# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.1b2 (February 2015); http://hmmer.org/
# Copyright (C) 2015 Howard Hughes Medical Institute.
# Freely distributed under the GNU General Public License (GPLv3).
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: pfam_list/TRM.hmm.txt
# target sequence database: proteomes/Trypanosoma_brucei.fasta
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: TRM [M=376]
Accession: PF02005.15
Description: N2,N2-dimethylguanosine tRNA methyltransferase
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
8.6e-102 340.1 0.1 1.2e-71 240.8 0.0 2.9 2 tr|Q386C5|Q386C5_TRYB2 N(2), N(2)-dimethylguanosine tRNA met
0.00092 17.4 0.0 0.0012 17.0 0.0 1.2 1 tr|Q389Z0|Q389Z0_TRYB2 Uncharacterized protein OS=Trypanosom
------ inclusion threshold ------
0.032 12.3 0.0 0.047 11.8 0.0 1.2 1 tr|Q583H8|Q583H8_TRYB2 Nucleolar protein, putative OS=Trypan
Domain annotation for each sequence (and alignments):
>> tr|Q386C5|Q386C5_TRYB2 N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative OS=Trypanosoma brucei brucei (s
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 94.9 0.0 9.4e-31 2.7e-27 18 100 .. 163 252 .. 149 265 .. 0.92
2 ! 240.8 0.0 4.3e-75 1.2e-71 116 375 .. 336 683 .. 312 684 .. 0.88
Alignments for each domain:
== domain 1 score: 94.9 bits; conditional E-value: 9.4e-31
--SS--GGGHHHHHHHHHH....HHHHHHHH-S-......EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHT CS
TRM 18 eeVFYNPvqefnRDlsvlvir.qlellkkklgek......ikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvkln 97
+ VFYNP+q +nRDlsv vi +++l+k++ ++k i++l+aLsa+GlRaiR+ +E+p v+ ++aNDi Ave+i++N ++n
tr|Q386C5|Q386C5_TRYB2 163 QAVFYNPAQVVNRDLSVCVIAcFSQLRKEEPKNKggtrrgITILEALSATGLRAIRYYKEIPDVRFIIANDIDCDAVECIRRNCEFN 249
67******************989999999988889**************************************************** PP
T-- CS
TRM 98 eve 100
ev
tr|Q386C5|Q386C5_TRYB2 250 EVP 252
*97 PP
== domain 2 score: 240.8 bits; conditional E-value: 4.3e-75
--SS-EEEEEE--SS--GGGHHHHHHHEEEEEEEEEEE--HHHHTTSSHHHHHHHHSSB---STTHHHHHHHHHHHHHHHHHHTTTE CS
TRM 116 ekkkrfdviDlDPfGspapfldsavqsvkekglLlvTaTDtavLaGaspekclrkYgavalkselcHEvalRillkavareaakYek 202
+++ dv+DlDP+Gs++pfl+ av++++e+glLlvT+TD+a L+G++p++c +kY++v++k+ +cHE+a+Rill av+r a+k +k
tr|Q386C5|Q386C5_TRYB2 336 IQQELVDVVDLDPYGSASPFLEGAVRCIREGGLLLVTSTDSAILCGNYPDTCHAKYNTVPTKNAACHEMAVRILLAAVERVANKHRK 422
467778********************************************************************************* PP
EEEEEEEEEETTEEEEEEEEEE-HHHHHHHHTTEEEEEEETTT............................................ CS
TRM 203 avepllslsadfYvrvfvkvktgaakvkkvleklglvlhCsgC............................................ 245
+++pllsl +dfYvr fv+v t++a+vk + klg++++C +C
tr|Q386C5|Q386C5_TRYB2 423 YIVPLLSLHIDFYVRCFVRVYTQPAEVKLSPCKLGYLIQCNHCpafwvrqiavsrmrvkkrsrklgnagedvtagsqgnvakseehr 509
*****************************************************9999999999999999999998888888777777 PP
.....................--EEEE--GGG--...............................SB-TTT-SBEEEEEEEE-S-S- CS
TRM 246 .....................lseekvkglakfk...............................aecehcgaklhlaGPlwlgplk 280
+ c+ cga+++l GP++++p +
tr|Q386C5|Q386C5_TRYB2 510 lsdsgnargtneggeahkadeW-----------GwerfpaapsrrenpkivspslqqifpsslrgHCCCVCGASVSLSGPIYAAPTQ 585
7666666665555555555550...........0555555555555555666667888888999999******************** PP
-HHHHHHHHHHHCT..-TTS...-HHHHHHHHHHHHHHS--SS-EEHHHHHHHHT-SS---HHHHHHH....TT-EE-TTSTTEEE- CS
TRM 281 dkefveevleeaekekeeel...kkrvkklLklikeElldaplyydlsqlasvlklsvpplkkvvsalkelgfevsrtHakpkaikT 364
++ f++++l e+e + + + r+ +l +++ eEl d+pl+y+l ++as ++++ pp ++ al + g+++s+ H+ p++ikT
tr|Q386C5|Q386C5_TRYB2 586 SAPFLGQLLVEIERRASANHitaVARISGLVRVAMEELADTPLFYQLPDVASFVRVRCPPAPLFIGALGRKGYRCSQVHCAPSGIKT 672
********99999876333336789************************************************************** PP
SS-HHHHHHHH CS
TRM 365 napleavvevl 375
+ p e vv v+
tr|Q386C5|Q386C5_TRYB2 673 DCPPEIVVGVM 683
*******9998 PP
>> tr|Q389Z0|Q389Z0_TRYB2 Uncharacterized protein OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 17.0 0.0 4.3e-07 0.0012 38 101 .. 199 261 .. 141 280 .. 0.87
Alignments for each domain:
== domain 1 score: 17.0 bits; conditional E-value: 4.3e-07
...HHHHHHHH-S-EEEEESS-TTSHHHHHHHHH-S-EEEEEEE-S-HHHHHHHHHHHHHTT--G CS
TRM 38 r.qlellkkklgekikvldaLsasGlRaiRfalEvpgvkevlaNDisekAvelikeNvklnevee 101
r +l+ + +++++vld ++G +i a+ vp++ +v a Di+++A+ +eN+kln e
tr|Q389Z0|Q389Z0_TRYB2 199 RtWLSKIPET-TQPLRVLDMCCGTGCVGIAIAKHVPSA-QVTAVDILDEAIGASEENAKLNGLEA 261
5455555444.799********************8875.7999******************9883 PP
>> tr|Q583H8|Q583H8_TRYB2 Nucleolar protein, putative OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 G
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 11.8 0.0 1.7e-05 0.047 28 127 .. 192 332 .. 170 348 .. 0.75
Alignments for each domain:
== domain 1 score: 11.8 bits; conditional E-value: 1.7e-05
HHHHHHHHH....HHHHHHHH-S-..........................................EEEEESS-TTSHHHHHHHHH- CS
TRM 28 fnRDlsvlvir.qlellkkklgek..........................................ikvldaLsasGlRaiRfalEv 71
RDl+ ++++ +++ + +k +vld +a G ++ a+ +
tr|Q583H8|Q583H8_TRYB2 192 KRRDLVQALVKrGMNVEPLEKWSKvglqvfesnvpisgtveylaghymlqsavsflpvmalapqinERVLDMAAAPGGKTTYIAQLM 278
5556555555444444433222224566666666666666666666666666666666666666556899999*******999*99* PP
S-EEEEEEE-S-HHHHHHHHHHHHHTT--GGEEEE-S-HHHHHC--SS-EEEEEE- CS
TRM 72 pgvkevlaNDisekAvelikeNvklneveeivvineddAnvllaekkkrfdviDlD 127
+++ ++aND+se ++++ N + v ++vv+n d + +++ k+fd i lD
tr|Q583H8|Q583H8_TRYB2 279 KNTGVIFANDVSEPRTKSLNANLQRLGVTNTVVTNY-DGVGF-EKVMKNFDRILLD 332
******************************999999.66554.5888899998888 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (376 nodes)
Target sequences: 8561 (4332363 residues searched)
Passed MSV filter: 225 (0.026282); expected 171.2 (0.02)
Passed bias filter: 178 (0.020792); expected 171.2 (0.02)
Passed Vit filter: 17 (0.00198575); expected 8.6 (0.001)
Passed Fwd filter: 3 (0.000350426); expected 0.1 (1e-05)
Initial search space (Z): 8561 [actual number of targets]
Domain search space (domZ): 3 [number of targets reported over threshold]
# CPU time: 0.12u 0.01s 00:00:00.13 Elapsed: 00:00:00.05
# Mc/sec: 32579.37
//
[ok]