# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/TRM13.hmm.txt # target sequence database: proteomes/Dictyostelium_discoideum.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TRM13 [M=262] Accession: PF05206.13 Description: Methyltransferase TRM13 Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.7e-78 261.1 6.8 1.9e-77 259.9 6.8 1.7 1 sp|Q54BD5|TRM13_DICDI tRNA:m(4)X modification enzyme TRM13 0.0022 17.1 1.3 0.0033 16.5 1.3 1.2 1 tr|Q54N63|Q54N63_DICDI Uncharacterized protein OS=Dictyostel ------ inclusion threshold ------ 0.12 11.4 2.5 0.17 10.9 1.4 1.9 2 tr|Q86I59|Q86I59_DICDI Methyltransferase-like protein OS=Dic 4.6 6.2 15.3 1.8 7.5 1.0 2.7 3 sp|Q7KWX1|BLML1_DICDI Beta-lactamase-like protein 1 OS=Dict Domain annotation for each sequence (and alignments): >> sp|Q54BD5|TRM13_DICDI tRNA:m(4)X modification enzyme TRM13 homolog OS=Dictyostelium discoideum OX=44689 GN=trm13 PE= # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 259.9 6.8 5.9e-81 1.9e-77 1 261 [. 252 523 .. 252 524 .. 0.95 Alignments for each domain: == domain 1 score: 259.9 bits; conditional E-value: 5.9e-81 TRM13 1 lqqsslignleelkllekssayvEfGaGkgelsryvnqaleedkkenskyvLidrasnrl..kaDrklrkdeselaikRlkidIkdLn 86 +q+ss+i+ le+++l++++++y+EfGaG g+ls+++ +++e++ s ++Lidr++ r k+Dr +++++ +++++Rl idI++Ln sp|Q54BD5|TRM13_DICDI 252 QQESSIINLLENSNLYNSDNVYLEFGAGSGKLSNHIFMSHEKK----SGHILIDRMKFRSlkKVDRLIKNEKGCHHFSRLLIDIRHLN 335 599*************************************666....9***********888************************** PP TRM13 87 lsaleelekkkkvvavsKHLCGaATDLtLrcllnsekask...kakleglliAlCChhvCswkeyvnkeyleel.gitkeefqiltkm 170 ls+l+ le +k++v++sKHLCG+ATD+tL++++n ++s+ +++++g+ iA+CChh+C++++y n++yl+++ +it efq+++++ sp|Q54BD5|TRM13_DICDI 336 LSNLSILE-EKPFVITSKHLCGCATDFTLDSIFNLLNNSNekvNNNFKGIGIATCCHHICNFNTYSNQSYLKDQlNITPLEFQLICSI 422 ******98.***********************999999888889**************************87544************* PP TRM13 171 vSWavsgkrkse..........aeeedekeekekeekkeeeeseeekksklsseereeiGlkakrlidegRllalkekgfeaelvkYv 248 +SWa+ +++k++ ++e +k++kek+e +++ + e +k++ +s++++ee+G+kakrlid+gR+l++kek ++ ++++ sp|Q54BD5|TRM13_DICDI 423 SSWATIDENKCNnddddneveeDDELIKKDKKEKKEFENQLKIEFNKQDVFSIKRKEELGYKAKRLIDYGRYLFIKEKLNLPNTKFWI 510 *********9999****99988777788999999999*************************************************** PP TRM13 249 ekevslEnvlLla 261 ++ s+En++L+ sp|Q54BD5|TRM13_DICDI 511 YTNQSKENLFLVS 523 **********986 PP >> tr|Q54N63|Q54N63_DICDI Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0186522 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 16.5 1.3 1e-06 0.0033 135 207 .. 38 106 .. 23 144 .. 0.62 Alignments for each domain: == domain 1 score: 16.5 bits; conditional E-value: 1e-06 TRM13 135 iAlCChhvCswkeyvnkeyleelgitkeefqiltkmvSWavsgkrkse.....aeeedekeekekeekkeeeeseeek 207 + CC h++++key++++ + ++ q+++ S + +++++++ + +++++++++++++++++++ tr|Q54N63|Q54N63_DICDI 38 FVFCCAHASQIKEYIQSSRWH--DL--PGVQVICMTGSNCNNNNNNNNnnnnnN-----NNNNNNNNNNNNNNNNNNN 106 558************966633..33..356899988898888887666444331.....2222222222222222222 PP >> tr|Q86I59|Q86I59_DICDI Methyltransferase-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB0167391 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.6 0.0 0.7 2.2e+03 249 260 .. 89 100 .. 83 109 .. 0.70 2 ? 10.9 1.4 5.2e-05 0.17 181 248 .. 241 317 .. 210 330 .. 0.73 Alignments for each domain: == domain 1 score: -2.6 bits; conditional E-value: 0.7 TRM13 249 ekevslEnvlLl 260 +kevs+En+lL tr|Q86I59|Q86I59_DICDI 89 DKEVSKENQLLA 100 667777777764 PP == domain 2 score: 10.9 bits; conditional E-value: 5.2e-05 TRM13 181 seaeeedekeekekeekkeeeeseeekksklsseereeiGlkakrlidegRllalkek.........gfeaelvkYv 248 +++++++++++++++++++++++++++ ++++ +++++G d R+ ++ + gf++e Y+ tr|Q86I59|Q86I59_DICDI 241 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNTINGGSKNKLGDNFHVRFDGTRAYYFSLEvmeslykekGFKTEQNHYI 317 225555556666777777888888899999***************9*****99876554444444446666666666 PP >> sp|Q7KWX1|BLML1_DICDI Beta-lactamase-like protein 1 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0276787 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 6.6 3.0 0.0011 3.4 155 210 .. 219 274 .. 204 288 .. 0.53 2 ? -2.1 0.1 0.51 1.6e+03 181 198 .. 377 394 .. 359 406 .. 0.46 3 ? 7.5 1.0 0.00058 1.8 165 212 .. 478 524 .. 458 527 .. 0.66 Alignments for each domain: == domain 1 score: 6.6 bits; conditional E-value: 0.0011 TRM13 155 eelgitkeefqiltkmvSWavsgkrkseaeeedekeekekeekkeeeeseeekksk 210 +e +++ f++l k+ +s+++++++++++++++++++++++++++++++ ++ sp|Q7KWX1|BLML1_DICDI 219 SEVHYSNLGFALLGKILGEIISNSTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 274 56666777788888877777777775553333333333333333333333332222 PP == domain 2 score: -2.1 bits; conditional E-value: 0.51 TRM13 181 seaeeedekeekekeekk 198 ++++++d+++++++++++ sp|Q7KWX1|BLML1_DICDI 377 NNNNKNDNNNNNNENNDN 394 222222222222222222 PP == domain 3 score: 7.5 bits; conditional E-value: 0.00058 TRM13 165 qiltkmvSWavsgkrkseaeeedekeekekeekkeeeeseeekkskls 212 qil+ + + +++k++++ ee++e+ee++++e+++++++++++++ ls sp|Q7KWX1|BLML1_DICDI 478 QILIPVYEYLLNKKQEEK-EEKEEEEENQQDESQQQQQQQQNNIKLLS 524 677777777777777444.44445666666666667777777776665 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (262 nodes) Target sequences: 12739 (6856946 residues searched) Passed MSV filter: 4369 (0.342963); expected 254.8 (0.02) Passed bias filter: 1153 (0.0905095); expected 254.8 (0.02) Passed Vit filter: 214 (0.0167988); expected 12.7 (0.001) Passed Fwd filter: 28 (0.00219797); expected 0.1 (1e-05) Initial search space (Z): 12739 [actual number of targets] Domain search space (domZ): 4 [number of targets reported over threshold] # CPU time: 0.65u 0.01s 00:00:00.66 Elapsed: 00:00:00.19 # Mc/sec: 9455.37 // [ok]