# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.1b2 (February 2015); http://hmmer.org/
# Copyright (C) 2015 Howard Hughes Medical Institute.
# Freely distributed under the GNU General Public License (GPLv3).
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  pfam_list/TruD.hmm.txt
# target sequence database:        proteomes/Strongylocentrotus_purpuratus.fasta
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TruD  [M=416]
Accession:   PF01142.17
Description: tRNA pseudouridine synthase D (TruD)
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------               -----------
    1.4e-65  222.4   0.0      4e-54  184.7   0.0    3.1  3  tr|W4XQB7|W4XQB7_STRPU  Uncharacterized protein OS=Strongyloc
    5.4e-22   78.9   0.0    7.4e-07   29.0   0.0    4.0  4  tr|W4ZCS6|W4ZCS6_STRPU  Uncharacterized protein OS=Strongyloc
    4.2e-07   29.8   0.0    4.2e-07   29.8   0.0    1.0  1  tr|W4ZCL2|W4ZCL2_STRPU  Uncharacterized protein OS=Strongyloc
  ------ inclusion threshold ------
      0.017   14.6   0.0      0.018   14.5   0.0    1.1  1  tr|W4XUW1|W4XUW1_STRPU  Uncharacterized protein OS=Strongyloc
      0.027   14.0   0.2       0.04   13.4   0.2    1.2  1  tr|W4XM82|W4XM82_STRPU  Uncharacterized protein OS=Strongyloc
       0.24   10.8   0.0         23    4.3   0.0    2.2  2  tr|W4Y7B3|W4Y7B3_STRPU  Uncharacterized protein OS=Strongyloc


Domain annotation for each sequence (and alignments):
>> tr|W4XQB7|W4XQB7_STRPU  Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !   17.7   0.0   4.2e-07     0.002       2      31 ..       2      31 ..       1      43 [. 0.91
   2 !  184.7   0.0   8.3e-58     4e-54      45     388 ..     316     680 ..     290     699 .. 0.86
   3 !   16.3   0.0   1.1e-06    0.0053     387     416 .]     853     882 ..     829     882 .. 0.84

  Alignments for each domain:
  == domain 1  score: 17.7 bits;  conditional E-value: 4.2e-07
                    TruD  2 lgielyvtdtegigGrlrespeDFiVeEie 31
                            +gie+yv+++eg+ G++++sp+DF V+Ei+
  tr|W4XQB7|W4XQB7_STRPU  2 FGIECYVSQHEGFKGNVKTSPKDFQVTEIS 31
                            69**************************94 PP

  == domain 2  score: 184.7 bits;  conditional E-value: 8.3e-58
                    TruD  45 lvlrvekrnwdtldlvrelaralgisrkrvgfaGtKDkrAvTtQlisirkvepeelekl..eikd.velevvgrarrklrlGdLaGN 128
                             + ++++k+n +tl+++++lar +g++ +++++aG+KDk+AvTtQ +++++++  +l+++  e+++ +++ ++++ ++++ +G+L+GN
  tr|W4XQB7|W4XQB7_STRPU 316 TGFTLQKTNTETLSAIQQLARLMGVQISDFTYAGIKDKKAVTTQEMAVKGITITRLQDVskELPGwLKVGSIHSRNKSMVTGELWGN 402
                             66899**************************************************9999777765489******************* PP

                    TruD 129 kFeirvrdv.aeee..............eaakraeeileelkekggvPNyfGvQRFGsr.....rpvthl....vGkaivkgdleeA 191
                             +F+i+++++ +++               e+++++e++++ ++++g + Ny+G QRFG+      +  t +    vG a+++g+++eA
  tr|W4XQB7|W4XQB7_STRPU 403 HFDITLQNIeMKNGsnevragtvedkgrELQNCIEQCIRCVEDRG-FINYYGEQRFGASsvdpsKALTTIspadVGCAMLQGKYKEA 488
                             ********9554447888888888888888888888888888877.***********86222223333324444************* PP

                    TruD 192 veayvg...kpfeeesedtreareavaetgdleealeelpkslryEramlkrLae...kpkdykkAlealpknLqrlFvhAyqSylF 272
                             ++ +++    p+ + s+ t+ a++ +++tg  +eal+++p   + E  +lk L +   +++   kAl ++p+n+++++vhA+ S ++
  tr|W4XQB7|W4XQB7_STRPU 489 IDLILSpddGPCADPSNATNVAKHCFQQTGSPREALKQMPTMKTRECLLLKGLNRhgtDDDGCIKALMNIPHNMRQMYVHAFCSLVW 575
                             *****8333478999999*************************************55444448999********************* PP

                    TruD 273 NrvlseRlerglpldepveGDvvifaeerlpdtdrlqlvteknveevkrliergrafvtaplvGtetelaegevgeierevleeegl 359
                             N++ s R+ r+    ++veGD+v+ ++      +++++vte+ + + k         v++pl+G + + ++  +g+  ++ l+e+gl
  tr|W4XQB7|W4XQB7_STRPU 576 NQMASWRMCRY--GCQVVEGDLVCIEDLG----TKVHAVTEDDITQGK----YSLTEVMLPLPGNNIQYPSHTAGAEYQRRLDEAGL 652
                             ********999..459**********888....888889888888777....5556799**************************** PP

                    TruD 360 eledFklpklpelsskGtrRaillrvedl 388
                                +F++p+  +l++ G +R+++ ++++l
  tr|W4XQB7|W4XQB7_STRPU 653 GECNFRIPA-LKLNVPGGYRKVIAQPRNL 680
                             ********8.9***********9999854 PP

  == domain 3  score: 16.3 bits;  conditional E-value: 1.1e-06
                    TruD 387 dlelsaeedeltleFsLpkGsYATvvlrEi 416
                             + +ls+++++l l+F Lp  +YATv+lrE+
  tr|W4XQB7|W4XQB7_STRPU 853 SSSLSCQRKTLNLSFDLPASCYATVFLREL 882
                             5678899999******************96 PP

>> tr|W4ZCS6|W4ZCS6_STRPU  Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !   17.4   0.0   5.2e-07    0.0025       2      33 ..     159     190 ..     158     226 .. 0.86
   2 !   29.0   0.0   1.6e-10   7.4e-07      40      93 ..     291     344 ..     278     363 .. 0.88
   3 !   25.3   0.0     2e-09   9.5e-06     253     301 ..     369     415 ..     350     427 .. 0.83
   4 ?    2.7   0.0     0.015        73     346     388 ..     417     458 ..     415     460 .] 0.85

  Alignments for each domain:
  == domain 1  score: 17.4 bits;  conditional E-value: 5.2e-07
                    TruD   2 lgielyvtdtegigGrlrespeDFiVeEieef 33 
                             +gi  y++d+eg+ G +++++ DF+V+Ei e 
  tr|W4ZCS6|W4ZCS6_STRPU 159 VGIIEYMSDHEGVPGIIKQRYRDFLVHEIDEQ 190
                             79999************************655 PP

  == domain 2  score: 29.0 bits;  conditional E-value: 1.6e-10
                    TruD  40 kegeylvlrvekrnwdtldlvrelaralgisrkrvgfaGtKDkrAvTtQlisir 93 
                             ++ +y+ + + k+n+d +++++ +a+ l+i  + +++aG KDkr+vT+Q ++  
  tr|W4ZCS6|W4ZCS6_STRPU 291 ETPKYCRFVLFKENMDLNEALNIVAKYLHIGPHHFQYAGSKDKRGVTVQEVTAH 344
                             566899999****************************************98754 PP

  == domain 3  score: 25.3 bits;  conditional E-value: 2e-09
                    TruD 253 ealpknLqrlFvhAyqSylFNrvlseRlerglpldepveGDvvifaeer 301
                             +++p+n + +++h+yqSy++N +  +Rl+r     +pv GD+v  ++ +
  tr|W4ZCS6|W4ZCS6_STRPU 369 HSIPRNTRLIYLHSYQSYIWNVITTQRLQRD-G-FTPVVGDLVSAKNIQ 415
                             579**************************99.4.48*******988655 PP

  == domain 4  score: 2.7 bits;  conditional E-value: 0.015
                    TruD 346 vgeierevleeegleledFklpklpelsskGtrRaillrvedl 388
                             +ge   e l++++l+ e+ k ++  +ls +G++R+ +++++d+
  tr|W4ZCS6|W4ZCS6_STRPU 417 AGEWYTEELAKDDLTVEMLKNTN-RDLSLSGAYRKCVVKPTDV 458
                             5777788899**********554.6*************99876 PP

>> tr|W4ZCL2|W4ZCL2_STRPU  Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !   29.8   0.0   8.8e-11   4.2e-07     142     186 ..      11      54 ..       2      64 .] 0.82

  Alignments for each domain:
  == domain 1  score: 29.8 bits;  conditional E-value: 8.8e-11
                    TruD 142 eaakraeeileelkekggvPNyfGvQRFGsrrpvthlvGkaivkg 186
                               ++++++ +++l++ g + NyfG+QRFG+    t+ vG ++  +
  tr|W4ZCL2|W4ZCL2_STRPU  11 GSKEQINHAMSSLRDAG-FINYFGMQRFGNSLIATYHVGSYVTVN 54 
                             346799999*****988.*********************998655 PP

>> tr|W4XUW1|W4XUW1_STRPU  Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ?   14.5   0.0   3.9e-06     0.018     395     416 .]      63      84 ..      43      84 .. 0.88

  Alignments for each domain:
  == domain 1  score: 14.5 bits;  conditional E-value: 3.9e-06
                    TruD 395 deltleFsLpkGsYATvvlrEi 416
                             + l+ eFsLp+ +YAT+ lrE+
  tr|W4XUW1|W4XUW1_STRPU  63 SALKAEFSLPSSCYATMALREV 84 
                             56899****************6 PP

>> tr|W4XM82|W4XM82_STRPU  Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ?   13.4   0.2   8.4e-06      0.04     182     284 ..     224     331 ..     213     361 .. 0.84

  Alignments for each domain:
  == domain 1  score: 13.4 bits;  conditional E-value: 8.4e-06
                    TruD 182 aivkg.dleeAveayvgkpfeeesedtreareavaetgdleealeelpkslryEr..amlkrLae..kpkdykkAlealpknLqrlF 263
                             +++++  +e   ++y++ p+++e+++t+   + + e    ++  ++ p + + Er  a +++L++  +  + ++ l++l+k+L ++ 
  tr|W4XM82|W4XM82_STRPU 224 HLLQRaAIETEHRIYISLPSDDEHQNTHPLGQSLVECFPPKKQQRLTPIQQTAERcrAKIDSLVQllDVCNNQRILRELEKQLDDML 310
                             55544146677899****************99999988888888888877777752267888888777889999************* PP

                    TruD 264 vhAyqSylFNrvlseRlergl 284
                             v   qS+ F++  +eRl++g 
  tr|W4XM82|W4XM82_STRPU 311 VMMKQSMEFEKNTKERLNKGR 331
                             ******************984 PP

>> tr|W4Y7B3|W4Y7B3_STRPU  Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ?    4.3   0.0    0.0048        23     158     172 ..     102     114 ..      86     134 .. 0.81
   2 ?    3.9   0.0    0.0062        30     158     172 ..     178     190 ..     163     197 .. 0.81

  Alignments for each domain:
  == domain 1  score: 4.3 bits;  conditional E-value: 0.0048
                    TruD 158 ggvPNyfGvQRFGsr 172
                             g  PN+fG+QR G +
  tr|W4Y7B3|W4Y7B3_STRPU 102 G--PNFFGIQRLGMQ 114
                             3..**********85 PP

  == domain 2  score: 3.9 bits;  conditional E-value: 0.0062
                    TruD 158 ggvPNyfGvQRFGsr 172
                             g  PN+fG+QR G +
  tr|W4Y7B3|W4Y7B3_STRPU 178 G--PNFFGIQRLGMQ 190
                             3..*********975 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                              1  (416 nodes)
Target sequences:                        28594  (14266044 residues searched)
Passed MSV filter:                      1052  (0.0367909); expected 571.9 (0.02)
Passed bias filter:                      522  (0.0182556); expected 571.9 (0.02)
Passed Vit filter:                        49  (0.00171365); expected 28.6 (0.001)
Passed Fwd filter:                         6  (0.000209834); expected 0.3 (1e-05)
Initial search space (Z):              28594  [actual number of targets]
Domain search space  (domZ):               6  [number of targets reported over threshold]
# CPU time: 0.42u 0.01s 00:00:00.43 Elapsed: 00:00:00.14
# Mc/sec: 42390.53
//
[ok]