# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/UPF0020.hmm.txt # target sequence database: proteomes/Amphimedon_queenslandica.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: UPF0020 [M=197] Accession: PF01170.17 Description: Putative RNA methylase family UPF0020 Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6.6e-12 46.8 0.1 8e-11 43.2 0.1 2.3 1 tr|A0A1X7UZY0|A0A1X7UZY0_AMPQE Uncharacterized protein OS=Amphi 2.8e-05 25.2 0.0 0.00015 22.8 0.0 2.2 1 tr|A0A1X7UDY4|A0A1X7UDY4_AMPQE Uncharacterized protein OS=Amphi 3.5e-05 24.8 0.1 7.2e-05 23.8 0.1 1.5 1 tr|A0A1X7TED2|A0A1X7TED2_AMPQE Uncharacterized protein OS=Amphi 5e-05 24.3 0.1 0.00026 22.0 0.0 2.0 3 tr|A0A1X7VCI4|A0A1X7VCI4_AMPQE Uncharacterized protein OS=Amphi 0.00025 22.0 0.0 0.00049 21.1 0.0 1.4 1 tr|A0A1X7U0X0|A0A1X7U0X0_AMPQE Uncharacterized protein OS=Amphi 0.00028 21.9 0.0 0.00041 21.3 0.0 1.2 1 tr|A0A1X7SDI4|A0A1X7SDI4_AMPQE Uncharacterized protein OS=Amphi ------ inclusion threshold ------ 0.019 15.9 0.1 0.055 14.4 0.1 1.7 1 tr|A0A1X7SY39|A0A1X7SY39_AMPQE Uncharacterized protein OS=Amphi 0.13 13.2 0.1 0.28 12.1 0.0 1.5 2 tr|A0A1X7SEZ0|A0A1X7SEZ0_AMPQE Uncharacterized protein OS=Amphi Domain annotation for each sequence (and alignments): >> tr|A0A1X7UZY0|A0A1X7UZY0_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 43.2 0.1 1.5e-14 8e-11 3 136 .. 190 318 .. 189 331 .. 0.81 Alignments for each domain: == domain 1 score: 43.2 bits; conditional E-value: 1.5e-14 SS-SS---SS-HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEE CS UPF0020 3 yRefngpapLketLAaalvrlagwkpgepllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklyg 81 R+f g++++ + L+ ++ ++a++ pg+ +DP+ GsG+ilI a g ++ + + ++ + + + tr|A0A1X7UZY0|A0A1X7UZY0_AMPQE 190 TRQFIGNTSMDPLLSLVMANMAQVSPGKLVYDPFAGSGSILIACAHYGGYVLG-------SDIDWTIIHGRGKSSRSSS 261 699*************************************************3.......3344445555445555555 PP EES-HHHHHHHHHHHHHTT-GGG.EEEEE--GGG--.STTS---EEEE--------- CS UPF0020 82 sDldrrvvqgareNaekagvgdl.iefsqadaakLr.lkegevdvivtnpPYGerlg 136 + + r + r+N++++ ++++ i++ + d+ + ++++ +d+ivt+pPYG+r g tr|A0A1X7UZY0|A0A1X7UZY0_AMPQE 262 AGKWRGRHESVRNNLSQYSLEHQyIDMMVSDVSNSVwREKEIIDAIVTDPPYGIREG 318 666666678889********97548888888887666677789***********965 PP >> tr|A0A1X7UDY4|A0A1X7UDY4_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 22.8 0.0 2.7e-08 0.00015 48 132 .. 821 879 .. 789 883 .. 0.79 Alignments for each domain: == domain 1 score: 22.8 bits; conditional E-value: 2.7e-08 HHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STTS---EEE CS UPF0020 48 llganiapgllrefvlelkaeaeeearaelklygsDldrrvvqgareNaekagvgdliefsqadaakLrlkegevdviv 126 +++++D+d+ +++ar Nae +gv+d+ief d ++ + vdv+ tr|A0A1X7UDY4|A0A1X7UDY4_AMPQE 821 YTC--------------------------ERVIAIDIDPVKIACARHNAEIYGVADRIEFILGDYFDIMPHLKCVDVVF 873 333..........................3377**********************************9944448***** PP E----- CS UPF0020 127 tnpPYG 132 pP+G tr|A0A1X7UDY4|A0A1X7UDY4_AMPQE 874 ISPPWG 879 *****9 PP >> tr|A0A1X7TED2|A0A1X7TED2_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 23.8 0.1 1.3e-08 7.2e-05 79 134 .. 87 144 .. 55 152 .. 0.91 Alignments for each domain: == domain 1 score: 23.8 bits; conditional E-value: 1.3e-08 EEEEES-HHHHHHHHHHHHHTT-G.GGEEEEE--GGG--.STTS---EEEE------- CS UPF0020 79 lygsDldrrvvqgareNaekagvg.dliefsqadaakLr.lkegevdvivtnpPYGer 134 + ++D+d++ v+ +++Na+ g++ d+ ++ q d++ + k + d+++ npP+G + tr|A0A1X7TED2|A0A1X7TED2_AMPQE 87 VTAIDIDPNCVKLTQSNANDLGISsDQLSVIQCDIKTIDtDKFDRADTVIMNPPFGTK 144 789*******************963899***********777889***********75 PP >> tr|A0A1X7VCI4|A0A1X7VCI4_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -3.2 0.0 2.4 1.3e+04 110 128 .. 39 58 .. 31 59 .. 0.61 2 ? -1.1 0.1 0.58 3.1e+03 34 44 .. 139 149 .. 125 157 .. 0.80 3 ! 22.0 0.0 4.8e-08 0.00026 73 131 .. 154 215 .. 149 218 .. 0.92 Alignments for each domain: == domain 1 score: -3.2 bits; conditional E-value: 2.4 --GGG--.STTS---EEEE- CS UPF0020 110 adaakLr.lkegevdvivtn 128 d+ + + +e vd i++n tr|A0A1X7VCI4|A0A1X7VCI4_AMPQE 39 HDIEDYKlSAEYLVDYIICN 58 444444422566899***99 PP == domain 2 score: -1.1 bits; conditional E-value: 0.58 ETT-TTSHHHH CS UPF0020 34 DPmCGsGtilI 44 + CGsG+i + tr|A0A1X7VCI4|A0A1X7VCI4_AMPQE 139 ELCCGSGAISV 149 556****9975 PP == domain 3 score: 22.0 bits; conditional E-value: 4.8e-08 HHHH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--...STTS---EEEE---- CS UPF0020 73 araelklygsDldrrvvqgareNaekagvgdliefsqadaakLr...lkegevdvivtnpPY 131 +++ l+ y+ D+ +++v+ ++eN + +v+++ + +a +++L + e+ +d iv+npPY tr|A0A1X7VCI4|A0A1X7VCI4_AMPQE 154 ENESLSGYATDILPKAVELTKENSIRNEVDQRLIVTKAPITDLMsdtELESRFDFIVSNPPY 215 55677789***********************************988888889********** PP >> tr|A0A1X7U0X0|A0A1X7U0X0_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 21.1 0.0 9e-08 0.00049 79 134 .. 87 144 .. 59 154 .. 0.91 Alignments for each domain: == domain 1 score: 21.1 bits; conditional E-value: 9e-08 EEEEES-HHHHHHHHHHHHHTT-G.GGEEEEE--GGG--.STTS---EEEE------- CS UPF0020 79 lygsDldrrvvqgareNaekagvg.dliefsqadaakLr.lkegevdvivtnpPYGer 134 + ++D+d++ v+ +++Na+ g++ d+ ++ q d++ + + + d+++ npP+G + tr|A0A1X7U0X0|A0A1X7U0X0_AMPQE 87 VTAIDIDPNCVKLTQSNANDLGISsDQLSVIQYDIKTIDtDQFDRADTVIMNPPFGTK 144 789*******************963899***********777789***********75 PP >> tr|A0A1X7SDI4|A0A1X7SDI4_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 21.3 0.0 7.6e-08 0.00041 3 40 .. 100 137 .. 99 141 .. 0.95 Alignments for each domain: == domain 1 score: 21.3 bits; conditional E-value: 7.6e-08 SS-SS---SS-HHHHHHHHHHTT--TTS-EEETT-TTS CS UPF0020 3 yRefngpapLketLAaalvrlagwkpgepllDPmCGsG 40 R+f g++++ + L+ ++ ++a++ pg+ +DP+ GsG tr|A0A1X7SDI4|A0A1X7SDI4_AMPQE 100 TRQFIGNTSMDPLLSLVMANMAQVSPGKLVYDPFAGSG 137 699**********************************9 PP >> tr|A0A1X7SY39|A0A1X7SY39_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 14.4 0.1 1e-05 0.055 31 179 .. 440 607 .. 435 618 .. 0.68 Alignments for each domain: == domain 1 score: 14.4 bits; conditional E-value: 1e-05 -EEETT-TTSHH...HHHHHHHHTT--TTTT----SS................HHHHHHHHHH.....HH--.EEEEES CS UPF0020 31 pllDPmCGsGti...lIEaallganiapgllrefvle................lkaeaeeear.....aelk.lygsDl 84 +l+P+ G+Gt+ l+ +l++ + ++ ++++ + + ++++ + g++l tr|A0A1X7SY39|A0A1X7SY39_AMPQE 440 NILEPFAGTGTFiarLLQSGLIKPEDLERKYENEIFAneiqllpyyislinieS------AYQvasgdKNYRsFKGLNL 512 6899*******9555577888888777666655555599998888876543221......2222223433334777776 PP .-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STTS---EEEE----..------HHHHHHHHHHHHHHHHHH-TT.. CS UPF0020 85 .drrvvqgareNaekagvgdliefsqadaakLrlkegevdvivtnpPY..GerlgskkalekLYseflrelkrvlrggg 160 d ++++++ + + + e q + ++ + +dvi++npPY G+r ++ +a + Y+++ ++++ + +g+ tr|A0A1X7SY39|A0A1X7SY39_AMPQE 513 tDTFMIDTSEVKKLDLLRQYFEETHQ-RRKRME--KRRIDVILGNPPYsvGQRSQNDNAQNVSYPKLNARIHETYVKGS 588 23445666555555544444444444.333344..447**********888999999999999**********999988 PP -EEEEEESSHHHHHCC--- CS UPF0020 161 rlvlltsenkalekaleer 179 + + s + + ++a++ + tr|A0A1X7SY39|A0A1X7SY39_AMPQE 589 SAKYHKSMHDSYTRAIRWA 607 8888888887777777765 PP >> tr|A0A1X7SEZ0|A0A1X7SEZ0_AMPQE Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -2.0 0.0 1.1 5.9e+03 25 43 .. 57 75 .. 51 93 .. 0.75 2 ? 12.1 0.0 5.1e-05 0.28 80 128 .. 87 134 .. 77 135 .. 0.87 Alignments for each domain: == domain 1 score: -2.0 bits; conditional E-value: 1.1 T--TTS-EEETT-TTSHHH CS UPF0020 25 gwkpgepllDPmCGsGtil 43 ++ ++++ lD CG+G + tr|A0A1X7SEZ0|A0A1X7SEZ0_AMPQE 57 EIFKDKVVLDIGCGTGILS 75 5556777888889999765 PP == domain 2 score: 12.1 bits; conditional E-value: 5.1e-05 EEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STTS---EEEE- CS UPF0020 80 ygsDldrrvvqgareNaekagvgdliefsqadaakLrlkegevdvivtn 128 +++D + ++++a e a++ gv+d+i+f ++ ++rl+ ++vdv++++ tr|A0A1X7SEZ0|A0A1X7SEZ0_AMPQE 87 FAIDQ-SPIIHKAAEIARENGVDDKITFIRGEVESVRLPVDSVDVLISE 134 55554.45899999*********************************96 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (197 nodes) Target sequences: 43435 (14716620 residues searched) Passed MSV filter: 957 (0.0220329); expected 868.7 (0.02) Passed bias filter: 775 (0.0178428); expected 868.7 (0.02) Passed Vit filter: 55 (0.00126626); expected 43.4 (0.001) Passed Fwd filter: 8 (0.000184183); expected 0.4 (1e-05) Initial search space (Z): 43435 [actual number of targets] Domain search space (domZ): 8 [number of targets reported over threshold] # CPU time: 0.24u 0.01s 00:00:00.25 Elapsed: 00:00:00.11 # Mc/sec: 26356.13 // [ok]