# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.1b2 (February 2015); http://hmmer.org/
# Copyright (C) 2015 Howard Hughes Medical Institute.
# Freely distributed under the GNU General Public License (GPLv3).
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  pfam_list/UPF0020.hmm.txt
# target sequence database:        proteomes/Dictyostelium_discoideum.fasta
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       UPF0020  [M=197]
Accession:   PF01170.17
Description: Putative RNA methylase family UPF0020
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------               -----------
    3.1e-11   42.8   0.1    6.8e-09   35.2   0.1    3.5  2  sp|Q54BW1|Y3380_DICDI   Putative uncharacterized protein DDB_
    2.3e-07   30.2   0.2      0.016   14.5   0.0    2.6  3  sp|Q54QA6|TRM11_DICDI   tRNA (guanine(10)-N2)-methyltransfera
    9.2e-05   21.7   0.1     0.0037   16.5   0.1    2.2  1  tr|Q54VE3|Q54VE3_DICDI  Uncharacterized protein OS=Dictyostel
  ------ inclusion threshold ------
      0.011   15.0   0.1      0.031   13.5   0.1    1.8  1  sp|Q54WD6|TRM5_DICDI    tRNA (guanine(37)-N1)-methyltransfera
      0.014   14.6   0.1      0.056   12.6   0.0    2.0  2  tr|Q54NF6|Q54NF6_DICDI  Uncharacterized protein OS=Dictyostel
      0.015   14.5   0.0      0.058   12.6   0.0    2.0  2  tr|Q553T0|Q553T0_DICDI  Uncharacterized protein OS=Dictyostel


Domain annotation for each sequence (and alignments):
>> sp|Q54BW1|Y3380_DICDI  Putative uncharacterized protein DDB_G0293380 OS=Dictyostelium discoideum OX=44689 GN=DDB_G029
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !   35.2   0.1   3.2e-12   6.8e-09      10     138 ..     258     437 ..     249     457 .. 0.71
   2 ?    1.4   0.0     0.075   1.6e+02     139     185 ..     491     537 ..     487     545 .. 0.87

  Alignments for each domain:
  == domain 1  score: 35.2 bits;  conditional E-value: 3.2e-12
                            -SS-HHHHHHHHHH.........TT--TT.......S-EEETT-TTSHHHHHHHHHHTT--TTTT----SS................H CS
                UPF0020  10 apLketLAaalvrl.........agwkpg.......epllDPmCGsGtilIEaallganiapgllrefvle................l 65 
                            ap++etLA+a+++          +g ++g       + + DP+ GsGt+  Ea  +  ++ p    + +                   
  sp|Q54BW1|Y3380_DICDI 258 APIRETLASAIIMAiisstrrvgSGTTEGsyldlnkTNIWDPFVGSGTLIQEAISMLLKAKPSSCTNYKRSfqferfknhsterylkY 345
                            6666666666665333322333344444411111115799**************999999886553333224557******9998777 PP

                            HHHHHHHHHHH--...EEEEES-HHHHHHHHHHHHHTT-GG.GEEEEE--GGG--...............STTS---EEEE------- CS
                UPF0020  66 kaeaeeearaelk...lygsDldrrvvqgareNaekagvgd.liefsqadaakLr...............lkegevdvivtnpPYGer 134
                            ++ +++ + + +k   l+gsD+d+++++ +  N++k g ++  i+f + d++++                +++++  +i+tn+PYG r
  sp|Q54BW1|Y3380_DICDI 346 LESINKPISPLIKntiLIGSDIDQKAIDSSIFNLSKQGYQHeNIKFFKGDFKSIYldqinidnsldddgdNNNNKPFTIITNLPYGTR 433
                            7777777775555667*********************9976269****9*9998766776667666665533444459*********9 PP

                            ---H CS
                UPF0020 135 lgsk 138
                            + + 
  sp|Q54BW1|Y3380_DICDI 434 VLNR 437
                            8655 PP

  == domain 2  score: 1.4 bits;  conditional E-value: 0.075
                            HHHHHHHHHHHHHHHHH-TT..-EEEEEESSHHHHHCC---ESEEEE CS
                UPF0020 139 kalekLYseflrelkrvlrgggrlvlltsenkalekaleeralrkvk 185
                            k+l++L++ f++ +++  ++  r v+++++n+ ++++  ++  ++++
  sp|Q54BW1|Y3380_DICDI 491 KDLRDLFKRFGDMIHESNESVLRDVFVLNGNSHFQSISTNNGKNNFE 537
                            67899***********999999*************999888776665 PP

>> sp|Q54QA6|TRM11_DICDI  tRNA (guanine(10)-N2)-methyltransferase homolog OS=Dictyostelium discoideum OX=44689 GN=trmt11
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ?   -3.8   0.0       2.8     6e+03     143     167 ..      84     108 ..      81     131 .. 0.64
   2 !   13.0   0.0   2.1e-05     0.045       4      54 ..     206     256 ..     204     283 .. 0.87
   3 !   14.5   0.0   7.3e-06     0.016     119     139 ..     302     322 ..     292     328 .. 0.87

  Alignments for each domain:
  == domain 1  score: -3.8 bits;  conditional E-value: 2.8
                            HHHHHHHHHHHHH-TT..-EEEEEE CS
                UPF0020 143 kLYseflrelkrvlrgggrlvllts 167
                            +L +e+l+el++ ++++  + ++++
  sp|Q54QA6|TRM11_DICDI  84 QLLDEILNELHSKFDKQFLSNYMIN 108
                            6777888888888877544444444 PP

  == domain 2  score: 13.0 bits;  conditional E-value: 2.1e-05
                            S-SS---SS-HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHHHTT-- CS
                UPF0020   4 RefngpapLketLAaalvrlagwkpgepllDPmCGsGtilIEaallgania 54 
                            R++ g +++ ++L+ +  ++  +k+g+  lDP+ G+G++ + a   ga+ +
  sp|Q54QA6|TRM11_DICDI 206 RKYLGTTSMDPELSLVSANMGLVKKGHFVLDPFVGTGSFILVASHFGAQTV 256
                            889999*********************************998887777665 PP

  == domain 3  score: 14.5 bits;  conditional E-value: 7.3e-06
                            TS---EEEE----------HH CS
                UPF0020 119 egevdvivtnpPYGerlgskk 139
                            ++ +d+i+t+pPYG+r g+ k
  sp|Q54QA6|TRM11_DICDI 302 NSMFDSIITDPPYGIRAGARK 322
                            5679************99865 PP

>> tr|Q54VE3|Q54VE3_DICDI  Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0205190 PE=4 SV=1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !   16.5   0.1   1.7e-06    0.0037      77     114 ..      70     107 ..      30     116 .. 0.77

  Alignments for each domain:
  == domain 1  score: 16.5 bits;  conditional E-value: 1.7e-06
                             --EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG CS
                 UPF0020  77 lklygsDldrrvvqgareNaekagvgdliefsqadaak 114
                             ++ +g+D+++++++ a+eNa+k gv++l+ f++ d+a+
  tr|Q54VE3|Q54VE3_DICDI  70 IRGIGLDINPELIKSANENAKKEGVDHLVLFKVQDFAN 107
                             3458*****************************99874 PP

>> sp|Q54WD6|TRM5_DICDI  tRNA (guanine(37)-N1)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=trmt5 PE=3 SV=1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ?   13.5   0.1   1.5e-05     0.031      75     130 ..     235     294 ..     207     296 .. 0.85

  Alignments for each domain:
  == domain 1  score: 13.5 bits;  conditional E-value: 1.5e-05
                           HH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--....STTS---EEEE--- CS
               UPF0020  75 aelklygsDldrrvvqgareNaekagvgdliefsqadaakLr....lkegevdvivtnpP 130
                           ++ k+y+ Dl+++ v+  +eNa+  +++ ++e+s++da+++     +k+  +  +v n+P
  sp|Q54WD6|TRM5_DICDI 235 KKCKVYANDLNPSSVKYMKENAKTNRLESKVEISNLDARDFVkslvEKSIPFTHVVMNLP 294
                           66789**********************************998665444556777777777 PP

>> tr|Q54NF6|Q54NF6_DICDI  Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0186431 PE=4 SV=1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ?   -3.2   0.0       1.9   4.1e+03      77     108 ..     247     280 ..     224     291 .. 0.69
   2 ?   12.6   0.0   2.6e-05     0.056      29     119 ..     324     408 ..     313     417 .. 0.75

  Alignments for each domain:
  == domain 1  score: -3.2 bits;  conditional E-value: 1.9
                             --EEEEES-HHHHHHHHHHHHH..TT-GGGEEEE CS
                 UPF0020  77 lklygsDldrrvvqgareNaek..agvgdliefs 108
                             +++ g   ++  + +a +N ++  a+ ++ i fs
  tr|Q54NF6|Q54NF6_DICDI 247 AEISGCKVIWHKIMTAHSNISNnkATFTHMICFS 280
                             4577777788888888888654115555555555 PP

  == domain 2  score: 12.6 bits;  conditional E-value: 2.6e-05
                             TS-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEE.S-HHHHHHHHHHHHHTT-GGGEEEEE--GGG CS
                 UPF0020  29 gepllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsD.ldrrvvqgareNaekagvgdliefsqadaak 114
                              ++++DP+CG G++l  a + g + +   l         + +++++ +l +  ++ +d+rv q++++N ++  ++d  ++++ d  +
  tr|Q54NF6|Q54NF6_DICDI 324 STTIIDPFCGKGSVLAVANVYGLDSVGVDL------SSGKTKNSFNLQLDVETVNkFDPRVWQQSNNNKSNLLIKDNDNIEDNDESD 404
                             579************999999999993222......334445555555555555526899999999999999999999999999999 PP

                             --STT CS
                 UPF0020 115 Lrlke 119
                             ++ ++
  tr|Q54NF6|Q54NF6_DICDI 405 IT-NN 408
                             88.33 PP

>> tr|Q553T0|Q553T0_DICDI  Uncharacterized protein OS=Dictyostelium discoideum OX=44689 GN=DDB0202574 PE=3 SV=1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ?   -1.6   0.0      0.62   1.3e+03     139     173 ..     134     167 ..     127     190 .. 0.63
   2 ?   12.6   0.0   2.7e-05     0.058      77     125 ..     263     311 ..     233     362 .. 0.84

  Alignments for each domain:
  == domain 1  score: -1.6 bits;  conditional E-value: 0.62
                             HHHHHHHHHHHHHHHHH-TT..-EEEEEESSHHHH CS
                 UPF0020 139 kalekLYseflrelkrvlrgggrlvlltsenkale 173
                             +++ + +++fl+++k+ +++ g +v +++++  ++
  tr|Q553T0|Q553T0_DICDI 134 REMTHTWNAFLESIKKTVET-GSIVKIAPQEDVVK 167
                             55556678899999998888.66666666555544 PP

  == domain 2  score: 12.6 bits;  conditional E-value: 2.7e-05
                             --EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STTS---EE CS
                 UPF0020  77 lklygsDldrrvvqgareNaekagvgdliefsqadaakLrlkegevdvi 125
                              k++g++++++ v  a + a+  gv+d+ +f   da k++ +++ +dv+
  tr|Q553T0|Q553T0_DICDI 263 CKIRGLNINKKQVGIATQRAKDLGVSDRASFDHGDAMKMPYPDNTFDVV 311
                             5799************************************999999987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                              1  (197 nodes)
Target sequences:                        12739  (6856946 residues searched)
Passed MSV filter:                       441  (0.0346181); expected 254.8 (0.02)
Passed bias filter:                      270  (0.0211948); expected 254.8 (0.02)
Passed Vit filter:                        26  (0.00204098); expected 12.7 (0.001)
Passed Fwd filter:                         6  (0.000470995); expected 0.1 (1e-05)
Initial search space (Z):              12739  [actual number of targets]
Domain search space  (domZ):               6  [number of targets reported over threshold]
# CPU time: 0.12u 0.01s 00:00:00.13 Elapsed: 00:00:00.05
# Mc/sec: 27016.37
//
[ok]