# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/TRM.hmm.txt # target sequence database: proteomes/Trichoplax_adhaerens.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TRM [M=376] Accession: PF02005.15 Description: N2,N2-dimethylguanosine tRNA methyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-110 369.6 6.3 4.1e-95 318.5 4.3 2.0 2 tr|B3RPX0|B3RPX0_TRIAD tRNA (guanine(26)-N(2))-dimethyltrans ------ inclusion threshold ------ 0.011 14.4 0.1 0.048 12.2 0.0 2.0 2 tr|B3S2Q2|B3S2Q2_TRIAD Uncharacterized protein OS=Trichoplax Domain annotation for each sequence (and alignments): >> tr|B3RPX0|B3RPX0_TRIAD tRNA (guanine(26)-N(2))-dimethyltransferase OS=Trichoplax adhaerens OX=10228 GN=TRIADDRAFT_53 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 318.5 4.3 7.1e-99 4.1e-95 8 268 .. 6 301 .. 1 304 [. 0.92 2 ! 52.3 0.0 5.5e-18 3.2e-14 313 376 .] 302 365 .. 299 365 .. 0.97 Alignments for each domain: == domain 1 score: 318.5 bits; conditional E-value: 7.1e-99 ----SS--TT--SS--GGGHHHHHHHHHH....HHHHHHHH-S-..............EEEEESS-TTSHHHHHHHHH-S-EEEEEE CS TRM 8 edkevtvskkeeVFYNPvqefnRDlsvlvir.qlellkkklgek..............ikvldaLsasGlRaiRfalEvpgvkevla 79 e+++++++++++ FYNP+q fnRD+s++vi+ ++++ +k+++++ ++++++Lsa+GlR+iR+alE+p+ k+v+a tr|B3RPX0|B3RPX0_TRIAD 6 EGQAKILNDDQDAFYNPIQTFNRDISIAVIKqFIKTCQKEVSTQckvrrnlsaavekgFTIFEGLSATGLRSIRYALELPKIKAVIA 92 444588999*********************978888888887779****************************************** PP E-S-HHHHHHHHHHHHHTT--GGEEEE-S-HHHHHC....--SS-EEEEEE--SS--GGGHHHHHHHEEEEEEEEEEE--HHHHTTS CS TRM 80 NDisekAvelikeNvklneveeivvineddAnvlla....ekkkrfdviDlDPfGspapfldsavqsvkekglLlvTaTDtavLaGa 162 ND ++ ++i++N+++n++ ++v+++ +dA+++++ + + +f+v+D+DP+Gspa fld+avqsv+++glL++T+TD+avL+G+ tr|B3RPX0|B3RPX0_TRIAD 93 NDNNPSSFKCIERNIEYNKIGHVVQPHFGDARMMMYdnsnSLDDQFQVVDIDPYGSPAIFLDAAVQSVSDGGLLCITCTDMAVLCGN 179 ************************************887777889****************************************** PP SHHHHHHHHSSB---STTHHHHHHHHHHHHHHHHHHTTTEEEEEEEEEEETTEEEEEEEEEE-HHHHHHHHTTEEEEEEETTT.... CS TRM 163 spekclrkYgavalkselcHEvalRillkavareaakYekavepllslsadfYvrvfvkvktgaakvkkvleklglvlhCsgC.... 245 + +c++kYg+v+l+s+ cHE+alRi l++++++a++Y ++++plls+s+d+Y+rvfv+v g+++v+++++k +v++C++C tr|B3RPX0|B3RPX0_TRIAD 180 HAGSCFAKYGSVSLRSKHCHEMALRIALSSIESNANRYGRYIVPLLSVSVDYYIRVFVRVFYGKTEVNRSASKKSFVFQCTSCdsfy 266 *************************************************************************************** PP .......--EEEE--GGG--.....SB-TTT-SBE CS TRM 246 .......lseekvkglakfk.....aecehcgakl 268 ++++++++++ k ++c++c +kl tr|B3RPX0|B3RPX0_TRIAD 267 lqplgskADTGNNQNFQPAKfnmenQKCTECDSKL 301 99988773334444444444557779999998876 PP == domain 2 score: 52.3 bits; conditional E-value: 5.5e-18 HHHS--SS-EEHHHHHHHHT-SS---HHHHHHH....TT-EE-TTSTTEEE-SS-HHHHHHHHH CS TRM 313 eElldaplyydlsqlasvlklsvpplkkvvsalkelgfevsrtHakpkaikTnapleavvevlr 376 +E +d+plyy+++ la+ +++ p k++sal++lg++vs tHa+++a+kT+a+ ++v++v+r tr|B3RPX0|B3RPX0_TRIAD 302 KEIPDQPLYYVTDSLANSIHSVCIPFLKIRSALLQLGYKVSSTHAHKSAFKTDASNSVVWDVMR 365 6889***********************************************************7 PP >> tr|B3S2Q2|B3S2Q2_TRIAD Uncharacterized protein OS=Trichoplax adhaerens OX=10228 GN=TRIADDRAFT_58107 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -1.6 0.5 0.13 7.3e+02 245 327 .. 124 205 .. 118 218 .. 0.70 2 ? 12.2 0.0 8.3e-06 0.048 44 109 .. 549 618 .. 512 647 .. 0.81 Alignments for each domain: == domain 1 score: -1.6 bits; conditional E-value: 0.13 T--EEEE--GGG--SB-TTT-SBEEEEE.EEE-S-S--HHHHHHHHHHHCT..-TTS-HHHHHHHHHHHHHHS--SS-EEHHHH CS TRM 245 ClseekvkglakfkaecehcgaklhlaG.PlwlgplkdkefveevleeaekekeeelkkrvkklLklikeElldaplyydlsql 327 C+s++++ +l+++++e e + + ++ +G P g k++ e +++ ++ k+++ kk+++++ + ++e+ da l+ d ++l tr|B3S2Q2|B3S2Q2_TRIAD 124 CTSSQSEDNLNTVQNEDESLMQLMQTMGlPTAFGSNKQNSNYEDQARKSHSGKKKKGKKKINRMKEEANED--DANLMTDNHHL 205 77777777777777777777777777776777777666666666666667767777788888888887777..66666565555 PP == domain 2 score: 12.2 bits; conditional E-value: 8.3e-06 HHHH-S-.......EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S- CS TRM 44 kkklgek.......ikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklneveeivvinedd 109 +k++++ ++da+ + G i fa+ +v+a Di ++ +++ ++N+k+ +ve+ +++ +d tr|B3S2Q2|B3S2Q2_TRIAD 549 PEKIAQHiaercrcSIIIDAFCGVGGNCIQFAKT---CDHVIAIDIDPNKIKCARHNAKIYNVEHKIEFIVGD 618 444444444455545699**************98...6789999********************988766554 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (376 nodes) Target sequences: 11518 (5222075 residues searched) Passed MSV filter: 459 (0.0398507); expected 230.4 (0.02) Passed bias filter: 267 (0.0231811); expected 230.4 (0.02) Passed Vit filter: 18 (0.00156277); expected 11.5 (0.001) Passed Fwd filter: 2 (0.000173641); expected 0.1 (1e-05) Initial search space (Z): 11518 [actual number of targets] Domain search space (domZ): 2 [number of targets reported over threshold] # CPU time: 0.15u 0.00s 00:00:00.15 Elapsed: 00:00:00.05 # Mc/sec: 39270.00 // [ok]