl++++++l++v+++s+p+ +++sal++lg++vs++H++p+a+kT+ap +++++v+r tr|A7RIG7|A7RIG7_NEMVE 367 RIIGLLSMVAEELPTVPLFHTIDHLSNVIHCSSPSIVQFRSALLRLGYKVSGSHVCPTAVKTDAPNNVLWDVMR 440 *************************************************************************7 PP >> tr|A7T796|A7T796_NEMVE Predicted protein (Fragment) OS=Nematostella vectensis OX=45351 GN=v1g3263 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 68.2 0.0 4.3e-22 9.6e-19 304 376 .] 1 73 [. 1 73 [. 0.98 Alignments for each domain: == domain 1 score: 68.2 bits; conditional E-value: 4.3e-22 HHHHHHHHHHHHS--SS-EEHHHHHHHHT-SS---HHHHHHH....TT-EE-TTSTTEEE-SS-HHHHHHHHH CS TRM 304 vkklLklikeElldaplyydlsqlasvlklsvpplkkvvsalkelgfevsrtHakpkaikTnapleavvevlr 376 + +lL+++ eEl+ +pl++++++l++v+++s+p+ +++sal++lg++vs++H++p+a+kT+ap +++++v+r tr|A7T796|A7T796_NEMVE 1 ISGLLSMVAEELPTVPLFHTIDHLSNVIHCSSPSIVQFRSALLRLGYKVSGSHVCPTAVKTDAPNNVLWDVMR 73 679*********************************************************************7 PP >> tr|A7SVE3|A7SVE3_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g218064 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.8 0.0 1.6 3.6e+03 320 357 .. 5 40 .. 2 46 .. 0.74 2 ! 24.5 0.0 8.4e-09 1.9e-05 43 109 .. 67 132 .. 13 148 .. 0.81 Alignments for each domain: == domain 1 score: -2.8 bits; conditional E-value: 1.6 S-EEHHHHHHHHT-SS---HHHHHHH....TT-EE-TT CS TRM 320 lyydlsqlasvlklsvpplkkvvsalkelgfevsrtHa 357 ++d+++ +++++s+ +k+v +++ g+ vs t + tr|A7SVE3|A7SVE3_NEMVE 5 SFFDTNAAVEKMRISSHTKKRVT--INQRGYVVSPTVF 40 68888888888888888888774..6678888887755 PP == domain 2 score: 24.5 bits; conditional E-value: 8.4e-09 HHHHH-S-EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S- CS TRM 43 lkkklgekikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvinedd 109 +k+++++ +k l+ ++ G ai fal vp+ ev+a Di++ Ave + eN+ l+ ve+ v++ ++d tr|A7SVE3|A7SVE3_NEMVE 67 QKSNTESSLKFLEVGAGMGAIAIEFALQVPQ-AEVWATDINPAAVEDVAENACLHGVENSVTAIQAD 132 33334556999*****************886.58************************988877755 PP >> tr|A7SVE2|A7SVE2_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g218063 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 24.5 0.0 8.3e-09 1.8e-05 43 109 .. 67 132 .. 8 148 .. 0.80 Alignments for each domain: == domain 1 score: 24.5 bits; conditional E-value: 8.3e-09 HHHHH-S-EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S- CS TRM 43 lkkklgekikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvinedd 109 +k+++++ +k l+ ++ G ai fal vp+ ev+a Di++ Ave + eN+ l+ ve+ v++ ++d tr|A7SVE2|A7SVE2_NEMVE 67 QKSNTESSLKFLEVGAGMGAIAIEFALQVPQ-AEVWATDINPAAVEDVAENACLHGVENSVTAIQAD 132 33444566999*****************886.58************************988877755 PP >> tr|A7SY10|A7SY10_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g219300 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -1.2 0.0 0.55 1.2e+03 319 358 .. 4 41 .. 1 58 [. 0.81 2 ! 23.2 0.0 2.1e-08 4.7e-05 44 109 .. 68 132 .. 52 148 .. 0.80 Alignments for each domain: == domain 1 score: -1.2 bits; conditional E-value: 0.55 SS-EEHHHHHHHHT-SS---HHHHHHH....TT-EE-TTS CS TRM 319 plyydlsqlasvlklsvpplkkvvsalkelgfevsrtHak 358 ++d+++ +++++s++ +k+v +++ g+ vs t + tr|A7SY10|A7SY10_NEMVE 4 ISFFDTNAAVEKMRISSQTKKRVT--INQRGYVVSPTVFD 41 679999999999999999888875..67788888887665 PP == domain 2 score: 23.2 bits; conditional E-value: 2.1e-08 HHHH-S-EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S- CS TRM 44 kkklgekikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvinedd 109 k+++++ +k l+ ++ G ai fal vp+ ev+a Di++ Ave + eN+ l+ v++ v++ ++d tr|A7SY10|A7SY10_NEMVE 68 KSNTESSLKFLEVGAGMGAIAIEFALQVPQ-AEVWATDINPAAVEDVAENACLHGVANSVTAIQAD 132 33345569999999*************886.58************************988877755 PP >> tr|A7RYK6|A7RYK6_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g241579 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 24.1 0.0 1.1e-08 2.4e-05 43 110 .. 67 133 .. 18 154 .. 0.81 Alignments for each domain: == domain 1 score: 24.1 bits; conditional E-value: 1.1e-08 HHHHH-S-EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-H CS TRM 43 lkkklgekikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvineddA 110 +k+++++ +k l+ ++ G ai fal vp+ ev+a Di++ Av+ + eN++l+ ve v++ ++d tr|A7RYK6|A7RYK6_NEMVE 67 QKSNTESSLKFLEVGAGMGAIAIEFALQVPQ-AEVWATDINPAAVDDVAENARLHGVETSVTAIQADV 133 33444566999***9*************886.58************************9887777564 PP >> tr|A7T8E8|A7T8E8_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g223766 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 24.1 0.0 1.1e-08 2.4e-05 43 110 .. 67 133 .. 18 154 .. 0.81 Alignments for each domain: == domain 1 score: 24.1 bits; conditional E-value: 1.1e-08 HHHHH-S-EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-H CS TRM 43 lkkklgekikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvineddA 110 +k+++++ +k l+ ++ G ai fal vp+ ev+a Di++ Av+ + eN++l+ ve v++ ++d tr|A7T8E8|A7T8E8_NEMVE 67 QKSNTESSLKFLEVGAGMGAIAIEFALQVPQ-AEVWATDINPAAVDDVAENARLHGVETSVTAIQADV 133 33444566999***9*************886.58************************9887777564 PP >> tr|A7SY09|A7SY09_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g248028 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 23.5 0.0 1.7e-08 3.8e-05 50 109 .. 68 132 .. 10 148 .. 0.78 Alignments for each domain: == domain 1 score: 23.5 bits; conditional E-value: 1.7e-08 -......EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S- CS TRM 50 k......ikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvinedd 109 k +k l+ ++ G ai fal vp+ ev+a Di++ Ave + eN+ l+ ve+ v++ ++d tr|A7SY09|A7SY09_NEMVE 68 KsdtesfLKFLEVGAGMGAIAIEFALQVPQ-AEVWATDINPAAVEDVAENACLHGVENSVTAIQAD 132 033334489999999************886.58************************988877755 PP >> tr|A7RHA8|A7RHA8_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g197133 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 22.0 0.0 5e-08 0.00011 38 109 .. 60 131 .. 19 146 .. 0.88 Alignments for each domain: == domain 1 score: 22.0 bits; conditional E-value: 5e-08 ...HHHHHHHH-S-EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S- CS TRM 38 r.qlellkkklgekikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvinedd 109 r e ++kk ge + +l+ ++Gl +i al +++ +v+a D+++ Av+ eN+kl+ v++ v + ++d tr|A7RHA8|A7RHA8_NEMVE 60 RvLAEEMDKKPGEAFALLEIGCGTGLTSIMAALY-SKKCQVWATDVNPSAVKNTAENAKLHGVDNQVRTATGD 131 55567788999999******************98.9999*************************988887776 PP >> tr|A7SHN7|A7SHN7_NEMVE Predicted protein (Fragment) OS=Nematostella vectensis OX=45351 GN=v1g118817 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 19.4 0.2 2.9e-07 0.00064 55 137 .. 225 312 .. 169 315 .. 0.80 Alignments for each domain: == domain 1 score: 19.4 bits; conditional E-value: 2.9e-07 ESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HH.HHHC.......--SS-EEEEEE--SS--G CS TRM 55 daLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvineddAn.vlla.......ekkkrfdviDlDPfGspa 133 d +++ G ai a+ v+aND+++ + +++N+k n+v++++++ + d + ++++ e++k f+++ + ++ tr|A7SHN7|A7SHN7_NEMVE 225 DMFAGVGPFAIPAAKK---GCFVYANDLNPSSFKALEHNAKTNQVADRIKAFNLDGReFVMQvtentlkENNKMFNHVVMNLPATAL 308 5555555555555554...5679***********************97776666665155658888888889999999888878888 PP GGHH CS TRM 134 pfld 137 +fld tr|A7SHN7|A7SHN7_NEMVE 309 QFLD 312 8887 PP >> tr|A7SAF9|A7SAF9_NEMVE Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g243959 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 11.5 0.0 7.3e-05 0.16 53 114 .. 56 114 .. 28 132 .. 0.82 Alignments for each domain: == domain 1 score: 11.5 bits; conditional E-value: 7.3e-05 EEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HHHHH CS TRM 53 vldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvineddAnvll 114 ++da+ + G ai fa ++v+a Di + +++ ++N+++ v++++++ +d + ++ tr|A7SAF9|A7SAF9_NEMVE 56 IVDAFCGVGGNAIQFAFT---CERVIAIDIDPVKIDCARHNAEIYGVADRIEFVLGDFRQIV 114 89**************88...789999**********************9999988877666 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (376 nodes) Target sequences: 24428 (8257945 residues searched) Passed MSV filter: 784 (0.0320943); expected 488.6 (0.02) Passed bias filter: 505 (0.020673); expected 488.6 (0.02) Passed Vit filter: 39 (0.00159653); expected 24.4 (0.001) Passed Fwd filter: 11 (0.000450303); expected 0.2 (1e-05) Initial search space (Z): 24428 [actual number of targets] Domain search space (domZ): 11 [number of targets reported over threshold] # CPU time: 0.24u 0.01s 00:00:00.25 Elapsed: 00:00:00.07 # Mc/sec: 44356.96 // [ok]