# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/TruD.hmm.txt # target sequence database: proteomes/Anolis_carolinensis.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TruD [M=416] Accession: PF01142.17 Description: tRNA pseudouridine synthase D (TruD) Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9.4e-74 248.7 0.0 8.7e-69 232.4 0.0 2.4 2 tr|G1KQ00|G1KQ00_ANOCA Pseudouridylate synthase 7 OS=Anolis 2.2e-70 237.6 0.0 5.7e-67 226.4 0.0 2.2 2 tr|G1KBG1|G1KBG1_ANOCA Pseudouridylate synthase 7 like OS=An ------ inclusion threshold ------ 0.35 9.7 2.6 0.49 9.2 0.4 1.9 2 tr|H9GR00|H9GR00_ANOCA Uncharacterized protein OS=Anolis car Domain annotation for each sequence (and alignments): >> tr|G1KQ00|G1KQ00_ANOCA Pseudouridylate synthase 7 OS=Anolis carolinensis OX=28377 GN=PUS7 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 13.9 0.1 3.1e-06 0.019 2 32 .. 105 135 .. 104 152 .. 0.88 2 ! 232.4 0.0 1.4e-72 8.7e-69 39 416 .] 243 633 .. 231 633 .. 0.93 Alignments for each domain: == domain 1 score: 13.9 bits; conditional E-value: 3.1e-06 TruD 2 lgielyvtdtegigGrlrespeDFiVeEiee 32 +gi +v+ +g+ G l+e++ DF+V+Ei + tr|G1KQ00|G1KQ00_ANOCA 105 VGITKFVSTYKGFSGILKERYSDFVVHEIGK 135 899**************************54 PP == domain 2 score: 232.4 bits; conditional E-value: 1.4e-72 TruD 39 akegeylvlrvekrnwdtldlvrelaralgisrkrvgfaGtKDkrAvTtQlisirkvepeelekl..eikdvelevvgrarrklrlG 123 +++g+y+ + + k+n dt+d+++ l++ l+++ + ++++GtKDkrA+T+Q i++ ++++++l++l + + +l ++ ++++l+lG tr|G1KQ00|G1KQ00_ANOCA 243 KSRGSYCHFVLYKENKDTMDAINMLSKFLRVKPNIFSYMGTKDKRAITVQEIAVLRITAQRLAHLnkCLMNFKLGNFSYKNHPLKLG 329 578***********************************************************9996668****************** PP TruD 124 dLaGNkFeirvrdvaeeeeaakraeeileelkekggvPNyfGvQRFGsrrpvthlvGkaivkgdleeAveayvgkpfeeesedtrea 210 +L+GN+F++++r+++ ++ +++++ +++l+e g + Ny+G+QRFG++ t+ vG+ai++++++e v+ ++ +e+ + tr|G1KQ00|G1KQ00_ANOCA 330 ELQGNHFTVVLRNITGTD---EQVQHAMQSLREIG-FINYYGMQRFGTTAIPTYQVGRAILQNNWNEVVDLILKPRPGAEKGYLVKC 412 *************95445...59**********99.***********************************99999999******** PP TruD 211 reavaetgdleealeelpkslryEramlkrLae.kpkdykkAlealpknLqrlFvhAyqSylFNrvlseRlerglpldepveGDvvi 296 re a+t+d ++al++lp + +E ++l+ L + k++ +A+ +p+n + +++h+yqSy++N+++s+R+e++ ++v+GD+v+ tr|G1KQ00|G1KQ00_ANOCA 413 REEWAKTKDPAAALRKLPVKRCVEGQLLRGLSKyGLKNIVSAFGIIPRNNRLMYIHSYQSYVWNNMVSKRIEEY-G-LKAVPGDLVL 497 *********************************66799**********************************99.4.48*******9 PP TruD 297 faeerlpdtdrlqlvteknveevkrliergrafvtaplvGtetelaegevgeierevleeegleledFklpklpelsskGtrRaill 383 + + ++e+++++++ +++pl+G+++ ++ + ge +e+l+ ++l+ ++ k +k+ + s +G++R++++ tr|G1KQ00|G1KQ00_ANOCA 498 KGGTA-------TYIEESEADNYT------IHDIVMPLPGFDVIYPKHKIGEAYKEILAADNLDINNMK-HKIRDYSLSGAYRKVII 570 99555.......888888888888......44689**********************************.5689************* PP TruD 384 rve..............................dlelsaeedeltleFsLpkGsYATvvlrEi 416 r++ l+++ + + l+++FsLp+ +YAT+ +rE+ tr|G1KQ00|G1KQ00_ANOCA 571 RPQhvswelvayddpkiplfttdldkmegktpaVLATEGKYKALKMDFSLPPSTYATMAIREV 633 **************************99998764445555666******************96 PP >> tr|G1KBG1|G1KBG1_ANOCA Pseudouridylate synthase 7 like OS=Anolis carolinensis OX=28377 GN=PUS7L PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 9.0 0.0 9.3e-05 0.58 5 33 .. 14 42 .. 11 55 .. 0.85 2 ! 226.4 0.0 9.3e-71 5.7e-67 37 416 .] 292 693 .. 277 693 .. 0.86 Alignments for each domain: == domain 1 score: 9.0 bits; conditional E-value: 9.3e-05 TruD 5 elyvtdtegigGrlrespeDFiVeEieef 33 ++yv d+ g+ G +++ p DF+V+Ei+ tr|G1KBG1|G1KBG1_ANOCA 14 STYVNDHVGFWGCIKTFPSDFVVTEINMH 42 68************************543 PP == domain 2 score: 226.4 bits; conditional E-value: 9.3e-71 TruD 37 eeakegeylvlrvekrnwdtldlvrelaralgisrkrvgfaGtKDkrAvTtQlisirkvepeeleklei....kdvelevvgrarrk 119 +++ke y+ ++++k+n +tl++++ l+++lg+ +++++ G+KDk+AvT+Q + ++kv+p++l++l i k++ l+++++ +++ tr|G1KBG1|G1KBG1_ANOCA 292 NREKEEIYTAFTLQKENLETLEAISYLSSELGVLPSDFSYTGIKDKKAVTYQAMVVKKVTPKRLKELGIiiggKRLGLSNIHSVNQS 378 35677788***********************************************************777888************** PP TruD 120 lrlGdLaGNkFeirvrdv.aeee....eaakraeeileelkekggvPNyfGvQRFGsrrpv.thlvGkaivkgdleeAveayvgkpf 200 lrlG+L+GN+F+i+vrd+ +++ ++++r+ e +e++k++g + Ny+G QRFG+ + + t +G a++++++ +Av+ +++ tr|G1KBG1|G1KBG1_ANOCA 379 LRLGQLQGNHFAIIVRDLkLHSNdchgNLKERVYEAMEKVKKNG-FINYYGPQRFGQGQNIhTDQIGLALLNEEMVKAVKLFFTPED 464 *****************976666567667788888888888887.************88654889***************9998765 PP TruD 201 eeesedtreareavaetgdleealeelpkslryEramlkrLae...kpkdykkAlealpknLqrlFvhAyqSylFNrvlseRlergl 284 + ++ +++a++++ et+d++ l lp+ Er++l++L + +++ + ++p+ ++ ++vhAy S ++N+++s R++ + tr|G1KBG1|G1KBG1_ANOCA 465 S--DDPVNKAKKYFLETEDAKGTLAMLPDFKVRERMLLRALNRyglNHEGCIQGWLSIPHSMRIFYVHAYCSKIWNEAVSYRIKTY- 548 4..455679**********************************77777777777889***************************99. PP TruD 285 pldepveGDvvifaeer..lpdtdrlqlvteknveevkrliergrafvtaplvGtetelaegevgeierevleeegleledFklpkl 369 +++v+GD+v+ e++ +d+++++t+++ + + +v++p+ G++++ + +vge +e l+++gl++ +F+lp+ tr|G1KBG1|G1KBG1_ANOCA 549 -GTSVVTGDLVFSIEDNesCSPSDKVHVITSEEEMAN----KYTINQVIIPMAGYSVQYPTNKVGEWYHERLARDGLQMGHFRLPA- 629 .469*******99888754555556666655433333....334457999***********************************8. PP TruD 370 pelsskGtrRaillrve.................dlelsaeede...ltleFsLpkGsYATvvlrEi 416 +l++ G +R+il ++ + +++ + l ++F L++ +YATv l Ei tr|G1KBG1|G1KBG1_ANOCA 630 LQLNVPGCYRHILKYPHdlsysflenngkdltpgGDCFQD---SifsLSVSFWLDPSCYATVCLGEI 693 99***********999978888888887766554222333...25559**************99885 PP >> tr|H9GR00|H9GR00_ANOCA Uncharacterized protein OS=Anolis carolinensis OX=28377 GN=brinp3 PE=4 SV=2 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 0.0 0.1 0.048 3e+02 254 271 .. 167 184 .. 147 185 .. 0.84 2 ? 9.2 0.4 7.9e-05 0.49 204 268 .. 228 292 .. 219 310 .. 0.89 Alignments for each domain: == domain 1 score: 0.0 bits; conditional E-value: 0.048 TruD 254 alpknLqrlFvhAyqSyl 271 lp+ Lq+ Fv+A+ Sy+ tr|H9GR00|H9GR00_ANOCA 167 ILPEYLQERFVQAALSYI 184 59**************97 PP == domain 2 score: 9.2 bits; conditional E-value: 7.9e-05 TruD 204 sedtreareavaetgdleealeelpkslryEramlkrLaekpkdykkAlealpknLqrlFvhAyq 268 se++++ + ++++++++ ++++p ++ + + + +L + + ++++ e+l++ +++lF++A + tr|H9GR00|H9GR00_ANOCA 228 SETWNTYNNEFEDSDEFKFFMKRMPMNYFLNTSAIMHLWTMDSNFQHRYEQLETSMKQLFLKAQR 292 6778888899999*************************************************965 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (416 nodes) Target sequences: 18528 (9796552 residues searched) Passed MSV filter: 774 (0.0417746); expected 370.6 (0.02) Passed bias filter: 344 (0.0185665); expected 370.6 (0.02) Passed Vit filter: 36 (0.00194301); expected 18.5 (0.001) Passed Fwd filter: 3 (0.000161917); expected 0.2 (1e-05) Initial search space (Z): 18528 [actual number of targets] Domain search space (domZ): 3 [number of targets reported over threshold] # CPU time: 0.28u 0.01s 00:00:00.29 Elapsed: 00:00:00.09 # Mc/sec: 45281.84 // [ok]