# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/TRM.hmm.txt # target sequence database: proteomes/Strongylocentrotus_purpuratus.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TRM [M=376] Accession: PF02005.15 Description: N2,N2-dimethylguanosine tRNA methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6.7e-87 292.8 0.5 8.6e-87 292.4 0.5 1.0 1 tr|W4YDZ5|W4YDZ5_STRPU Uncharacterized protein OS=Strongyloc 9.5e-53 180.4 2.1 2.1e-42 146.3 0.2 3.1 3 tr|W4YN62|W4YN62_STRPU tRNA (guanine(26)-N(2))-dimethyltrans 3.2e-20 73.3 0.0 4.2e-20 72.9 0.0 1.2 1 tr|W4XT20|W4XT20_STRPU Uncharacterized protein OS=Strongyloc 4.8e-05 23.4 0.0 4.8e-05 23.4 0.0 1.0 1 tr|W4XMN7|W4XMN7_STRPU Uncharacterized protein OS=Strongyloc ------ inclusion threshold ------ 0.071 12.9 0.0 0.11 12.3 0.0 1.3 1 tr|W4YBQ4|W4YBQ4_STRPU Uncharacterized protein OS=Strongyloc 0.083 12.7 0.0 6.5 6.5 0.0 2.3 2 tr|W4YER5|W4YER5_STRPU tRNA (guanine(37)-N1)-methyltransfera 0.23 11.2 0.0 0.41 10.4 0.0 1.3 1 tr|W4Y0S2|W4Y0S2_STRPU Uncharacterized protein OS=Strongyloc Domain annotation for each sequence (and alignments): >> tr|W4YDZ5|W4YDZ5_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 292.4 0.5 2.1e-90 8.6e-87 44 270 .. 36 282 .. 20 284 .. 0.92 Alignments for each domain: == domain 1 score: 292.4 bits; conditional E-value: 2.1e-90 HHHH-S-EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HHHHHC..--SS-EEEEEE-- CS TRM 44 kkklgekikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvineddAnvlla..ekkkrfdviDlDP 128 ++k++e i+ l+aLsasGlR++R a+Evpg k+v+aND+se+Av i++N+++n+ve++v+++++dA++l++ + k f+v+DlDP tr|W4YDZ5|W4YDZ5_STRPU 36 GQKCEEGITFLEALSASGLRSVRIAKEVPGIKRVVANDFSEEAVRDIERNIQFNNVEDLVEASHSDASLLMYkhRFKDSFNVVDLDP 122 6899999*****************************************************************6666789******** PP SS--GGGHHHHHHHEEEEEEEEEEE--HHHHTTSSHHHHHHHHSSB---STTHHHHHHHHHHHHHHHHHHTTTEEEEEEEEEEETTE CS TRM 129 fGspapfldsavqsvkekglLlvTaTDtavLaGaspekclrkYgavalkselcHEvalRillkavareaakYekavepllslsadfY 215 +Gs+a+flds vq+v+++glL+vT+TD+avL+G++ ++c++kYga+al++++c E+alRill+ ++++a++Y+++++p+ls+++dfY tr|W4YDZ5|W4YDZ5_STRPU 123 YGSAAQFLDSGVQAVRNGGLLCVTCTDMAVLCGNHGHACFAKYGAMALRTKFCNEMALRILLQCIETQATRYNRYIVPVLSVAVDFY 209 *************************************************************************************** PP EEEEEEEEE-HHHHHHHHTTEEEEEEETTT............--EEEE--GGG--.................SB-TTT-SBEEE CS TRM 216 vrvfvkvktgaakvkkvleklglvlhCsgC............lseekvkglakfk.................aecehcgaklhl 270 vrvfv+v+ ++ kvk + +k +lv+hCsgC k + c+hc +++h+ tr|W4YDZ5|W4YDZ5_STRPU 210 VRVFVRVHISQSKVKYSPSKKALVYHCSGCgsfnlqrlgkvtF-----------KennpnpifqpatgppvdKSCQHCDSSFHV 282 ******************************8776555444332...........23334555557789999899****999997 PP >> tr|W4YN62|W4YN62_STRPU tRNA (guanine(26)-N(2))-dimethyltransferase OS=Strongylocentrotus purpuratus OX=7668 PE=3 SV= # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 5.7 0.1 0.0028 12 73 100 .. 15 42 .. 1 70 [. 0.67 2 ! 146.3 0.2 5.1e-46 2.1e-42 102 315 .. 132 349 .. 80 374 .. 0.87 3 ! 26.8 0.0 1e-09 4.3e-06 312 375 .. 399 461 .. 388 462 .. 0.86 Alignments for each domain: == domain 1 score: 5.7 bits; conditional E-value: 0.0028 -EEEEEEE-S-HHHHHHHHHHHHHTT-- CS TRM 73 gvkevlaNDisekAvelikeNvklneve 100 ev + Di+++ v +++N ln+ tr|W4YN62|W4YN62_STRPU 15 DDVEVTVSDINPESVRNMRRNCILNNLL 42 4568999******************976 PP == domain 2 score: 146.3 bits; conditional E-value: 5.1e-46 GEEEE-S-HHHHHC--SS-EEEEEE--SS--GGGHHHHHHHEEEEEEEEEEE--HHHHTTSSHHHHHHHHSSB---STTHHHHHHHH CS TRM 102 ivvineddAnvllaekkkrfdviDlDPfGspapfldsavqsvkekglLlvTaTDtavLaGaspekclrkYgavalkselcHEvalRi 188 +v+ dAn+l+ +++ fd+i lDP+G ++pf+dsa ++++g+L++T+TDta+L G+ p+ lr+Yg++ k e+ E a R+ tr|W4YN62|W4YN62_STRPU 132 TVTLVCCDANLLM--RQRFFDFIFLDPYGCATPFMDSAFSNISNNGILAITSTDTASLYGKCPQVTLRNYGGQVAKCEYLKELAGRL 216 5677778******..899********************************************************************* PP HHHHHHHHHHTTTEEEEEEEEEEETTEEEEEEEEEE-HHHHHHHHTTEEEEEEETTT--EEEE--GGG--........SB-T.TT-S CS TRM 189 llkavareaakYekavepllslsadfYvrvfvkvktgaakvkkvleklglvlhCsgClseekvkglakfk........aece.hcga 266 ++ a +r+aa+ +k +e+ll+++ +++v v ++v++g +++++++++ vlhC C +++ v ++ ++ c+ tr|W4YN62|W4YN62_STRPU 217 VVAAAVRAAARCNKGLEVLLCVALEHFVLVILRVRRGSGQADRSVQEVQHVLHCRVCGQRKFVPSTLTVPnsmydhlsCTCHkSAPC 303 *********************************************************666555554444444555544233202334 PP BEEEEEEEE-S-S--HHHHHHHHHHHCT..-TTS-HHHHHHHHHHHHHH CS TRM 267 klhlaGPlwlgplkdkefveevleeaekekeeelkkrvkklLklikeEl 315 + l GP+w g + d+ f+++++++ + +l+k+ ++lL+++ eE+ tr|W4YN62|W4YN62_STRPU 304 TAMLLGPVWSGSIFDAGFIDRMAHAGVE---LRLSKKDLQLLHTLLEEA 349 66789*****************999888...789999999999999996 PP == domain 3 score: 26.8 bits; conditional E-value: 1e-09 HHHHS--SS-EEHHHHHHHHT-SS---HHHHHHH....TT-EE-TTSTTEEE-SS-HHHHHHHH CS TRM 312 keElldaplyydlsqlasvlklsvpplkkvvsalkelgfevsrtHakpkaikTnapleavvevl 375 +e + +y++l q s+ +++ p+ k++++ l+ +g+++srtH+ p++++Tna l ++ vl tr|W4YN62|W4YN62_STRPU 399 DENQGQPAFYFSLPQ-HSIKNMDKPRIKTFIECLRMAGHRASRTHFDPEGVRTNAALANFKAVL 461 455566667777777.5678999*********************************99988877 PP >> tr|W4XT20|W4XT20_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 72.9 0.0 1e-23 4.2e-20 305 376 .] 2 73 .. 1 73 [. 0.98 Alignments for each domain: == domain 1 score: 72.9 bits; conditional E-value: 1e-23 HHHHHHHHHHHS--SS-EEHHHHHHHHT-SS---HHHHHHH....TT-EE-TTSTTEEE-SS-HHHHHHHHH CS TRM 305 kklLklikeElldaplyydlsqlasvlklsvpplkkvvsalkelgfevsrtHakpkaikTnapleavvevlr 376 k+++++++eEl+d plyy +s+l++v++++ p+++kv+sa++++g++vs +Ha+p+a+kT+ap++++++++r tr|W4XT20|W4XT20_STRPU 2 KGMMTVVSEELSDCPLYYITSHLCNVVHCECPSQEKVRSAILNAGYRVSISHASPEALKTDAPASVIWDIIR 73 79********************************************************************86 PP >> tr|W4XMN7|W4XMN7_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 23.4 0.0 1.2e-08 4.8e-05 270 315 .. 1 47 [. 1 67 [] 0.77 Alignments for each domain: == domain 1 score: 23.4 bits; conditional E-value: 1.2e-08 EEEEEE-S-S--HHHHHHHHHHHCT...-TTS-HHHHHHHHHHHHHH CS TRM 270 laGPlwlgplkdkefveevleeaekek.eeelkkrvkklLklikeEl 315 ++GP+w++p++d fv++++ +++ + ++k r+k+++++++e tr|W4XMN7|W4XMN7_STRPU 1 VGGPIWADPIHDVGFVKRLITHIDTYPdRFKYKDRMKGMMTVVSEVR 47 58********************99965344559********998863 PP >> tr|W4YBQ4|W4YBQ4_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.3 0.0 2.8e-05 0.11 51 122 .. 518 585 .. 442 611 .. 0.80 Alignments for each domain: == domain 1 score: 12.3 bits; conditional E-value: 2.8e-05 EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HHHHHC--SS-EE CS TRM 51 ikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvineddAnvllaekkkrfd 122 ++ld ++G +i a+ v++v+ +++e+A+e keN+ +n v++ v+++ + A+ ll +++r + tr|W4YBQ4|W4YBQ4_STRPU 518 TTLLDICCGTGTIGISLAKR---VEKVIGVEMNEQAIEDAKENAAINGVDN-VTFHCGKAEDLLPGITNRLH 585 57899999999999999998...6677777899*****************9.99999999999985555444 PP >> tr|W4YER5|W4YER5_STRPU tRNA (guanine(37)-N1)-methyltransferase OS=Strongylocentrotus purpuratus OX=7668 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 6.5 0.0 0.0016 6.5 53 112 .. 241 297 .. 152 305 .. 0.84 2 ? 3.7 0.0 0.011 44 76 112 .. 437 473 .. 429 481 .. 0.87 Alignments for each domain: == domain 1 score: 6.5 bits; conditional E-value: 0.0016 EEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HHH CS TRM 53 vldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvineddAnv 112 v d +++ G ai a+ ev aND++++ ++ N+k n+ + ++++++ d + tr|W4YER5|W4YER5_STRPU 241 VYDMFAGVGPFAIPAAKK---GCEVHANDLNPESFRWLEVNAKQNKLSSRLKASNLDGRQ 297 667778888778877776...6689***********************999988878765 PP == domain 2 score: 3.7 bits; conditional E-value: 0.011 EEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HHH CS TRM 76 evlaNDisekAvelikeNvklneveeivvineddAnv 112 ev aND++++ ++ N+k n+ + ++++++ d + tr|W4YER5|W4YER5_STRPU 437 EVHANDLNPESFRWLELNAKQNKLSSRLKASNLDGRQ 473 799**********************999988878765 PP >> tr|W4Y0S2|W4Y0S2_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.4 0.0 0.0001 0.41 52 108 .. 1295 1348 .. 1242 1377 .. 0.86 Alignments for each domain: == domain 1 score: 10.4 bits; conditional E-value: 0.0001 EEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S CS TRM 52 kvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvined 108 ++da+ +sG ai fa ++v+a Di + +el ++N+++ ve+++++ + tr|W4Y0S2|W4Y0S2_STRPU 1295 LIVDAFCGSGGNAIQFAFT---CERVVAVDIDPAKIELARHNATVYGVEDRIEFIVG 1348 489**************88...789999*********************98887654 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (376 nodes) Target sequences: 28594 (14266044 residues searched) Passed MSV filter: 980 (0.0342729); expected 571.9 (0.02) Passed bias filter: 690 (0.0241309); expected 571.9 (0.02) Passed Vit filter: 69 (0.00241309); expected 28.6 (0.001) Passed Fwd filter: 7 (0.000244807); expected 0.3 (1e-05) Initial search space (Z): 28594 [actual number of targets] Domain search space (domZ): 7 [number of targets reported over threshold] # CPU time: 0.40u 0.01s 00:00:00.41 Elapsed: 00:00:00.13 # Mc/sec: 41261.79 // [ok]