# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/UPF0020.hmm.txt # target sequence database: proteomes/Ornithorhynchus_anatinus.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: UPF0020 [M=197] Accession: PF01170.17 Description: Putative RNA methylase family UPF0020 Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.4e-46 157.7 0.0 5e-46 157.1 0.0 1.2 1 tr|F6WCE9|F6WCE9_ORNAN THUMP domain containing 3 OS=Ornithor 1.8e-14 54.2 0.9 1.5e-13 51.2 0.9 2.2 1 tr|F6ZAJ2|F6ZAJ2_ORNAN Uncharacterized protein OS=Ornithorhy 1.8e-10 41.1 0.0 3.9e-10 40.0 0.0 1.5 1 tr|F7AMQ3|F7AMQ3_ORNAN tRNA methyltransferase 11 homolog OS= 8.9e-08 32.3 0.1 7.9e-07 29.2 0.1 2.1 1 tr|F6R9A4|F6R9A4_ORNAN Methyltransferase like 5 OS=Ornithorh 2.2e-05 24.5 0.0 8.6e-05 22.6 0.0 1.8 1 tr|F6YXL2|F6YXL2_ORNAN Trimethylguanosine synthase 1 OS=Orni 0.0078 16.2 0.0 0.018 15.0 0.0 1.7 2 tr|F7CZD8|F7CZD8_ORNAN 2-methoxy-6-polyprenyl-1,4-benzoquino ------ inclusion threshold ------ 0.015 15.3 0.0 0.37 10.7 0.0 2.2 2 tr|F7DN36|F7DN36_ORNAN Uncharacterized protein OS=Ornithorhy Domain annotation for each sequence (and alignments): >> tr|F6WCE9|F6WCE9_ORNAN THUMP domain containing 3 OS=Ornithorhynchus anatinus OX=9258 GN=THUMPD3 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 157.1 0.0 1.6e-49 5e-46 7 196 .. 218 397 .. 212 398 .. 0.96 Alignments for each domain: == domain 1 score: 157.1 bits; conditional E-value: 1.6e-49 S---SS-HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHHHHH CS UPF0020 7 ngpapLketLAaalvrlagwkpgepllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrvvqgar 93 +gp++L++tLA++++rl+ + g++++DPmCGsG+i IE+ + n+++++ ++++ + ++s ++++++++ tr|F6WCE9|F6WCE9_ORNAN 218 FGPTTLRSTLAYGMLRLCSPQLGDVIVDPMCGSGAISIEGICEWPNCYHIAG-------------DNNPLAVKRASSNIWSLMTRNS 291 67**********************************************7666.............88899999************** PP HHHHHTT-GGGEEEEE--GGG--STTS---EEEE----------HHHHHHHHHHHHHHHHHH-TT...-EEEEEESSHHHHHCC--- CS UPF0020 94 eNaekagvgdliefsqadaakLrlkegevdvivtnpPYGerlgskkalekLYseflrelkrvlrgg.grlvlltsenkalekaleer 179 ++ +k ++g ++ q+d+++L+l++++vd+iv+++P+G+r+gs k++++LY+ +l+e++rv++ g gr+vllt+++k++ kal+++ tr|F6WCE9|F6WCE9_ORNAN 292 TKERKPTIGLPVDSIQWDICNLPLRTSSVDIIVSDMPFGKRIGSRKKNWDLYPFCLQEMSRVCKPGtGRAVLLTQDRKCFIKALSKM 378 ***********************999**************************************999******************** PP ..ESEEEEEEETTEEEEEE CS UPF0020 180 ..alrkvkeknvklglllk 196 +rkv+ +v++g+l++ tr|F6WCE9|F6WCE9_ORNAN 379 cdVWRKVQTAWVNIGGLHA 397 *9*************9985 PP >> tr|F6ZAJ2|F6ZAJ2_ORNAN Uncharacterized protein OS=Ornithorhynchus anatinus OX=9258 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 51.2 0.9 4.8e-17 1.5e-13 4 109 .. 270 351 .. 268 358 .. 0.94 Alignments for each domain: == domain 1 score: 51.2 bits; conditional E-value: 4.8e-17 S-SS---SS-HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHH CS UPF0020 4 RefngpapLketLAaalvrlagwkpgepllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrvvq 90 R + + a L++t+A a+ la++++g+ lDPmCG Gtil+Eaa + ++ g D+ ++ +q tr|F6ZAJ2|F6ZAJ2_ORNAN 270 RTYIKTAGLRSTIAWAMASLAEINAGSLVLDPMCGLGTILLEAAKEWPDAN------------------------YLGTDISNSQLQ 332 888999******************************************999........................79********** PP HHHHHHHHTT-GGGEEEEE CS UPF0020 91 gareNaekagvgdliefsq 109 ar+N ++ag+ d+ie+ + tr|F6ZAJ2|F6ZAJ2_ORNAN 333 VARDNVNAAGLMDKIELLK 351 **************99765 PP >> tr|F7AMQ3|F7AMQ3_ORNAN tRNA methyltransferase 11 homolog OS=Ornithorhynchus anatinus OX=9258 GN=TRMT11 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 40.0 0.0 1.3e-13 3.9e-10 3 140 .. 191 321 .. 190 333 .. 0.86 Alignments for each domain: == domain 1 score: 40.0 bits; conditional E-value: 1.3e-13 SS-SS---SS-HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHH CS UPF0020 3 yRefngpapLketLAaalvrlagwkpgepllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrvv 89 +R+f g++++ + LA + + ++k++++ +DP+ G+G++l+ a ga++ + ++ + + +++r+++k +g D + tr|F7AMQ3|F7AMQ3_ORNAN 191 KRHFIGNTSMDAGLAFIMTNHGRVKENDVVFDPFVGTGGLLVASAHFGAYVC---GTDIDYNTVHGLGKATRRNQKWRGPDENI--- 271 79**************************************************...5667888888999999999*******876... PP HHHHHHHHHTT-GGG.EEEEE--GGG--.STTS---EEEE----------HHH CS UPF0020 90 qgareNaekagvgdl.iefsqadaakLr.lkegevdvivtnpPYGerlgskka 140 r N++++g+++ ++ + da k +k +++i+t+pPYG+r ++ k+ tr|F7AMQ3|F7AMQ3_ORNAN 272 ---RANLRQYGLEKYyLDVLVSDASKPSwRKGMFFNTILTDPPYGIRESTRKT 321 ...57999999998624566667766664355589************887765 PP >> tr|F6R9A4|F6R9A4_ORNAN Methyltransferase like 5 OS=Ornithorhynchus anatinus OX=9258 GN=METTL5 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 29.2 0.1 2.6e-10 7.9e-07 28 139 .. 51 137 .. 25 175 .. 0.75 Alignments for each domain: == domain 1 score: 29.2 bits; conditional E-value: 2.6e-10 TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG CS UPF0020 28 pgepllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrvvqgareNaekagvgdliefsqadaak 114 ++++ +D CG G + I a+lga +g+D+d+ +++ +N e+ ++++ i++ q ++++ tr|F6R9A4|F6R9A4_ORNAN 51 EDKVVADLGCGCGVLSIGSAMLGAGLC-------------------------VGFDIDEDALEIFSRNVEEFELTN-IDMIQSNVCS 111 455666666777777777777777666.........................8********************986.788888**** PP --.STTS---EEEE----------HH CS UPF0020 115 Lr.lkegevdvivtnpPYGerlgskk 139 L+ ++++++d+++ npP+G + ++ tr|F6R9A4|F6R9A4_ORNAN 112 LPdRMSKSFDTVIMNPPFGTKHNKGM 137 **99999************8766554 PP >> tr|F6YXL2|F6YXL2_ORNAN Trimethylguanosine synthase 1 OS=Ornithorhynchus anatinus OX=9258 GN=TGS1 PE=4 SV=2 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 22.6 0.0 2.8e-08 8.6e-05 33 132 .. 620 692 .. 615 694 .. 0.86 Alignments for each domain: == domain 1 score: 22.6 bits; conditional E-value: 2.8e-08 EETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STT CS UPF0020 33 lDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrvvqgareNaekagvgdliefsqadaakLrlke 119 +D +CG G+ +I al g++++ ++D+d+ ++ ar+Na+ +gv+d+ e+ + d+ L ++ tr|F6YXL2|F6YXL2_ORNAN 620 VDAFCGVGGNAIQFALAGKRVI--------------------------AIDIDPVKIDLARNNAQVYGVADRLELICGDFLLLA-PN 679 5666666666666666666666..........................9********************************999.99 PP S---EEEE----- CS UPF0020 120 gevdvivtnpPYG 132 + dv+ pP+G tr|F6YXL2|F6YXL2_ORNAN 680 LKADVVFLSPPWG 692 9***********9 PP >> tr|F7CZD8|F7CZD8_ORNAN 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial OS=Ornithorhynchus anatinus O # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 15.0 0.0 5.7e-06 0.018 26 124 .. 104 213 .. 97 243 .. 0.66 2 ? -4.0 0.0 3.8 1.2e+04 167 186 .. 293 312 .. 291 318 .. 0.77 Alignments for each domain: == domain 1 score: 15.0 bits; conditional E-value: 5.7e-06 --TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SS........HHHHHHHHHH.......HH--EEEEES-HHHHHHHHHHHH CS UPF0020 26 wkpgepllDPmCGsGtilIEaallganiapgllrefvle........lkaeaeeear.......aelklygsDldrrvvqgareNae 97 +pg++llD G+G i+ ++ ++ re+ + ++ + +++ D+++++++ ++e+a+ tr|F7CZD8|F7CZD8_ORNAN 104 PHPGTQLLDVAGGTGDIAFRFINYIRSQQRQQQREKLKAqqnlswaeI----AKSYQgvahdplGGSQVVVCDINKEMLKVGKEKAQ 186 556666666666666665443333333332333332222554433332....22222322233334458999*************** PP HTT-GGGEEEEE--GGG--STTS---E CS UPF0020 98 kagvgdliefsqadaakLrlkegevdv 124 g g+ ++++ da +L+ +++++dv tr|F7CZD8|F7CZD8_ORNAN 187 TLGYGKGLAWVVGDAEELPFDDDKFDV 213 *******************88778886 PP == domain 2 score: -4.0 bits; conditional E-value: 3.8 ESSHHHHHCC---ESEEEEE CS UPF0020 167 senkalekaleeralrkvke 186 ++++++++++e++ +rkv+ tr|F7CZD8|F7CZD8_ORNAN 293 PAQEEFKEMIEDAGFRKVTY 312 67788999999999998875 PP >> tr|F7DN36|F7DN36_ORNAN Uncharacterized protein OS=Ornithorhynchus anatinus OX=9258 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.7 0.0 0.00012 0.37 92 154 .. 150 215 .. 148 241 .. 0.86 2 ? 2.1 0.0 0.054 1.7e+02 113 154 .. 337 381 .. 318 409 .. 0.74 Alignments for each domain: == domain 1 score: 10.7 bits; conditional E-value: 0.00012 HHHHHHHTT-GGGEEEEE--GGG--...STTS---EEEE----------HHHHHHHHHHHHHHHHH CS UPF0020 92 areNaekagvgdliefsqadaakLr...lkegevdvivtnpPYGerlgskkalekLYseflrelkr 154 ar+ ++ + +++++++++++ ++ ++++ + +i+++ P+ e+l + +++++LY++++++l + tr|F7DN36|F7DN36_ORNAN 150 ARKEVKSWEEQEQWDVEDMKVSGVHwgyEETKTFLAILGEAPFSEKLRTCHQNRHLYRAIAERLRE 215 667777777788889999999999999988889***************************999877 PP == domain 2 score: 2.1 bits; conditional E-value: 0.054 GG--...STTS---EEEE----------HHHHHHHHHHHHHHHHH CS UPF0020 113 akLr...lkegevdvivtnpPYGerlgskkalekLYseflrelkr 154 a ++ ++++ + i+++ P+ e+l + +++ + Y++++++l tr|F7DN36|F7DN36_ORNAN 337 AGVHwgyEETKTFLGILGEAPFSEKLRTCHQNSQVYRAIAERLRA 381 55555555566788899*********************9988765 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (197 nodes) Target sequences: 21677 (8259262 residues searched) Passed MSV filter: 554 (0.025557); expected 433.5 (0.02) Passed bias filter: 452 (0.0208516); expected 433.5 (0.02) Passed Vit filter: 39 (0.00179914); expected 21.7 (0.001) Passed Fwd filter: 7 (0.000322923); expected 0.2 (1e-05) Initial search space (Z): 21677 [actual number of targets] Domain search space (domZ): 7 [number of targets reported over threshold] # CPU time: 0.14u 0.01s 00:00:00.15 Elapsed: 00:00:00.06 # Mc/sec: 27117.91 // [ok]